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Citations for Thermo Fisher :
6601 - 6650 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times with blocking solution followed by incubation with Alexa 488-conjugated goat anti-rabbit IgG (H+L) (Life Technologies:# A-11034; 1:5000) for 2.5 h ...
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bioRxiv - Microbiology 2022Quote: ... plates were washed three times and further incubated with 100 µL horseradish peroxidase (HRP)- conjugated Goat anti-Feline IgG (H+L) secondary Antibody (Thermo Fisher Scientific, Waltham, MA, USA). The plates were incubated for 1 hour at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... Muscle pieces were then washed with 0.5% PBST and incubated with fluorescein-conjugated secondary antibodies including: goat anti-mouse IgG H+L (Alexa Flour 488, Invitrogen, #A-11029, 1:400 dilution), goat anti-rabbit IgG H+L (Alexa Flour 488 ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were incubated with Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 633 goat anti-mouse IgG secondary antibodies for 1 h (1:200; Molecular Probes by Life Technologies, Waltham, MA, USA). Control slides were incubated with the secondary antibody alone ...
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bioRxiv - Physiology 2020Quote: ... Slides were washed twice with PBS and then incubated for 1 h at room temperature with Alexa Fluor® 488-labeled goat anti-rabbit IgG (1:500; Molecular Probes, Inc., Eugene, OR) diluted in PBS with 2% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 1 h with 4 μg/mL secondary anti-IgG antibodies labeled with Alexa-594 and Alexa-488 (Molecular Probes, Eugene, Oregon, United States).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue samples were dried at 60°C for 24 h r and then all samples were pre-digested with 5 mL ∼65% nitric acid (TraceMetal™ Grade, Thermo Fisher Scientific, Waltham, MA) and 1 mL ∼30% hydrochloric acid (TraceMetal™ Grade ...
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bioRxiv - Molecular Biology 2021Quote: ... 1-3 μg RNA was reverse transcribed with the RevertAid H Minus First Strand cDNA synthesis kit and Oligo(dT)18 primers (both Thermo Fisher Scientific, Bleiswijk, the Netherlands), following the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... cover slips were incubated for 1 h at room temperature with mouse anti-mouse Keratin 14 (LL02, Thermo Fisher, MA5-11599, 1:100 – 1:10), rabbit anti-mouse Keratin 5 (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 hour respectively with the secondary antibodies goat anti-rabbit IgG (H+L) conjugated to Alexa fluor 594 (Molecular Probes A-11012, 1:400); goat anti-mouse IgM conjugated to Alexa fluor 594 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultured in RPMI (24 h, 37°C, 5% CO2) and stained with MitoTracker Red FM (ThermoFisher Scientific, M22425; 30 min, 37°C). Grids were vitrified by plunge-freezing using a Leica EM GP2 grid plunger (Leica Microsystems ...
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bioRxiv - Immunology 2022Quote: ... at 37°C and 5% CO2 for 2 h in 12-well Nunclon™ Delta surface-treated flat bottom plates (Thermo Fisher Scientific, Waltham, MA). Non-adherent cells were removed by gently washing the cells with pre-warmed culture medium ...
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bioRxiv - Microbiology 2022Quote: ... HSAEC1 cells were seeded in 96-well plates at a density of 1 × 104 cells/well and after 24 h were transiently transfected using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA). After an incubation at 37°C for 36 h post transient transfection ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed in TBS-T and incubated with secondary antibodies at room temperature for one hour (AlexaFluor 594 goat anti-rabbit IgG (H+L) (Invitrogen, Ireland, A-11012, 1:500), Cy®5 goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... slides were washed and incubated 3 h with secondary antibodies: Alexa Fluor 488 (Anti-rabbit, 1:1000, Invitrogen, Themo Fisher Scientific, Slangerup Denmark, #A11008, AB_143165), Cy3 (monoclonal donkey anti-rabbit 1:600 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were again washed in 0.01M PBS and incubated for 1 h at room temperature with the secondary conjugated antibody (1:1000, Invitrogen: goat anti-rabbit Alexa Fluor 488). The sections were washed in 0.01M phosphate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated for 1 h at room temperature with Alexa Fluor 488-conjugated donkey anti-rabbit secondary antibody (1:200; Invitrogen, A-21206, Waltham, MA, USA). The Gi-DREADD or stained brain sections were mounted on resin-coated slides and dried slightly ...
