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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... was used to lyse cell pellets and protein concentration determined using the Micro BCA Protein Assay kit (ThermoFisher Scientific, 232350). 10 μg protein ...
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bioRxiv - Neuroscience 2022Quote: ... Both the protein extracts from cell pellets and the supernatants from cultured cells were subjected to protein quantification using BCA Protein Assay Kit (Thermofisher). Samples were boiled at 99 °C for 2 min ...
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bioRxiv - Microbiology 2022Quote: ... Library concentration was measured with a Qubit 2.0 Fluorometer using a dsDNA BR Assay Kit (Life Technologies, Carlsbad, CA, USA). All 48 libraries were pooled in an equimolar fashion and submitted to Genewiz (South Plainfield ...
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bioRxiv - Cell Biology 2022Quote: ... We measured the RNA integrity using Agilent RNA screentape and RNA concentration using Qubit RNA HS assay kit (ThermoFisher Scientific). We used 1.2 µg total RNA for mRNA purification ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were quantified using the Qubit 4.0 fluorometer using the 1X dsDNA HS Assay Kit (Thermo Fisher Scientific, USA) and sequencing libraries prepared using the Nextera DNA Flex Prep kit (Illumina ...
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bioRxiv - Genetics 2022Quote: ... and a Qubit 4 Fluorometer with the Quant-iT™ Qubit™ dsDNA BR Assay Kit (Thermo Scientific, Wilmington, DE). DNA concentrations can be found in Table S2.
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bioRxiv - Genetics 2022Quote: ... DNA concentrations for each sample were determined with the Qubit dsDNA BR assay kit (Thermo Fisher Scientific, Waltham, MA, USA) in a 96 well format using a Synergy H1 plate reader (BioTek Instruments ...
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bioRxiv - Genomics 2022Quote: The amplicon yields of the 2nd-round WGA were quantified by a Qubit dsDNA HS assay kit (Thermo Fisher Scientific). For model bacteria ...
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bioRxiv - Cancer Biology 2022Quote: ... After column-based isolation of the amplicon and quantification of DNA-yield using a Qubit 3.0 fluorometer and the dsDNA HS assay kit 392 (Thermo Fisher), paired-end sequencing was performed on an Illumina Miseq ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysed supernatant was obtained by centrifugation at 4°C for 10 min at 13,000 rpm and the total protein content was quantified by a Micro BCA Protein assay kit (Thermo Scientific). The proteins were boiled in Laemmli SDS sample buffer (Alfa Aesar ...
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bioRxiv - Neuroscience 2022Quote: ... was used and the concentration was measured by a QubitR dsDNA HS Assay kit (Thermo Fisher Scientific Inc., Waltham, USA). The concentration was adjusted to 2 nM prior to sequencing (50 bp ...
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bioRxiv - Immunology 2022Quote: ... Purified RNA was assessed for mass concentration using the Qubit RNA BR Assay kit (catalog # Q10211; Invitrogen, Waltham, MA, USA) and for integrity using the Standard Sensitivity RNA Analysis kit on a Fragment Analyzer capillary electrophoresis system (catalog # DNF-471-0500 ...
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bioRxiv - Physiology 2022Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
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bioRxiv - Genomics 2022Quote: ... We also quantified the RNA using a Qubit RNA High Sensitivity Assay Kit (Invitrogen™, Thermo Fisher Scientific, Waltham, MA) assayed on a Qubit 4 Fluorometer (Invitrogen™ ...
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bioRxiv - Genomics 2022Quote: We diluted the RNA samples to 1 ng/μL for library preparation and confirmed the concentration using a Qubit RNA High Sensitivity Assay Kit (Invitrogen™ ...
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bioRxiv - Neuroscience 2022Quote: smFISH for miRNA detection on hippocampal neuron cultures was performed using the QuantiGene ViewRNA miRNA Cell Assay kit (Thermo Fisher) according to the manufacturer’s protocol with slight modifications ...
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bioRxiv - Immunology 2022Quote: ... we quantified fluorescence after PI staining or lactate dehydrogenase release using the CyQuant LDH cytotoxicity assay kit from Thermo Fisher. Briefly ...
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bioRxiv - Immunology 2022Quote: ... All products were checked on an Agilent bioanalyzer High Sensitivity DNA kit (Diagnostech) and Qubit dsDNA HS assay (ThermoFisher Scientific) and cleaned-up using 0.75X Ampure Beads (Beckman-Coulter) ...
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bioRxiv - Cell Biology 2022Quote: Click-iT Plus EdU Flow Cytometry Assay Kits Alexa Fluor 488 and FxCycle PI/RNase Staining Solution were obtained from Invitrogen to define the ground-truth of the cell cycle stages (G1 ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... The quantity and quality of the DNA were measured using a Qubit dsDNA HS Assay kit (Q32851; Thermo Fisher Scientific) and a spectrophotometer (NanoDrop 2000 ...
