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Citations for Thermo Fisher :
7051 - 7100 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and analyzed genes were amplified using 4x Capital qPCR Green Master mix (BiotechRabbit - BR0501702) on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific). For Xbp1 spliced and total quantification were performed in six different biological replicates ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was carried out using TaqMan gene expression master mix (Applied Biosystmes) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). All primers and probes information for each target gene were obtained from Custom TaqMan Probes (https://www.thermofisher.com/order/custom-oligo/custom-taqman-probes).
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bioRxiv - Neuroscience 2024Quote: ... and PowerTrack™□ SYBR™□ Green Master Mix in StepOne Plus Real-Time PCR 96-well system (Thermo Fisher Scientific Inc., USA). The sequences of primers (forward and reverse ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCRs were performed using PerfeCTa SYBR green Fastmix (Quantabio, Cat. #101414-276) on a StepOnePlus™ real-time PCR system (Thermo Fisher). 18S or β-actin were used as the internal loading control for qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was isolated by phenol-chloroform extraction followed by an ethanol precipitation and analyzed by qPCR using StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) and SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR was carried out to determine the expression levels of selected genes using a StepOne plus real-time PCR system (Thermo Fisher Scientific). The primer details were listed in Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... was used to amplify specific circRNAs using its divergent primer pairs in QuantStudio 3/6 Real-Time PCR System (Thermo Fisher Scientific). The delta-CT method was used to check for the relative abundance of individual circular RNA molecules ...
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bioRxiv - Molecular Biology 2024Quote: ... and analyzed with PerfeCa® SYBR® Green FAstMix® ROX (Quanta bio) in the StepOnePlusTM Real-time PCR System (Applied Biosystems) using specific primers designed for each targeted gene (Supplemental Table 3) ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR measurements were performed in an ABI 7300 Real-time PCR machine using Maxima™ SYBR Green/ROX qPCR Master Mix (Thermo Scientific). Primer sequences used in this study can be found in Supplementary Table 3 ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR-based gene expression analysis was conducted using the Taqman Fast Advanced Master Mix in the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) using the following Taqman probes (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using a PowerUp SYBR Green Master Mix and standard protocols on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific). RPL19 was used to normalize RNA isolated from mouse samples ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Real-time PCR was performed to measure the relative mRNA levels using the QuantStudio™ 5 Real-Time PCR System with SYBR Green Master Mix (Applied Biosystems). The primer sequences are described in supplementary table 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time PCR was performed in 20 µl reactions using 10 µl TaqMan™ Fast Advanced Master Mix (Applied Biosystems, Carlsbad, CA), 0.25 µl forward primer (40 µM) ...
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bioRxiv - Microbiology 2024Quote: ... Japan) and the intensity of the fluorescent signal was detected using a 7500fast real-time PCR system (Applied Biosystems, Foster, CA, USA). The primers of selected genes are shown in Table S1 ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was carried out using a PCR system (StepOne; Applied Biosystems) and the Luna One-Step RT-qPCR Kit (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CD33m was amplified by PCR using SuperScript III One-Step RT-PCR (Invitrogen) and Gene-specific primers 5’-TTATATGCTAGCGCCACCATGCCGCTGCTGCTACTGCTGC-3’ ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-qPCR PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems) using primers listed in Table 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... RT–PCR was done using SYBR Green PCR Master Mix (Applied Biosystems, 4309155) on a ViiA 7 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed on StepOne® Realtime PCR system (Applied Biosystems) using KAPA SYBR FAST qPCR Kits (Roche ...
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bioRxiv - Immunology 2022Quote: ... followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems) in QuantStudio3 (Applied Bioscience) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed using Dream-Taq Green PCR master mix (Thermo Fisher). The RT-PCR was performed to validate the expression of full-length Cas12f and tracrRNA-crRNA in the plant ...
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bioRxiv - Microbiology 2021Quote: ... The presence of SARS-CoV-2 RNA was determined by conventional reverse transcription PCR using the SuperScript®III One-Step RT-PCR System with Platinum® Taq DNA Polymerase kit (Invitrogen) in a 25μl reaction volume containing 12.5μl of 2X buffer ...
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bioRxiv - Microbiology 2021Quote: One-step RT-PCR was performed on 2ul total RNA using the SuperScript IV One-Step PCR kit (ThermoFisher, Catalog No. 12594025) either to amplify nucleotides 1232-1583 of the PPRV N gene using published primers NP3 and NP4 (Couacy-Hymann et al 2002 ...
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bioRxiv - Biochemistry 2021Quote: ... The viral titers were determined by the qRT-PCR method using AgPath-ID™ One-Step RT-PCR Kit (Thermo Fisher Scientific) and a sequence of primers and probe for the E gene53 ...
