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Citations for Thermo Fisher :
6201 - 6250 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were co-transfected with 300ng of Cav1.2-HA at DIV6 and 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher), and processed for immunostaining 13 days after ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were transfected at DIV 5 with 100ng of GFP alone or co-transfected with 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher). After 5 days of expression ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were co-transfected at DIV 5 with 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p or Neg Control 1, Thermo Fisher) and 100ng of the Cacnb2 3’UTR luciferase reporters ...
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bioRxiv - Genetics 2021Quote: ... The quantitative RT-RCR analysis were performed using SYMR Green fluorochrome technique on a PikoReal 96 Real-time Thermal Cycler (Thermo Fisher Scientific, Shanghai, China) under the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... and SYBR Green Real-Time qPCR reagents (Thermo Fisher). Reactions were run in a Lightcycler 96 system (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time RTPCR was conducted using a QuantStudio3 (ThermoFisher) for all experiments ...
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bioRxiv - Microbiology 2020Quote: ... on an ABI 7900HT real-time cycler (Applied Biosystems). Sequencing was performed in Illumina MiSeq with 2 × 300 bp reads using v3 chemistry with a loading concentration of 18 pM ...
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bioRxiv - Neuroscience 2020Quote: ... on a StepOnePlus Real Time System (ThermoFisher, Waltham, MA). Libraries were mixed to run four samples per lane on the HiSeq 2500 (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... on a StepOnePlus real-time qPCR machine (Applied Biosystems). The data were exported MATLAB and analysed in GraphPad ...
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bioRxiv - Molecular Biology 2019Quote: ... using a 7900HT Fast Real-Time System (Applied Biosystems). The qPCR was carried out as follows ...
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bioRxiv - Microbiology 2019Quote: ... and SYBR Green Real-Time qPCR reagents (Thermo Fisher) with an annealing temperature of 55°C ...
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bioRxiv - Genetics 2021Quote: ... in a StepOne Plus real-time thermocycler (Applied Biosystems). The gene of interest and the reference gene were detected simultaneously in each sample ...
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bioRxiv - Microbiology 2020Quote: ... using a ViiA7 Real-Time qPCR system (Applied Biosystems). Primer sequences are provided in Key Resources Table ...
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bioRxiv - Developmental Biology 2020Quote: ... on a StepONE PLUS real-time thermocycler (Applied Biosystems). PCR primers were designed based on the 4 zebrafish stc gene sequences obtained from the Ensemble database (Ensemble gene numbers are ...
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bioRxiv - Immunology 2022Quote: ... using a ViiA7 Real-Time qPCR system (Applied Biosystems). Test genes in C ...
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bioRxiv - Neuroscience 2022Quote: ... on a Real Time qPCR machine (Applied Biosystems 7900HT). Relative transcript abundance was estimated using the ΔΔCt method ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Real time analysis was performed using Sybr Green (Invitrogen) reagent in ABI HT 4900 machine according the manufacturer’s instructions and with 58°C as the annealing temperature ...
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bioRxiv - Microbiology 2023Quote: ... on an ABI 7900HT real-time cycler (Applied Biosystems). Sequencing was performed on an Illumina MiSeq with 2 × 300 bp reads using v3 chemistry with a loading concentration of 18 pM ...
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bioRxiv - Immunology 2023Quote: ... through ViiA-7 Fast Real-Time System (Applied Biosystems). Data were processed using ViiA™ 7 Software (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... hsa-let-7a (Thermo Fisher, assay id 000377), hsa-let-7b (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... hsa-let-7i (Thermo Fisher, assay id 002221), hsa-miR-100 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... hsa-let-7b (Thermo Fisher, assay id 002619), hsa-let-7i (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was then performed using AgPath RT-PCR (Life Technologies) kit on a QuantStudio(TM ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using QuantStudio 3 PCR system (Applied Biosystems) or StepOnePlus Real-Time PCR System (Applied Biosystems).
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bioRxiv - Molecular Biology 2024Quote: ... or in RT-PCR reactions with 2x PCR Master Mix (ThermoFisher). The expression of TTN ...
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bioRxiv - Developmental Biology 2023Quote: ... a 10-min incubation at RT with streptavidin associated with peroxidase (UltraVision Detection System HRP kit, Thermo Fisher Scientific) was performed ...
