Labshake search
Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... First-strand cDNAs were synthesized from 2 µg of RNA using oligo(dT)18 primers and the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). The resulting cDNA was used for RT-PCR reactions performed with control primers for the housekeeping gene ACTIN and gene-specific primers for VIA1 ...
-
bioRxiv - Physiology 2024Quote: ... neonatal hearts from 1-2 days pups were dissected in cold Hanks’ Balanced Salt Solution (HBSS) and the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher, 88281) was used to obtain neonatal cardiomyocytes ...
-
bioRxiv - Neuroscience 2023Quote: ... Residual genomic DNA was removed from samples containing 2 μg of total RNA using the Turbo DNase-free kit (Invitrogen; Thermofisher Scientific) and about 1.8 μg of total RNA was reverse transcribed using a High Capacity RNA to cDNA kit (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: RNAs were transcribed in vitro from PCR products using primers listed in Table 2 and the MEGAShortScript T7 kit (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
-
bioRxiv - Biophysics 2023Quote: In vitro cytotoxicity of the complexes was evaluated using U-2 OS cells and an LDH assay kit.42 Cells were counted with a Countess II Automated Cell Counter (ThermoFisher Scientific, USA) and seeded in 96-well plates at a density of 10,000 cells per well in 100 μL of full DMEM ...
-
bioRxiv - Molecular Biology 2023Quote: ... MicroRNAs and U6 small nuclear RNA (supplementary table 2) were polyadenylated and converted into cDNA by using TaqManTM MicroRNA Reverse Transcription (RT) kit (Thermo Fisher Scientific). Amplification was visualized by StepOnePlus™ Real-Time PCR System (ThermoFisher Scientific).
-
bioRxiv - Synthetic Biology 2023Quote: ... A 2-mL aliquot of these cultures was used to isolate genomic DNA (gDNA) with the PureLink™ gDNA mini kit (ThermoFisher Scientific). Library construction ...
-
bioRxiv - Cell Biology 2023Quote: between 0.2·106-1·106 of VS derived cells were reprogrammed using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), a non-integrative cell reprogramming method holding the four Yamanaka factors (OCT4 ...
-
bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2024Quote: ... 2 µg total RNA was used for first-strand cDNA synthesis using a Maxima first-strand cDNA synthesis kit (Thermo Scientific, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... Phase separation in cells lysed in TRIzol was induced with 50% isopropanol and 0.5% 2-mercaptoethanol and RNA was extracted from the aqueous phase using the MagMAX mirVana Total RNA Isolation Kit (Thermo Fisher, A27828) on a KingFisher Flex Magnetic Particle Processor according to the manufacturer’s protocol with 1-2 million cells input ...
-
Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: Total RNA was extracted from meiotic cell culture of 1.5-2 OD600 (∼2.5 x107 cells) using TRIzol® reagent and PureLink® RNA mini kit (Ambion Life Technologies™). The cell pellet was re-suspended in TRIzol® and cell lysis was done using DEPC treated sterile 0.5 mm glass beads ...
-
bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR analysis was performed using Green PCR Kit (TransGen, Cat# 2-21) on a QuantStudio 7 Flex system (Thermo fisher, USA). The gene-specific primers were listed in Supplementary Table 2 ...
-
bioRxiv - Immunology 2024Quote: ... 2 μg total RNA was reverse transcribed to give cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Invitrogen, USA). Quantitative real-time polymerase chain reaction (qRT-PCR ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Samples were diluted to a final concentration of ~2 nM after they were quantified using the Qubit dsDNA HS Assay kit (Invitrogen, Catalog #Q33231). The libraries were then run on either an Illumina MiSeq using a standard 300-cycle kit or an Illumina NextSeq 500 or an Illumina NextSeq 2000 using a mid-output 300-cycle kit using standard protocol.
-
bioRxiv - Immunology 2024Quote: ... 2 μg total RNA was reverse transcribed to give cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Invitrogen, USA). Quantitative real-time polymerase chain reaction (qRT-PCR ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Libraries were grown in 10 mL LB for 2-3 h and plasmids extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). From each library ...
-
bioRxiv - Microbiology 2020Quote: ... and 20 μl of such mixtures were loaded on 96-well white plates (Microamp Optical 96-well Reaction Plate with Barcode, Applied Biosystems, #4306737), containing 5 μl of an appropriate 5x buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... OD600 and fluorescence were measured on a Biotek Synergy H1 MF plate reader using clear-bottom 96-well plates (Thermo Scientific Nunc, #265301). Prior to plate reader measurements ...
-
bioRxiv - Molecular Biology 2019Quote: ... The experiments were carried out using the CLARIOstar plate reader (BMG Labtech; settings: orbital reading 4 mm (for 96-well plates from Nalge Nunc International) or 2 mm (for 96-well plates from Thermo Scientific) ...
-
bioRxiv - Immunology 2019Quote: ... 1×106 (24-well plate) or 5×105 (48-well plate) CD8+ T-cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher, 11456D) in a 1:1 beat to T-cell ratio and cultured in 2 ml (24WP ...
-
bioRxiv - Microbiology 2019Quote: ... The solution was transferred into a fresh plate and absorbance measured at 590 nm with a plate reader (Multiskan Go, Thermo Scientific, USA). Results were exported with the software SkanItTM (Thermo Scientific ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Microtiter plate cultivations were performed in sterile 96-well plates (flat bottom Nunclon™ Delta Surface, Thermo Fisher Scientific, Waltham, MA, US) containing 200 μL LB per well ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were washed three times in DPBS and 100 μl of DPBS was added to each well and plates were sealed with adhesive plate tape (Thermo Fisher Scientific).