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bioRxiv - Molecular Biology 2022Quote: Primary mouse hepatocytes were treated with 100 nM insulin (MilliporeSigma, Burlington, MA, USA) for 3 h in glucose-free DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 20 mM sodium lactate (MilliporeSigma ...
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bioRxiv - Microbiology 2023Quote: ... 500 ng of RNA were used for the synthesis of cDNA using the RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Fisher Scientific, Waltham, MA, USA) with random hexamer primers ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge, Thermo Fisher Scientific, Darmstadt, Germany). The fluorescence intensity of the supernatant was measured measured (485 nm excitation ...
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bioRxiv - Molecular Biology 2024Quote: ... The slides were washed thrice with PBS and blocked with 5 % milk inside a humid chamber for 1 h before being incubated with α-V5 mouse primary antibody (Thermo Fisher Scientific – 1:400) overnight at 4°C ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... 1% v/v TEMED and 0.05% w/v APS) and electrophoresed at 130 V for 2 h with NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific, Cat no. NP0001-02). At the end of electrophoresis ...
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bioRxiv - Developmental Biology 2024Quote: ... The following secondary antibodies and dilutions were used: Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488 (1:500, Thermo Fisher Scientific, catalog #A-21206), Donkey anti-Rat IgG (H+L ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated for 2 h with secondary antibodies conjugated to Alexa-488 (1:2000; Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA). For the D7F7 antibody ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies used for IF are as follows: donkey anti-rat IgG (H+L) highly cross-adsorbed secondary antibody (Alexa Fluor 488, 1:500, Thermo Fisher Scientific, A21208; RRID: AB_2535794), and donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 ng DNase-treated RNA was then used for cDNA synthesis in a 10 µ L reaction using Maxima H Minus cDNA Synthesis Master Mix (Thermo Fisher Scientific, Cat. M1662). 4.8 µL 10-fold diluted cDNA was subsequently used in a 10 µL qPCR reaction with 500 nM forward and reverse primer ...
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bioRxiv - Molecular Biology 2023Quote: ... First-strand cDNA synthesis was performed on DNA-free RNA with random hexamer primers and/or (for NATs) gene-specific primers using RevertAid H Minus Reverse Transcriptase (EP0451, Thermo Fisher Scientific, Waltham, Massachusetts, US) or Superscript IV (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The plates were blocked for a minimum of 1 h at 37°C in serum-free AIM-V™ medium (Thermo Fisher Scientific, cat.: 12055091). To each well ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected 16–24 h prior to the experiment using 200 ng plasmid DNA per dish with Turbofect (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... 800 ng RNA was used to generate cDNA with the Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA). For DNA-qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 2 hours at room temperature with goat anti-chicken IgY (H+L) Alexa Fluor™ 488 (Invitrogen, A-11039, 1:1000) diluted in TBS with 10% Serum ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed three times in PBS and were incubated in the dark for 1 h at room temperature with goat anti-mouse Alexa Fluor® 488-conjugated antibody (Invitrogen, USA, 1:200 dilution) or goat anti-rabbit Alexa Fluor® 488-conjugated antibody (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Pons sections were stained with Hematoxylin and Eosin (H&E, Cat. No. 26041-06, EMS, Hatfield, PA, USA and Cat. No. E511-100, Thermo Fisher Scientific, Tempe, AZ, USA). Sequential sections were incubated in antisera raised against MAP2 (1:8,000 ...