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bioRxiv - Biophysics 2022Quote: ... The concentration of the protein was determined using the Pierce BCA Protein Assay Kit (cat. No. 23225; Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Extracted protein from the cell-only control samples was quantified using Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher). 10 µg protein from each sample was then analyzed by sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE ...
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: The protein concentration of the proteoliposomes was measured with the Micro BCATM Protein Assay Kit (Thermo Fisher Scientific, Prod. #23235). SDS-PAGE and silver staining verified protein purity ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
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bioRxiv - Developmental Biology 2022Quote: Protein synthesis was detected by the Click-iT® Plus OPP Alexa Fluor 594 Protein Synthesis Assay Kit (Molecular Probes) as previously described (Sanchez et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was eluted in EB buffer and quantified using Qubit 2.0 fluorometer with the DNA BR assay kit (Life Technologies).
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured with BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Fisher Scientific, Carlsbad, CA, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were then purified using 0.6 x VAHTSTM DNA Clean Beads and were quantified by Qubit dsDNA HS assay kit (Invitrogen, Q32854).
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bioRxiv - Microbiology 2020Quote: ... DNA concentration was measured through fluorometry using a Qubit dsDNA HS Assay Kit with a Qubit 2.0 fluorometer (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... Protein concentrations were determined using a Pierce BCA Protein Assay kit as instructed by the manufacturer (Thermo Fisher; Waltham, MA).
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bioRxiv - Microbiology 2020Quote: ... Potential cytotoxicity of GaPPIX was determined by measuring the levels of released lactate dehydrogenase (LDH) in cell supernatants using an LDH Cytotoxicity Assay Kit (Invitrogen).
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bioRxiv - Microbiology 2019Quote: ... The quality of the DNA was assessed by gel electrophoresis and the quantity was estimated using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) and a Tecan Infinite 200 Microplate Reader (Tecan Deutschland GmbH).
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bioRxiv - Plant Biology 2021Quote: ... 5 mM MgCl2 and 0.5 mM MnCl2) and quantified by using Pierce™ BCA Protein Assay Kit (23227, Thermo scientific). In the case of protease digestions ...
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bioRxiv - Microbiology 2021Quote: ... the supernatant was collected and the protein concentration was determined with a BCA protein assay kit (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were used for HBsAg or luciferase measurement (Pierce Gaussia Luciferase Glow Assay Kit, ThermoFisher Scientific, Waltham, MA, USA). Cells were collected for HBV RNA transcript and cellular ribosomal RNA analysis by Northern blots.
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bioRxiv - Genomics 2020Quote: ... and the concentration of RNA samples was measured by Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA-depleted and purified RNA was used to construct the single-stranded circular DNA library with MGIEasy RNA Library preparation reagent set (MGI ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Genomics 2020Quote: ... We then amplified the library using Hi-fidelity KAPA PCR kit (KAPA) for 6 cycles and quantified the library using Qubit high sensitivity DNA assay (Thermofisher). We performed QC using bioanalyzer (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... OCR measurements were normalised to cell number which was quantified using the CyQUANT™ Cell Proliferation Assay Kit (ThermoFisher Scientific). Data was analysed using Wave software (Agilent).
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bioRxiv - Neuroscience 2020Quote: ... samples were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100 for 15 minutes and then stained for TUNEL following manufacturer’s instructions (Click-iT TUNEL assay kit from Life Technologies). Cells were blocked with 3% BSA for 1 hour and then incubated in primary and secondaries ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and normalized to total protein concentrations using the Pierce BCA Protein Assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration of clarified cell lysate was then measured using the BCA protein assay kit (Thermo Fisher, Cat# 23227). 5 ∼ 20 μg of total protein per lane was separated by SDS-PAGE gel and transferred to PVDF membrane ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of sc/snRNA-seq sequencing libraries was quantified by Qubit™ ssDNA Assay Kit (Thermo Fisher Scientific, #Q10212). The resulting libraries were sequenced using a DIPSEQ T1 or DIPSEQ T7 sequencers at the China National GeneBank (Shenzhen ...
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bioRxiv - Microbiology 2020Quote: ... with minor alterations including no vigorous mixing steps and final elution in 100μl of nuclease free water and quantified using a Qubit and the HS (high sensitivity) dsDNA assay kit (Thermofisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... checked on Agilent DNA1000 chip on Bioanalyzer2100 and quantified by Qubit Fluorometer 2.0 using Qubit dsDNA HS Assay kit (Life Technologies). After obtaining the Qubit concentration for the library and the mean peak size from Bioanalyzer profile ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Neuroscience 2021Quote: ... Protein from each well was extracted with 0.1% NP-40 PBS solution and quantified with BCA protein assay kit (Thermo Fisher), and data were normalized from protein concentration ...
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bioRxiv - Neuroscience 2020Quote: ... protein concentration in each sample was determined by using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). 75µg protein per condition was transferred into new microcentrifuge tubes and 5 µL of the 200 mM TCEP was added to reduce the cysteine residues and the samples were then incubated at 55°C for 1-h ...