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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... is purchased from MakingCosmetics Inc. (USA). 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M solution, Cat. No. J16924-AP) is purchased from Thermo Scientific (USA). Silica beads (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of lysates were incubated with anti-53BP1 for 2 hours at 4°C and subsequently with Protein A magnetic beads (Thermo Fisher Scientific) for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... and treated during 30 minutes at 37°C with 4 µg of RNAse and 2 U of Turbo DNAse (Ambion, Ref AM2239). Another incubation of 30 minutes at 37°C with an additional quantity of Turbo DNAse (2 U ...
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bioRxiv - Physiology 2022Quote: ... Analytical separation was then performed over a 95 min gradient (flow rate of 250nL/min) of 4–25% acetonitrile using a 2 μm EASY-Spray PepMap RSLC C18 75 μm × 250 mm column (ThermoFisher Cat# ES802A) with a column temperature of 45°C ...
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bioRxiv - Microbiology 2023Quote: Cells from a log-phase culture were loaded into an ONIX B04A plate and perfused with fresh LB and 2 µg/mL FM 4-64 (Invitrogen, Cat. #T13320) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... P-1149)-coated 96-well and 24-well plates were seeded with 4 × 104 and 2 × 105 cells accordingly in neurobasal medium (Invitrogen, 21103-049) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... Joachim Herz) for 2 h at 4°C followed by overnight incubation with 20 μL of protein G Ultralink resin (ThermoFisher Cat# 53128). The resins were washed with immunoprecipitation buffer and precipitated proteins were eluted with reducing SDS sample buffer ...
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bioRxiv - Immunology 2024Quote: THJ-529 cells (2 x 104 cells/chamber) were plated in a 4-well chamber slide (Nunc, Thermo Scientific, Waltham, MA, USA), incubated overnight ...
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bioRxiv - Immunology 2024Quote: THJ-529 cells (2 x 104 cells/chamber) were plated in a 4-well chamber slide (Nunc, Thermo Scientific, Waltham, MA, USA), incubated overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Systems Biology 2023Quote: Samples were resuspended in 4% formic acid/2% acetonitrile solution and analyzed on an Q-Exactive Plus MS system (Thermo Fisher Scientific) equipped with an Easy nLC 1200 ultra-high pressure liquid chromatography system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Primary Ab was washed off in PBS triton-X and tissue was then incubated with secondary antibodies at RT for 2-4 hours (Alexa Fluor, Thermo Fisher Scientific). The tissue was then washed again and cover-slipped ...
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bioRxiv - Cell Biology 2023Quote: ... cells at 70-80% confluency were switched to myogenic differentiation medium (DMEM, 4% horse serum, 2 mM Glutamax, 1 mM sodium pyruvate (Life Technologies #11360070), 1 μg/mL insulin (GeminiBio #800122 ...
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bioRxiv - Cell Biology 2023Quote: ... DIV5 mouse hippocampal neurons were incubated with 20 nM Reelin or Mock medium for 2 h after 4 h of incubation in depletion medium (only neurobasal medium (Gibco, Thermo Fisher) plus 1% GlutaMAX) ...
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bioRxiv - Immunology 2024Quote: ... The final eluate of 2 rows was used for SDS gel electrophoresis under reducing conditions on a 4–12% bis-tris polyacrylamide gel (Life Technologies, NP0321BOX), which was blotted onto nitrocellulose membranes (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Quantifoil R2/2 200-mesh copper grids with 2 nm carbon (EM sciences) at 4 °C and 100% humidity using the FEI Vitrobot Mark IV (Thermo Fisher Scientific). The sample was blotted for 5s ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed with 6 mL Accutase for 10 sec and incubated in 4 mL TrypLE select for 2 min/37°C before addition of 200 μL of Neuronal isolation enzyme (Thermo Scientific, 88285) and further incubating 1 min/37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Immunohistochemistry was performed by incubating with primary antibodies (overnight, 4°C) and fluorophore-conjugated secondary antibodies (2 hr, room temperature; ThermoFisher, Jackson ImmunoResearch). Sections were mounted with VectaShield (VECTOR ...
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bioRxiv - Microbiology 2023Quote: ... David Steinhauer) at 4 °C overnight and 2 µg/ml AlexaFluor647 Donkey anti-rabbit IgG secondary antibody (ThermoFisher Scientific Corporation, OR, USA) at room temperature for 45 min ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at 65°C for 5 min and then immediately chilled on ice for at least 2 min before adding 4 μL reverse transcription reaction mix [in 10 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064- 014), 20 mM DTT (Invitrogen - 18064-014) ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Immunology 2023Quote: ... CTLA-4 or PD-1) were suspended in IL-2 medium at 37°C containing either 65nM LysoTracker® Deep Red (ThermoFisher Scientific) or 25nM MitoTracker™ Red CMXRos for 1h or 30min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged 1:4 into new six-well plates with complete DMEM containing 2 μg mL-1 puromycin (Thermo Fisher Scientific). After selection for two weeks ...
