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Citations for Thermo Fisher :
6751 - 6800 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cells were washed multiple times and incubated with the secondary antibody Alexa Fluor 546 goat anti-rabbit IgG (H+L) (Invitrogen; 1:200 dilution in PBS-BSA-Saponin) in the presence of 1 μM of SiR-DNA for 2 hours in the dark ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 1 h with secondary antibodies (1:1000 dilution in 10% goat serum in PBS) conjugated to Alexa Fluor 568 (Thermo Fisher Scientific, #A-21124 or #A-11011). Cells were then washed three times with PBS and mounted in 5 µl of ProLong(tm ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed three times in PBS and incubated during 4 h in a blocking solution containing a secondary antibody to reveal TH (donkey anti-rabbit Alexa Fluor 594, Invitrogen A21207 lot 1890862 and 2145022 (1:400), RRID ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed and incubated for 1 h at RT with Alexa Fluor 633- and 488-conjugated secondary antibodies (1:500, Invitrogen, catalog #’s A-21070 and A-31553). Sections were mounted onto slides using Vectashield reagent with DAPI (Vector Labs).
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bioRxiv - Cancer Biology 2022Quote: ... The sections were incubated with Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 633 goat anti-mouse IgG secondary antibodies for 1 h (1:200; Molecular Probes by Life Technologies, Waltham, MA, USA). Control slides were incubated with the secondary antibody alone ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific, Thermo Fisher Scientific, Inc., Waltham, MA, USA) on a rotator for subsequent affinity chromatography via the C-terminal 6xHis-tag ...
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bioRxiv - Immunology 2021Quote: ... slides were washed in PBS and then incubated for 1 h at room temperature with secondary antibodies including Goat Anti-Mouse IgG1 Alexa Fluor 488 conjugate (Invitrogen, Thermo Fisher Scientific, A21121, 1:500 in PBS), Goat Anti-Mouse IgM Alexa Fluor 555 conjugate (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... and culture supernatants were harvested after 16 h and assayed for TNF-α levels using a TNF-α human uncoated ELISA kit (Invitrogen, cat. no. 85-88-7346). The assay of CHO-K1 cells (infected with lentivirus of 3A1 and CD80 ...
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bioRxiv - Cell Biology 2022Quote: ... was used as a primary antibody and a goat anti-mouse IgG (H+L) cross-adsorbed antibody Alexa Fluor™ 647 (Thermo Fisher Scientific, A-21235; 1:500) was used as a secondary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... was used together with transferrin-Alexa Fluor 594 or with donkey anti-goat IgG (H+L) secondary antibody-Alexa 594 (Thermo Fisher, cat. no. A-11058, 1:500).
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were incubated for 24 h at 30°C with constant rotary agitation at 200 rpm (MaxQ™ 8000 shaker incubator; Thermo Fisher Scientific Co., Waltham, MA, USA) in 10 mL of DBM medium contained in a 50-mL Erlenmeyer flask with the corresponding antibiotics and chemical inducers needed for induction of the pathway genes ...
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bioRxiv - Physiology 2024Quote: ... tissues/organs were washed with DPBS four times on a rocker over the course of an hour and subsequently incubated overnight at 4°C with a goat anti-rabbit Alexa Fluor® 568 IgG (H+L) secondary antibody (Molecular Probes, Life Technologies, Eugene, OR, USA) diluted 1:200 in 10% NSS made up in DPBS and protected from light ...
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bioRxiv - Microbiology 2024Quote: ... The sheets were blocked with 0.1% Triton X-100 and 10% FBS in 1xPBS for 2 h at 37°C and stained with the following reagents and antibodies: NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI) (Invitrogen, Thermo Fischer Scientific Cat# R37606), Alexa Fluor™ 647 Phalloidin 1:500 (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... phages were incubated with biotinylated pMHC monomer for 1 h followed by incubation with magnetic beads (MyOne Streptavidin T1 Dynabeads, Invitrogen, pre-blocked with 3% BSA in PBS) for an additional hour ...
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bioRxiv - Plant Biology 2020Quote: ... blunt-end PCR products with directional overhangs were cloned into pENTRTM/D-TOPO® vector using the pENTRTM Directional TOPO® Cloning Kit (Thermo Fisher Scientific). The resulting entry clones were inserted into respective Gateway-based destination vectors using GatewayTM LR ClonaseTM II Enzyme Mix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... brain slices were incubated with anti-Calbindin D-28k (Swant, #300, diluted in 1:500) and Alexa Flour 546 (ThermoFisher, diluted in 1:200). The images were collected using a confocal laser scanning microscope (Axio Imager Z2,[Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... AB_2534017, 1:400), Alexa 488 (A21206 RRID: AB_141708 and custom, 1:400) and the nuclear stain DAPI (Invitrogen Molecular Probes D-21490, 1:5000) for visualization by epifluorescence microscopy.
