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Citations for Thermo Fisher :
6751 - 6800 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The protein concentration of supernatant lysates was determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific) and a NanoDrop ND1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentrations of solubilized protein precipitates were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Bremen, Germany). For LC-MS/MS ...
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bioRxiv - Developmental Biology 2023Quote: ... The cleavage detection assay was done as described by Stolfi et al.52 (GeneArt Genomic Cleavage Detection Kit, Thermofisher). The efficiency of CRISPR/Cas9 editing was ≥39.0% across multiple independent experiments ...
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bioRxiv - Cell Biology 2022Quote: ... The total protein concentration was determined using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher #53226). 30 μg of total protein sample was mixed with Laemmli buffer (180 mM DTT (Sigma #D9779) ...
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bioRxiv - Biochemistry 2023Quote: ... the DNA was quantified using the Qubit 1X dsDNA HS assay kit and a Qubit 4 Fluorometer (both Invitrogen), and the DNA of the insertion libraries from the same assay was pooled in an equimolar ratio ...
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bioRxiv - Bioengineering 2023Quote: The proliferation of SCPC and hiPSC was measured with the Click-iT EdU flow cytometry assay kit (Invitrogen, USA). EdU (5-ethynyl-2’-deoxyuridine ...
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bioRxiv - Cancer Biology 2022Quote: ... The EV and cell lysate protein concentrations were determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The protein lysates were separated using Bolt 4–12% Bis-Tris Plus Gels (Burlington ...
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bioRxiv - Genomics 2023Quote: ... and DNA quantification was performed with Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo Fisher Scientific, Massachusetts, USA) using a Synergy HT plate reader (BioTek ...
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bioRxiv - Neuroscience 2023Quote: Proteolytic activity of MMPs was measured in brain slices using EnzChek Gelatinase/Collagenase Assay Kit (E-12055, ThermoFisher Scientific). This kit contains fluorogenic DQ gelatin (highly quenched fluorescein-labeled gelatin ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations of the EV isolates were determined using the PierceTM BCA protein assay kit (Thermo Fisher Scientific) and 10 ug aliquots were prepared and stored at -80°C until further analysis.
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bioRxiv - Plant Biology 2023Quote: ... DNA concentration was measured using Qubit® DNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). A total amount of 1.5µg DNA per sample was used as input material for the DNA sample preparations ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration of the supernatant was then determined using Pierce™ BCA Protein Assay Kit (# PI23225, Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Protein concentrations of all samples were determined using the Pierce Bicinchoninic Acid (BCA) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Results were normalized by total protein concentrations as determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, catalog #23225). PBMC lysates were not diluted for this assay ...
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bioRxiv - Cell Biology 2023Quote: ... Protein extracts were centrifuged at 14,000 x g at 4°C for 20 minutes and quantified with Pierce BCA Protein Assay Kit (ThermoFisher). 10 μg or 20 μg of proteins were separated by electrophoresis in denaturing SDS polyacrylamide gel (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified by performing a 1.8X AMPure XP bead cleanup and quantified using the Qubit dsDNA HS assay kit (Q32851, Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... Quantitation of Total RNA was performed with the Qubit BR RNA Assay kit and Qubit Flex Fluorometer (Invitrogen/ThermoFisher), and quality assessment performed by RNA ScreenTape analysis on an Agilent TapeStation 2200 ...
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bioRxiv - Plant Biology 2023Quote: ... Total protein content in the supernatant was estimated using the Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL). The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The total RNA concentration in each lysate was measured using a Qubit RNA BR Assay Kit (Thermo Fisher Scientific). Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen ...
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bioRxiv - Microbiology 2023Quote: ... following the manufacturer’s protocol (Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit; ThermoFisher Scientific, Rockford, IL, USA). From each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein content in the supernatant was determined using a colorimetric micro–BCA Protein Assay kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... and normalized to protein levels determined in the same lysate by Pierce BCA Protein Assay Kit (Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2023Quote: ... gDNA was diluted to 0.5 ng/μL (as quantified by Quant-iT dsDNA BR assay kit and a Qubit fluorometer (Invitrogen). Ten-point standard curves were prepared from two-fold serial dilutions of this 0.5 ng/μL starting point ...
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bioRxiv - Molecular Biology 2023Quote: ... The yield and quality of sequencing libraries were assessed by Qubit HS dsDNA Quantification Assay Kit (Thermo Fisher Scientific) and TapeStation 4150 System (Agilent) ...
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bioRxiv - Biophysics 2023Quote: ... then the pooled amplicon libraries were gel extracted and quantified using the QuantIt dsDNA assay kit (Invitrogen ref Q33130) or via qPCR at the Vincent J ...
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bioRxiv - Bioengineering 2023Quote: ... The encapsulation efficiency (EE) of mRNA was measured using the Quant-it Ribogreen Assay Kit (Thermo Fisher Scientific, USA). The pKa of LNP was determined using the TNS binding assay14.
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bioRxiv - Immunology 2023Quote: ... Low input RNA-Seq: total RNA was quantified with Qubit RNA HS Assay Kit (Thermo Fisher Scientific catalog#: Q32852) and quality was assessed using RNA ScreenTape on 4200 TapeStation (Agilent Technologies catalog# ...
