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Citations for Thermo Fisher :
6851 - 6900 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Cell proliferation was assessed using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (ThermoFisher) as described above ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was obtained from cells isolated from organoid assays using the PicoPure RNA Isolation Kit (Cat#KIT0204; Applied Biosystems), following the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2019Quote: Cells were lyzed using RIPA buffer and proteins were quantified using the BCA method (Protein BCA Assay Kit, Thermo Fisher). The protein extracts were separeted on 12% SDS-PAGE gels (50 μg of total proteins were loaded per well ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration and quality of extracted RNA were evaluated using the Quant-iT RiboGreen RNA Assay Kit (Thermo Fisher Scientific) and the RNA 6000 Pico Chip (Agilent Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... Total concentration of protein in lysate was quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) measured at 562 nm and compared to a standard curve ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentrations of libraries were quantified using Qubit dsDNA HS Assay Kit and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, USA) and size validated using Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA integrity was determined by agarose electrophoresis and concentration measured using Qubit RNA Assay Kit in a Qubit 2.0 Fluorometer (Life Technologies, Invitrogen). cDNA libraries were synthetized from 1 µg of total RNA and sequenced using a HiSeq-3000 platform (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... EdU incorporation was also analyzed using Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Invitrogen, C10420) after 24h incubation with 10μM EdU ...
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bioRxiv - Developmental Biology 2020Quote: Cell cycle phase distribution analysis was conducted using Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Life Technologies) and FxCycle Violet (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... EdU incorporation assays were performed with the Click-iT® EdU Alexa Fluor® 647 Imaging Kit (C10339, Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... μl of DNA samples were normalized to a concentration of 10 ng/μl using Quant-iT Picogreen dsDNA Assay Kit (Invitrogen) for precision quantification ...
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bioRxiv - Microbiology 2022Quote: ... DNA extracted from seed samples was quantified with Qubit™dsDNA BR Assay Kit (ThermoFisher Scientific, Waltham, MA, United States) and verified with Polymerase Chain Reaction (PCR) ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants and lysates were assayed for LDH using an LDH colorimetric assay kit as per the manufacturer’s instructions (Invitrogen C20300). hMDMs were seeded at 500 000 cells per well in 12-well plates and differentiated as described above ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed once with PBS then lysed in lysis buffer provided in the LDH assay kit (Invitrogen C20300). Supernatants and lysates were assayed for LDH using an LDH colorimetric assay kit as per the manufacturer’s instructions (Invitrogen C20300) ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA concentration was measured using a Qubit DNA Assay Kit in a Qubit 3.0 Fluorometer (Life Technologies, CA, USA). DNA fragment size was tested using an Agilent Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentrations of the final purified samples were measured with Pierce™BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... DNA concentration of the purified eluate was quantified using a Qubit dsDNA BR assay kit (cat. no. Q32850, Thermo Fisher). Amounts of 500 ng purified tracer cDNA (insert ...
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bioRxiv - Molecular Biology 2021Quote: ... and retrieved DNA as well as input DNA was quantified using Qubit dsDNA High Sensitivity assay kit (#Q32854, Life Technologies). The concentration of ChIP-DNA and input samples were adjusted to maintain a similar ratio of ChIP-DNA:INPUT between different ChIP experiments ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration was measured using Qubit® DNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, Carlsbad, CA, USA). An amount of 100 ng genomic DNA (for whole genome sequencing - WGS ...
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bioRxiv - Genomics 2020Quote: ... EdU detection was performed using the Click-IT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Life Technologies, C10634). Propidium (PI ...
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bioRxiv - Immunology 2021Quote: ... for assessment of edU incorporation we used the Alexa Fluor 647-azide using Click-iT Plus EdU Flow Cytometry Assay Kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Samples from all methods were quantified after trypsin digest using a Micro BCA protein assay kit (Thermo Fisher Scientific #23235) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and CCL21 levels were normalized to total protein concentration of the homogenates determined using Pierce BCA protein Assay kit (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... Purity was determined by SDS-PAGE and protein concentrations were determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). For generation of POM1 mutants ...
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bioRxiv - Molecular Biology 2019Quote: ... The total RNA concentration was measured using a Qubit 1.0 Fluorometer and Qubit RNA BR Assay Kit (Thermo Fisher Scientific), and RNA quality and integrity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: DNA damage to HSPCs was observed using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling (TUNEL) immunostaining (Click-iT TUNEL Alexa Fluor Assay Kit, ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... and genomic DNA was quantified using a Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA). For GBS ...
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bioRxiv - Microbiology 2019Quote: ... according to the manufacturer’s instructions and quantified by Quant-iT dsDNA HS assay kit (Invitrogen molecular probes-Eugene, Oregon, USA). Microbial diversity was screened by Denaturing Gel Gradient Electrophoresis (DGGE) ...
