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Citations for Thermo Fisher :
6501 - 6550 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: MS raw data were searched against the UniProtKB human sequence data base (92,954 entries) and TMT 10plex quantitation performed using Proteome Discoverer software (v 2.3; Thermo Scientific). Search parameters were set to include carbamidomethyl (C ...
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bioRxiv - Genetics 2024Quote: ... 3% human serum (heat-inactivated from Sigma-Aldrich, St. Louis, MO, USA or Thermo Fisher Scientific, Waltham, MA, USA), 2% human plasma (isolated from umbilical cord blood provided by Stanford Binns Cord Blood Program) ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: The human RPE cell line ARPE-19 was cultured in DMEM/F-12 (Cat #C11330500BT, Gibco, Waltham, MA, USA) media with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... falciparum erythrocytic stages were cultured in human erythrocytes (National Blood Transfusion Service, UK) and RPMI 1640 medium (Life Technologies) supplemented with 0.5% Albumax type II (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... human mammary fibroblast (MF) and cancer associated fibroblast (CAF) were maintained in DMEM/F-12 (Thermo Fisher Scientific, #21331046) supplemented with 2 mM Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... falciparum (3D7, Dd2, and HB3) parasites were cultured in 3-5% human O+ RBCs in RPMI-1640 (Gibco, 110875093) complete medium containing 0.5% Albumax-II (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by washing and addition of 100 µL/well of goat anti-human IgG-HRP conjugate (Invitrogen, 62-8420) at 1:500 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... followed by staining with Alexa Fluor 647-conjugated Goat anti-Human antibody (Thermo Fisher Scientific, A-21445, 1:500).
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bioRxiv - Biophysics 2024Quote: The human melanoma cell line MV3 [32] was cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, 11504496) containing L-Glutamine and Sodium Pyruvate ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was analyzed for gene expression changes using a microarray analysis (Clariom S Human HT, Thermo Fisher Scientific). Each replicate (100ng of total RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Neuroscience 2023Quote: Human dermal fibroblasts were obtained from skin biopsies and cultivated in Dulbecco’s modified eagle medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2024Quote: The full-length wild-type human NTCP gene (UniProt ID: Q14973) was generated synthetically by GeneArt (Thermo Fisher Scientific) after codon optimization for expression in HEK293 cells ...
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bioRxiv - Genomics 2023Quote: ... cDNAs from the human KZFPs were codon-optimized and synthesized using the GeneArt service from ThermoFisher (former Life Technologies). Sequences were cloned into the doxycycline inducible expression vector pTRE-3HA which yields a C-terminally tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Human dispersed islets were seeded at a density of 5×104 cells/cm2 on ultra-low attachment plates (ThermoFIsher) and allowed to form spontaneous pseudoislets ...
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bioRxiv - Microbiology 2023Quote: Human telomerase immortalized fibroblasts (MRC5-hT) and HEK 293T cells were cultured in Dulbecco’s Modified Eagle Media (DMEM; Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Bioengineering 2023Quote: ... The human dermal fibroblasts were cultured in a reconstituted Complete medium composed of DMEM medium (Dulbecco’s Modified Eagle Medium Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated at 37 °C for 1 hour followed by adding anti-human Alexa Fluor-647 (Invitrogen) secondary Abs for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... C2C12 myotubes and biopsy- derived human primary myotubes were transfected with plasmids using lipofectamine 3000 (Thermofisher, Waltham, MA, USA) following the supplier’s instructions.
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney 293T (HEK293T) cells were grown in complete DMEM (Dulbecco’s Modified Eagle’s Medium (DMEM, Invitrogen, Carlsbad, CA) containing 5% fetal bovine serum (Corning) ...
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bioRxiv - Neuroscience 2023Quote: ... and Gentamycin 40µg/mL and Human embryonic kidney (HEK) 293T cells were cultured in DMEM (1g/L glucose) (GIBCO) supplemented with 10% Fetal Calf Serum (FCS) ...
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bioRxiv - Immunology 2022Quote: ... at 10ng/ml final concentration along with dynabeads at a ratio of 25µl Human anti CD3/28 labelled Dynabeads/10 million cells (#11132D, Gibco).
