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Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The extracted DNA was quantified in duplicate using the fluorescent-based Qubit® quantitation assay (Thermo Fisher Scientific, cat #Q32850). DNA stocks were diluted to 10-30 ng/ul and then combined at equimolar concentrations into pools of 11-25 individuals per population ...
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bioRxiv - Cell Biology 2020Quote: ... Images for calibration of position and scale/magnification were captured using multi-wavelength fluorescent beads (TetraSpeck 0.1 μm Microspheres, Invitrogen T7279), then the red and green images aligned using the same approach we previously used for multi-focal plane microscopy (Walker and Wheeler ...
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bioRxiv - Bioengineering 2021Quote: ... and exposed to 25 µM DRAQ5 Fluorescent Probe for 5 min at room temperature under agitation (#62254, Thermo Scientific™).
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bioRxiv - Neuroscience 2021Quote: ... incubated in the same solution as the primary antibodies with the corresponding secondary donkey Alexa fluorescent antibodies (1:1000, ThermoFisher) for 2hr ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescent intensity for each well was measured using the 485/520 excitation/emission channel of a Varioskan Lux (ThermoFisher #VL0000D0) plate reader ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were detected using the fluorescent secondary antibodies goat anti-rabbit Alexa 488 (1:400, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse Alexa 546 (1:400 ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... and cell numbers were quantified using the Celigo Image Cytometer (Nexcelom Bioscience) and a fluorescent Countess II FL Automated Cell Counter (Invitrogen).
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bioRxiv - Cancer Biology 2019Quote: ... GFP expression was evaluated under a fluorescent microscope and cells were selected using 500 μg/mL of G-418 antibiotic for 4 days (Gibco). Cells used for analysis were >70% GFP-positive.
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bioRxiv - Neuroscience 2019Quote: ... Sections were then stained with 1:200 NeuroTrace 640/660 Deep-Red Fluorescent Nissl Stain (Thermo Fisher, N21483, RRID: AB_2572212) for 10 min and mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... For imaging experiments we used: (i) a sample of 100 nm fluorescent beads (yellow-green FluoSpheres Q7 Carboxylate-Modified Microspheres, F8803; Invitrogen). We treated the glass coverslips with poly-L-lysine (P8920 ...
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bioRxiv - Cell Biology 2021Quote: ... The optimal digitonin concentration varied by cell density and passage number and was optimized prior to each assay such that the majority of plasma membranes were permeabilized while maintaining nuclear exclusion of a 70 kD fluorescent dextran (ThermoFisher). The permeabilized cells were washed twice in ice-cold TRB prior to initiating the export assay.
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bioRxiv - Cell Biology 2021Quote: ... sections were washed at least 3-5 times with TBSTX buffer and incubated with the appropriate Alexa fluorescent secondary antibodies (Invitrogen). Sections were washed 3-5 times with TBSTX buffer and then mounted on Superfrost slides (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... with small modifications after the addition of the mitochondrial membrane potential MitoTracker® Deep Red FM fluorescent dye (Invitrogen no.M22426) at 0.6 μM to identify live parasites (Supplementary Fig ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies conjugated with Alexa Fluor fluorescent proteins (488, 568, 647) were used in dilution of 1:200 (Molecular Probes no ...
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bioRxiv - Neuroscience 2020Quote: ... The target molecules were visualized with horseradish peroxidase using the DAKO ARK kit according to the manufacturer’s instruction or with fluorescent-conjugated secondary antibodies: goat anti-mouse IgG (H+L) with Alexa Fluor 594 (Invitrogen, A32742); goat anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... A burr hole was made to inject the fluorescent muscimol Bodipy(R)-TMR(X) (500 μM in cortex buffer with 5% DMSO, Invitrogen) in the medial part of the posterior thalamic nucleus (POm) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 100 ng of yellow fluorescent protein (YFP) cDNAs in each well using 1:3 DNA: Lipofectamine 2000 (11668019, Invitrogen) ratio ...
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bioRxiv - Microbiology 2021Quote: ... 2017) and using the point-spread function (PSF) of the microscope (based on 100 nm fluorescent beads (TetraSpeck, Thermo Fisher)) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202 ...
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bioRxiv - Cell Biology 2021Quote: ... GFP or GFP-aPKCi cells were induced 24h before seeding. Red fluorescent (exc. 580/em. 605nm) 2μm diameter latex beads (ThermoFisher F88265) were endocytosed overnight ...
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bioRxiv - Biophysics 2020Quote: ... Nile Red (AC415711000), DiI-C18(5) (D3911), carboxylate-modified microspheres (0.1 μm, 350/440 blue fluorescent, F8797) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... mice at P17 were briefly warmed under heat lamp to dilate tail veins before injection of microspheres (1% solution of 25 nm fluorescent microspheres; 50 μl per mouse into the tail vein; ThermoFisher). Microspheres circulated for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The measurements were performed by a fluorescent reader at 360 nm excitation/460 nm emission (Varioscan, Thermo Fisher Scientific, US). While DMSO was used as solvent control ...
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bioRxiv - Biophysics 2022Quote: Fluorescent labeling was performed by targeting histone amine groups of pre-assembled nucleosomes using Alexa Fluor 647 succinimidyl ester (ThermoFisher), following the previously reported strategy20 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then permeabilized with 0.2% Triton X-100 and treated with 300nM of DAPI fluorescent dye solution in PBS (Invitrogen, D3571). DAPI-stained cells were counted on a Cytation5 (BioTek ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for an hour at room temperature in secondary solution containing the fluorescent markers goat anti-rabbit 488 (A11034, 1:1000, Invitrogen). After one additional wash ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for an hour at room temperature in secondary solution containing the fluorescent markers goat anti-rabbit 488 (A11034, 1:1000, Invitrogen). After one additional wash ...
