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Citations for Thermo Fisher :
6301 - 6350 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... plates were coated with 50 µl of capture anti-FLAMOD monoclonal antibody (Mab, 9H10-R2-2E8 mouse IgG1 - lot vt220802-7034) at 5µg/ml in D-PBS 1X (PBS, Gibco 14190-094) and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... cells without added Aβ served as negative control (subtracted from all values) while cells incubated with the phagocytosis inhibitor Cytochalasin D (20 nM in DMSO, ThermoFisher Scientific) 2 hours prior Aβ incubation inhibited phagocytosis and was used for normalization of the data.
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bioRxiv - Neuroscience 2024Quote: ... and seeded at assay-dependent concentrations onto poly-D-lysine-coated plates in BMDM culture medium [RPMI-1640 (Gibco; Cat# 61870036), 10% HyClone FBS (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: The coverslips with the fixed cultured primary hippocampal neurons or HEK293 cells were briefly washed in D-PBS (Gibco, 14190-094). The coverslips were washed three times 5 min in D-PBS containing 0.1% Triton X-100 at RT to permeabilize the cells ...
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bioRxiv - Physiology 2024Quote: ... Adult mosquitoes were kept in the 30 x 30 x 30 cm cages and provided with deionized water and 10% sucrose solution ad libitum before sugar treatment (sucrose, D- and L-arabinose: Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... of AtCCA1 was amplified from cDNA of Arabidopsis thaliana (Columbia-0) and cloned into the vector pENTRTM/SD/D-TOPO® (Invitrogen) and then transferred to the gateway-compatible pMDC32 binary vector and pMDC43-green fluorescent protein (GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... MEF and human skin fibroblasts were cultured in Dulbeccós modified Eagle medium (DMEM) (1X) with high glucose (4.5 g/L D-Glucose) and sodium pyruvate (0.11 g/L) (Thermo Fisher Scientific #21969), supplemented with 1% glutamine (Gibco #25030081) ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was extracted from 30–40 heads of 3–8-d-old males using TRIzol (15596026, Thermo Fisher Scientific, USA) as one sample (210–320 heads were used for each genotype) ...
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bioRxiv - Plant Biology 2024Quote: ... of MIR319C were amplified from pMIR319C-P4P1R entry clone using Taq DNA polymerase and cloned into pENTR/D-TOPO vector (K240020, Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... containing 10 % (vol/vol) fetal bovine serum (FBS; Wisent, 086–15) and antibiotics (1 d% penicillin/streptomycin, Thermo Fisher Scientific, 15,070,063).
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bioRxiv - Neuroscience 2024Quote: ... before MGE cells were dissociated in cold Dulbecco‘s Modified Eagle Medium (DMEM) with additional L-glutamine and 4.5 g/L D-glucose (Thermo Fisher Scientific, U.S.A.), 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2024Quote: ... The protein fractions were then loaded on the Step-affinity column equilibrated with binding buffer (with adjusted imidazole composition) and eluted after washing with 50 mM D-(+)-Biotin (Thermo Scientific) after 1 h incubation on bead ...
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bioRxiv - Cell Biology 2024Quote: ... cells were co-transfected with 0.5 µg of each plasmid pIRO-D and COPI cDNA well using Lipofectamine 3000 (Thermo Fisher Scientific) according to manufacturer’s instructions and transfection reagent-containing media was replaced with complete DMEM at 3 h.p.t. ...
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bioRxiv - Bioengineering 2024Quote: ... chips were washed thrice with 0.5% Tween-20 in D-PBS and 20 µg/mL Hoechst 33342 (H3570, Thermo Fisher Scientific) in D-PBS was added to all chips ...
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bioRxiv - Cell Biology 2024Quote: ... the PpROP1 coding and 3’UTR sequence were amplified from cDNA and cloned into pENTR™/D-Topo (Thermo Fisher Scientific) yielding pFAU253 and mutated using PCR-based site-directed mutagenesis followed by DpnI digestion generating pFAU256 (PpROP1Q64L ...
