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Citations for Thermo Fisher :
6251 - 6300 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... cells were stained with Alexa Fluor 647-conjugated goat anti-human antibody (Invitrogen; cat# A-21445, 1/500). The control VHH Fc (R3VQFc ...
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bioRxiv - Biophysics 2024Quote: Human embryonic kidney 293T cells (HEK293T) 50 were maintained in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in a 1:1:1:1 ratio of human receptor:Gαq-Rluc8:β3:GFP2-γ9 DNA constructs prepared in Opti-MEM (Invitrogen) using a 3:1 ratio of TransIT-2020 (Mirus Bio ...
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bioRxiv - Neuroscience 2024Quote: Human Embryonic Kidney 293T (HEK293T) and HeLa cells were cultured in DMEM supplemented with 10% FBS (Life Technologies). 1 % trypsin was employed to detach cells and split them ...
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bioRxiv - Immunology 2024Quote: ... incubated for 1h with either horseradish peroxidase (HRP)-conjugated goat anti-human IgM or IgG (Invitrogen, Camarillo, CA), washed again ...
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bioRxiv - Biophysics 2024Quote: Human cervical adenocarcinoma cells (HeLa) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with GlutaMAX™ (Gibco, ThermoFisher) supplemented with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Systems Biology 2024Quote: 150 human islets were hand-picked on the day of distribution and placed in 1ml of Trizol (Invitrogen), vortexed and stored at −80°C until shipping to Oxford or Stanford for further processing ...
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bioRxiv - Microbiology 2024Quote: ... and anti-human IgG conjugated to Alexa 488 and Alexa 555 were purchased from Invitrogen (Thermo Fisher Scientific). The Golgi (RCAS1 ...
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bioRxiv - Microbiology 2024Quote: ... Gametocyte cultures were seeded from asexual blood stage cultures at 2% parasitaemia and 4% haematocrit in gametocyte medium (RPMI-1640 with 5% human AB+ serum, 0.5% Albumax, 3.7% 100xHT supplement (ThermoFisher), 30 mg/ml L-glutamine ...
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bioRxiv - Molecular Biology 2024Quote: Telomerase-immortalized human myometrial (hTERT-HM) cells (52) were cultured in phenol-red free DMEM/F12 (ThermoFisher Scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2024Quote: ... human and primary mouse myotubes were maintained in a mixture of DMEM/F-12 (Gibco: Waltham, MA, USA) containing 20% FBS (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with with 5% human platelet lysate (hPL, Stemmatters, Portugal) and 1% Penicillin-Streptomycin (P/S, Invitrogen USA). ASCs were maintained at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: Human iPSCs from the WTC11 background were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific; Cat. No. A3349401). Human iPSCs from the GIH6C1 background were cultured in mTeSR Plus medium (StemCell Technologies ...
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bioRxiv - Cell Biology 2020Quote: Plasmodium falciparum parasites were grown in human erythrocytes (Stanford Blood Center) at 2% hematocrit in RPMI 1640 media (Gibco) supplemented with 0.25% Albumax II (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: The HaCaT cell line (Human adult low Calcium high Temperature) were grown in Dulbecco’s Modified Eagle Media (DMEM, Invitrogen) supplemented with 2 mM essential amino acids (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (1500 nM) ...
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bioRxiv - Genetics 2021Quote: ... Fetal cortex samples were acquired from the MRC-Wellcome Trust Human Developmental Biology Resource (HDBR) (http://www.hdbr.org/) in Hibernate-E media (ThermoFisher Scientific). All samples were obtained through elective terminations of pregnancy ...
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bioRxiv - Microbiology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors by performing a Ficoll-Paque gradient (Thermo Fisher) on whole blood ...
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bioRxiv - Immunology 2020Quote: ... Samples were thawed on ice and prepared according to the manufacturer’s instructions using a custom-made 33-plex human cytokine panel (Procartaplex ThermoFisher). In brief ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: The screening was performed with a genome-wide siRNA library (Ambion SilencerR Human Genome siRNA library v3, Thermo Fisher), composed of siRNAs targeting approximately 21000 genes arrayed on 384-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... kidney) and HEK293T (Human embryonic kidney) cell lines were cultivated in Dulbecco’s modified Eagle (DMEM) (Thermo Scientific #12100-046) medium ...
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bioRxiv - Microbiology 2021Quote: Immortalized human vaginal epithelial cells (VK2/E6E7, ATCC CRL-2616) were cultured in keratinocyte serum-free medium (KSFM) (Gibco) with 0.5 ng/mL human recombinant epidermal growth factor and 0.05 mg/mL bovine pituitary extract ...
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bioRxiv - Neuroscience 2022Quote: Human iPSCs (male WTC11 background, PMID 24509632) were cultured in StemFlex™ Basal Medium (Gibco; Cat. No. A33493-01) on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Human female osteosarcoma U2OS and breast cancer MCF7 cells were cultured in DMEM + Glutamax (Thermo Fisher Scientific, 31966-047) supplemented with 10% FCS and 1% Penicillin/Streptomycin ...
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bioRxiv - Neuroscience 2021Quote: Primary hippocampal neurons were transfected with 1.5 μg human α-synuclein-GFP plasmid DNA [48] using lipofectamine-LTX (Thermofisher) on DIV6 and imaged on DIV10 using an inverted ZEISS Z1 Cell Observer fitted with a Colibri2 cool LED 5-channel fast wavelength switching system ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA expression analysis was performed with Affymetrix Human ClariomS® microarray using the WT Plus Reagent kit (ThermoFisher Scientific). As starting material ...
