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Citations for Thermo Fisher :
6451 - 6500 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells; ATCC CCL-244) were cultured in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolates (Human/NL/Lelystad/2020) were propagated in Vero E6 cells (ATCC) in Eagle’s minimal essential medium (Gibco) supplemented with 1% L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... Asexual parasites were cultured in human blood (UK National Blood Transfusion Service) and RPMI 1640 medium containing 0.5% w/v AlbumaxII (Invitrogen) at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgM and IgG conjugates provided within the kit were replaced with anti-mouse IgM-HRP-labeled (Invitrogen) and anti-mouse IgG-HRP-labeled (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Immunology 2020Quote: ... human embryonic kidney cells (HEK) 293T were grown in a T75 flask with Dulbecco’s modified eagle medium (DMEM) (Gibco) + 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... gondii tachyzoites were maintained by growth on monolayers of human foreskin fibroblasts (HFF) in Dulbecco’s Modified Eagles Medium (DMEM) containing 10% Cosmic Calf Serum (GIBCO), 2 mM Glutamine ...
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bioRxiv - Immunology 2020Quote: ... the fixed sample was incubated with 1:500 dilution of goat anti-human coupled to Alexa 633 fluorophore (Invitrogen) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2021Quote: Cytokines in AqH samples were quantified using a ProcartaPlex Human Cytokine-Panel 1B (Thermo Fisher Scientific, Waltham Massachusetts, USA) that quantifies ...
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bioRxiv - Immunology 2021Quote: Human CD4 T cells were MACS-sorted from PBMC and subsequently pre-activated with α-CD3/CD28 Dynabeads (Thermofisher) at a bead-to-cell ratio of 1:1 for 48 hours ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and 50 μL of a secondary Alexa Fluor 647 goat anti-human antibody (Life Technologies A21455) (1:500 in PBS with 0.1% (w/v ...
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bioRxiv - Immunology 2020Quote: A CRISPR plasmid targeting human Hk2 was generated using the GeneArt® CRISPR Nuclease Vector Kit from Thermo Fisher. Caco-2 cells were purchased from DSMZ (ACC-169 ...
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bioRxiv - Genomics 2021Quote: ... Naive and memory T cells were then stimulated with anti-CD3/anti-CD28 human T-Activator Dynabeads® (Invitrogen) at a 1:2 beads-to-cells ratio ...
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bioRxiv - Genomics 2021Quote: ... RNA for gene expression analysis from primary human pseudoislets was extracted using the PicoPure RNA isolation kit (Life Technologies) and cDNA was synthesized using the Maxima first strand cDNA synthesis kit (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: Human iPSCs from WTC11 and NCRM5 backgrounds3 were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific; Cat. No. A3349401) in plates or dishes coated with Growth Factor Reduced ...
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bioRxiv - Genetics 2021Quote: ... were genotyped using the Affymetrix Genome-Wide Human SNP Array 5.0 (SNP5) chip (Affymetrix Inc., Santa Clara, CA, USA). For the Athero-Express Genomics Study 2 (AEGS2) ...
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bioRxiv - Genomics 2020Quote: The cells The human embryonic kidney 293T (HEK293T) cell were cultured in DMEM containing 10% FBS (Thermo Fisher Scientific), 100 U/ml penicillin (A603460 ...
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bioRxiv - Microbiology 2020Quote: ... and human umbilical vein endothelial HUV-EC-C cells (ATCC; CRL-1730) in RPMI 1640 medium (Gibco; #11875-093) containing 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
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bioRxiv - Immunology 2022Quote: ... Human THP-1 monocytes (ATCC, TIB-202) were grown in RPMI 1640 Medium with ATCC modification (Thermo Fisher Scientific) supplemented with 10% FBS and 50 μM 2-mercaptoethanol (Millipore Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: Human embryonic genital ridge or mesonephros from a week 7.0 male embryo were collected in dissection medium (DMEM (Gibco), 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... When HIV was reactivated using Dynabeads Human T activator CD3/CD28 for T cell expansion and activation (ThermoFisher Scientific), beads were magnetically removed the next day ...
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bioRxiv - Cell Biology 2022Quote: ... human embryonic kidney 293T cells (HEK; CRL-3216; ATCC; RRID: CVCL_0063) were cultured in DMEM media (12430062; Gibco™) with 10% FBS (098-150 ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies [Alexa 647-goat anti-mouse or Alexa 647 Goat anti-Human IgG (H+L) Cross-Adsorbed (Invitrogen)] at 1:1000 dilution were then incubated for 60 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: Large-scale production of bio-inactive human N-SLIT2ΔD2 was performed by transfecting HEK293F cells with human N-SLIT2ΔD2 cDNA (1 ug/mL) using PEI reagent (Polyethyleneimine, linear, M.W. 25,000, Thermo Fisher Scientific) (19) ...