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bioRxiv - Cell Biology 2024Quote: Cells were cultured on top of glass coverslips previously covered with Poly-D-Lysine (1 mg/mL for MCF-10A, P6407, Sigma-Aldrich and 100 µg/mL for EpH4, A3890401, Gibco). Cells were fixed with PFA 4% in PBS for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... the expression of H6-mEGFP-Mms6 Mms6[112-132] was induced with 0.5 mM IPTG (isopropyl β-D-1-thiogalactopyranoside, Thermo Scientific) at OD600 = 0.6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Jhen-Wei Ruan (NCKU) and were cultured with DMEM/F12 (D/F12) medium (#11330-032, Gibco/Life Technology, Carisbad, CA, USA) Both cell culture medium were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were then washed in PBST and incubated with secondary antibody anti-mouse IgG (H+L) coupled to horseradish peroxidase (1/20000; ThermoFisher Scientific Cat# 32230, RRID:AB_1965958). Proteins were revealed by SuperSignal™ West Pico Chemiluminescent Substrate (ThermoFisher Scientific Cat# 34580 ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated for 1 hour at RT with goat anti-mouse and goat anti-rabbit IgG (H + L) secondary antibodies conjugated to fluorophores (Alexa Fluor 488 nm, 568 nm and/or 594 nm; 1:500; Life Technologies, Carlsbad, CA, USA). Unbound antibodies were rinsed and conjugated fluorophores protected with Fluoromount-G (Southern Biotech ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies and stains: Goat anti-Rabbit IgG (H+L) Alexa 488 and 594 (1:1000 cells, 1:500 tissue Invitrogen; A-11008, A-11012), Goat anti-Mouse IgG1 Alexa 488 (1:500 Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS 3 times for 5 min and then incubated for 2 h at RT in blocking solution with the corresponding Alexa-conjugated secondary antibodies (Thermo Fisher Scientific; Massachusetts, USA). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... anti-rabbit HRP antibody (1:5000) or with anti-goat HRP antibody (1:5000) was performed for 1 h and ECL (Pierce, Thermo Scientific, Rockford, IL, USA) visualized detection ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with PBS and further incubated in the dark for 2 h with Alexa Fluor® 488-conjugated donkey anti-rabbit IgG secondary antibody (Thermo Scientific; 1:500). Then ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Synthesis of cDNA was performed according to manufacturer’s protocol using the RevertAid H Minus First Strand cDNA synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA), and it was stored subsequently at - 20°C until further use ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated for 30 minutes at room temperature with a 1:1000 dilution of donkey anti-rabbit IgG (H+L) secondary antibody conjugated to Alexa Fluor® 647 (ThermoFisher-Invitrogen, catalog A-31573) in blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hour at room temperature in darkness with appropriate secondary antibodies: Alexa Fluor 488 donkey anti-mouse IgG (H+L) (1:400, A21202, Thermo Fisher Scientific, Mass., USA), Alexa Fluor 555 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2021Quote: ... Isoproterenol responsiveness was read out after a 2 h feeding phase of on-chip adipocytes with the fluorescently labeled fatty acid (BODIPY™ 500/510 C1, C12; D3823, Thermo Fisher Scientific Inc.) by analyzing the release of fatty acids from the adipocytes ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were washed three times with 0.05% Tween 20/PBS and then incubated for one hour in the secondary antibody Alexa Fluor® 488 goat anti-Mouse IgG / IgA / IgM (H+L) (Molecular probes 1:500) combined with the secondary antibody Alexa Fluor® 594 goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2020Quote: ... Double-stranded cDNA was synthesized from the purified polyA+ RNA using the Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific Inc., USA) and random hexamer primers ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 hour at RT with goat anti-mouse and goat anti-rabbit IgG (H + L) secondary antibodies conjugated to fluorophores (Alexa Fluor 488 nm, 568 nm and/or 594 nm; 1:500; Life Technologies, Carlsbad, CA, USA). Unbound antibody was rinsed ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with the HRP-conjugated anti-rabbit secondary antibodies at room temperature for 1 h and developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific, #32106). The images were captured on ChemiDoc™ Touch Imaging System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... The free fatty acid were analysed using ultra-performance liquid chromatography (Waters, Acquity I Class System) coupled with mass spectrometer (Q-Exactive Thermo Fisher Scientific, H-ESI). Processing of chromatograms ...
