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Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Qualified RNAs were finally quantified by Qubit3.0 with Qubit™ RNA Broad Range Assay kit (Life Technologies, Carlsbad, CA, USA). Total RNAs (2 μg ...
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bioRxiv - Immunology 2021Quote: Total protein concentrations in BAL fluid were detected by Pierce BCA Protein Assay Kit according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was measured using Pierce BCA Protein Assay kit according to manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). 20 µg of total protein/lane were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... Click reaction was carried out with Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay kit (Thermo Fisher C10456) according to the manufacturer’s directions.
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bioRxiv - Bioengineering 2021Quote: ... including nickel-nitrilotriacetic acid (Ni-NTA) agarose and Pierce Rapid Gold BCA Protein Assay Kit were purchased from Fisher Scientific International Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... The amount of RBD released at each timepoint was determined using a Micro BCA™ Protein Assay Kit (Fisher Scientific) following the manufacturer’s instructions (including using the Bovine Serum Albumin standards provided in the kit) ...
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bioRxiv - Bioengineering 2021Quote: ... and stored at −80 °C to be quantified by bicinchoninic acid (BCA) protein assay kit [75] according to manufacturer’s instructions using a bovine serum albumin standard curve (ThermoFisher Scientific). The pellet was discarded.
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bioRxiv - Bioengineering 2020Quote: ... a live/dead assay was performed on CCL-149 in lung ECM hydrogels using a commercially available kit (Thermo Fisher). A Zeiss confocal microscope L710 was used to visualize cells in lung ECM hydrogels ...
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bioRxiv - Bioengineering 2020Quote: ... DNA content in the tissues was quantified using the Quant-iT™ PicoGreen® dsDNA assay kit (Thermo Fisher Scientific) as per the manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: The supernatants were collected after cytotoxicity assay for cytokine analysis by using IFN-γ and IL-2 ELISA kits (Invitrogen). Cytokine levels were analyzed by comparing the values obtained from samples to a standard curve generated from known concentrations of the cytokines.
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bioRxiv - Biochemistry 2021Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were adjusted in 5xLDS sample buffer containing 50 mM DTT ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations of membrane fractions in collected supernatant samples were measured by BCA protein assay kit (Thermo Fisher Scientific 23225).
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bioRxiv - Molecular Biology 2020Quote: ... EdU staining was performed using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen, CatNo C10634) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... according to the manufacturer’s instructions and quantified using Qubit RNA BR Assay kit on Qubit 2.0 Fluorometer (Thermo Fisher Scientific). One microgram of total RNA was reverse transcribed using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Cell cycle analysis was performed using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific) and FxCycle Violet Stain (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein concentration of the supernatants was determined using Pierce 660 nm Protein Assay Reagent Kit (22660, Thermo Fisher Scientific) supplemented with Ionic Detergent Compatibility Reagent (22663 ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was quantified using Qubit™ RNA HS Assay Kit and Qubit® Fluorometer (Thermo Fisher Scientific, MA, USA). RNA fragment size was analyzed with the 2100 Bioanalyzer (Agilent ...
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bioRxiv - Physiology 2020Quote: ... An aliquot (10%) was removed for quantification using the Pierce Quantitative Fluorometric Peptide Assay kit (Thermo Scientific, Cat. No. 23290). The remainder of the peptides from each IP samples (∼0.5-3.5 µg ...
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bioRxiv - Plant Biology 2021Quote: ... cells were treated with reagents of an Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Molecular Probes/Invitrogen, Carlsbad, CA, USA), as has been used by previous researchers (Lin et al. ...
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bioRxiv - Plant Biology 2021Quote: ... The clear supernatant was used for measuring PPi concentration by using PiPer Pyrophosphate Assay Kit (Life Technologies, Carlsbad, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and then quantified on the Qubit 3.0 fluorometer using the DNA high sensitivity assay kit (Thermo-Fisher Scientific). The consortium metagenomic library was prepared with 200 ng of starting material using the TruSeq DNA Nano kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: The resulting libraries’ concentrations and size distributions were assessed on a Qubit 3.0 fluorometer using the DNA high sensitivity assay kit (Thermo-Fisher Scientific) and a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Microbiology 2021Quote: The Gaussia luciferase activities were measured using a luminometer using the Pierce Gaussia luciferase flash assay kit (Thermo Scientific, 16158).
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bioRxiv - Microbiology 2021Quote: ... DNA was quantified using the Qubit dsDNA High Sensitivity assay kit on a Qubit 2.0 fluorometer (Thermo Fisher Scientific, Canada). The V3 and V4 hypervariable regions of the 16S rRNA gene were amplified in triplicate PCR reactions per extraction using the primer pair SD-Bact-341-bS17/SD-Bact-785-aA2146 modified with Illumina MiSeq overhang adapters ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration of the elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) and the elution fraction with the highest protein concentration was chosen for further use.
