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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set overnight and stained with antibodies against intracellular antigens (1 µg/ml) in Permeabilization buffer (ThermoFisher). For biotinylated antibodies ...
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bioRxiv - Molecular Biology 2021Quote: Samples were prepared at the EMBL-Heidelberg Proteomics Core Facility with the SP3 protocol71 and trypsin digested peptides analyzed by liquid chromatography followed by tandem mass tag (TMT)-labeled mass spectrometry using TMT10plex isobaric label reagent set (ThermoFisher). Raw data were mapped and quantified using MaxQuant (v1.6.3.4 ...
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bioRxiv - Neuroscience 2019Quote: ... This set-up was used to load the somatosensory cortex with the Ca2+-sensitive dye Oregon Green Bapta (OGB-AM; Invitrogen) and the astrocyte-specific marker sulforhodamine (SR101 ...
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bioRxiv - Synthetic Biology 2020Quote: ... A type IIS assembly reaction was set up into a 0.2 mL tube (5 µL nuclease-free H2O, 1 µL 10x Tango Buffer (Thermo Fisher), 0.5 µL mg/mL bovine serum albumin (NEB) ...
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bioRxiv - Cell Biology 2019Quote: IPHHs were thawed in a water bath set to 37°C and suspended in 10 ml Leibovitz’s L-15 Medium (Life Technologies) supplemented with Glutamax (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Surface-stained ESC-derived cells were fixed/permeabilized and stained using the eBioscience FOXP3/Transcription Factor Staining Buffer Set (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Supernatants from MSC and T-cell cocultures were analyzed using IL-10 Ready SET-Go! ELISA kits kits (Affymetrix, eBioscience) for secretion of IL-10 as previously described [31] ...
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bioRxiv - Neuroscience 2020Quote: ... sets of four frozen brains containing all treatment groups were embedded in NEG-50 (6502, Thermo Fisher Scientific, Waltham, MA). Brains were sectioned coronally in 30μm thick sections using a cryostat and mounted using a CryoJane tape transfer system (Leica Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: The components for reconstitution were set up in a slide-A-dialyzer (volume < 1 mL) (Thermo Fisher Singapore Pte Ltd.) or dialysis bag (volume > 1 ml ...
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bioRxiv - Synthetic Biology 2021Quote: ... Annealing reaction was set up by mixing 1.5 µL of each oligonucleotide (100 fM) with 5 µL T4-DNA ligase buffer (Thermo Scientific) in a total reaction volume of 50 µL ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from tissues using the Trizol Plus RNA Purification Kit with DNAse treatment (RNAse-Free DNAse Set, Ambion) and column cleanup (PureLink RNA Mini Kit ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of HIV-p24 and Ki67 was performed using the eBioscience Foxp3 / Transcription Factor Staining Buffer set (ThermoFisher Scientific). A list of flow cytometry panels and antibodies used is provided in S3 Table ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen and designed for the human sequences of cholesterol biosynthesis pathway genes ...
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bioRxiv - Cancer Biology 2020Quote: ... a pooled digest comprising aliquots of each sample set (180 μg) was desalted using Pierce desalting spin columns (Thermo Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2020Quote: The primer sequences are taken from PrimerBank (Wang and Seed, 2003) or were designed based on the probe set target sequence provided by Affymetrix using Primer3 software (www.genome.wi.mit.edu ...
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bioRxiv - Biophysics 2020Quote: Eight data sets and a total of 24,115 movies were collected manually or using EPU on various Titan Krios microscopes (Thermo Fisher) all equipped with energy filters (slit width of 20 eV ...
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bioRxiv - Developmental Biology 2022Quote: ... The fetal liver and lung were isolated from surrounding tissue by blunt dissection and set aside in a solution of 10% characterized fetal bovine serum in Hanks’ balanced salt solution (HBSS, Gibco). Using a 5mL syringe fitted with a 16-gauge needle ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher, #00-5523-00) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Gene expression values were then set equal to 2−ΔΔCt as described by the manufacturer of the kit (Applied Biosystems). All PCR primers were synthesized by Invitrogen with PPIA ...
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bioRxiv - Microbiology 2022Quote: ... The cells were placed onto 4 ml MSgg agar (1.5% w/v) set into each chamber of LabTek II 2-chamber microscope vessels (Thermo Scientific) set into various containers ...
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bioRxiv - Systems Biology 2019Quote: ... consisting of 43,856 probe sets that correspond to 13,358 mouse genes based on CHO EST Sequence and Annotation Release 06 (Affymetrix, Singapore). Quality assessment and data analysis were carried out on the CEL files obtained ...
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bioRxiv - Systems Biology 2019Quote: ... Labelled peptides from each set of replicates were then combined and cleaned up using SCX cartridge system (Applied Biosystems, USA) prior to 2D-LC-MS analysis.
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bioRxiv - Immunology 2020Quote: ... surface antigen-labeled cells were fixed and permeabilized with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) and incubated with the fluorochrome-labeled antibodies listed in Table S3 ...