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bioRxiv - Neuroscience 2022Quote: ... Neuronal somata were visualized with a stain against the Nissl substance using the NeuroTrace 435/455 fluorescent dye (Invitrogen #N21479). Biocytin was visualized using streptavidin conjugated to Alexa Fluor 647 (Invitrogen #S21374) ...
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bioRxiv - Bioengineering 2022Quote: Human iPSC-derived Brain Microvascular Endothelial-like cells were treated with 25 μg/mL fluorescent transferrin conjugate (Thermo Fisher Scientific) and incubated at 37oC for 30 min ...
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bioRxiv - Biophysics 2022Quote: Molten M9DCAA 0.6% agar was mixed with 0.5 µm-diameter fluorescent microsphere (FluoSpheres carboxylate-modified, ex/em: 580 nm /605 nm; Thermofisher F8812) at a final microsphere number density of 3.1 x1011 particles/mL (∼1000-fold dilution of the microsphere solution) ...
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bioRxiv - Cell Biology 2022Quote: ... we immuno-stained the cells with fluorescent secondary antibodies to detect transfected cells and used the Prolong Gold antifade mounting medium (Thermofisher) instead of the Duolink in situ mounting medium provided with the kit.
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bioRxiv - Neuroscience 2021Quote: ... The cells were washed three times with PBS and incubated with the fluorescent conjugated secondary antibody (Molecular Probes, A-11001) in 1X Roti®-Block for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... SCs were then washed in PBS and incubated for 2 h at RT with secondary fluorescent antibodies (goat anti-rabbit-conjugated 649; donkey anti-guinea pig-conjugated Alexa Fluor 405 [1:1000, ThermoFisher]) diluted in 0.2% Triton X-100 blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... the brain sections were washed three times in PBS for 10 minutes and then incubated for 1.5 hours at room temperature with fluorescent secondary antibodies (AlexaFluor, Invitrogen Molecular Probes, Eugene ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with fluorescent-dye-conjugated secondary antibodies: goat secondary antibodies coupled to Alexa 488 (Molecular Probes, 1:200) or Alexa 555 (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... the astrocyte specific non-fixable red fluorescent dye sulforhodamine 101 (SR101, 80 μM in aCSF, Thermo Fisher, Waltham, MA, USA) was applied topically ...
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bioRxiv - Neuroscience 2020Quote: ... was applied on the back of the probe before insertion) was confirmed histologically on Nissl-stained 60 μm sections (NeuroTrace 500/5225 Green Fluorescent Nissl Stain, Cat#N21480, Invitrogen). Only experiments with appropriate position of the probe were used for analysis (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 minutes with PBS before incubation with streptavidin with fluorescent tag (Alexa Fluor 647 – Thermofisher) in blocking solution at 1:1000 dilution for 1.5hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed twice with PBS and incubated with fluorescent secondary antibodies (Anti-Rat Goat Alexa Fluor 488 [A-11070], Anti-Mouse Goat Alexa Fluor 647, Thermofisher) in PBSA-T for one hour at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... The entry clones were recombined into the Gateway binary vector pGWB552 tagged with G3 green fluorescent protein (G3GFP) using LR clonase (Invitrogen) (Nakagawa et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... U2OS cells stably expressing GFP tagged SESN2 were exposed to 10 μM FCCP treatment for 24h and before the fluorescent microscopy procedure were incubated with 100 nM MitoTracker Red (CMXRos, Invitrogen) in DMEM for 30 min at 37°C to stain mitochondria.
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bioRxiv - Microbiology 2021Quote: ... the green and red channel were aligned by image registration with fluorescent beads (TetraSpeck™ microspheres, 0.2 µm, Invitrogen™). Thus ...
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bioRxiv - Pathology 2021Quote: ... Samples were washed three times in PBS and incubated with the proper fluorescent secondary antibody (AlexaFluor 488/555/647, Invitrogen) diluted 1:400 in antibody diluent for two hours at room temperature ...
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bioRxiv - Biophysics 2020Quote: Wild-type and the three LXXAA mutants of TIF2NRID were amine-labelled with a fluorescent probe (Alexa 488 NHS Ester, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The experimentally measured PSFs were obtained separately for each color channel using fluorescent beads (200 nm FluoSpheres®, Thermo Fisher) coated on a coverslip66 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mL of the agar solution was repeatedly aspirated with 200 μL of a 1:500 dilution of 0.1 μm yellow-green fluorescent nanobeads (FluoSpheres®, Invitrogen) to evenly distribute beads in the agar solution ...
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bioRxiv - Neuroscience 2021Quote: ... or fluorescent secondary antibodies with species-specific Alexa Fluor-488 or -568 (Invitrogen A-11039 or A-11011, 1:250) to visualize proteins ...
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bioRxiv - Neuroscience 2021Quote: ... all wells were processed for 1 h with appropriate fluorescent secondary antibodies (Alexa Fluor 594 donkey anti-rabbit (1: 1,500, Life Technologies); Alexa Fluor 488 donkey anti-chicken (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were prepared on ice with a protein concentration of 0,5 μg/μl by addition of 4x Fluorescent Compatible Sample Buffer (#LC2570, Thermo Fisher) and 100 mM DTT ...
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bioRxiv - Developmental Biology 2022Quote: ... The point spread function for deconvolution was obtained with the use of fluorescent beads (diameter of 200 nm; F8811, ThermoFisher). After processing of images by Gaussian filtering ...