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bioRxiv - Cancer Biology 2022Quote: ... the RNA was subjected to reverse transcription PCR for cDNA synthesis according to the RT-PCR kit (Applied Biosystems, Cat. No. 4368814). The relative gene expression was measured according to 2-ΔΔCT methods ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was reverse transcribed using Superscript III One-Step RT PCR Kit with Platinum® Taq High Fidelity enzyme followed by amplification using Platinum® PCR SuperMix High Fidelity (Invitrogen). A 2200bp amplicon spanning gag and protease was generated by nested PCR as detailed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA was subjected to reverse transcription PCR for cDNA synthesis according to the RT-PCR kit (Applied Biosystems, Cat. No. 4368814). The relative gene expression was measured according to 2−ΔΔCT methods ...
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bioRxiv - Microbiology 2024Quote: ... and the rRT-PCR was performed using the Path-ID™ Multiplex One-Step RT-PCR Kit (Thermo Fisher, Waltham, MA, USA) under following conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... specific mRNA or miR-143/145 was amplified using Power SYBR Green PCR Master Mix (Applied Biosystems) or Taqman Universal PCR Master Mix ...
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bioRxiv - Biochemistry 2023Quote: ... and then washed three times again before detection by SuperSignal™ West Femto Maximum Sensitivity Substrate kit (Thermo Scientific) on Fusion FX instrument (Vilber).
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bioRxiv - Microbiology 2020Quote: One-step RT-PCR was realized with the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Thermo Fisher Scientific) on an iCycler Thermal Cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using the Superscript III one-step RT-PCR system with platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA) under the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... was generated for each subject from 2,000 HIV-1 RNA copies by RT-PCR using SuperScript™ One-Step RT-PCR (Invitrogen, Carlsbad, California) followed by amplification using GoTaq colorless Master Mix (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... according to the manufacturer’s instructions and was used as the template for RT-PCR using Superscript IV One-Step RT-PCR System (Invitrogen, 12594-025). Briefly ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analyses were conducted utilizing the Centers for Disease Control 2019-nCoV N1 primers and probe (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html) and AgPath-ID One-Step RT-PCR reagents (Ambion/ThermoFisher, Waltham, Massachusetts). We included a standard curve of serial dilutions of RNA extracted from SARS-CoV-2 virus stock (107 TCID50/ml ...
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bioRxiv - Microbiology 2023Quote: ... An 800 bp fragment covering the ORF10 region was amplified by one-step RT-PCR using the SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) and the following primers (OL11F ...
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bioRxiv - Neuroscience 2023Quote: Quantification of transcripts was performed by reverse transcription quantitative PCR (RT-qPCR) using the SYBR® Green RT-PCR assay (Applied Biosystems). Amplification ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and an ABI 7900HT RT-PCR machine (Applied Biosystems). Expression levels were normalized to the expression level of the housekeeping gene HMBS and quantitative changes were calculated using the method described in (48) ...
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bioRxiv - Cell Biology 2022Quote: ... TaqMan RT-PCR technology (7500Fast, Applied Biosystems, CA, USA) was used to determine the expression levels of selected target genes with TaqMan sitespecific primers and probes (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... on a StepOne Plus RT-PCR System (Applied Biosystems).
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR Step One Plus system (#8024, Applied Biosystems) was used.
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bioRxiv - Cell Biology 2021Quote: ... and QuantStudio 3 RT-PCR system (Thermo Fisher Scientific). All expression levels were normalized to that of RPS18 mRNA ...
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bioRxiv - Genetics 2022Quote: ... RT-PCR was performed using PowerUP Sybr (ThermoFisher, #A25741) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR was performed using StepOnePlus (Applied Biosystems). Relative expression was calculated using the ΔΔCt method with the reference gene ef1α ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were run on AgPath RT-PCR (Life Technologies) kit on a QuantStudio™ 7 Flex Real-Time PCR System and the relative amounts of FAM and HEX signal used to determine the proportion of Omicron and Delta in the original samples ...
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bioRxiv - Bioengineering 2020Quote: ... with an ABI 7500 RT-PCR System (Applied Biosystems). Data were analyzed with 7500 Software using relative quantification analysis.
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bioRxiv - Genetics 2020Quote: ... RT-PCR was performed using SuperScriptIII Reverse Transcriptase (ThermoFisher) with random primers ...
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bioRxiv - Cancer Biology 2021Quote: ... and RT-PCR Grade Water (Invitrogen, Carlsbad, CA, USA). Using a C100 thermal cycler (BioRad) ...
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bioRxiv - Developmental Biology 2022Quote: ... in a StepOne Plus RT-PCR apparatus (Applied Biosystems). Gene expression was assessed by the comparative CT (ΔΔCt ...
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bioRxiv - Biochemistry 2023Quote: ... A QuantStudio 3 RT-PCR machine (Thermo Fisher Scientific) was used to measure melting temperatures.