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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... TaqMan probes and primers for Ngn3 were assay-on-demand gene expression products (Applied Biosystems). All other primers (Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was reverse transcribed using oligo(dT)20 primers and SuperScript III (Invitrogen, Carlsbad, CA). Primers were designed using Eurofins Primer Design Tool ...
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bioRxiv - Systems Biology 2021Quote: ... The reactions were performed using unlabeled primers and SYBR Green DNA binding dye (Applied Biosystems) with a volume of 12 μL and the final primer concentrations of 0.4 μM ...
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bioRxiv - Plant Biology 2020Quote: ... 0.48 μL of 4 μM (universal) M13 primer labeled with VIC fluorescent dye (Applied Biosystems), 0.2 μL of 5.0 U μL−1 taq polymerase (Sigma Jumpstart™ ...
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bioRxiv - Cell Biology 2021Quote: ... and cDNA was synthesized using oligo dT primers first strand cDNA synthesis kit (Applied Biosystems). After standard curves were obtained ...
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bioRxiv - Developmental Biology 2022Quote: ... 500ng of RNA were reverse transcribed into cDNA using Random Primers and SuperScript II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.25 μL (5 μM) of each primer and 4.25 μL of ultrapure water (Gibco #10977). The cycling conditions included 50°C for 2 minutes ...
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bioRxiv - Genomics 2022Quote: ... excess primers and nucleotides were removed with 2.5 µl ExoSAP-IT (Thermo Fisher 75001.10.ML) and RNA was degraded with the addition of 1 µl 0.5 M EDTA and 3 µl 1M NaOH and heated at 70°C for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... qPCR was performed using gene-specific primers using PowerUp SYBR Green master mix (Applied Biosystems) and Bio-Rad CFX9 connect thermocycler (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... cell culturing reagents and primers and reagents used for qPCR were obtained from Life Technologies Inc ...
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of total RNA was reverse transcribed with random primers using SuperScript II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using SuperScript™ IV Reverse Transcriptase with Random Primers (both from Thermofisher) and quantified using PerfeCTa SYBR Green FastMix Low ROX (Quanta Biosciences).
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bioRxiv - Neuroscience 2020Quote: ... Uniplex qPCR was preformed using TaqMan Fast Advanced Master Mix and FAM labeled primers (ThermoFisher) with a ViiA 7 System (Applied BioSystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (ThermoFisher Scientific). Quantitative PCR was performed using the 5x HOT FIREPol® EvaGreen® qPCR Supermix (Solis Biodyne ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with oligo(dT) primers and the SuperScript™ II Kit (Thermo Fisher Scientific, Waltham, MA), following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... random hexamer primers were used to generate cDNA with a Verso kit from Thermo Scientific cat #AB1453/B ...
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bioRxiv - Physiology 2019Quote: ... Primers (Table S1) of oligo (dTs) with Super-Script III Frist Strand Synthesis SuperMix (Invitrogen) with s added to generate cDNA library (Hoege et al. ...
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bioRxiv - Physiology 2019Quote: ... RNA was converted into cDNA using oligo(dT) primer and the SuperScript IV kit (Invitrogen) or the qScript kit (QuantaBio) ...
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bioRxiv - Molecular Biology 2019Quote: ... and reverse transcribed into cDNA using gene-specific primers and reverse transcription kit (18090010, ThermoFisher). cDNA was analyzed by running PCR on a QuantStudio 7 Flex Real Time PCR System (Applied Biosystem) ...
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bioRxiv - Microbiology 2020Quote: ... using an oligo(dT)15 primer and SuperScript IV Reverse Transcriptase (18091050; Thermo Fisher Scientific). Because the major species of latency-associated transcripts (LAT ...
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bioRxiv - Pathology 2020Quote: ... followed by cDNA synthesis using a P7-containing primer and Superscript III Reverse Transcriptase (Invitrogen) and A-tailing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 1.0 μL of a 20× combined primers and probes mix (ABI/Life Technologies, USA). The cycle conditions were ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.0μl of 10uM of each primer and 16.05μl of 500ml DEPC-treated water (Invitrogen Corporation). PCR amplifications were performed using Applied Bio-systems thermo-cycler with a cycling profile of an initial step of 94°C for 2 minutes ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with primer pairs listed in Table S6 and PowerSYBR Green (Applied Biosystems) and analyzed with QuantStudio 5 (Thermo Scientific).
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bioRxiv - Genetics 2019Quote: ... as described16 with CRISPR-MoY-F and the invariant reverse primer (PAGE-purified, Life Technologies) (Supplementary Methods Table 1) ...
