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Citations for Thermo Fisher :
6651 - 6700 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was transfected into human osteosarcoma U-2 (U2OS) cells by electroporation using the Neon Transfection System (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... were used to retro-transcribe 1 μg of total RNA from Human Liver Total RNA (ThermoFisher Scientific, Waltham, MA, USA). The reverse transcriptase reaction for the viral pellet was carried out with random primers using a commercial kit (EasyScript cDNA Synthesis kit ...
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bioRxiv - Neuroscience 2019Quote: ... or 1 µg of pCMV-SPORT6.FBXL3 and 40 nM ON-TARGETplus human CUL1 (8454) siRNA SMARTpool (Dharmacon, L-004086) using the Neon transfection system (Invitrogen) following the manufacturer instructions and 1400 V ...
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bioRxiv - Biophysics 2019Quote: Jurkat T cells (Human T cell leukemia cell line– ATCC TIB-152) were grown in RPMI-1640 with Glutamax (GIBCO), supplemented with 10 % foetal calf serum and ...
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bioRxiv - Biochemistry 2020Quote: The heavy and light chain sequences of mAb45D825 were separately cloned into pcDNA3.4 and the resulting vectors were transfected into Expi293F Human Embryonic Kidney cells (Life Technologies) using a 1:2 mass ratio of light and heavy chain DNA with the Expifectamine transfection kit (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... falciparum cell lines were cultured in 5% human O+ red blood cells (Australian Red Cross blood service) in RPMI-GlutaMAX-HEPES (Invitrogen) supplemented with 5% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... an in-house generated model of human hepatic Huh-7 cells stably overexpressing mRFP-Rab5 were cultured in DMEM/GlutaMax/High glucose (Gibco) supplemented with 10 % FBS ...
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bioRxiv - Bioengineering 2019Quote: ... envelope and one of these transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s lentiviral production protocol (Thermo Fisher Scientific Inc. ...
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bioRxiv - Developmental Biology 2019Quote: Human astrocytoma-derived 1718 cells [CCF-STTG1 (ATCC® CRL-1718TM)] were cultured in RPMI 1640 medium (ATCC modification, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2019Quote: U-2 OS cells (human bone osteosarcoma epithelial cells, ATCC Number: HTB-96 [17]) were grown in D-MEM Glutamax (Gibco) supplemented with final concentrations of 10% fetal calf serum (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: ... Primary neonatal Human Umbilical Vein Endothelial Cells (HUVEC) were sourced from three suppliers to provide genetic diversity (Lonza, Gibco, PromoCell). HUVECs were expanded in endothelial cell growth medium (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... and CD19+ B-cells were isolated by negative selection using the Dynabeads® Untouched™ Human B Cells kit (Invitrogen). Mantle cell lines (Z-138 ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2019Quote: ... Immortalized human umbilical vein endothelial cells (iHUVEC, gift from Dr. W. Muller, Northwestern University [NU]) were cultured in human endothelial serum-free medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting supernatant was decanted and the concentration of secreted IL6 was measured by the human IL6 ELISA kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice with PBS and stained with 2 μg/ml goat anti-human (Alexa Fluor 647; Invitrogen) secondary Abs for 15 min in PBS ...
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bioRxiv - Genetics 2019Quote: ... Extracted DNA samples were quantified following the Quantifiler Duo Human DNA Quantification Kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) as recommended by the manufacturer in 7500 Real-time PCR System (Thermo Fisher Scientific Company ...
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bioRxiv - Cell Biology 2021Quote: ... of the MCF7 human ductal breast carcinoma cell line[64](hereafter referred to as MCF7) was cultured in RPMI medium (Gibco) supplemented with 6% FCS and in a humidified incubator set to 37°C and 5% CO2 ...
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bioRxiv - Genomics 2021Quote: Human osteosarcoma (U2OS, ATCC HTB-96) cell lines were maintained in McCoy’s 5A Modified Medium (16600082, Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were activated for two days with Human T-Activator CD3/CD28 Dynabeads (ThermoFisher Scientific, Waltham, MA). After two days ...
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bioRxiv - Microbiology 2021Quote: ... NL63-CoV and the N-terminal peptidase domain of wild-type and human ACE2 SNVs were expressed using the Bac-to-Bac baculovirus system (Invitrogen). Specifically ...
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bioRxiv - Bioengineering 2021Quote: All experiments used a previously described research cell bank of T-REx-293 human embryonic kidney (HEK) cells stably expressing the tetracycline repressor protein (ThermoFisher) (4) ...
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bioRxiv - Bioengineering 2020Quote: ... R&D systems sheep anti-human CD31/PECAM-1 antibody (AF806) and Hoechst 33324 (H3570) were obtained from Thermo Fisher Scientific (Whaltam ...
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bioRxiv - Bioengineering 2021Quote: ... 105 PBMCs derived from a healthy human donor were stimulated with T cell activating beads (anti-CD3/CD28 Dynabeads, ThermoFisher) at a 1:1 PBMC:bead ratio in each well containing MSCs ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human astrocytes were grown on the bottom of transwell inserts (24-well, 0.1 um pore size, PET, Fisher Scientific) which were coated with poly-L-lysine (20 ug/mL) ...