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bioRxiv - Microbiology 2019Quote: Fluorescence labeling of proliferating cells were achieved by using an EdU cell proliferation assay kit (C10638, Thermo Fisher Scientific, US). A coral fragment was placed in a sealed chamber filled with 50–100 times volume of FSW containing 20 μM EdU ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA quality and concentration were determined by denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... Isolation of bacterial genomic DNA was performed using Sigma GenElute™ Bacterial Genomic DNA Kit and quantified using Qubit dsDNA BR assay in a Qubit 2.0 fluorometer (Invitrogen). 200 ng of genomic DNA was fragmented in a Covaris M220 focused-ultrasonicator (peak power ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The concentration of the RNA was determined by Qubit fluorometric quantitation (Qubit RNA BR Assay Kit, ThermoFisher Scientific, catalog # Q10210). The integrity of the total RNA was determined by a Fragment Analyzer (Advanced Analytical Technologies ...
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bioRxiv - Genomics 2019Quote: ... Genomic DNA was quantified with a Qubit 2.0 fluorometer using the Qubit dsDNA BR assay kit (Life Technologies, Carlsbad, CA) and diluted in buffer EB to the 20 ng/ul input for library preparation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The amount of protein in each sample was determined using a BCA protein assay Kit (Thermo Scientific, Waltham, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: Proteins were isolated using RIPA buffer and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Total RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: 1,000 cells were plated in 96-well culture plates and cell proliferation was determined by CyQUANT cell proliferation assay kit (C35011, Invitrogen) as described by the manufacturer.
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bioRxiv - Cancer Biology 2019Quote: ... The concentration of individual libraries was quantified by fluorescence using the Qubit dsDNA high sensitivity assay kit (Thermo Fisher Scientific). Individual indexed libraries were mixed in equimolar ratio and were further purified using a QIAquick PCR purification kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... The concentration was determined using a Qubit double-stranded DNA HS assay kit and a Qubit 2.0 fluorometer (Thermo Scientific), according to manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... The Click-IT Plus TUNEL Assay for In Situ Apoptosis Detection Alexa Fluor™ 488 dye kit (ThermoFisher Scientific, #C10617) was used ...
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bioRxiv - Genetics 2019Quote: ... The concentration of each cell was measured using the Qubit dsDNA High Sensitivity Assay Kit (Life Technologies/Thermo Fisher Scientific). Expected concentration of the single cell lysates was 20-60 ng/μL with size distribution of 300-2000 bp.
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bioRxiv - Genetics 2019Quote: ... The concentration of each cell was measured using the Qubit dsDNA High Sensitivity Assay Kit (Life Technologies/Thermo Fisher Scientific). Expected concentration of the single cell lysates was 20-60 ng/μL with size distribution of 300-2000 bp.
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bioRxiv - Genetics 2019Quote: ... The concentration of each cell was measured using the Qubit dsDNA High Sensitivity Assay Kit (Life Technologies/Thermo Fisher Scientific). Expected concentration of the single cell lysates was 20-50 ng/μL with size distribution of 300-1000 bp.
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bioRxiv - Genetics 2019Quote: ... The concentration of each cell was measured using the Qubit dsDNA High Sensitivity Assay Kit (Life Technologies/Thermo Fisher Scientific). Expected concentration of the single cell lysates was 20-50 ng/μL with size distribution of 300-1000 bp.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Protein was reconstituted from the matrices after desiccation overnight and recovery was quantified using EnzChek Lysozyme Assay Kit (ThermoFisher Scientific). Fluorescence measurements were performed in triplicates for each sample and protein recovery calculated by interpolation on a standard curve ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA).
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bioRxiv - Genomics 2021Quote: ... DNA was quantified with the dsDNA High Sensitivity (HS) Assay Kit on the Qubit 2.0 or Qubit 3.0 Fluorometer (Thermo Fisher Scientific) to determine input into NGS library preparation.
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bioRxiv - Developmental Biology 2020Quote: ... RNA concentration was then quantified via Qubit Fluorometer using the RNA BR Assay Kit (Qiagen, ThermoFisher Scientific, cat no: Q10210,) and quality was evaluated using an Agilent 2100 BioAnalyzer (Agilent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was quantified using first a NanoDrop ND8000 spectrophotometer and then a Qubit 2.0 fluorometer with the DNA HS assay kit (Life Technologies). DNA quality was examined on agarose gels ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing proteins was collected and the protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Scientific). Immunoblotting analysis was carried out as previously described.(8 ...
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bioRxiv - Genomics 2021Quote: ... the RNA concentration was quantified using the Qubit RNA HS Assay Kit on a Qubit Fluorometer (Life Technologies, Carlsbad, California). RNA quality was determined via the RNA Pico Sensitivity Assay on a LabChip GXII Touch (PerkinElmer ...