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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... The ADP-ribosylation reactions contained 0.2 μM purified PARP-1 or PARP-3 and 100 ng/μL of sheared salmon sperm DNA (Invitrogen, AM9680). Purified proteins were mixed with the indicated amounts of NAD+ in ADP-ribosylation Buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2022Quote: ... and dialyzed against 1 L of PB in a 3 ml Slide-A-Lyzer™ Dialysis Cassettes (Thermo Fisher Scientific) at RT for 3 hrs ...
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bioRxiv - Immunology 2022Quote: ... Heat inactivated serum samples (56°C for 1 hour) were serially diluted (3-fold) in minimum essential media (MEM; Gibco) supplemented with 2 mM L-glutamine (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... Epithelial cell culture medium containing Y-27632 consisted of DMEM/F12 in a 3:1 ratio containing 1x penicillin–streptomycin (Gibco), 5% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2021Quote: Zebrafish larvae at 3 to 14 dpf were anesthetized with tricaine and mounted laterally in 1% low melting point agarose (ThermoFisher) on a glass bottom dish (#1.5 cover glass ...
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bioRxiv - Microbiology 2021Quote: HEK293T-ACE2 cells were reverse-transfected with plasmids and TransIT-293 at a ratio of 1 µg DNA : 3 µLTransit-T293 in Opti-MEM (Gibco). For an experiment conducted in triplicate at a 48-well scale ...
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bioRxiv - Cell Biology 2021Quote: INS-1 832/3 cells (a gift from Professor Christopher Newgard, Duke University, USA) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were then washed 3 times with PBS and incubated with Alexa Fluor 488-conjugated anti-rabbit secondary antibodies (1:10000; A32731; Invitrogen) for 1 h at room temperature to visualize the antibody reactions ...
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bioRxiv - Neuroscience 2019Quote: ... primary cortical neurons were transfected with shNeurod2-1 (Guner et al., 2017) at 3 DIV with Lipofectamine 2000 transfection reagent (Invitrogen). Neurons were fixed with 4% PFA at 5 DIV ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected according to manufacturer’s instructions using 1 µg of total DNA complexed with 3 µg Lipofectamine2000 (ThermoFisher 11668019) in 200 µL Opti-MEM (ThermoFisher 51985091) ...
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bioRxiv - Bioengineering 2020Quote: ... Attached brain organoids were then incubated on coverslips for 3 days under DMSO vehicle or THC (1 μM) treatment and stained with celltracker green (Invitrogen) to visualize neurons ...
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bioRxiv - Biochemistry 2019Quote: ... followed by a 3-min incubation with the same buffer additionally containing 50 μg mL-1 of NeutrAvidin (Life Technologies). The chamber was subsequently washed with γTuRC storage buffer and incubated for 5 min with prediluted γTuRC in γTuRC storage buffer to the concentration indicated for each experiment ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were routinely propagated on 0.1% gelatin-coated plastic and replated every 3 days at a split ratio of 1 in 10 following dissociation with Accutase (Gibco) as previously reported [9].
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bioRxiv - Microbiology 2020Quote: ... Each dish was transfected with 2 μg of pCDNA 3.1 Venus plasmid using the using a 3:1 ratio of plasmid to transfection reagent in Opti-MEM (Gibco, 31985062) and a total final volume of 200 μl ...
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bioRxiv - Immunology 2021Quote: ... 2×108 spleen cells and inguinal lymph node cells (about 1×108 B cells) were mixed with 108 Sp2/0 cells and washed 3 times with RPMI1640 medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... or 1×104 Calu-3 (12 μl/well) were seeded in 384-well white optical-bottom tissue culture-treated plates (Nunc) with a Multidrop Combi liquid handling instrument (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were washed three times with PBS and secondary antibodies diluted in 3% BSA in PBS (1:1000; Invitrogen) were incubated for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: RNA was isolated from the aqueous phase of homogenized spleens mixed with 1-bromo-3-chloropropane and purified with the PureLink RNA kit (Invitrogen) separately ...
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bioRxiv - Biochemistry 2021Quote: ... that were hydrated for 10 min in assay buffer then plunged in a solution containing 1 μM 3′ end–biotinylated TAR RNA in assay buffer with 1x RNAase inhibitor (RNA Secure, Invitrogen) and 0.1 μM BSA for the RNA loading step ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3 washes with PBS and incubation with secondary antibody (Alexa Fluor 488 goat anti-chicken, 1:1000; Invitrogen) on a slow rocker at room temperature for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... These prepared samples were loaded on 3–12% Bis-Tris Native PAGE gels and electrophoresed using 1×Native PAGE Running buffer system (Invitrogen). The cathode buffer included 1×Cathode Buffer Additive (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen) running buffer ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed 3 times in the blocking solution and incubated in Alexa Fluor 594-conjugated (red) goat secondary antibody (1:500; Invitrogen) for 2 hr.
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bioRxiv - Immunology 2020Quote: ... Plate was washed for 3 times with DPBS + 0.05 % Tween 20 (Scharlau, Spain) and then blocked with PBS + 1 % BSA (ThermoFisher, USA) + 0.050 % Tween 20 for 2 hours at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: Colon ulcers and distant normal colon tissue were excised from biopsy-wounded wild-type and Vil1-Cre;Rosa26Bmp4 mice at 1 day and 3 days post-wounding and stored in RNAlater (ThermoFisher) at −80°C prior to extraction ...