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bioRxiv - Microbiology 2021Quote: ... the extracted DNA samples were checked for quality and quantity by a 1% agarose gel (Midori Green-stained) electrophoresis and a Qubit dsDNA HS Assay Kit (Invitrogen). Prior to library preparation ...
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bioRxiv - Microbiology 2021Quote: ... using a Perkin Elmer Victor X3 multimode plate reader and an EnzChek™ Lysozyme Assay Kit (E-22013) from ThermoFisher Scientific at a temperature of 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Final PCR products were gel purified and individual DNA concentrations were determined with the Qubit dsDNA High Sensitivity Assay Kit on the Qubit Fluorometer (Invitrogen). Samples were quality controlled using the Bioanalyzer High Sensitivity DNA Analysis Kit and the Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: Total protein levels were quantified in tissue homogenates using the Pierce Microplate BCA Protein Assay Kit in triplicates following the manufacturer’s protocol (Thermo Scientific Pierce Protein Biology ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was quantified using fluorescent chemistry contained in the Qubit RNA HS Assay Kit (Cat# Q10211, Thermo Fisher Scientific). 1 ul was used to assess RNA Integrity Number (RIN ...
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bioRxiv - Physiology 2021Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and DNA concentration was calculated by comparison with Quant-iT standards (Invitrogen Qubit dsDNA HS Assay Kit, Thermo Fisher Scientific) in liquid Tryptone NaCl medium.
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bioRxiv - Microbiology 2021Quote: ... The gDNA was quantified using a Qubit™ fluorimeter and the Quant-iT™ dsDNA BR Assay Kit (Invitrogen, USA), and the quality was assessed with a NanoDrop One instrument (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were stained using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific – C10420), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... cells were treated with reagents of an Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Molecular Probes/Invitrogen, Carlsbad, CA, USA), as has been used by previous researchers (Lin et al. ...
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bioRxiv - Microbiology 2021Quote: ... Concentration of the DNA was determined with a Qubit fluorometer by using the Qubit HS dsDNA Assay kit (Molecular Probes).
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Cell-free DNA as a biomarker for prostate cancer: elevated concentration and decreased fragment sizebioRxiv - Cancer Biology 2020Quote: Extracted DNA was quantified with a Qubit 3.0 Fluorometer and a DNA dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA), as well as on the 2100 Bioanalyzer with High Sensitivity DNA Chips (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein content of all extracts was determined using the Pierce BCA Protein Assay Kit (Cat no. 23225, ThermoFisher Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: Viability of KTB21 cells on the matrices was determined using the Live/dead cell viability assay kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Rho pull-down assays were performed using GST-rhotekin and Rho-pull down detection kit according to manufacturer’s instructions (Thermo Scientific). The levels of total and active Rho were established by using a Rho-specific antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... phosphatase inhibitors) and proteins concentrations were measured using a Pierce BCA protein Assay Kit (Thermo Fisher Scientific, Ref: 23225/23227). 10 μg of proteins were then separated on a 4-15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the protein was centrifuged at 10,000×g for 10 min and quantified by BCA Protein Assay Kit (Thermo Fisher Scientific). The same quantity (about 15 μg ...
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bioRxiv - Cell Biology 2021Quote: Total cell lysates were collected using RIPA buffer and quantified with the BCA Protein Assay kit (Thermo Fisher Cat#: 23227). Samples were mixed with LDS sample buffer (Invitrogen Cat# ...
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bioRxiv - Cell Biology 2021Quote: ... and lactate and pyruvate were measured using colorimetric assays using kits per manufacturer’s instructions (insulin, Cat no. RI-13K, Millipore Sigma, Burlington, MA; lactate, Cat no. A22189, Invitrogen, Carlsbad ...
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bioRxiv - Cell Biology 2021Quote: ... the measurement of combined nitrite and nitrate levels were used as the amount of NO bioavailability using the Nitric Oxide Assay kit (cat# EMSNO, ThermoFisher).
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bioRxiv - Neuroscience 2020Quote: ... Total DNA was measured in all extracts by using the Qubit dsDNA HS Assay kit (Thermo Fisher Scientific, Karlsruhe, Germany).
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bioRxiv - Cell Biology 2020Quote: ... EdU was pulsed in cells for 24h before fixation and stained according to the manufacturer’s instructions (Click-iT EdU assay kit, Life Technologies).
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bioRxiv - Plant Biology 2020Quote: ... Total RNA quality was assessed by denaturing agarose gel electrophoresis and quantified by the Qubit RNA BR Assay Kit (ThermoFisher). For library preparation ...
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bioRxiv - Genomics 2020Quote: ... Total protein amount was measured from 10 µL of the sample using the Pierce BCA protein assay kit (Thermo Fisher). The levels of radioactive methionine were measured by scintillation counting with 10-80 µL of the resuspended protein ...