Labshake search
Citations for Thermo Fisher :
6351 - 6400 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: The total amount of soluble tau in S1 fractions was determined by using an ELISA kit (human Tau, Invitrogen), according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... and human T lymphocytes, Jurkat J16 (Adult lymphoblastic leukemia ACC-282, DSMZ) were cultured in RPMI-1640 (Thermo Fisher) supplemented with 10% FCS ...
-
bioRxiv - Microbiology 2022Quote: ... and human foreskin fibroblast cells immortalized with human telomerase reverse transcriptase (HFF-hTERT cells)96 were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher) supplemented with 10% (v/v ...
-
bioRxiv - Molecular Biology 2022Quote: Human Embryonic Kidney (HEK293T) cells were cultured in high glucose DMEM with 10% FBS and 1% Penicillin/Streptomycin (Gibco) in a 37°C incubator with 5% CO2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... falciparum erythrocytic stages were cultured in human erythrocytes (National Blood Transfusion Service, UK) and RPMI 1640 medium (Life Technologies) supplemented with 0.5% Albumax type II (Gibco) ...
-
bioRxiv - Synthetic Biology 2022Quote: Codon-optimized DNAs encoding the variable library parts were codon optimized for expression in human cells using ThermoFisher GeneArt’s website tool and synthesized by ThermoFisher GeneArt ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... mice were bled and total IgG (mouse and human) was pulled down using protein A beads (ThermoFisher, Catalog # 20333). An in–house sandwich ELISA was used to measure human IgG (control IgG or MS-Hu6 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: 1 mL of human plasma was incubated with 0.5 mL of Invitrogen total exosome isolation reagent (Thermo Fisher Scientific) overnight at 4°C and the day after was centrifuged at 10,000 x g for 1h at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: Human Embryonic Kidney Expi293 cells were grown to a density of approximately 2×106 in Expi293 medium (Thermo Scientific). Following stress induction upon addition of 100 mM (final concentration ...
-
bioRxiv - Bioengineering 2022Quote: cDNA libraries were constructed using Ion Ampliseq Transcriptome Human Gene Expression Kit from Thermo Fisher (MA, USA, Cat# A26325) according to manufacturer’s recommended protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... Rat ASC were grown in the same medium than human ASC supplemented with 10% of fetal calf serum (Thermofisher). To manufacture the ASC-enriched gelatin patch ...
-
bioRxiv - Cell Biology 2022Quote: Human mammary epithelial MCF-10A cells were cultured in basal Dulbecco’s Modified Eagle Medium (DMEM) (Gibco by Life Technologies) with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Cell Biology 2022Quote: Human mammary epithelial MCF-10A cells were cultured in basal Dulbecco’s Modified Eagle Medium (DMEM) (Gibco by Life Technologies) with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... All media for human cell lines were supplemented with 100 U/mL penicillin and 100 µg/mL streptomycin (Gibco) and FBS (Peak Serum) ...
-
bioRxiv - Neuroscience 2021Quote: ... PLA on human post-mortem paraffin-embedded tissue was performed on sections mounted on superfrost plus slides (Thermo Scientific). 6 μm-thick human caudate-putamen sections were deparaffinized in xylene ...
-
bioRxiv - Molecular Biology 2021Quote: Affymetrix Human Exon 1.0 ST arrays were hybridized by GenoSplice technology (www.genosplice.com) according the Ambion WT protocol (Life Technologies, France) and Affymetrix (Santa Clara ...
-
bioRxiv - Microbiology 2020Quote: ... Meso Scale Discovery (MSD)) were coated with mouse anti-human IgG antibody (CH2 domain, cat# MA5-16929, ThermoFisher Scientific) at 2 μg/mL in 1X PBS (50μL/well) ...
-
bioRxiv - Microbiology 2021Quote: Human foreskin fibroblasts (HFFs) were seeded in 96 wells plates and grown to confluency in DMEM medium (ThermoFisher 11960044) at 37°C in 5% CO (26) ...
-
bioRxiv - Genetics 2021Quote: ... 10 μg of total RNA was depleted of rRNAs by using the Human/Mouse RiboMinus Transcriptome Isolation Kit (Invitrogen). Ribosomal RNA depletion was confirmed by an Agilent 2100 Bioanalyzer (Agilent) ...
-
bioRxiv - Cell Biology 2021Quote: ... The slides were washed and incubated for 2 h with secondary antibodies (Alexa 488 anti-Human IgG Thermo Fisher - #A11013 and Alexa Fluor 555 Anti-Mouse IgG #A21422) ...
-
bioRxiv - Neuroscience 2021Quote: Human ESCs and iPSCs were maintained on plates coated with geltrex (life technologies, A1413301) in StemFlex media (Gibco, A3349401) and passaged with accutase (Gibco ...
-
bioRxiv - Microbiology 2020Quote: ... ATCC CCL-81) and human embryonic kidney cells (HEK293T; ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle media (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
-
bioRxiv - Microbiology 2020Quote: ... ATCC CRL-1586) and HEK-293T (human embryonic kidney cells) cells were maintained in Dulbecco’s Modified Eagle Medium (Gibco). All media were supplemented with 10% fetal bovine serum (Hyclone) ...
-
bioRxiv - Microbiology 2020Quote: ... goat anti-human IgG labeled with FITC (Chemicon) or goat anti-rabbit IgG labeled with Alexa 488 (Thermo Scientific) was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI ...