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bioRxiv - Neuroscience 2021Quote: ... AB_2534017, 1:400), Alexa 488 (A21206 RRID: AB_141708 and custom, 1:400) and the nuclear stain DAPI (Invitrogen Molecular Probes D-21490, 1:5000) for visualization by epifluorescence microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with non-targeting siRNA (Dharmacon, cat# D-001810-10-05) or siRNA targeting Cdkn1a (Dharmacon, cat# L-058636-00-0005) using Lipofectamine RNAiMAX (Thermo Scientific, cat# 13778-075) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... medium was removed and cells were washed once with Dulbecco’s Phosphate Buffered Saline (D-PBS) and incubated with TrypLE™ Select Enzyme (Thermo Fisher scientific, cat. 12563029) solution (300 μl/well ...
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bioRxiv - Neuroscience 2020Quote: All neuronal tracings were done by using combinations of tetramethylrhodamine-biotin dextran (Microruby, 3,000 MW, lysine fixable; Molecular Probes, Eugene, OR, USA, D-7162) and Alexa Fluor 488 dextran (10,000 MW ...
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bioRxiv - Physiology 2022Quote: ... Cells were cultured on poly-D-lysine–coated 10 cm cell culture dishes (MilliporeSigma, Burlington, MA) in a DMEM-F12 medium (Thermo Fisher Scientific, Waltham, MA) at 37°C in the presence of 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Mouse cytokine levels in vaccinated animals (IL-4, IL-10, IL-12, and IFN-γ) was measured by ELISA following the supplier’s protocol (Invitrogen or R&D Systems).
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bioRxiv - Cell Biology 2022Quote: ... In mock experiments cells were transfected with equal amount of ON-TARGETplus Control Pool Non-Targeting pool (D-001810-10-05, Dharmacon, CO, USA) or Silencer Select Negative Control #1 siRNA (4390843, Ambion, Thermo Fisher Scientific, MA, USA). All plasmid transfections were performed using Nucleofactor Kit R with the Nucleofactor 2b Device (Lonza ...
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bioRxiv - Plant Biology 2021Quote: The PVY CP encoding cDNA was amplified by reverse transcription-PCR from total RNA extracted from PVY-infected tobacco leaf tissue as described earlier (Grech-Baran et al., 2020) and inserted into the pENTR/D-TOPO vector (Thermo Fisher Scientific, Waltham, US). The sequence of ...
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bioRxiv - Immunology 2021Quote: ... 200–300 ng of RNA were reverse transcribed using oligo d(T) or random primers and Superscript III (Thermo Fisher Scientific, catalog no. 18080051) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... or the non-targeting control siRNA pool (Horizon, #D-001810-10-05) 24 hours after seeding the cells using DharmaFECT 2 (Thermo Scientific, #T-2002-03) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... using crude extracts from plant leaves and monitoring the quantity of 4-methylumbelliferone products generated from the substrate 4-methylumbelliferyl-β-D-glucuronide (Duchefa) on a fluorometer (Thermo Scientific fluoroskan ascent).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 31 VeroE6 cells were plated at 10,000 cells per well of poly-D-lysine-coated 96-well plates (Thermo Fisher Scientific, Rochester, NY, USA). For pretreatment assays ...
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bioRxiv - Cell Biology 2020Quote: The sequences of PNDD0 and PNDD1 were synthesized and cloned in a pET100/D-TOPO vector using gene synthesis (Thermo Fisher Scientific, Carlsbad, CA). H1 peptide sequence is underlined.
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bioRxiv - Developmental Biology 2020Quote: ... and HmlΔ-Reverse primer: 5’-GTTTAATTGTATACACAGGAAAATC-3’) were amplified from Drosophila genomic DNA and ligated into the pENTR™/D-TOPO™ vector (Invitrogen: Cat#K240020) for Gateway cloning ...
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bioRxiv - Cell Biology 2020Quote: ... n-dodecyl-β-D-maltoside and assessed for OxPhos complex content using a Native PAGE Novex Bis-Tris Gel system (Invitrogen, Carlsbad, CA, USA). The separated proteins were transferred to polyvinylidene fluoride membranes ...