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bioRxiv - Systems Biology 2021Quote: ... Metabolites were separated through a HyperREZ XP Carbohydrate H+ 8 μm column coupled with a HyperREZ XP Carbohydrate Guard (Thermo Fisher Scientific, Waltham, MA). The analysis conditions were ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed 5 times and incubated with goat anti-human IgG (H+L) antibody conjugated with ALP (Thermo Fisher, a18808, 1:5000) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hour at room temperature in darkness with appropriate secondary antibodies: Alexa Fluor 488 donkey anti-mouse IgG (H+L) (1:400, A21202, Thermo Fisher Scientific, Mass., USA), Alexa Fluor 555 donkey anti-rabbit IgG (H+L ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... The fluorescent secondary antibodies used in this study were Alexa Fluor 568 goat anti-human IgG(H+L) (Molecular Probes, A-21090, 1:1000), Alexa Fluor 488 goat anti-mouse IgG(H+L ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-mouse and/or goat anti-rabbit AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500) secondary antibodies ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... The anti-FLAG antibody was subsequently detected with goat anti-mouse IgG (H+L) conjugated to horseradish peroxidase (HRP) to facilitate chemiluminescent detection (Invitrogen 32430, 1:1,000 dilution). Chemiluminescent signal was produced with Pierce ECL substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µM EdU was administered for 1 h prior to fixation and detected using the Click-iT™ Plus EdU Alexa Fluor™ 555 Imaging Kit (ThermoFisher Scientific, C10638) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then incubated for 2 h in one of the secondary antibodies: donkey anti-mouse Alexa 647 (Thermo Fisher Scientific, A-31571) for GAD67 ...
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bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
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bioRxiv - Immunology 2022Quote: ... The transfected cells were cultured at 37°C for 24 h and then collected and lysed using protein lysis buffer (Thermo Fisher Scientific, Cat #: 87787). Aliquots of lysate (20 μg protein ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed with PBST and incubated with goat anti-mouse IgG (H+L) secondary antibody conjugated with horseradish peroxidase (Thermo Fisher, 31430, 1:10,000) on a shaker at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were subsequently incubated by secondary antibodies for 1 h at room temperature and developed with Enhanced Chemiluminescence (ECL) (Thermo Fisher Scientific, Waltham, MA). Protein bands were quantified using ImageJ (https://imagej.nih.gov/ij/) ...
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bioRxiv - Neuroscience 2020Quote: Each organotypic slice was incubated for 1 h prior to imaging with the fluorescent Ca2+-indicator dye Fluo-4-AM (Molecular Probes, Invitrogen, Carlsbad, CA, USA). The conditions for incubating the dye were standardized across different slices to avoid uneven dye loading ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed twice and stained with 1:50 of goat anti-rabbit IgG (H+L) Alexa Fluor 647 (Thermo Fisher Scientific Cat#A21244) for 30 minutes at 4°C followed by two washes and diluted in 100 μL of staining buffer ...
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bioRxiv - Immunology 2021Quote: ... the virus-serum mixture was added to 1 x104 Vero or Vero E6 cells seeded 3 h before in 96 well plates (Thermo Fisher Scientific, Ulm, Germany). Cells were incubated for 4 days at 37°C in a humidified atmosphere containing 6% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were incubated for 1 h at RT with appropriate secondary antibodies conjugated to Alexa Fluor 488 or 594 (Molecular Probes, ThermoFisher 1:500) diluted in 0.1% BSA ...
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bioRxiv - Microbiology 2022Quote: Formalin-fixed gut segments from germ-free or specifically associated mice were paraffin-embedded and 5 μm sections cut for staining with hematoxylin and eosin (H&E: Thermo Fisher Scientific, Waltham, MA) as described (21) ...