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bioRxiv - Synthetic Biology 2021Quote: The 96 well qPCR reaction plates were set up using the Power SYBR Green qPCR mix (Thermo Fisher cat #4367659) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted from cells using the Trizol Plus RNA Purification Kit with DNAse treatment (RNAse-Free DNAse Set, Ambion) and column cleanup (PureLink RNA Mini Kit ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide extracted from each set were pooled and analyzed by nanoLC-MS/MS using an Ultimate 3000 system (Thermo Scientific) coupled to a TripleTOFTM 6600 mass spectrometer (AB Sciex) ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized after surface staining using Foxp3 Transcription Factor Staining Buffer Set (ThermoFisher, Cat#00-5523-00). Samples were acquired using LSRFortessa (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: Supported lipid bilayers were formed in 96-well glass bottom plates (Matrical) or set up on coverslips (25 mm diameter, No. 1.5 H, Marienfeld-Superior) mounted in Attofluor chamber (Thermo Fisher). 96-well plates were cleaned for 30 min with a 5% Hellmanex solution containing 10% isopropanol heated to 50°C ...
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bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The culture was split in half with one set being used for genomic DNA extraction while the other was used for total RNA extraction using TRIzol (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cell proliferation was determined by CFSE dilution and Treg differentiation was determined by surface staining of CD25 and intracellular staining of FOXP3 using the eBioscience Foxp3/Transcription Factor staining buffer set (Invitrogen). Data were collected using a FACSVerse flow cytometer using the BD FACSuite software and were analyzed with FlowJo 10.7.
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bioRxiv - Bioengineering 2022Quote: ... per the manufacturer’s protocols with the inclusion of an on-column DNase step using the PureLink DNase Set (ThermoFisher Scientific). At least eight replicates (3 µl droplets ...
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bioRxiv - Biophysics 2022Quote: ... were used to set up an overnight (16 hours) IVT reaction using mMESSAGE mMACHINE T7 ULTRA Transcription Kit (Invitrogen, AM1345). The overnight product was then treated with TURBO DNase ...
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bioRxiv - Cell Biology 2022Quote: ... si-Tomosyn-1 set 1(sense-GACCCAAAGCAGAAAGUUU, antisense-AAACUUUCUGCUUUGGGUC), and Tomosyn-1 set2 (sense-GAAAUUAAGCUUGCCAACU, antisense-AGUUGGCAAG CUUAAU UUC) using Lipofectamine 2000 (Invitrogen) at 1:2 ratio in Opti-MEM ...
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bioRxiv - Microbiology 2022Quote: ... Standard curve and extract reactions were set up in technical duplicate using TaqMan™ Environmental Master Mix 2.0 (ThermoFisher Scientific) and run on a StepOne Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... The concentration of protein in the RIPA buffer was determined with a Bradford assay and lysates were adjusted to a final concentration between 250-350 μg/μL depending on the yield of the lowest concentration of the lysate in the set in which it was processed with 1X LDS loading buffer (Invitrogen). RIPA soluble fractions were boiled for 5 minutes and stored at −80°C until use ...
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bioRxiv - Biophysics 2022Quote: ... supported lipid bilayer (SLB) was prepared upon 25 mm #1.5 glass coverslip set into Attofluor cell chamber (Invitrogen, Thermo Fisher). Coverslips were cleaned by sonication in 1:1 water:2-propanol then etched with piranha solution (1:3 mixture of 30% H2O2 and sulfuric acid) ...
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bioRxiv - Biophysics 2022Quote: ... supported lipid bilayer (SLB) was prepared upon 25 mm #1.5 glass coverslip set into Attofluor cell chamber (Invitrogen, Thermo Fisher). Coverslips were cleaned by sonication in 1:1 water:2-propanol then etched with piranha solution (1:3 mixture of 30% H2O2 and sulfuric acid) ...
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bioRxiv - Neuroscience 2024Quote: ... Positive control cells were set aside during the first stages of the reprogramming procedures (fig. S1, Cat Nº A16517, ThermoFisher).
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bioRxiv - Synthetic Biology 2024Quote: ... The annealing reactions were set up by mixing 1.5 µL of each oligonucleotide (100 µM) with 5 µL T4-DNA ligase buffer (Thermo Scientific) in a total reaction volume of 50 µL ...
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bioRxiv - Immunology 2024Quote: ... and lamina propria suspensions fixed for 10 minutes using the Foxp3/Transcription factor staining set (Thermo Fisher 00-5523-00). Cells were then resuspended in 1x permeabilization buffer (Thermo Fisher 00-8333-56 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Splenocytes were incubated at 4°C and washed three times with FACS buffer and fixed with FoxP3/Transcription Factor Staining Buffer Set (Invitrogen) fixative and stored at 4°C until intracellular staining ...
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bioRxiv - Microbiology 2024Quote: ... Samples were resuspended in 50 mM HEPES and labelled using the 0.5 mg TMTpro 18-plex Label Reagent Set (Thermo Scientific) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were first stained with extracellular markers and fixed and permeabilized using the eBioscience FOXP3/Transcription Factor Staining Buffer set (Thermofisher). Antibodies were then added according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated in a refrigerator set at °C with culture medium supplemented with 0.1M Hepes pH7.4 (Thermo Fisher Scientific). On day9 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were transferred to and kept in a refrigerator set at 4 °C with culture medium supplemented with 0.1M Hepes pH7.4 (Thermo Fisher Scientific).
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bioRxiv - Genetics 2024Quote: ... then fixed/ permeabilized for 1hr at 4°C using eBioscience™ Foxp3/ Transcription Factor Staining Buffer Set (Thermo Fisher Scientific). Cells were then washed with 1X permeabilization buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: TNF-α concentration in cell culture supernatants was determined using Ready-SET-Go! ELISA kits (ThermoFisher, Cat. 88-7324-88) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed and permeabilized using either the Foxp3/transcription factor staining buffer set (#00-5523-00, Fisher Scientific) or the BD fixation/permeabilization solution kit (#554714 ...