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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... GSCs were cultured as spheres on non-tissue culture-treated 10cm plates or as adherent cultures on laminin on tissue culture-treated 10 cm plates (ThermoFisher Scientific). GSCs were grown in DMEM/F12 media ...
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bioRxiv - Synthetic Biology 2019Quote: Each plate containing the isolated clones was used to inoculate 200-µl aliquots placed on two replica 96-well plates for fluorescence measurement (Costar black plates with clear bottom; Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μl of each supernatant was transferred into a 96 well plate and the absorbance at 562 nm was measured using a Varioscan plate reader (Thermo scientific). In order to evaluate the degree of nanoparticle interference ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μl of samples were transferred to a microwell plate (Nunc™ 3 84-Well Optical Bottom Plates # 242764, Thermo Scientific) and the time course of fluorescence-intensity (see Fig ...
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bioRxiv - Cancer Biology 2020Quote: ... by using a Fluorostar Omega plate fluorescence plate reader (540 nm excitation, 590 nm emission) after incubation with 10% Presto Blue (Life Technologies) for 1h at 37C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HK-2 was seeded in 6 well plates (1 × 106 cells/well) or 96 well plates (1 × 105 cells/well) and cultured in 90% RPMI1640 medium (Gibco, USA) supplemented with 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... pombe cells were pre-cultured to exponential phase in 180 μl of YES in 96-well culture plates at 32°C in the VarioSkan Flash plate reader (Thermo Scientific). 5 μl of the preculture were used to inoculate 175 μl of fresh YES in 96-well culture plates and incubated for ∼48 hours in the VarioSkan Flash plate reader at 32°C with background shaking (1080 spm ...
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bioRxiv - Systems Biology 2022Quote: ... filtered by positive pressure and the flowthrough was collected in a glass coated microtiter plate (WebSeal Plate+ 96-Well Glass-Coated, 300 - Fisher Scientific); 3 ...
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bioRxiv - Neuroscience 2023Quote: ... for ten minutes in a 96-well plate (protected from light) and the fluorescence was read at 520 nm using a Varioskan Flash plate reader (ThermoFisher, UK).
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Plant Biology 2024Quote: ... 3 μl of the dilution series and cocktail mix were dispensed into duplicate wells on a 384-well ABI optical plate and the plate was loaded on the ABI 7900HT Fast Real-Time PCR System (Applied Biosystems). The FAM-Non Fluorescent Quencher detector and Rox passive reference were selected ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and then transfected with 100 ng/well (96-well plates) or 500 ng/well (24-well plates) indicated plasmids using Liopfectamine 3000 (Invitrogen, USA) for 24 h or as specified ...
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bioRxiv - Microbiology 2023Quote: ... and 0.2 μl reverse primers (5 μM) in 96-well plates (MicroAmp Fast 96-well Reaction Plate, Applied Biosystems for Life Technologies). The reactions were performed in triplicates on a QuantStudio 5 Real-Time PCR System (Thermo Fischer Scientific ...
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bioRxiv - Physiology 2023Quote: ... From the tubes 300μl of all samples were transferred to ELISA plate in triplicates and the absorbance was read at 520nm using ELISA plate reader (ThermoFisher™). Optical Density (OD ...
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bioRxiv - Immunology 2024Quote: High-throughput screening of antibodies was done using Expi293 cells grown in 3 mL plates in 24 deep-well plates (Thermo Fisher). Cultures were grown for four days ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM) and diluted in DMSO transferred to wells of PCR Plates (Micro Amp Fast 96-well Reaction Plates, Applied Biosystems) to ensure matching final DMSO concentrations.
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bioRxiv - Cancer Biology 2023Quote: ... siRNAs were transfected at 10ng/well in 384 well plates and 40ng/well in 96 well plates using Lipofectamine RNAiMAX (Invitrogen, #13778075). Imaging and drug incubation experiments were conducted in 384 well plates ...
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bioRxiv - Biochemistry 2022Quote: ... Bacterial growth was monitored by measuring OD600 and for growth experiments performed in airtight sealed microtiter plates (sealing tape for 96-well plates, Thermo Scientific), a Power Wave XS microplate reader (BioTek Agilent ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μL aliquots of infected cells were deposited in a white 96-well plate (F96 MicroWell™ Plates, non-treated form Nunc) and the luminescent signal was measured with the help of the plate reader.
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bioRxiv - Biophysics 2023Quote: ... 8 µl of ATP-ATPase reaction mix were transferred from row A on master mix plate to respective row (B to H) on reaction stop plate using a MatrixTM Multichannel Pipette (Thermo Fisher). Additionally ...
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bioRxiv - Immunology 2023Quote: ... The diluted samples were transferred from bulk dilution plates into individual 384-well black tissue culture plates at 30 µL per well (Thermo Scientific Nunc 384-well polystyrene plates ...
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bioRxiv - Microbiology 2023Quote: ... pre-cultures were prepared by inoculating single colonies from a LB agar plate in 200 μl of liquid LB in a 96-well plate (Nunc, Thermo Scientific). After 22 h of growth at 37°C with 250 rpm shaking ...
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bioRxiv - Microbiology 2023Quote: ... gondii growth were determined in HFF cell cultures using 96-well black plates (Optical-Bottom Plates with Polymer Base, ThermoFisher Scientific). Sulfadiazine (positive control ...
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bioRxiv - Immunology 2023Quote: ... The diluted samples were transferred from bulk dilution plates into individual 384-well black tissue culture plates at 30 µL per well (Thermo Scientific Nunc 384-well polystyrene plates ...