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bioRxiv - Cell Biology 2021Quote: The monoclonal antibodies, anti-CD9 Alexa Fluor® 488-conjugated (R & D systems, Canada) or anti-CD63 Alexa Fluor® 488-conjugated (Invitrogen, ThermoFisher Scientific) or anti-CD81 Alexa Fluor® 488-conjugated (R & D systems ...
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bioRxiv - Developmental Biology 2021Quote: ... for amplification of 100ng of cDNA template from N/D/S cells either treated with MS351 or vehicle using Power SYBR Green Master Mix (Thermo Fisher Scientific Cat. 4368577) qRT PCR protocol14 ...
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bioRxiv - Cell Biology 2022Quote: ... then room temperature blocking solution (3% BSA in D-PBS) containing secondary antibody (IgG2b Cross-Adsorbed Goat anti-Mouse, Alexa Fluor™ 555 [Invitrogen, 10412832], 1:2000) was added using Dragonfly Discovery liquid dispenser and incubated for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... The fragments were cloned into entry vector pENTR™/D-TOPO™, and transferred to pB7FWG2.0 destination vector (Karimi et al., 2002) with Gateway™ LR Clonase™ II (Thermo-Fisher Scientific, Germany). Site-directed mutagenesis was carried out to specifically mutate the amino acid residues of interest.
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... HRE2 and RAP2.3 in pENTR-D/TOPO (kindly provided by Prof. Margret Sauter, Christian-Albrechts University, Kiel, Germany) recombined into pDEST22 (Thermo Fisher Scientific, Waltham, USA), possessing the GAL4 activation domain ...
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bioRxiv - Molecular Biology 2024Quote: ... 2b–f, 3b,c and 4b,c and Extended Data Fig. 1b,c, 2a–d, 3a and 4d,e; Gibco, Extended Data Fig. 2b) media supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested by scraping in cold PBS and prepared for 1-D gel analysis using Click-IT L-azidohomoalanine protein labeling reagents (Life Technologies C10102, C10276, B10185) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transiently transfected with mitochondrial matrix targeted RCaMP to measure changes in [Ca2+]mt then plated on Poly-D-Lysin coated 25 mm coverslips (Thermo Fisher Scientific, 25CIR-1.5). To measure changes in the [Ca2+]cyt cells were loaded with 2 μM Fura2AM (Moleculer probes ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical mixed glia were seeded on 10 microgram/milliliter (ug/mL) poly-D-lysine-coated T25 flasks (minimum two brains per flask) and grown in DMEM/F12+GlutaMAX™ (Gibco, Cat # 10565-018) supplemented with 10% fetal bovine serum and 1% Pen/Strep until confluency (∼1 week) ...
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bioRxiv - Plant Biology 2023Quote: ... This was done by digesting the ONSEN RT plasmid with XmaI and PacI and subcloning the fragment (containing the endogenous ALS gRNA gene) into a pENTR/D-Topo vector (Thermo fisher Scientific, Waltham, MA). The resulting plasmid was PCR amplified with a primer pair to change the ALS sgRNA spacer sequence to one of three different CRY2 sequences (sgRNA#1 ...
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bioRxiv - Neuroscience 2023Quote: ... Freshly dissected tongue was put dorsal side down into a 3-D printed reservoir and was gently pressed against the bottom of the reservoir by a cell strainer (Fisher Scientific, 22-363-549). 4% PFA was added into the reservoir to fix the tissue at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis, MN; Penicillin-Streptomycin from Gibco, Thermo Fisher Scientific) for 24 hours and then treated with either free drug (FD ...
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bioRxiv - Cell Biology 2023Quote: ... The fibers were separated using a wide bore p1000 pipette to yield single quadriceps myofibers in D-MEM Medium (Invitrogen, Waltham, MA, 21063-029), FBS (2 %) ...
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bioRxiv - Neuroscience 2023Quote: ... and replated at low density onto 12 mm poly-D-lysine-coated glass coverslips (GG-12-pdl; NeuVitro, Vancouver, WA) in 24-well tissue culture plates (FisherBrand FB012929; Thermo Fisher Scientific, Waltham, MA), for patch-clamp electrophysiology ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-rat DC217 (1:200, Axon Neuroscience R&D SE, Bratislava, Slovakia) and polyclonal anti-rat pThr212 (1:1000, Invitrogen Life Technologies, Carlsbad, CA). For total tau ...
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bioRxiv - Microbiology 2023Quote: ... mutans was visualized via Alexa Fluor 555 (Life Technologies; B40955. For another antibody pair, fungal β-D glucan was visualized via Alexa Fluor 647 (Life Technologies; B40958) and Gram positive bacteria LTA was visualized via Alexa Fluor 647 (Life Technologies ...