Labshake search
Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... concentrated and washed for 3 min on a C18 reverse phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6 μm particle size ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
-
bioRxiv - Neuroscience 2024Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
-
bioRxiv - Cell Biology 2024Quote: ... The Triton-X permeabilization solution was removed and cells were washed 3×2 minutes with DPBS at RT before a blocking solution (1% BSA (Fisher Scientific, BP1600-100) was added to cells for one hour at RT with rocking ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Females were then primed with subcutaneous administration of estradiol benzoate (17-β-Estradiol-3-Benzoate, Fisher Scientific, 2 μg dissolved in sesame oil) for the 3 days preceding any days on which they were recorded.
-
bioRxiv - Evolutionary Biology 2024Quote: ... or 150 heads or tails 3 hours post amputation (hpa) were transferred to a 2 mL tissue grinder (Fisher Scientific, 50-212-708) and washed with artificial seawater ...
-
bioRxiv - Systems Biology 2024Quote: ... Samples were loaded onto a pre-column (Acclaim PepMap 100, 75 µm x 2 cm, C18, 3 µm and 100 Å particles, Thermo Fisher Scientific) before being separated over a 25-cm IonOpticks Aurora Series column (IonOpticks) ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were washed with PBST 3×2 minutes then counterstained with a fluorophore conjugated variation of wheat germ agglutin (Invitrogen; W11261; 10ug/mL), phalloidin (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: Samples were loaded onto Acclaim PepMap 100 C18 column (75 μm × 2 cm, 3 μm particle size and 100 Å pore size; #164946, Thermo Fisher Scientific) and separated using nano-capillary C18 column ...
-
bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
-
bioRxiv - Immunology 2022Quote: ... The cell suspension was centrifuged at 500 g for 5 min at 4°C and the cell pellet was incubated with ACK lysing buffer (Thermo Fisher Scientific, A1049201) for 5 minutes to lyse the red cells ...
-
bioRxiv - Molecular Biology 2022Quote: To control efficiency of the enzymatic treatment 5 μg of the enzyme-treated products or controls were run on a NuPage™ 4-12 % Bis-Tris gel (Invitrogen, Carlsbad, USA) at 100 V for 60 minutes using the EI9001-XCELL II Mini Cell (Novex ...
-
bioRxiv - Microbiology 2021Quote: ... murine PBMCs were stimulated for 4 hrs with spike (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies, California, USA) for a further 10-12 hrs ...
-
bioRxiv - Biochemistry 2021Quote: Protein samples were heated for 5 min at 95 °C and separated by electrophoresis in NuPAGE™ 4-12 % gels (ThermoFisher, Cat# NP0329BOX). RNA samples were heated for 2.5 min at 95 °C and separated by electrophoresis in denaturing 9 % acrylamide (19:1 ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated at room temperature for 4-5 h in Triton PBS containing Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:1000; A-21207, Thermo Fisher Scientific, RRID:AB_141637). After binding of streptavidin/antibodies ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were seeded 4–5 × 104 cells per well in 8-well Lab-Tek II chambered cover glass slides (Nunc, Rochester, NY, USA) and incubated overnight ...
-
bioRxiv - Biophysics 2020Quote: ... 20 μL of protein solution was incubated with 4 μL of LDS sample buffer and incubated at 100°C for 5 mins before 10 μL was loaded on a 4-12% bis-tris gel (NuPAGE™, Thermo Fisher Scientific). The gel was either stained with Coomassie blue or transferred to a 0.22 μm polyvinylidene fluoride (PVDF ...
-
bioRxiv - Systems Biology 2022Quote: ... and the resulting supernatant (100 ng/ml) was incubated for 4 h at 37°C together with 5 mM glutamine (Gibco, Cat# 25030-081), 1 mM phosphoribosyl pyrophosphate (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... The in vitro transcription reaction for the 5’ cap driven Fluc reporter included a 1:4 ratio of Invitrogen™ Anti-Reverse Cap Analog (Fisher Scientific AM8045) and GTP solution (0.4 µl cap analog to 1.6 µl of GTP solution) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cell suspensions were placed in clean cytospin cones and spun at 600g for 5 min on an Epredia Cytospin 4 (Fisher Scientific Cat#A78300003) to charged glass slides ...
-
bioRxiv - Microbiology 2021Quote: ... murine PBMCs were stimulated for 4 hrs with SpK (5 μg/mL) and then supplemented with Protein Transport Inhibitor cocktail (Life Technologies, California, USA) for a further 10-12 hrs ...
-
bioRxiv - Molecular Biology 2020Quote: ... To clear the homogenate from debris and larger worm fragments it was centrifuged at 800 g for 5 minutes at 4°C (Heraeus Fresco 21, Thermo Scientific, Langenselbold, Germany) and the supernatant collected ...
-
bioRxiv - Microbiology 2020Quote: ... cells were washed thrice with sterile PBS and 200 μL of cell suspension were cytospined for 5 min (Shandon Cytospin 4, Thermo Scientific, Cheshire, UK). The identification of infected DPSCs was carried by Gimenez staining.
-
bioRxiv - Immunology 2021Quote: ... Antibodies were harvested from cell supernatants at 4 to 5 days post transfection and purified using CaptureSelect™ LC-lambda (mouse) Affinity Matrix (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2021Quote: 3D caecaloids grown in expansion medium for 4-5 days after passaging were dissociated into single cells by TrypLE Express (Gibco Thermo Fisher Scientific) digestion ...