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bioRxiv - Immunology 2022Quote: ... Wells were then incubated with a secondary goat anti-human IgG labeled with horseradish peroxidase (HRP) (1:5000 Invitrogen) TMB substrate (SIGMA) ...
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bioRxiv - Immunology 2022Quote: Supernatants were collected 3 days post Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco) stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel ...
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bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: Human THP-1 cells were cultured in RPMI-1640 medium containing 2 mM L-glutamine (Gibco; Grand Island, NY), 10% FBS ...
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bioRxiv - Microbiology 2022Quote: Human HEK-293T adherent cells were seeded at 37°C with 5% CO2 in DMEM + GlutaMAX™ (Gibco, Germany) containing 10% heat inactivated fetal calf serum (dFCS ...
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bioRxiv - Microbiology 2022Quote: ... with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen). Biotinylated lectins were precomplexed (on ice ...
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bioRxiv - Molecular Biology 2022Quote: CHO-K1 cells stably expressing human RXFP4 (hRXFP4-CHO) or RXFP3 (hRXFP3-CHO) were maintained in DMEM/F12 (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... slides were incubated for 1 hr with goat anti-human 555 (Thermo Fisher Scientific, A-21433; 1:1000 dilution) and goat anti-rabbit 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Fresh-frozen human postmortem brain tissues were sectioned at 16-25 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes on dry ice and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Molecular Biology 2023Quote: ... The MS/MS spectra were searched against the target-decoy Human RefSeq database (release Jan. 21, 2020, containing 80,872 entries) in Proteome Discoverer 2.1 interface (Thermo Fisher) with Mascot algorithm (Mascot 2.4 ...
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bioRxiv - Microbiology 2023Quote: ... and cytokine concentrations were quantified with the Human Cytokine 16-Plex Protein Standard (Thermo Fisher Scientific, Waltham, MA, USA). For quantification of BPI in human sera ...
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bioRxiv - Microbiology 2022Quote: ... acquired MS/MS spectra were searched against a human uniprot protein database in Proteome Discoverer 3.2 (Thermo Fisher Scientific) using Label free Quantification with Minora feature detection ...
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bioRxiv - Neuroscience 2022Quote: ... conditioned neuronal media was diluted and handled according to the protocol of the Human Aβ42 Ultrasensitive ELISA Kit (Invitrogen) followed by colorimetric readout at 450 nm.
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bioRxiv - Molecular Biology 2022Quote: Human AAA/ control tissue samples were mounted on poly-L-lysine pre-coated SuperFrost Plus slides (Thermo Fisher Scientific). Human OCT-embedded frozen tissue was cut into 8μm thick slides ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (human)-Alexa Fluor 594 (T13343) and Wheat Germ Agglutinin (WGA)-Alexa Fluor 594 (W11262) were obtained from ThermoFisher Scientific (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by washing and addition of 100 µL/well of goat anti-human IgG-HRP conjugate (Invitrogen, 62-8420) at 1:500 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was analyzed for gene expression changes using a microarray analysis (Clariom S Human HT, Thermo Fisher Scientific). Each replicate (100ng of total RNA ...
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bioRxiv - Biophysics 2024Quote: The human melanoma cell line MV3 [32] was cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, 11504496) containing L-Glutamine and Sodium Pyruvate ...
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bioRxiv - Biochemistry 2024Quote: ... followed by staining with Alexa Fluor 647-conjugated Goat anti-Human antibody (Thermo Fisher Scientific, A-21445, 1:500).
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... a solution of 10-3 mg/ml PE- conjugated anti-human CD62E (clone P2H3, Thermofisher Scientific, #12-0627-42) has been introduced in the chips to stain the endothelium for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... The levels of soluble and insoluble human Aβ40 and Aβ42 in these extracts were quantified using ELISA kits (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... falciparum erythrocytic stages were cultured in human erythrocytes (National Blood Transfusion Service, UK) and RPMI 1640 medium (Life Technologies) supplemented with 0.5% Albumax type II (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... falciparum (3D7, Dd2, and HB3) parasites were cultured in 3-5% human O+ RBCs in RPMI-1640 (Gibco, 110875093) complete medium containing 0.5% Albumax-II (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of HAs was performed with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen) and incubated on ice for 20 min ...