-
Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: cDNAs encoding human full-length of CREB3L3 (NM_032607.2) and green fluorescent protein were cloned into pENTR4 vectors (Life Technologies). In addition ...
-
bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
-
bioRxiv - Neuroscience 2020Quote: The quantitation of soluble human Aβ1-42 in hippocampus extracts was determined with a sandwich antibody ELISA kit (Invitrogen), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
-
bioRxiv - Microbiology 2021Quote: Human adenocarcinomic lung epithelial (Calu-3) cells (ATCC, HTB-55) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO) supplemented with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Neuroscience 2022Quote: ... APOE levels were similarly processed and quantified from iMGL conditioned media using Apolipoprotein E Human ELISA kit (Invitrogen; #EHAPOE).
-
bioRxiv - Neuroscience 2022Quote: ... Caco-2 and human skin fibroblast cells were grown in Dulbecco’s modified Eagles medium (DMEM) with Glutamax (Life Technologies) and supplemented with 10% fetal bovine serum ...
-
bioRxiv - Microbiology 2022Quote: ... Human vaginal epithelium cells (hVECs, VK2/E6E7) were cultivated in Keratinocyte-Serum Free medium (Thermo Fisher Scientific, Massachusetts, USA) at 37°C in 5% CO2 as the suggestion by ATCC.
-
bioRxiv - Neuroscience 2022Quote: A 448 nt fragment of human SYN1 promoter was PCR amplified with Phusion high fidelity DNA polymerase (Thermo Scientific) and the following primers ...
-
bioRxiv - Immunology 2022Quote: ... The detection antibody used was horseradish peroxidase (HRP)–conjugated goat anti-human IgG (H+L) (ThermoFisher, cat# SA5-10283) at a 1:2000 dilution ...
-
bioRxiv - Immunology 2022Quote: Fn purified from human plasma (sigma) or monoclonal antibodies were fluorescently conjugated using AlexaFluor 488 and 647 Ester (Invitrogen). The conjugation and purification were done according to the manufacturer’s guidelines ...
-
bioRxiv - Immunology 2022Quote: ... Human monocyte-derived macrophages (MDMs) were derived from CD14+ peripheral blood monocytes by culturing cells in RPMI/1640 (Gibco) media containing 10% heat-inactivated human AB serum (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: Human U937 macrophages (ATCC, Cat. No. CRL-1593.2™) were maintained in RPMI (Thermo Fisher Scientific, Cat. No. 11875093) supplemented with 10% FBS (RMBIO ...
-
bioRxiv - Cell Biology 2022Quote: The cDNA encoding full-length human CLASP2a was purchased from Dharmacon (Accession: BC140778.1) and subcloned into: (i) a pFastBacHT vector (Invitrogen) containing an N-terminal 6xHis-tag and ...
-
bioRxiv - Molecular Biology 2019Quote: Human MZF1 cDNA was subcloned from pOTB-MZF1 into pcDNA3-Flag vector via TOPO directional cloning (Thermo Fisher Scientific) and designated pcDNA3/Flag-MZF1 ...
-
bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
-
bioRxiv - Immunology 2019Quote: ... Plates were washed 6x 300μl PBST and developed with an anti-human Fc specific secondary antibody (Invitrogen, Carlsbad, CA) and TMB as described above ...
-
bioRxiv - Immunology 2020Quote: Whole genome transcript analysis was performed on 200 ng of total RNA using the Affymetrix Human Gene ST version 2.0 Array (Affymetrix). Genome-wide DNA methylation was profiled using the Illumina EPIC platform (Illumina ...
-
bioRxiv - Physiology 2020Quote: Cryo-preserved primary human hepatocytes from single donors (PHH; BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; Thermo Fisher Scientific) and the human HCC cell line HuH7 (ATCC ...
-
bioRxiv - Immunology 2019Quote: ... The plate was incubated with detection antibody (mouse anti-human IgG1 Fc conjugated to horseradish peroxidase clone HP6069; ThermoFisher) for 2 hours at room temperature ...
-
bioRxiv - Immunology 2019Quote: A cDNA encoding wild-type (WT) human TRIM22 (a gift form Professor Juergen Hass, University of Edinburgh) was inserted into pcDNA.3.1 (Invitrogen) in frame with a 5’ Myc-tag oligo to generate a pcDNA.Myc.TRIM22 ...
-
bioRxiv - Cell Biology 2020Quote: ... For RHBDL4 and OST4 depletion 50 pmol (RHBDL4) or 25 pmol (OST4) ON-TARGETplus SMARTpool human siRNA (Dharmacon) and 2.5 μl RNAimax (Invitrogen) transfection reagent per well of a 12-well plate were used ...
-
bioRxiv - Cell Biology 2019Quote: ... Human telomerase reverse transcriptase immortalized retinal pigment epithelial cells (TERT-RPE1, ATCC CRL-4000) were cultured in DMEM:F12 (Gibco) supplemented with 10% FBS and 1% penicillin and streptomycin ...
-
bioRxiv - Cancer Biology 2020Quote: Copy number analysis was derived from profiling of total DNA on the Affymetrix genome-wide human SNP Array 6.0 chip using instrumentation and protocols from Affymetrix. The raw CEL files were returned and the QC steps were performed using Affymetrix Genotyping Console ...
-
bioRxiv - Genomics 2019Quote: Human islets were washed once in cold PBS and dissociated into single cells by enzymatic digestion with Accumax (Invitrogen), followed by digestion using freshly prepared Dispase (Fisher Scientific) ...