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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Protein extraction was performed as described previously37 and protein concentration was determined using Pierce BCA protein assay kit (Thermo scientific).
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bioRxiv - Molecular Biology 2022Quote: ... we performed RNA-Protein pull-down assay by using Pierce™ Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific). The biotin labeled RP24-315D19.10 and AS-RP24-315D19.10 probe was designed and synthesized by GenePharma company ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were checked for correct fragment length on an Agilent 2100 Bioanalyzer and pooled equimolarly and quantified with Qubit DNA HS Assay Kit (ThermoFisher). Equimolarly pooled libraries were sequenced on a MiSeq Reagent Kit v2 (300 cycles ...
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bioRxiv - Systems Biology 2024Quote: ... The double-stranded cDNA was eluted into 15 µl nuclease-free water and concentration measured using a Qubit Fluorometer 3.0 with dsDNA high-sensitivity assay kit (ThermoFisher Q32851).
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bioRxiv - Neuroscience 2023Quote: ... and cDNA was quantified using a Qubit 1xdsDNA HS assay kit (cat#: Q33231) on a QuBit 4.0 fluorometer (Invitrogen, ThermoFisher Scientific). Libraries with unique indices were then pooled in equimolar ratios before sequencing.
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bioRxiv - Neuroscience 2024Quote: ... Quantification of the library was performed using dsDNA HS assay kit and Qubit (Molecular Probes, Life Technologies, Rhenium, Modi’in, Israel) and quantification was performed using the Agilent DNA HS kit and Bioanalyzer 2100 ...
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bioRxiv - Physiology 2023Quote: ... and processed for Click-iT EdU detection according to manufacturer’s protocol (Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit; Thermo Fisher). All samples were analyzed and sorted using an Aria Fusion (Becton Dickinson ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample protein concentration was measured using the Micro BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA; Cat.: 23235). 20 μg of total protein lysate was resolved by electrophoresis on Bis-Tris 4-12% NuPAGE polyacrylamide gels using the Novex Mini Cell system (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The DNA concentration was measured using a Qubit fluorometer and a dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA), and purity was determined by measuring the A260/A280 absorbance ratio with a NanoDrop spectrophotometer (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Following double-sided size selection by bead-purification the libraries were quantified with Qubit dsDNA HS assay kit (ThermoFisher #Q32851) and pooled for sequencing on NovaSeq6000 (SP 2 x 100bp).
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bioRxiv - Physiology 2024Quote: ... Supernatant (cleared lysates) were used to determine total protein amount using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s instructions and quantified using the Qubit Fluorometer (dsDNA HS assay kit, Life Technologies, Carlsbad, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... according to the manufacturer’s instructions and quantified with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... We determined the changing of cholesterol level on the cell membrane using Amplex™ Red Cholesterol Assay Kit (Thermo Scientific) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Lysates were centrifuged at 14000 rpm for 15 min with the soluble fraction quantified using the Micro BCA Protein Assay Kit (Thermofisher). 500µg protein lysate was volume normalized with NP40 buffer (inhibitor cocktail supplemented ...
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bioRxiv - Microbiology 2024Quote: ... and 25 µl of the supernatant was used to determine the protein amount following the manufacturer’s instructions using a Pierce BCA Protein Assay Kit (Cat#23225, Thermo Scientific). A vial containing a quality control mixture pool of 12.5 µl from each sample (150 µl total ...
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bioRxiv - Neuroscience 2024Quote: Protein concentrations of each sample were determined using the bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek ...
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bioRxiv - Neuroscience 2024Quote: ... the EdU detection was performed using the Alexa Fluor-647 Click-iT EdU Cell Proliferation Assay kit (Invitrogen Cat# C10424), following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... the supernatant containing the protein extract was collected and the total protein concentration was determined (BCA Protein Assay Kit, Thermofisher). Samples were reduced with DTT (15 mM ...
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bioRxiv - Genomics 2024Quote: ... Protein concentration was measured with Pierce™ BCA Protein Assay Kit according to manufacturer’s guidelines (Thermo Fisher; Cat. No. 23225). Protein samples were mixed with 4X NuPAGE™ LDS Sample Buffer (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of the resulting protein pellet was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Cat. 23225) and a NanoDrop™ 2000/2000c spectrophotometer (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... PCRs were run using between 10-500 pg of DNA measured by Quant-iT Qubit dsDNA HS Assay Kit (ThermoFisher) to find the amount of amplifiable alleles for each sample ...