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bioRxiv - Systems Biology 2020Quote: ... oligonucleotides 1175 and 1176 as primers and Phusion High Fidelity Polymerase (Thermo Scientific, Pittsburgh, PA). DNA sequence analysis of the 5141 bp region encompassing the reporter gene cassette in the isolate designated pNC1152 using oligonucleotide primers M13R ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... cDNA was synthesized using random hexamer primers and the SuperScript III Reverse Transcriptase System (Invitrogen) following the manufacturer’s protocol ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... The TaqMan primers/probes for all the genes either purchased from Applied Biosystems (ThermoFisher Scientific) or commercially synthesized were listed in Supplemental Tables 1 and 2.
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bioRxiv - Physiology 2020Quote: ... mRNA reverse transcription was performed using oligo(dT) primers and Superscript II reverse transcriptase (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... RNA was reverse transcribed using Oligo(dT)12-18 primers (500 μg/ml; Invitrogen, UK), dNTP Mix (10 mM ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using the original reverse primer and BigDye® Direct Cycle Sequencing kit (Thermo Fisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... A list of the genes and the Assay ID for the primers obtained from ThermoFisher is provided in supplemental file S2 ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5μg of RNA were reverse transcribed using SuperScript IV Reverse Transcriptase and Random Primers (Invitrogen) for qPCR reactions.
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bioRxiv - Cell Biology 2021Quote: ... total RNA using oligo(dT) primers and SuperScript III First-Strand Synthesis System (Life Technologies). Quantitative RT-PCR reactions were performed using iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... TaqMan mRNA primer/probe assays and TaQman Gene Expression master mixes (both from Life Technologies) were used for semi-quantitative determination of genes of interest ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed using random hexamer primers with Maxima H Reverse Transcriptase (Thermo Fisher). Viral cDNA was then quantified via qPCR using primers complementary to spliced tat message and normalized to a housekeeping gene.
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bioRxiv - Cancer Biology 2022Quote: ... and reverse transcribed with random primers and the High Capacity cDNA Archive Kit (Applied Biosystems) as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was prepared using Oligo (dT18) Primer and RevertAid Reverse Transcriptase (Thermo Fisher Scientific, EP0441). Real-time PCR experiments were run on a Mastercycler (Biorad) ...
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bioRxiv - Immunology 2022Quote: ... primers and fluorescent probes included in the TaqMan Rodent GAPDH Control Reagents kit (Thermo Fisher) were used ...
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bioRxiv - Microbiology 2022Quote: ... determined with the same FAM-labeled primers and the Thermo Sequenase Dye kit (Thermofisher Scientific) was used to align the peaks ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were reverse transcribed with random hexamer primers using Multi-Scribe Reverse Transcriptase (Life Technologies). Real-time reverse transcription PCR was done with RPL27 as housekeeping gene ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA synthesis was performed using hexamer primers and SuperScript IV Reverse Transcriptase (Thermo Fisher 18090010). Processed samples were then subjected to RT-qPCR analysis using primer pairs targeting EP400 and ACTB for normalization.
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bioRxiv - Molecular Biology 2023Quote: ... cDNA synthesis was performed using hexamer primers and SuperScript IV Reverse Transcriptase (Thermo Fisher 18090010). Processed samples were then subjected to RT-qPCR analysis using primer pairs targeting Ep400 (Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primer assays for ADAMTS7 and RPLP0 as housekeeping gene (Hs00276223 and Hs00420895_gH, respectively; both ThermoFisher) were used to detect differences in gene expression between fibrous caps of stable and unstable plaques according to the histomorphologic AHA classification16 ...
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bioRxiv - Physiology 2022Quote: ... and reverse transcription was performed using oligo(dT)12-18 primers (18418012, Invitrogen, Waltham MA). Quantitative PCR reactions were performed using Power SYBR Green PCR Master Mix (4367659 ...
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bioRxiv - Immunology 2024Quote: ... 0.5 µM of primers and 1U of Platinum Taq High Fidelity DNA Polymerase (Invitrogen 11304102) in a final volume of 40ul ...
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bioRxiv - Cell Biology 2024Quote: ... First-strand cDNA synthesis was performed using 500 ng of RNA with random primers (ThermoFisher) and MMLV reverse transcriptase (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: ... using described primers (Table S2) followed by re-circularisation or insertion using T4 ligase (Invitrogen). All constructs were generated using blunt-end cloning ...
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bioRxiv - Immunology 2024Quote: ... with primers containing attB sites (Table 1) and Phusion Plus DNA Polymerase (Thermo Fisher, F530N). OVA entry fragment was cloned into donor vector pDONR221 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was amplified using primers from IDT and PowerUp SYBR Green master mix (Thermo Fisher) as per manufactures instructions.