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Evaluation of the OsTIR1 and AtAFB2 AID systems for chromatin protein degradation in mammalian cellsbioRxiv - Molecular Biology 2021Quote: ... established in our laboratory [24]) were cultured on a gelatin surface under 2i condition (1 μM PD, 3 μM CHIR) in DMEM (ThermoFisher), supplemented with 7.5% ES FBS (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... slices were washed 2-3 times in 0.1 M PB then transferred to a solution containing Streptavidin conjugated to AlexaFluor568 (1:500, Invitrogen, UK) vand 0.3% Triton X-100 and 0.05% NaN3 ...
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bioRxiv - Immunology 2022Quote: ... The sorted cells were then incubated with Totalseq-C antibodies (1:50 dilution) on ice for 30 min, washed 3 times (by default, top up with 2% FBS (Gibco) in DPBS (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... mandibular alveolar bone tissue was cut into small pieces (<1 mm3) and digested with 3 mg/mL collagenase type I (Gibco) and 4 g/mL dispase II (Sigma ...
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bioRxiv - Immunology 2022Quote: ... for 3 min and incubated in 1% phosphomolybdic acid then acetic acid solution (A38C-212; Thermo Fisher Scientific, Waltham, MA) for 5 min and 3 min ...
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bioRxiv - Immunology 2022Quote: The EV-enriched fractions (1-3) were combined and concentrated using Amicon Ultra 0.5mL 30kDa cutoff spin columns (Cat. No. 10012584, Fisher Scientific). 300 mesh continuous carbon support copper grids (Cat ...
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bioRxiv - Microbiology 2022Quote: ... The next day, the larvae were washed (3×, 1× PBST) and incubated for 30 minutes in 2 μg/mL Hoechst 33342 (Invitrogen) in 1× PBT at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... HEK-293T cells were transfected with plasmids encoding the WNV replicon and ZIKV structural genes at a 1:3 ratio by mass using Lipofectamine 3000 (Invitrogen), followed by incubation at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were co-cultured with HLA-B/CMV-restricted CD8+ T-cells for five hours (E:T ratio: 3:1) in RPMI 1640 medium (Life Technologies)(supplemented with 10 % (v/v ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... cortical astrocytes were dissected from SD neonatal rats (1-3 days) with the aseptic operation and digested by 0.25% trypsin (Gibco, USA) at 37°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed 3 times and incubated with 1/1000 of monoclonal secondary antibody conjugated to Alexa Fluor 594 (Invitrogen) for 45 min at RT in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... These prepared samples were loaded onto 3–12% Bis-Tris Native PAGE gels and electrophoresed using a 1× native PAGE running buffer system (Invitrogen). The cathode buffer included 1× cathode buffer additive (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... for 20 min at RT and incubated with 3% NGS PBS containing secondary antibodies (1:600, Alexa-Fluor 594 anti-mouse IgG, Invitrogen and Alexa-Fluor 488 anti-guinea pig IgG ...
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bioRxiv - Neuroscience 2024Quote: HAP1 WT and SNCA KO cells were seeded for ∼75% confluency and transfected with NHEJ and HR plasmid reporters51 using X-tremeGENE HP DNA Transfection Reagent (1:3 DNA:reagent) in OptiMEM (Gibco #31985062). Images were taken on a Zeiss Axio Observer.D1 outfitted with an Excelitas X-Cite 120 LED GFP light at 24 ...
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bioRxiv - Biochemistry 2024Quote: ... cartilage shavings were submerged in 3:1 methanol:water and homogenized using a tissue homogenizer (SPEX Sample Prep 1200 GenoLyte, Fisher Scientific). Homogenization included 15 cycles of homogenizing for 20 seconds and resting for 2 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA-treated cells were washed twice with 37°C M1 and replaced with M1 containing 1 μM TO-PRO-3 iodide (ThermoFisher) or washed once with 37°C M2 containing 5 mM ethylene glycol-bis(2-aminoethylether)-N ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 µL of 1 mM TO-PRO-3 Iodide in DMSO (Thermo) was added to stain dead cells and 1uL of DRAQ5 (Invitrogen) was added to stain viable cells ...
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bioRxiv - Genomics 2024Quote: ... and nuclei were washed once with nuclei suspension buffer (NSB; 10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% Superase RNA Inhibitor (Thermo Fisher), 1% 0.2mg/mL Ultrapure BSA (New England Biosciences)) ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed 3 times with 1X PBS and treated with 1 μM C11-Bodipy 581/591 dye (Invitrogen) suspended in 1X PBS for 30 min in the dark at 37oC ...
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bioRxiv - Cell Biology 2024Quote: TRIM28 and TRIM24 knockdowns were performed similarly with the following modifications: 3×106 TRExRTA BCBL-1 cells in 100 μL of resuspension buffer T (Invitrogen) were nucleofected once with 2 uL of 100 μM of either Dharmacon human TRIM28 Accell SmartPool siRNA or OnTarget Plus human TRIM24 SmartPool ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were detected using Alexa Fluor 488 labeled goat anti-rabbit IgG antibodies diluted 1:1000 in PBS containing 3% BSA (Invitrogen). Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted in 100 µL of nuclease free water and precipitated at -80 °C for ≥ 3 hours with 1 µL of 15 mg/mL GlycoBlue coprecipitant (ThermoFisher), 10 µL of 3 M sodium acetate pH 5.5 ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Microbiology 2022Quote: ... isolated primary cells were co-cultivated with gamma-irradiated mitotically inactivated NIH3T3 mouse embryonic fibroblasts (MEFs) in a 3:1 mixture of Ham’s F-12 nutrient mix (Life technologies) and DMEM supplemented with 5 % FCS ...