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bioRxiv - Biochemistry 2023Quote: ... cells were permeabilized and blocked for 1 h with DPBS containing 2% BSA and 0.1% Triton X-100 and then were incubated with 4 μg/mL anti-FLAG antibody diluted with 2% BSA in PBST with 0.1% Tween-20 (ThermoFisher Scientific, Cat# 85113) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Two hundred and fifty thousand NIH3T3 cells plated in a 6-well plate were transfected in the presence of Lipofectamine 2000 (Invitrogen) (per well 2 µg DNA/4 µl Lipofectamine 2000 in 400 µl Opti-MEM medium; Thermo Fisher Scientific). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... Grids were blotted for 2 s at 100% humidity at 4 °C and flash frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... 5 µl polar metabolite extract were injected in push partial mode using an overfill factor of 1 onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 μm particle size, Thermo Fisher Scientific), additionally this was equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
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bioRxiv - Cancer Biology 2023Quote: ... were transfected with Lipofectamine 2000 (Invitrogen, Netherlands) (per well, 2 µg DNA/4 µl Lipofectamine 2000 in 400 µl Opti-MEM medium; Thermo Fisher Scientific). The next day ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were plated on laminin-coated 18 mm coverslips and treated with 2 μm Rhod2-AM and 4 μm Fluo4-AM (both from Thermo Fisher Scientific) for 15 min at 37°C in external solution (145 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... After selection for 2 weeks in DMEM supplemented with hygromycin B (250 μg/ml; Carl Roth) and blasticidin (4 μg/ml; Thermo Fisher Scientific), single-cell colonies were isolated ...
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bioRxiv - Molecular Biology 2024Quote: ... CCL-2) or HEK-293T (ATCC CRL-3216) cells were seeded in 4-well Nunc Lab-Tek II chamber slide (Thermo Scientific, 154453) coated with Poly-L-Lysine (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... on a rotating wheel for 2 hours at 4°C and 1 hour more incubation with 1.5mg Dynabeads protein G (Thermo Fisher Scientific, 10004A). IP fractions were washed four times with lysis buffer ...
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bioRxiv - Biophysics 2021Quote: 106 T-cells were incubated in T-cell media supplemented with 5 µg ml-1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and HER-2 negative 24JK sarcoma cells were labelled with 5 mM or 0.5 mM CellTrace™ Violet (CTV; Invitrogen-Molecular Probes) respectively in D-PBS supplemented with 0.1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cell Biology 2020Quote: ... and then incubated overnight at 4°C in 2 ml TBST/5% milk with a 1:1,000 dilution of anti-CPY Monoclonal antibody 10A5B5 (Invitrogen, A-6428). The nitrocellulose was then washed 3 times in 20 ml TBST at room temperature for 10 min ...
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bioRxiv - Cell Biology 2020Quote: Proliferation was assessed through incorporation of 5-ethynyl-2’-deoxyuridine (EdU) using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425). One day prior to harvest ...
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bioRxiv - Cell Biology 2020Quote: ... Fixation for 5 min at room temperature was performed by adding an equal volume of PBS 2% methanol-free formaldehyde (Thermo Scientific, USA ...
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bioRxiv - Genetics 2021Quote: ... and primers specific for target and control regions (in which the mark is expected to be enriched (positive controls) or depleted (negative controls)) (Table 2) using the QuantStudio 5 (Applied Biosystems) thermal cycler ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty million cells were diluted in 2 mL of GBSS containing 1% FBS and stained with 5 μg/mL of Hoechst 33342 (Invitrogen H3570) for 1 h at 32 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap x 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm x 50 cm ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were subject to local UVC irradiation at 500 J/m2 and incubated with 20 μM 5-ethynyl-2-deoxyuridine (EdU, ThermoFisher Scientific) and 1 μM 5-Fluoro-2’-deoxyuridine (FUdR ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Differentiation was stimulated by replacing the proliferation medium with differentiation medium (high glucose DMEM with sodium pyruvate, 5% horse serum, 2% L-glutamine, ThermoFisher Scientific). The cells were maintained at 37°C and 5% CO2 throughout differentiation.
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bioRxiv - Systems Biology 2020Quote: ... of the immunodepleted pooled CSF samples were separately dissolved in 100 mM TEAB buffer (625 μL) and labeled with 5 mg of TMT 126 channel reagent (cohort 1 lot# TF266326, cohort 2 lot# SG253447, ThermoFisher Scientific) in anhydrous ACN (256 μL) ...
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bioRxiv - Genomics 2020Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 μm × 50 cm ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Biophysics 2020Quote: 106 T-cells were incubated in T-cell media supplemented with 5 μg ml−1 Fura-2 AM (11524766, ThermoFisher Scientific) for 20 min at 24°C ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were loaded on to 2cm trap column (C18, 5 μm, 100 Å, 100 μm × 2 cm; PN: 164562, Thermo Scientific) and further loaded on 50 cm Easynano spray analytical column (2 μm ...