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bioRxiv - Microbiology 2023Quote: ... All aliquots from each donor were combined and quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher, Massachusetts, USA).
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bioRxiv - Microbiology 2024Quote: ... DNA concentration was measured using the Qubit® dsDNA Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). The DNA quality control ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell cycle analysis was performed using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen #C10634). Cells were incubated for 2 hours with 10uM EdU and stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Invitrogen #L34955 ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein pellets were resuspended in 1% SDS and protein concentration was measured using the Bradford assay kit (Thermo Scientific, 1863028), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: Levels of proliferation in hiPSC-RPE monolayers were assessed using a proliferation assay kit Click-iT EdU (Thermo Fisher, C10337). Cells were incubated with a 1:1 mixture of cell culture supernatant and 5-Ethinyl-2’-Desoxyuridin (EdU ...
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bioRxiv - Microbiology 2024Quote: ... The final purified DNA was stored at -80 °C and the DNA concentration was determined using Qubit HS Assay Kit (Invitrogen) on the Qubit 4 Fluorometric Quantification device (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: ... The protein concentration in the MFS was quantified with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Cat. # 23225), using BSA as a standard ...
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bioRxiv - Genomics 2024Quote: ... Products were visualized via electrophoresis on an Agilent TapeStation 4200 and quantified with Qubit dsDNA HS assay kit (Invitrogen, Q32851) on a Qubit fluorometer (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The amplicon DNA concentration was measured using Qubit dsDNA HS and BR Assay Kits (Thermo Fisher Scientific, Waltham, MA, USA). Library quality was determined using the Agilent Technologies 2100 Bioanalyzer system with a DNA-1000 chip ...
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bioRxiv - Microbiology 2024Quote: ... The DNA concentration was measured using a Qubit fluorometer and a dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA), and purity was determined by measuring the A260/A280 absorbance ratio with a NanoDrop spectrophotometer (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... mRNA detection (FISH) in sorted T cells was carried out using the ViewRNA Cell Plus Assay kit (Cat. No. 88-19000, Invitrogen). The slides were imaged using a Zeiss LSM800 confocal microscope and analyzed with ZEN software (Carl Zeiss ...
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bioRxiv - Immunology 2024Quote: ... The concentration of the oligo dT primer on the agarose was quantified using the Qubit ssDNA assay kit (Invitrogen, #Q10212) and normalized to 8 µM.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Quantification of cleaned libraries were measured using a Quant-iTTM PicogreenTM dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Germany). Based on the concentration ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Total protein concentration of the cleared lysate was assessed with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). IL-1β was quantified from hippocampi tissue lysates with V-PLEX Mouse IL-1β Kit (Meso Scale Discovery ...
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bioRxiv - Cell Biology 2023Quote: ... The quantity and quality of the purified total RNA were assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR or HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The purified total RNA was assessed for quantity and quality using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR or HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ BCA Protein Assay Kit and Halt™ Protease and Phosphatase Inhibitor Single-Use Cocktail were from Thermo Scientific™ ...
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bioRxiv - Bioengineering 2024Quote: Chondrocyte ROS production was measured using the CellROX Green Flow Cytometry Assay Kit according to the manufacturer’s instructions (Invitrogen, C10492). After 24 hrs of +/-t-BHP stimulation ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce™ Quantitative Peptide Assays kit (Thermo Fisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip as previously (38) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined in a 96-well format using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... The protein content of the resulting lysate was quantified using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA) according to manufacturer instructions ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... and the resulting supernatants were collected and assayed for protein concentration with a BCA Protein Assay Kit (Thermo Fisher, 23225). Samples containing equal amounts of protein were denatured by boiling for 10 min with Laemmli sample buffer and then fractionated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... we conducted cell-cycle analysis using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific). Cells seeded at a density of 5 × 105 cells per well in a 6-well plate were cultured for five days in StemFit AK02N media supplemented with Dox ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate was cleared by centrifugation at 11,000 g for 5 min and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: ... EdU incorporation was determined with the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Invitrogen, C10419) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: Protein concentrations of samples (fractions 6-9) were determined with a Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, USA), and Bradford assay (Biorad ...
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bioRxiv - Cancer Biology 2024Quote: ... the Live/Dead assay was conducted using 1 μM calcein AM and 4 μM ethidium homodimer-1 (Life Technologies kit) for 30 minutes at 37 °C ...