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Citations for Thermo Fisher :
6451 - 6500 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cell viability was determined after 48 h of treatment by FACS analysis of cellular exclusion of SYTOX Blue Dead Cell Stain (Life Technologies Cat No S34857) using an LSR-Fortessa (BD) ...
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bioRxiv - Genetics 2024Quote: ... After washing with PBST 3 times for 5 minutes the blot was incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody HRP (Thermo Fisher Scientific cat# 31460) at room temperature with shaking for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... for 1 h and blotted with primary antibodies (1: 2000 anti-FLAG, Millipore Sigma, F3165, and 1:500 anti-Rpl7, ThermoFisher Scientific, A300-741A) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 204 g of frozen anchovy waste in the blender jar of an electric blender was added with a first aliquot of 106 g of d-limonene (Acros Organics, 96%) refrigerated at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cloning of the pET151-YtgCR-3xFLAG vectors was performed following manufacturer protocols for TOPO directional cloning into the pET151-D/TOPO base vector (Invitrogen, Thermo Fisher Scientific). The YtgCR sequence was amplified from C ...
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bioRxiv - Cell Biology 2021Quote: ... or siGENOME SMARTpool siRNA D-019800-17 DSP (Dharmacon, Lafayette, CO) (siDP1) or stealth siRNA oligonucleotides 5′-CAGGGCUCUGUCUUCUGCCUCUGAA-3′ from Life Technologies (siDP2) using the Amaxa Nucleofector System (Lonza ...
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bioRxiv - Developmental Biology 2021Quote: ... and truncated versions of ABIG1 were amplified from cDNA and sequenced before cloning into GATEWAY® pENTR/D-TOPO® (Invitrogen, CA). The entry clones were subcloned into both pDEST32 and pDEST22 vectors (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... counted and re-seeded on poly-D-Lysine coated dishes containing Rat astrocytes in neuronal medium (B27 Electrophysiology medium (Gibco, A14137-01) supplemented with 1× MEM non-essential amino acids ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and immersed and incubated in the dark in staining solution 1 mM 5-bromo-4-chloro-3-indolyl-β-D-glucuronicacid (X-Gluc, Thermo Scientific), 50mM sodium phosphate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were collected on gelatin-coated glass slides and stained with solution D and E before being dehydrated through graded alcohols and mounted using Permount (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2020Quote: ... the MSL10 gene was amplified from genomic wild-type Col-0 DNA using gene-specific primers listed in Table S1 and cloned into pENTR/D-TOPO entry vector (Thermo Fisher Scientific), then recombined into pBGW destination vectors (Karimi et al. ...
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bioRxiv - Microbiology 2020Quote: ... 30,000 cells/well were cultured at 37°C in 5% CO2 as a monolayer on coverslips (d = 12 mm; Paul Marienfeld GmbH & Co. KG) in 24 well plates (#08-772-1H, Thermo Fisher Scientific) with DMEM ...
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bioRxiv - Microbiology 2021Quote: ACPL-GFP D10 and NF54 PfMev parasites were synchronized with 5% (w/v) D-sorbitol for 10 minutes at room temperature and returned to culture in 10 µM fosmidomycin (Invitrogen Life Technologies F23103), 100 nM atovaquone (Caymen Chemicals 23802) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were washed 3 times during 30 minutes with PBS supplemented with Triton X-100 (0.1%) and then incubated overnight with BODIPY 493/503 (Molecular Probes, D-3922) 15 μg/mL diluted in PBS ...
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bioRxiv - Biophysics 2021Quote: Coverslips for live-cell imaging were prepared by cleaning round glass coverslips (d=25 mm, 0.17 mm thickness) (Menzel Gläser, Thermo Fisher Scientific, Waltham, MA) for 1 min in O2 plasma and incubating them with fibronectin (2 μg/mL ...
