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Citations for Thermo Fisher :
6401 - 6450 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and reverse transcribed into cDNA using oligo dT primers and M-MLV Reverse Transcriptase (Invitrogen). Real-time PCR was performed on Quant Studio 6 Flex Real-time PCR system (Applied Biosystems ...
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bioRxiv - Pathology 2023Quote: ... KTN1 was amplified with primers harboring attB sites to facilitate Gateway cloning (Thermo Fisher Scientific). BP clonase II enzyme mix was used for a BP reaction into pDONR221 ...
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bioRxiv - Neuroscience 2022Quote: Random primers were used to construct a cDNA library from human foetal brain mRNA (Invitrogen) into the pP6 plasmid derived from the original pACT2 ...
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bioRxiv - Molecular Biology 2023Quote: ... reverse transcriptase and a combination of both random hexamers and oligo dT primers (Fisher Scientific). After reverse transcription ...
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bioRxiv - Biochemistry 2023Quote: ... mRNA (500 ng) was mixed with 1 μL of random primer solution (Thermo Fisher Scientific), to a final volume of 5 μL ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA synthesis was performed using SuperScript II with random primers (Invitrogen, Waltham, MA, USA). Quantitative real-time PCR was conducted on a StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µl primer and 10 µl TaqMan Universal Master Mix II no UNG (Thermofisher #4440040). TaqMan primer probes were purchased from Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... 4pmol of primer and a BigDye Terminator-ready reaction kit (Applied Biosystems, Foster City, USA). Cycle sequencing was carried out using both forward and reverse primer of each fragment in a GeneAmp9600 thermal cycler (Perkin Elmer ...
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bioRxiv - Immunology 2023Quote: ... 0.5 µL of Oligo dT primer (Invitrogen/Thermo Fisher, Cat. No. 18418012, Waltham, MA, USA), 0.5 µL of SUPERase-In (Ambion/Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... or custom primers with Power Track SYBR Green Master Mix (Thermo Fisher, Cat. No. A46012). See list of probes and primers for qRT-PCR and genotyping in Table S2.
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription was carried out using random hexamer primers and SuperScript III reverse transcriptase (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Fragment length analyses for all primers were conducted on ABI 3730 Genetic Analyzers (Applied Biosystems) at either the ETH Zurich Genetic Diversity Center (Switzerland) ...
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bioRxiv - Plant Biology 2023Quote: ... and reverse transcription was carried out using random hexamer primers and Maxima Reverse Transcriptase (Invitrogen). The ORNITHINE CARBAMOYLTRANSFERASE (OTC ...
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bioRxiv - Molecular Biology 2023Quote: ... An MGB probe of 15bp was designed using Primer Express software (Version 3.0.1; Applied Biosystems)TM ...
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bioRxiv - Molecular Biology 2023Quote: ... TaqMan MicroRNA Reverse Transcription Kit and gene specific stem-loop Reverse Transcription primers (Applied Biosystems) were used ...
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bioRxiv - Microbiology 2023Quote: Extracted RNA was converted into cDNA with an Oligo dT primer and Superscript III (Invitrogen). The 3’ half genome was then amplified with two rounds of PCR ...
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bioRxiv - Cell Biology 2023Quote: ... The primers of these genes were mixed with SYBR green reagent (Life Technologies, Warrington, UK) and quantitative RT-PCR was performed to quantify the expression level of the target genes ...
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bioRxiv - Immunology 2024Quote: ... 0.5 µM of primers and 1U of Platinum Taq High Fidelity DNA Polymerase (Invitrogen 11304102) in a final volume of 40ul ...
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bioRxiv - Microbiology 2024Quote: ... with specific primers and probes (Supplemental Table 1) and using a Quantstudio 3 (Applied Biosystems). The expressions of HCoV-229E M gene and SARS-CoV-2 E gene were quantified using a standard curve.
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bioRxiv - Physiology 2024Quote: ... cDNA was amplified using the appropriate primers and SYBR Green Master Mix (Thermo Fisher Scientific). Relative mRNA was calculated using the 2-ΔΔCt method after normalizing to actin and using fasted WT mice as the reference as previously described.27
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bioRxiv - Microbiology 2024Quote: ... smegmatis were amplified with specific primers (Table S1) using a thermocycler (MiniAmp Plus; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Primers and probes were designed and procured from Thermo Fisher (See Supplementary list star method). qRT-PCR analysis was then carried out using a TaqMan® RNA-to-CT™ 1-Step Kit 200 reaction and Applied Biosystems Real-Time PCR system on ABI quant studio ...