-
bioRxiv - Cancer Biology 2020Quote: ... Dissociated tumor cells from Lcre;NICD1 animals treated with IMR-1 or vehicle were resuspended in 0.2 ml cold phosphate-buffered saline (PBS) and spun onto a slide at 800 g for 5 min using a Shandon Cytospin 4 Cytocentrifuge (Thermo Scientific, Waltham, MA).
-
bioRxiv - Physiology 2023Quote: ... Proteins were denatured at 95°C for 5 minutes and run on a Bolt™ 4-12% Bis-Tris polyacrylamide gel (Thermo Scientific #NW04125BOX). Proteins were transferred to a nitrocellulose membrane (Invitrogen #PB3310 ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol, 50 mM maltose) and were concentrated using an Amicon Ultra Centrifugal Filter Unit (50 kDa, 4 ml) (Fisher Scientific, EMD Millipore). Proteins were further purified by gel filtration chromatography (Superdex-200 ...
-
bioRxiv - Systems Biology 2023Quote: Labeled blots were washed 4 x 5 min each then incubated in SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific™ #34577) at room temperature for 3 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... Differentiation was induced at ∼85% confluency by switching to media containing 4:1 DMEM/M199 supplemented with 5% normal horse serum (Invitrogen; cat #: 26050-088). Following seven days of differentiation cells were treated with either ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction tubes were centrifuged for 5 min at 4 °C and 23000 rpm (approx. 49000 x g, Heraeus Biofuge Stratos, rotor 3331, Thermo Scientific, Waltham, USA). In the next step ...
-
bioRxiv - Neuroscience 2023Quote: ... before cells were spun down at 200xg for 5 min and fixed in 4% PFA for 10 min followed by permeabilization in perm buffer (Invitrogen, #00-8333-56) with 10 μg/mL Hoechst (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 μL ribodepleted RNA was mixed with 4 μL 5× TGIRT buffer (2.25 M NaCl, 25 mM MgCl2, 100 mM Tris-HCl, pH 7.5; Ambion RNase-free Buffer Kit AM9010), and 1 μL random hexamer primers (xGen) ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were incubated over-night at 4°C with the following primary antibodies: mouse anti-claudin-5 (Invitrogen, USA, 1:100 dilution) and rabbit anti-occludin (Invitrogen ...
-
bioRxiv - Plant Biology 2024Quote: ... annealing at 52ºC for 5 s and extension at 60ºC for 4 min using a GeneAmp® PCR System 9700 thermocycler (Applied Biosystems, Warrington, UK). Samples were Sanger-sequenced using a 3730 Series Genetic Analyzer Thermo Fisher Scientific UK Ltd (Loughborough) ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS and then stained for 15min at 4 °C in Flow buffer (PBS with 5% cosmic calf serum) containing the following antibodies: CD11b-PE (Invitrogen, 12-0112-82), CD45-APC (Biolegend ...
-
bioRxiv - Immunology 2022Quote: ... at 300 x g for 5 min at 4°C and resuspended in 50 μl of unlabeled anti-FCGR3/CD16-FCGR2B/CD32 Ab (Invitrogen, 14-0161-86) 10 μg/ml in FACS-buffer (PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... and 0.005 % BPB) and incubated 5 min at 100 ºC followed by electrophoresis on Novex™ 4-20% Tris-Glycine gradient gels (Invitrogen, WXP42020BOX; WXP42026BOX). Proteins were transferred onto 0.2 µm nitrocellulose membranes (Pall Laboratory ...
-
Optimisation of immunocytochemistry methodology for the detection of endogenous eIF2B localised focibioRxiv - Cell Biology 2022Quote: ... Cells were then washed with PBST 4 times for 5 min and mounted using ProLong™ Gold Antifade Mountant with DAPI 220 (Invitrogen, Thermo Fisher Scientific). Cells were viewed on a Zeiss LSM 800 confocal microscope.
-
bioRxiv - Neuroscience 2024Quote: ... Both were punched out together using a Militex biopsy Punch (4 mm, 15110-40-X) which was washed prior to sorting with either 700 µL RNAsecure (1:5; Thermo Fisher Scientific, AM7006) or Millipore Protease inhibitor cocktail III (1:750 ...
-
bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135 ...
-
bioRxiv - Bioengineering 2023Quote: ... 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC; 22980) was purchased from Thermofisher Scientific (MA) ...
-
bioRxiv - Developmental Biology 2020Quote: Skin tissues collected from newborn Foxn1−/− (Cby.Cg-Foxn1
/cmdb) and Foxn1+/+ (C57BL/6; B6) mice were incubated in dispase (6 U/ml; Life Technologies) overnight at 4°C ... -
bioRxiv - Biochemistry 2021Quote: ... were incubated without ligand or with EGF (6 μM) or EREG (6 μM) in buffer A containing disuccinimidyl suberate (DSS) at 62.5 μM (ThermoFisher Scientific) for 30 min at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
-
bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transiently transfected in 6-well plates with 6 individual IFNLR1-gRNA plasmids using Lipofectamine 3000 (Life Technologies). The cells were harvested 4 days post-transfection and subjected to fluorescence-activated cell sorting (FACS ...
-
bioRxiv - Cancer Biology 2022Quote: ... and ran on QuantStudioTM 6 Flex Real-Time PCR System using QuantStudioTM 6 and 7 Flex Real-Time PCR software v1.0 (Applied Biosystems). Relative gene expression levels were quantified using β-actin or human TBP as housekeeping genes ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pro-inflammatory cytokine (IL-8) and (IL-6) release was determined using the IL-6 and IL-8 ELISA kit (Invitrogen) according to the manufacturer’s instructions (ThermoFisher).