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bioRxiv - Biochemistry 2021Quote: Codon optimized human SERT was assembled from synthetic DNA fragments (IDT) and inserted into the NheI-XhoI sites of pCEP4 (Invitrogen). The use of a synthetic gene ensures there are no sequence features ...
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bioRxiv - Neuroscience 2021Quote: ... The tandem mass spectra were searched using SEQUEST 80 search algorithms against a Uniprot database (human 2019_07 release for 293T CoIP and mouse 2019_08 release supplemented with human TDP-43 protein sequence for mouse CoIP) through the Proteome Discoverer platform (version 2.5, Thermo Scientific). The search parameters included a maximum of two missed cleavages ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were generated by RUCDR Infinite Biologics® from human primary lymphocytes using Sendai viral vectors (CytoTune™, ThermoFisher Scientific), as previously described (Moore JC et al ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was produced in 293T cells and human melanoma cell lines were infected and selected with 1 μg/mL Puromycin (Gibco) for 3 days ...
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bioRxiv - Cancer Biology 2021Quote: Isolated human PBMCs were seeded and grown in 6 well culture dish with hybridoma-(SFM) serum free medium (Cat #12045076, Gibco). PBMCs were treated with patient EVs under serum free conditions for 24 hrs ...
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bioRxiv - Cell Biology 2021Quote: Human foetuses were obtained following elective termination of pregnancy with full consent and stored overnight in Hibernate-A Medium (Gibco) at 4° C ...
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bioRxiv - Cell Biology 2021Quote: MS/MS spectra were searched against a Swiss-Prot human database containing 20,341 reviewed protein entries using Mascot algorithm (Matrix Science) via Proteome Discoverer 2.2 (PD, Thermo Fisher Scientific) and were processed as previously described (10) ...
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bioRxiv - Cell Biology 2021Quote: ... A probe mix containing 60 ng of each library probe and fluorescently labeled readout probe and 40 μg human COT1 DNA (Invitrogen) in 1.1 ml of hybridization buffer (20% dextran sulfate ...
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bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with the secondary antibody diluted in a blocking buffer solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse 594 (1:400 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1×106 cells were stained with NCAM-1 antibody (CD56 Anti human Alexafluor 700, 1:200 dilution, Fisher Scientific, BDB557919) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... NOK1 cells were grown in keratinocyte-SFM medium supplemented with human EGF and bovine pituitary extract (BPE) (Thermo Fisher Scientific) and induced to differentiate by transfer to Dulbcecco modified Eagle medium (DMEM ...
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bioRxiv - Cancer Biology 2020Quote: ... HDL was labeled with Alexa568 by incubating 4.5 mg Human HDL in 90 μl 1 M sodium bicarbonate with Alexa Fluor 568 succinimidyl ester (A20003, Thermo Fisher) for 1 hr at room temperature (8:1 dye:estimated apoA1 mole ratio) ...
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bioRxiv - Cancer Biology 2019Quote: ... human GRPR shRNA in Lentiviral Vector (Genomics Online; Limerick, PA, # ABIN3479889) and mouse Grpr siRNAs (Life Technologies, #157912 and #157914) were used for all knockdown experiments ...
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bioRxiv - Cell Biology 2021Quote: Human non-transformed retinal pigment epithelial cells transduced with the human telomerase gene (hTERT-RPE1; ATCC CRL-4000) were grown in DMEM/F12 (Gibco) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Bioengineering 2020Quote: ... 2-3 ⨯ 105 cells were collected for each condition and samples were stained with the following antibodies: APC-conjugated anti-human CD4 (Invitrogen), FITC-conjugated anti-human CD4 (BD Bioscience) ...
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bioRxiv - Biochemistry 2021Quote: The knock-out cell line (GTPBP5KO) was generated in the Flp-In T-Rex human embryonic kidney 293 (HEK293T) cell line (Invitrogen) using CRISPR/Cas9 technology targeted on exon 1 of MTG2 gene ...
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bioRxiv - Cell Biology 2021Quote: Human islet and hESC-derived differentiated organoids (approximately 20 organoids or human islets per well) were respectively plated into a 96-well black plate (ThermoFisher) coated with 1% hESC-qualified Matrigel ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Microbiology 2020Quote: ... human serum or oseltamivir were serially two-fold diluted in Dulbecco’s phosphate buffered saline with calcium and magnesium (ThermoFisher Scientific) containing 1% BSA (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... ATCC) and HeLa (a human cervical cancer cell line, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, USA) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Immunology 2021Quote: ... Primary antibodies were diluted 1:100 in PBS-T as follows: single labeling: CD14 (mouse anti-human, 14-149-82, Invitrogen), CD56 (mouse anti-human ...
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bioRxiv - Microbiology 2021Quote: ... the bound antibody was detected with goat anti-human Ig (γ chain specific)-HRP conjugated secondary antibody (Thermo Fisher Scientific) diluted 1:10,000 in blocking buffer ...