-
bioRxiv - Evolutionary Biology 2021Quote: ... the content of the wells was homogenised by pipetting up and down and 200 μL of suspension were transferred into a microplate-reader compatible plate (Fisherbrand 96-Well plates, Fisher Scientific, USA). The OD was measured at 600 nm (Infinite M200 PRO ...
-
bioRxiv - Immunology 2021Quote: ... 1×106 (24-well plate) or 5×105 (48-well plate) CD8+ T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher, 11456D) in a 1:1 bead to T cell ratio and cultured in 2 ml (24-well plate ...
-
bioRxiv - Immunology 2022Quote: ... The unlabeled tumor cells and transduced T cells were added to a 96 Well Black Plate glass bottom plate (Thermo Scientific, 160376). 60-70 z-stacks (0.2μm steps ...
-
bioRxiv - Systems Biology 2022Quote: ... cells were seeded at 8000 cells/well (96 well plate) or 35000 cells/well (24 well plate) in media without phenol red (Thermo Fisher 11054020) with glutamine supplemented (Life Technologies 35050-061) ...
-
bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
-
bioRxiv - Biochemistry 2024Quote: ... The Multiplate 96-Well PCR Plate is then incubated at 37 °C in a 96-well Non-Skirted PCR Plate Block (Thermo Scientific #88870120) in a digital dry bath (Thermo Scientific #88870006).
-
Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: dLN single-cell suspensions from C57BL/6 infected mice were cultured in 96 well plates “U” bottom with 5μg/mL of plate-bound anti-CD3 (clone 145-2C11, Invitrogen, Waltham, MA, USA), 0.5 μg/mL soluble anti-CD28 (clone 37.51 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: The assays were conducted in sterile Nunclon® 96 microwell plates and Nunc® 384 well polystyrene plates purchased from Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were transfected with 2ug per well (6 well plate) and 1ug per well (12 well plate) of plasmids concentration with the transfection reagent Lipofectamine 3000 (Invitrogen™, L3000001) and the protocol mentioned in the kit was followed ...
-
bioRxiv - Systems Biology 2023Quote: ... The 96-well plate was centrifuged to pellet cells and the pellets were pinned to a YPD + G418 + NAT plate (Nunc Omnitray #165218) and grown 2 days at 30°C to select for mated MATa/α diploids ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The phage kinetics were performed in flat bottom transparent 96 bacterial culture well-plates with lids (Thermo Scientific Optical-Bottom Plates, #265301) on a Synergy H1 multi-mode microplate reader (Agilent) ...
-
bioRxiv - Microbiology 2023Quote: ... pre-cultures were prepared by inoculating single colonies from a LB agar plate in 200 μl of liquid LB in a 96-well plate (Nunc, Thermo Scientific). After 22 h of growth at 37°C with 250 rpm shaking ...
-
bioRxiv - Microbiology 2023Quote: ... Formalin-killed N61 in PBS was dried in an oven at 5×106 cells/well in 96-well ELISA plates (C96 Maxisorpcert, Nunc-Immuno Plate, Thermo Fisher Scientific) overnight at 37°C ...
-
bioRxiv - Biophysics 2023Quote: ... Samples were added to a clear-bottom 96-well plate (Nunc MicroWell 96-Well Optical-Bottom Plate with Polymer Base, Thermo Fisher Scientific) (60 µL per well ...
-
bioRxiv - Microbiology 2023Quote: ... Biofilm samples were collected from agar plates gently with a spatula and then transferred to 24-well plates (Fisher Scientific, Cat.# FB012929). Before the phagocytosis assay ...
-
Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... CD8 T cells were cultured in 96 well plates “U” bottom with 5μg/mL of plate-bound anti-CD3 (clone 145-2C11, Invitrogen, Waltham, MA, USA), 0.5 μg/mL soluble anti-CD28 (clone 37.51 ...
-
bioRxiv - Biophysics 2021Quote: ... Secondary antibodies were goat anti-mouse-Star red (Abberior, 2-0002-011-2) and goat anti-rabbit Star 580 (Abberior 2-0012-0050-8) (Life Technologies, 1:100).
-
bioRxiv - Microbiology 2021Quote: ... a sequencing reaction was performed with 2 pmol of unmodified RNA and 2 µl of a 2 mM AS primer 1 labeled with Ned (Life Technologies SAS, France). Reverse transcription was performed as for the SHAPE (+ ...
-
bioRxiv - Neuroscience 2020Quote: ... and pMD2G—at a 2:2:1 ratio using Lipofectamine 2000 (Thermo-Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Candidate drugs were diluted 2-fold seriously by medium supplemented with 2% FBS (GIBCO), penicillin (100 IU/mL) ...
-
bioRxiv - Microbiology 2021Quote: ... (2) cells were incubated with 2 μg/mL BODIPY FL Vancomycin (Thermo Scientific, V34850); (3 ...
-
bioRxiv - Immunology 2020Quote: ... Washing medium consisted of 2% FCS and 2% P/S in RPMI 1640 (Gibco). FACS buffer contained 2% FCS and 2mM EDTA in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 μg/ml goat anti-rabbit F(ab’)2 fragment (Life Technologies A-21246) and goat anti-mouse F(ab’)2 fragment secondary antibodies were used ...
-
bioRxiv - Developmental Biology 2022Quote: Osteoblasts on glass coverslips were loaded with 2 µM Fura-2 AM-ester (Invitrogen) for 30 minutes at 25 °C in 107 mM NaCl ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were washed three times with 2× saline sodium citrate (2×SSC, ThermoFisher AM9765), and equilibrated with encoding-probe wash buffer (30% formamide (ThermoFisher AM9342 ...
-
bioRxiv - Cell Biology 2019Quote: ... pH 7.4) supplemented with 2 μM fura-2-AM (F1201, Molecular Probes, Eugene, OR) and 0.01 % pluronic acid ...