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bioRxiv - Immunology 2021Quote: ... The nanoparticles were resuspended in DPBS++ containing 1μM biotin-labeled anti-human kappa light chain (Igκ) (CaptureSelect; Thermo Fisher), and incubated 20min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... mouse fibroblast NIH-3T3 L929 cells and human embryonic kidney HEK 293 cells were grown in DMEM medium (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with sterile-filtered 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... MDCK-CMAH cells were prepared by transfection of the human CMAH gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Clones with Neu5Gc expression were determined by HPLC analysis as previously described (63) ...
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bioRxiv - Biochemistry 2019Quote: ... Human WT (GM03652), NPC1 (GM03123), and NPC2 (GM18455) fibroblasts (Coriell Institute, Camden, NJ) were maintained in DMEM media (Invitrogen) supplemented with 15% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Immunology 2019Quote: ... and human bronchial carcinoma Calu3 epithelial cells (ATCC_HTB-55) were grown in 10% FCS in alpha MEM media (Gibco). Human alveolar epithelial carcinoma A549 epithelial cells (ATCC_CCL-185 ...
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bioRxiv - Microbiology 2019Quote: ... and C were selected and designed using MEGA6 software [30] and evaluated by the Thermofisher custom genomic TaqMan assay and design pipeline for non-human templates (https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping) and analyzed using the ABIprism 7900HT (Applied Biosystems): SpaP SNP2137-2139 [GT/CG] ...
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bioRxiv - Genomics 2019Quote: ... The mouse and human RNA samples were globin-depleted using the species-specific Ambion® GLOBINclear kit (Ambion, Thermofisher) and the RNA was quantified using a NanoDrop One spectrophotometer (Thermofisher).
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bioRxiv - Genomics 2019Quote: ... The mouse and human RNA samples were globin-depleted using the species-specific Ambion® GLOBINclear kit (Ambion, Thermofisher) and the RNA was quantified using a NanoDrop One spectrophotometer (Thermofisher).
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bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
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bioRxiv - Immunology 2020Quote: ... The human monocytic THP-1 cell line was maintained in RPMI-1640 (made inhouse, RPMI 1640 powder (Life Technologies), 23.8mM Sodium Bicarbonate (NaHCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... Data were processed by database searching against Human Uniprot Proteome database using Proteome Discoverer 2.3 software (Thermo Fisher Scientific). Precursor and fragment mass tolerance were set at 10 ppm and 0.6 Da respectively ...
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bioRxiv - Genetics 2019Quote: ... to distinguish recent and distant relatedness in the sample using markers from the Genome-Wide Human SNP 6.0 array (Affymetrix). The percent of total variation in height and BMI was estimated using the coefficient of determination (R2 ...
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bioRxiv - Developmental Biology 2020Quote: Human iPS cells (iPSCs, ATCC-DYS0100) were maintained in self renewal on a layer of geltrex (Gibco, A14133-01), in E8 Basal Medium (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Unlabeled and ferumoxytol-labeled human MSCs were tested for tri-lineage differentiation potential using StemPro differentiation kits (Life technologies) for adipogeneic ...
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bioRxiv - Genomics 2019Quote: ... The HUES9 human embryonic stem cell line was grown on tissue culture plates coated wth Geltrex (Thermo Fisher Scientific) in mTeSR1 media (StemCell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Data were processed by database searching against Human Uniprot Proteome database using Proteome Discoverer 2.2 software (Thermo Fisher Scientific). Precursor and fragment mass tolerance were set at 7 ppm and 0.6 Da respectively ...
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bioRxiv - Developmental Biology 2019Quote: DNA sequences corresponding to the human Amyloidβ1-42 amino acid sequence were synthesized (IDT) into a Gateway technology (Invitrogen) entry vector ...
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bioRxiv - Microbiology 2019Quote: ... and human glioblastoma cells (U-87MG, ATCC no.: HTB-14) were propagated in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies). Chinese hamster ovary cells (CHO-K1 ...
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bioRxiv - Cancer Biology 2020Quote: ... The labeled samples were then hybridized to Affymetrix Human Genome U133 Plus 2.0 Array GeneChips (ThermoFisher Scientific, Waltham MA) and scanned Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA from sorted CXCR3+ or CXCR3− SUM-LM1 cells was analyzed using Affymetrix Human U133 Plus 2.0 microarrays after cDNA preamplification using the 3’IVT Pico Reagent Kit (Affymetrix). To generate the CXCR3 signature ...
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bioRxiv - Cancer Biology 2019Quote: The immortalised non-tumorigenic human epithelial cell line MCF12A (ATCC, CRL-10782),90 were cultured in DMEM/F12 (Gibco) supplemented with 5% horse serum (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... Swabs were screened for common human respiratory pathogens using a customized TaqMan Array Card® (ThermoFisher, Waltham, MA, USA), which included influenza A and B viruses ...
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bioRxiv - Immunology 2021Quote: ... NAFs/CAFs ± SI were added to wells of lymphocytes at a concentration of 1×104 cells/100μl (ratio of 1:10 NAFs/CAFs:lymphocytes) of human complete MSC medium (RPMI 1640 medium (ThermoFisher Scientific) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2020Quote: Human embryonic kidney (HEK)-293 cells (ATCC, CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (Gibco, 11965-092) supplemented with 10% (v/v ...