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bioRxiv - Systems Biology 2023Quote: ... Protein concentrations in the pellet were determined using a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, MA). After mixing 400 μL of supernatant with 400 μL of chloroform and 320 μL of water ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentration was quantified using a Qubit fluorometer and 1X dsDNA High Sensitivity (HS) assay kit (ThermoFisher Scientific, USA).
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bioRxiv - Pathology 2023Quote: ... Total RNA from Mus musculus was quantified by Qubit® RNA BR Assay kit (Thermo Fisher Scientific, Bremen, Germany) and the RNA integrity was estimated by using RNA 6000 Nano Bioanalyzer 2100 Assay (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the extract was determined using a BCA protein assay kit (Thermo Fisher Scientific, cat. no. 23225) and adjusted to 2 μg/μL using the IP lysis buffer.
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bioRxiv - Microbiology 2023Quote: ... The purified PCR products were then quantified using the Quant-iT High-Sensitivity dsDNA Assay Kit (Thermo Fisher Scientific) in a Tecan Spark® Multimode Microplate reader (Tecan) ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% [v/v] 2-mercaptoethanol) and then quantified with the Pierce BCA protein assay kit (Thermo Fisher Scientific). The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... Protein content of lysates was quantified using the PierceTM Protein Assay Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). 20 µg of the protein samples were then heat-denatured and subsequently separated on a 4-12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was isolated by hot phenol-chloroform extraction and quantified using Qubit RNA High Sensitivity Assay Kit (Invitrogen). 1.5 μg of total RNA was used for rRNA depletion by binding of complementary oligos and treatment with RNase H87,88 ...
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bioRxiv - Plant Biology 2024Quote: ... RNA concentrations were measured using a Qubit RNA Assay Kit on a Qubit 2.0 Fluorometer (Life Technologies, CA, USA). RNA purity was checked using a Nanophotometer spectrophotometer (IMPLEN ...
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bioRxiv - Microbiology 2024Quote: ... and concentrations of indexed cDNA were measured using the Qubit®dsDNA BR Assay Kit (Invitrogen, Carlsbad, CA, USA). Approximately 28 ng of DNA from each sample was added to a pool ...
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bioRxiv - Molecular Biology 2024Quote: ... and the RNA concentration was measured using a Qubit Fluorometer with the Qubit RNA High Sensitivity Assay Kit (Invitrogen). The quality of the RNA was assessed using a high sensitivity electrophoresis system ...
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bioRxiv - Microbiology 2024Quote: ... and residual DNA concentrations were measured with the Qubit® dsDNA BR Assay Kit (Life Technologies, Leusden, the Netherlands). Samples which contained over 10 ng/µL DNA contamination were treated with the Turbo DNAfree® Kit (Ambion ...
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bioRxiv - Microbiology 2024Quote: ... quantified using the QubitTM dsDNA BR Assay Kit (Q32850) and a Qubit spectrofluorometer (Qubit 3.0, Thermo Fisher Scientific, USA), and submitted for complete sequencing (Plasmidsaurus Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and measured for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2024Quote: ... The concentration of RNA was estimated using a Qubit 3.0 (Cat no: Q33238, RNA HS Assay Kit; Cat no:Q32855, ThermoFisher Scientific), and the quality of RNA based on the RNA Integrity Number was estimated using either the 2100 BioAnalyzer (Cat no ...
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bioRxiv - Cell Biology 2024Quote: ... OCR measurements were normalized to cell number as determined by the CyQUANT Cell Proliferation Assay Kit (Thermo Scientific, C7026) or DAPI staining for HeLa and AML12 cells ...
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bioRxiv - Immunology 2024Quote: ... Quantification of cell free dsDNA performed by fluorometry using PicoGreen assay following the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Kit; Invitrogen). Plates were read on GloMax Multi Detection System (Promega ...
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bioRxiv - Microbiology 2024Quote: ... The quality and quantity of the extracted DNA was analyzed with a Qubit 4.0 Fluorometer using the dsDNA high-sensitivity assay kit (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... JC-1 assay kit (Cat. No. M34152) and Dulbecco’s modified eagle medium (DMEM) (Cat. No. 11965084) were purchased from ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 6000 rpm 4°C and protein concentration was estimated by BCA protein assay kits (Thermo-Fisher Scientific, Cat #23225).
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bioRxiv - Bioengineering 2024Quote: ... SYBR Green I nucleic acid gel stain (10,000X concentrate in DMSO) (S7563) and Qubit dsDNA HS Assay Kit (Q32851) were purchased from Invitrogen. UltraPure TBE buffer (10X) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by the quantification of qualified RNA by Qubit3.0 with QubitTM RNA Broad Range Assay kit (Life Technologies, Q10210). A total of 2 micrograms of RNA were used for building a stranded RNA sequencing library using the KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® (DR08502 ...
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bioRxiv - Cancer Biology 2024Quote: ... The total protein concentration of each cell lysate was determined with a BCA protein assay kit (Thermo Fisher, # 23225). For each sample ...