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bioRxiv - Cell Biology 2023Quote: Reverse transfection of cells with ON TARGETplus siRNAs (Dharmacon, final concentration: 10 nM) was performed for 12 h with Lipofectamine™ RNAiMAX (Thermo Fisher Scientific, cat.no. 13778030) as described by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... incubated at room temperature for 2 hours with Alexa Fluor® 647-conjugated donkey anti-mouse IgG (H+L) antibodies (1:500, Invitrogen, # A-31571, RRID: AB_162542) and 0.8 µM Hoechst 33342 at room temperature ...
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bioRxiv - Genetics 2024Quote: ... The DNA-bound beads were then incubated with 60 μg nuclear extract from BL2 cell line or PBMC or no nuclear extract for 1 h at room temperature in LightShift Chemiluminescent EMSA Kit reaction buffer (Thermo Fisher Scientific, catalog number 20148X). After washing and separation ...
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bioRxiv - Microbiology 2024Quote: ... VN and Strand-Switching primers from the cDNA-PCR Sequencing Kit (ONT #SQK-PCS109) were used with Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA) for reverse transcription and strand switching with the following parameters ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then washed three times followed by an incubation for 1 h with the following secondary antibodies: goat anti-rat Alexa Fluor 647 antibody (Thermo Fisher, #A78947, 1 µg/ml), goat anti-mouse Alexa Flour 488 antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... antibodies overnight at 4°C and then incubated with Alexa Fluor 488-donkey anti-rabbit IgG (H + L) (1:200, Life Technologies, Gaithersburg, MD, USA, #A21208) for 1h ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibody incubation was performed in blocking solution for 2 h at room temperature (goat anti-mouse IgG1, Alexa Fluor 488, Life Technologies A21121; 1:500 dilution). Counterstaining was performed by adding NeuroTrace 640/660 (ThermoFisher Scientific #N21483 ...
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bioRxiv - Neuroscience 2024Quote: ... We then washed and incubated slices for one hour with secondary antibodies diluted at a ratio of 1:200 (Alexa Fluor 488 goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific Cat# A-11008); Alexa Fluor 594 goat anti-mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: ... 1-2 µg of total RNA from each sample was reverse transcribed to cDNA using RevertAid H Minus Reverse Transcriptase (Thermo Fisher Scientific, catalog no. EP0452). The resulting cDNA samples were diluted ...
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bioRxiv - Neuroscience 2024Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific, Waltham, MA, USA) in PBS containing 0.1% Triton X-100 and 1% normal goat serum for 2 days at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were incubated for 6 h at 37 °C in the presence of eBioscience™ Protein-Transport-Inhibitor-Cocktail (00-4980-93, Thermo Fisher Scientific, 1:500), followed by stimulation with the RBD peptide pool (5 μg/well ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies used in this study are: AlexaFluor-546 goat anti-rabbit IgG (H+L) (1:500, Thermo Fisher, Cat# A-11035; RRID: AB_2534093).
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections were stained with hematoxylin and eosin (H&E) or mounted with 4′,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (Thermo Fisher Scientific, Waltham, MA, USA). They were imaged with SLIDEVIEW VS200 Slide Scanner (Olympus ...
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bioRxiv - Biochemistry 2024Quote: ... samples were resuspended in ice-cold FACS buffer containing 2 µg/mL goat anti-mouse IgG (H+L) AlexaFluor™ 488 (Thermo Fisher Scientific, A-11001). After incubation and washing as described before ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used for immunofluorescence: Alexa Flour 488 donkey anti-rabbit IgG (H+L) (Thermo Fisher Scientific, USA, #A21206, IF-1:250).
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were injected into a C-18 trap column (Acclaim PepMap100, 75 μm i. d. x 2 cm, C18, 3 μm, 100 Å, Thermo Fisher Scientific) and further separated on a C-18 analytical column (Acclaim PepMap100 ...
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bioRxiv - Cancer Biology 2021Quote: ... and further separated on a C-18 analytical column (Acclaim PepMap100, 75 μm i. d. x 50 cm, C18, 2 μm, 100 Å, Thermo Fisher Scientific) using a multistep gradient with buffer A (0.1% formic acid ...