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bioRxiv - Biophysics 2021Quote: ... using the Äkta pure purification system (Cytiva, Marlborough, MA) and eluted by buffer A-200 containing 5 mM D-desthiobiotin (Thermo Fisher Scientific). Pooled eluate fractions were adjusted to 150 mM NaCl and purified on a 5 ml HiTrap Heparin column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were dissociated by papain digestion and plated on poly-D-lysine-coated (0.01 mg/mL) 8-well Lab-Tek II chambered cover glass (Thermo Fisher Scientific, #Z734853). Specifically ...
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bioRxiv - Neuroscience 2021Quote: ... and the pellet was gently resuspended in 800 μl of wash buffer before 7-aminoactinomycin D (Thermo Fisher Scientific, 00-6993-50) staining and fluorescence-activated cell sorting (FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA encoding mouse FAK (Ptk2) without EGFP was PCR amplified from this plasmid and subcloned into pENTR/D-TOPO entry vector (Thermo Fisher Scientific). Entry vector is then further cloned into lentiviral destination vector pDest-659 along with mPol2 promoter and C-terminal myc-tag entry vectors using the method described above ...
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bioRxiv - Cell Biology 2021Quote: ... ME differentiation was induced by threating PS differentiated cultures for 2 days with BMP4 (R&D, ref. 314-BP, 10 ng/mL) in ME differentiation medium (RPMI 1640, 2% B27 without Insulin (Gibco, ref. A1895601), 1% Penicillin/Streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... SKMEL28 and WM115 melanoma cells plus/minus AR-silencing with two different lentiviruses versus empty vector control were assessed by ClariomTM D GeneChip array analysis (Thermo Fisher Scientific). 5 days post-infection ...
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bioRxiv - Plant Biology 2021Quote: ... with CACC at 5’ end) was cloned in pENTR-D-TOPO vector using pENTRTM Directional TOPO® cloning kit (Invitrogen Inc. USA). This fragment was mobilized to pANDA vector (Miki and Shimamoto ...
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bioRxiv - Microbiology 2020Quote: ... lysates from 106 cells were immunoprecipitated using 4 µg anti-SLC35A1 antibody and Protein G magnetic Dynabeads (Thermo Scientific, 10-003-D) overnight at 4C prior to immunoblotting to enhance signal and specificity of signal.
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Plant Biology 2021Quote: Viral open reading frames (ORFs) from the TYLCV (TYLCV-Alm, Accession No. AJ489258) genome were cloned in the pENTRTM/D-TOPO® vector (Thermo Scientific) (for ORF1 ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification was performed using the pENTR clone containing the genomic NAT2UGT73C6 sequence as template and amplicons were directionally cloned into the pENTRTM /D-TOPO vector (Life Technologies, USA).
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bioRxiv - Plant Biology 2021Quote: ... The OmAGAL2 coding sequence without the expected signal peptide (SP) sequence (ΔSP-OmAGAL, amino acids [AA] 47–412) was cloned into the pENTR/D-TOPO vector (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Microbiology 2020Quote: ... sections of liver biopsies were performed as described previously (6) using a pan-genotype probeset (genotype A-D, VF6-20095, Affymetrix, CA. USA). Sections were stained with NBT (Nitroblue tetrazolium ...
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bioRxiv - Cancer Biology 2020Quote: ... or non-targeting RNAi negative control (Scramble, D-001810-10) (all from Dharmacon) were transfected into HCT 116 cells using Lipofectamine®RNAiMax transfection reagent (Invitrogen, #13778075) at a final concentration of 50 nM ...
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bioRxiv - Cancer Biology 2020Quote: LNCaP cells were seeded at a density of 5×105 cells into Nunclon D multi-dishes with poly lysine coating (Thermo Fisher Scientific), with or without transfection ...
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bioRxiv - Neuroscience 2020Quote: ... 3μg of the plasmids was transfected into HEL293FT cells and cultured on a poly-D-Lysine coated dish containing pre-warmed Opti-MEM® medium (ThermoFisher Scientific) with 2% FBS for 72 hrs ...
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bioRxiv - Biophysics 2021Quote: ... Cells were induced with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 hours and harvested by centrifugation (Sorvall LYNX 6000, ThermoFisher Scientific) at 8,000 rpm for 10 minutes ...