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bioRxiv - Molecular Biology 2023Quote: ... Human β-arrestin 2 (residues 1-393, with I386A, V387A, and F388A mutations) was cloned into pBiT2.1 vector (Invitrogen) with the SmBiT at its N-terminus ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum and activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) at a 1:1 bead:cell ratio according to the manufacturer’s instructions ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... T cells were plated at 5×104 per well and stimulated with human CD3/CD28 T-Activator DynaBeads(Gibco) at 1:8 beads:T cell ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... was used to prepare samples and to facilitate hybridization to the human Clariom™ D arrays (Thermo Fisher Scientific). Transcriptome Analysis Console (TAC ...
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bioRxiv - Molecular Biology 2023Quote: ... ATCC CCL-81) and human embryonic kidney cells (HEK293T; ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2023Quote: ... Thawed Primary human hepatocytes were recovered in Cryopreserved Hepatocyte Recovery Medium (Cat. CM700, Thermo Fisher Scientific, Waltham, MA, USA), and re-suspended in ice-cold HBSS prior to surgery ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody used was R-Phycoerythrin labelled Goat anti-Human IgG Fc (eBioscienceTM; 12-4998-82, Invitrogen).
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bioRxiv - Immunology 2023Quote: CHO cells that were stably transfected with human CTLA-4 have been reported.23 CHO cells were grown in DMEM (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Cell Biology 2023Quote: ... Human microglial cells were cultured in cell culture flasks coated with 50 μg/ml rat tail collagen I (Gibco) in high glucose DMEM (4.5 g/l ...
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bioRxiv - Neuroscience 2023Quote: ... iPSC lines were generated from human peripheral blood CD34+ hematopoietic stem cells (HSC) with a CytoTune-iPS 2.0 Sendai Reprogramming Kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The human embryonic kidney 293T/17cells (ATCC, CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (Gibco Life Technologies) containing 10%FCS ...
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bioRxiv - Microbiology 2023Quote: ... The human Rhabdomyosarcoma muscle cell line (RD, ATCC CCL-136) was maintained in Dulbecco’s Modified Eagles Medium (DMEM, Gibco) containing 10% FBS and 1% P/S ...
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bioRxiv - Microbiology 2023Quote: ... the human microbiome database was used as described by Li et al.30 and analyzed using Proteome Discoverer 2.5 (Thermofisher). For subsequent data analysis ...
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bioRxiv - Microbiology 2023Quote: Human pharyngeal carcinoma Detroit 562 epithelial cells (ATCC_CCL-138) were grown in 10% FCS in alpha MEM media (Gibco). For each set of experiments ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The human erythroleukemia cell line TF-1 (ATCC, USA) were cultured in RPMI-1640 media (Thermo Fisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... Two different lots (lot 1355434 and 1767629) of human dermal neonatal foreskin fibroblasts (hDFn) were purchased from Thermo Fisher Scientific (cat ...
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bioRxiv - Bioengineering 2022Quote: Human osteosarcoma U2OS cell lines (HTB-96, ATCC, USA) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, GIBCO, USA) containing 10% heat- inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: Enriched neutrophils were resuspended at 1×106 cells/mL in RPMI with 20 ng/mL GM-CSF (R&D or STEMCELL) (for murine neutrophils) or RPMI with 2% FBS (for human neutrophils) with 1μM Sytox Green (Invitrogen) and plated in 96-well plates at 200,000 neutrophils per well ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were grown to log phase in the presence of 1:200 pooled normal human serum (Thermo Fisher Scientific) to opsonize bacteria ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA encoding full-length human CLASP2α was purchased from Dharmacon (Accession: BC140778.1) and subcloned into a pFastBacHT vector (Invitrogen) containing an N-terminal 6xHis-tag ...
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bioRxiv - Cell Biology 2022Quote: ... Endometrial ADGR gene expression in these 21 samples was determined using the high-density human genome (HG) U133 Plus 2.0 Arrays (Affymetrix), containing 42,203 genes and 12,397 expressed sequence tags (ESTs) ...
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bioRxiv - Cancer Biology 2023Quote: Human embryonic kidney 293T (HEK293T) cells were purchased from ATCC and cultured in DMEM media (Thermo Fisher, 11965-092) with 10% fetal bovine serum (Hyclone) ...
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bioRxiv - Cancer Biology 2023Quote: ... The human GCB-DLBCL cell line SU-DHL4 (RRID:CVCL_0539) was obtained from DSMZ and grown in RPMI 1640 (ThermoFisher; 11875093) with 10% FBS ...
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bioRxiv - Genomics 2023Quote: ... in condΔEXP168 were maintained in 3% hematocrit in human O+ blood in RPMI 1640 supplemented with 5% Albumax-II and 50µg/ml hypoxanthine (Gibco), 25mM HEPES (Sigma ...