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bioRxiv - Microbiology 2024Quote: ... and GFP fluorescence (excitation 485 nm, emission 520 nm) were measured in microtiter plates using a Varioskan Lux plate reader (Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... monolayer permeability was assessed by transferring the transwell plates in a new receiver plate containing 200 µl Hank’s balanced salt sodium (HBSS) (Gibco 14025-092). To determine permeability of the monolayers (1 mg/ml ...
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bioRxiv - Neuroscience 2019Quote: Plated DRG cultures were loaded with fura-2 by 1-hour incubation with a mixture of 2 μM fura-2 AM (Thermo Fisher Scientific) and 0.01% pluronic acid (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-d-glucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Trypsin-EDTA solution was purchased from GIBCO BRL (Grand Island ...
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bioRxiv - Microbiology 2023Quote: The proteolytic activity of activated globupain and its substitution variants was assessed using the casein gelzan™ CM plate assay and the EnzChek™ Protease Assay Kit (Thermo Fisher Scientific, MA, USA), respectively ...
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bioRxiv - Cell Biology 2020Quote: S phase status was monitored by measuring 5-ethynyl-2’-deoxyuridine (EdU) incorporation with the Click-iT™ Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (ThermoFisher, C10632) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total cellular mRNA was isolated from cells grown in 2-D and 3-D culture conditions using the GeneJET RNA purification kit (Thermo Fisher Scientific). For analysis of germ layer markers ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products detected on 6% polyacrylamide or 2% agarose gels were purified with a Charge Switch PCR Clean-Up kit (Invitrogen, Carlsbad, CA) and then sent to BMR genomics (Padova ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg of RNA were reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit with RNAse Inhibitor (Applied Biosystems, Vilnius, Lithuania). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Lungs were homogenised with a FastPrep-24 5G instrument using TallPrep Lysing Matrix M tubes (MP, Cat# 116949025) in 2 ml Trizol and RNA was isolated using the RiboPure Kit (Invitrogen, Cat# AM1924), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were pulse labeled for 2 hours with 10 µM EdU and stained using the Click-iT EdU Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... To create the lag-2 full-length construct we used the Multisite-Gateway Three-Fragment Vector construction kit (Thermo Fisher Scientific, 2012). 60ng /µl of this 17.623bp long lag-2 full-length construct was injected together with the co-injection markers (pCFJ601 50ng/µl ...
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bioRxiv - Cell Biology 2019Quote: ... staining was performed in three-dimensional cysts with 2 mM calcein AM and 4 mM ethidium homodimer I (Live/Dead® Viability/Cytotoxicity kit; Life Technologies) for 40 min at 37°C in the dark ...
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bioRxiv - Developmental Biology 2020Quote: ... gRNAs were visualized in a 2% agarose stained by ethidium bromide to check for RNA integrity and quantify using Qubit™ RNA BR Assay Kit (ThermoFisher, Q10210). gRNAs targeting tbxta and tyr CDS in zebrafish and rx3 in medaka were in vitro transcribed from PCR containing a pool of different gRNA primers at equal concentration ...
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bioRxiv - Biochemistry 2020Quote: ... Each sample was labeled for 2 h at 22°C with a different TMT-tag obtained from a TMT10plex kit (Thermo Fisher Scientific), and then quenched (0.27% hydroxylamine ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 2 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA). Quantitative PCR reactions and measurements were performed with a CFX384 Real-Time PCR detection system or an iQ5 Multi-color real-time PCR detection system (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was treated with 2 U of DNase per 10 μg of total RNA using the TURBO DNA-free™ Kit (Thermo Fisher Scientific, former Ambion). Integrity of DNA-free RNA was verified in Bleach Gel (Aranda et al. ...
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bioRxiv - Systems Biology 2021Quote: Cells were harvested at a concentration of 1-3 × 106 cells/mL and resuspended at 2 × 107 cells/mL in 800 μL of Reagent A from the Mitochondrial Isolation Kit for Cultured Cells (Thermo Fisher #89874) on ice ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 2 µg of total RNA using oligo(dT) primers and Superscript II First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific). Quantitative RT-PCR was performed with FastStart Universal SYBR Green Master Mix and a LightCycler 96 Real-Time PCR System (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
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bioRxiv - Developmental Biology 2021Quote: ... Purified RNA (2 µg) was used to perform cDNA synthesis using the ABI High Capacity cDNA Reverse Transcription Kit (Thermo Fisher 4368814) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... (all the primer sequences are listed in Table 1 and the PCR conditions are listed in Table 2) respectively.The PCR product were purified using Quick gel extraction kit (Invitrogen: Cat no. # K210012). The purified double-stranded 5′UTR fragment and 3′UTR fragment (containing overhangs for insert and vector fragment ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Total RNA (2 μg) was reverse transcribed using a Superscript II® Reverse Transcriptase Kit and random primers (Invitrogen Canada, Burlington, ON). Reactions were incubated at 42°C for 50 min and at 90°C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequencing reactions were performed with the same primers as the ones used for the primer extension reactions and with PCR fragments (amplified from phi14:2 genomic DNA) using the USB Thermo Sequenase Cycle Sequencing Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... GFAP and MAP-2 mRNA expression was performed with the DyNAmo™ Flash SYBR® Green qPCR Kit (Thermo Scientific, Runcorn, UK) using a 7900 HT Fast Real-Time PCR System (Applied Biosystems ...