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bioRxiv - Physiology 2022Quote: ... of purified RNA were used as a template to synthesize cDNA using oligo-d(T) primers and SuperScript™ III First-Strand Synthesis SuperMix (Invitrogen, 18080400). STK25 was quantified by real-time PCR using Fast SYBR Green mixture (Life Technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... HeLa cells expressing the fluorescence PAR-T sensors were plated on poly-D-Lysine coated live cell imaging chamber slides (Thermo Fisher, 15411) and cultured in FluoroBrite medium (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: ... The 2622 bp product was directionally cloned by ligation into the pENTR/D-TOPO vector (Invitrogen/Thermo Fisher Scientific, Hemel Hempstead, UK). Digestion with EcoRV and NotI generated 2742 and 2435 bands ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then resuspended in aCSF and incubated with DAPI (1:1000 dilution of 1mg/mL DAPI, Thermo Fisher, #62248 or 7-AAD 7-Aminoactinomycin D, A1310, Thermo Fisher) for FACS sorting ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... The nucleoids were subsequently stained for 5 min at room temperature with 7-amino-actinomycin D (1 mg/ml 7-AAD in DMSO, Thermo Fisher Scientific) diluted 1:400 in PBS buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and combined with 50μl 1× D-Luciferin Working Solution supplemented with 1× Firefly Signal Enhancer (Thermo Scientific Pierce Firefly Signal Enhancer; Thermo Scientific 16180). Following a 10 min dark incubation period to allow for signal stabilization ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM of siRNA non-targeting control (siRNA NTC, D-001810-10-05) or siRNA SOX11 (L-017377-01-0005, Dharmacon) were transiently transfected using DharmaFect 2 (Thermo Fisher Scientific) according to the manufacturer's guidelines ...
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bioRxiv - Developmental Biology 2022Quote: ... by using non targeting (D-001810-10-20) or TAZ (L-016083-00-0005) ON-TARGETplus SMARTpools and Lipofectamine RNAimax (Invitrogen 13778-075).
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bioRxiv - Immunology 2022Quote: ... 3 ×105 Aag2 cells were seeded on an 18 mm, #1 thickness, poly-D-lysine coverslip (Neuvitro, Vancouver, WA) in a 12-well plate (Thermo Fisher Scientific) and cultured at 28°C overnight ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Immunology 2022Quote: ... remaining tissue was digested in 30ml Collagenase solution (RPMI 1640 supplemented with 1mM MgCl2, 1mM CaCl2, 5% FBS, and 100 units/ml collagenase D (Gibco, Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... The fragment was subsequently cloned into the pME vector using the pENTR/D-TOPO Cloning kit (Invitrogen, Waltham MA USA, cat# K240020). Colonies were prepared using the Qiaprep Spin Miniprep kit (QiAgen ...
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bioRxiv - Synthetic Biology 2022Quote: Bacterial cells suspended in LB growth medium were deposited onto a microscope coverslip (coated with poly-D-Lysine (A3890401 Thermo Fisher Scientific)) 18 h after induction of protein expression ...
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bioRxiv - Systems Biology 2020Quote: ... Clone cultures were concentrated to 20-fold in M9 buffer and 10 μL was plated onto a well of a 96 well plate containing NGM agar with 2 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG, Fisher Scientific). Plates were dried and stored at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... the PP2-A13promoter::gPP2-A13 fragment was generated from PCR using Col-0 genomic DNA as template and inserted into pENTR/D-TOPO vectors (Invitrogen, cat. # K240020) then transferred into pGWB16 destination vectors using LR recombination(Nakagawa ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from five tissues (12-d seedlings, tassel, silk, pericarp and 20-DAP seeds) with TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cultured neurons were transfected with plasmids (1.5 μg of plasmid DNA per well) at 4 - 5 d in vitro (DIV) using a commercial calcium phosphate transfection kit (Thermo Fisher Scientific) as previously described21,24.