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bioRxiv - Plant Biology 2024Quote: ... cDNAs were synthesized using the oligo(dT) primer and RevertAid Reverse Transcriptase (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: cDNA was synthesized using the RevertAid FirstStrand cDNA Synthesis Kit with random hexamer primers (ThermoFisher) according to the manufacturer’s instructions and used in qPCR reactions with 2x Universal TaqMan Master Mix (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... and the extracted RNA was reverse-transcribed using Oligo(dT)18 primer (Thermo Fisher Scientific) and M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Microbiology 2024Quote: ... with 5 μg of total RNA from each sample and random primers (Invitro Life Technologies). cDNA was subsequently purified with the MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with Power SYBR Green PCR Master Mix (Applied Biosystems, Japan) using the Thermal Cycler Dice RealTime TP870 (Takara ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCRs (qPCR) were performed with SybrGreen dye in StepOnePlus PCR detector (Applied Biosystems). PCR amplification was performed for 40 cycles at 95 °C for 3 s and 60 °C for 30 s with a preceding initial enzyme activation of 20 s at 95 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative PCR analysis was performed using the 7500 Real-Time PCR system (Applied Biosystems) with Taqman 2x Fast universal PCR Master Mix (#4304437 ...
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bioRxiv - Zoology 2021Quote: ... Amplicon PCR reaction was performed using the GeneAmp PCR System 2700 (Thermo Fisher Scientific) and the following cycling conditions ...
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bioRxiv - Zoology 2021Quote: ... Amplicon PCR reaction was performed using the GeneAmp PCR System 9700 (Thermo Fisher Scientific) and the following cycling conditions ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was done using the ABI 7500 FAST qRT-PCR machine (Applied Biosystems) using the Taqman gene expression (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed with the 7500 Fast Real-Time PCR system (Applied Biosystems™) in duplicate of 20 μl reactions ...
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bioRxiv - Developmental Biology 2021Quote: ... then sequencing the PCR product after cloning via CloneJET PCR cloning kit (Thermo Scientific).
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were done using Phire Plant Direct PCR kit® (Thermo Scientific®) and gDNA from wings according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned using the ExoSAP-IT PCR Product Cleanup kit (Applied Biosystems) following the manufacturer’s protocol and then visualized using agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems; AB4367659). All measurements were performed in triplicate and were standardized to the levels of either HPRT or UBC ...
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bioRxiv - Genomics 2021Quote: ... Quantitative PCR was performed using the SYBR GREEN PCR Master Mix from Applied Biosystems. The target gene mRNA expression was normalized to the expression of two housekeeping genes (HPRT1 and RPL13) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The PCR products were purified with the Gene JET PCR purification kit (Thermo Fisher). Two hundred nanograms of PCR product were denatured and re-annealed in 10x NEB2 buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The final PCR products were purified by PureLink PCR purification kit (Thermo Fisher Scientific) and submitted for next-generation sequencing using Illumina MiSeq PE250.
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bioRxiv - Immunology 2021Quote: ... and qRT-PCR was performed on a StepOnePlus Real-time PCR system (Applied Biosystems) with TB Green Premix Ex Taq II (Takara Cat no.RR820A) ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed using SYBR™ Green PCR Master Mix (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was completed with Phusion High-Fidelity PCR Master Mix kit (Thermo Fisher, F531). At the same time ...
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bioRxiv - Molecular Biology 2020Quote: ... all PCR reactions were performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific). All mutants were verified by sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... PCR reactions were carried out with a 7500 Real-Time PCR System (Applied Biosystems) under standard conditions ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed by using 7900HT fast real time PCR system (Applied Biosystems). Expression of TFRC gene was normalized with actin.
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bioRxiv - Immunology 2022Quote: ... Real-time PCR was performed using SYBRgreen PCR Master Mix (Applied Biosystems, ThermoFisher Scientific) and the ViiA 7 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Real-time PCR was performed using SYBRgreen PCR Master Mix (Applied Biosystems, ThermoFisher Scientific) and the ViiA 7 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR amplification was performed on a GeneAmp® 9700 PCR System (Applied Biosystems). Cycling conditions consisted of an initial denaturation at 94°C for 3 min ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were cloned with a TOPO PCR cloning kit (Invitrogen Life Technologies) and sequenced (Eurofins) ...