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bioRxiv - Cell Biology 2019Quote: ... mRNA knockdown was determined by RT-qPCR (Applied Biosystems 7500 Fast RT-PCR) using 2 independent primer sets for each transcript with Hprt1 and cyclophilin B (Ppib2 ...
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bioRxiv - Cell Biology 2020Quote: ... using one step TaqMan RT enzyme and TaqMan RT-PCR Mix (Applied Biosystems). Relative quantification from triplicate amplifications were calculated by normalizing averaged threshold cycle (Ct ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-qPCR analysis was performed using Power SYBR™ Green RNA to CT™ 1 step RT-PCR kit (Applied Biosystems, #4389986) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-qPCR was performed using the RT-PCR Kit (Seven, Beijing, China) on the Quant Studio 3 (Thermo Fisher Scientific, Massachusetts, USA), according to the manufacturer’s procedures ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions were placed in 96-well plates and read with the Fast 7500/7500 Real-Time PCR System (Applied Biosystems). Data analysis was performed using the Fast 7500/7500 RealTime PCR System built-in software (Applied Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR reactions were performed using power SYBR green master mix on a StepOnePlus real-time PCR system (Applied Biosystems). Relative gene expression levels were normalized to GAPDH.
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bioRxiv - Cell Biology 2020Quote: ... The target genes were amplified and detected with an Applied Biosystems QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) and the gene expression levels were normalized to β-Actin ...
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bioRxiv - Genetics 2021Quote: ... DSF was performed by heating samples containing 10X SYPRO Orange in 1°C increments from 25°C to 100°C using the StepOne real-time PCR machine and using the Protein Thermal Shift software (ThermoFisher) to fit the data.
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bioRxiv - Genetics 2021Quote: ... was used to quantify extracts according to the manufacturer’s recommended protocols using the Applied Biosystems’ 7500 real-time PCR instrument (ThermoFisher Scientific).
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bioRxiv - Genetics 2020Quote: ... 10 ng cDNA was used as the template for each real-time PCR reaction by SYBR Green mix (Thermo Scientific) with the Applied Biosystems 7300 ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin-immunoprecipitated DNA was quantified by qPCR using SYBR Green and a 7900HT Fast Real-Time PCR machine (Applied Biosystems) and primers specific for Eif4ebp1 ...
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bioRxiv - Developmental Biology 2020Quote: ... and carried out for three biological replicates with measurements taken from three technical replicates on an Applied Biosystems 7300 Real Time PCR system (Thermofisher). Mutants and siblings were then sorted by comparing their amplification curves upon qPCR with the WT and mutant set of primers (Ctmutant – CtWT).
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng template was used per reaction with SYBR Green Mastermix in ViiA 7 Real-Time PCR System (Applied Biosystems). As endogenous control ...
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bioRxiv - Developmental Biology 2021Quote: ... Real time qPCR was carried out for three biological replicates with measurements taken from three technical replicates on an Applied Biosystems 7300 Real Time PCR system (Thermofisher). After normalization with zebrafish elongation factor 1a (ef1a) ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Relative mRNA expression was quantified using 4 ng of cDNA on a StepOne Plus Real-Time PCR System (Life Technologies) and the ΔΔCT method [45] ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was then performed for at least 3 biological replicates using Biozym Blue S’Green qPCR Mix Separate ROX (No. 331416XL) on QuantStudio 7 Flex Real-Time PCR System from Applied Biosystems. Pax3 fold change was calculated from ΔCt using Gapdh as housekeeping gene (2^-(ΔCt)) ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR reactions were performed in triplicate for each Taqman assay of interest on a QuantStudio 3 Real Time PCR Machine (ThermoFisher).
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bioRxiv - Genomics 2020Quote: ... qPCR was performed in duplicates in 384-well optical plates in a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) using default settings ...
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bioRxiv - Immunology 2021Quote: Multiplex quantitative polymerase chain reaction (qPCR) was carried out using a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) as described previously [23] ...
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bioRxiv - Microbiology 2019Quote: ... Viral titers were determined using the SuperScript® III Platinum® One-Step Quantitative real-time PCR system (Life Technologies) with the following probe and primers ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were run with 3 technical replicates each on a ViiA7 Real-time PCR system (Thermo Fisher, Waltham, MA, U.S.A.) programmed as follows ...
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bioRxiv - Neuroscience 2019Quote: ... and 0.5 μM of gene-specific primers (Table S2) in a 7500 Fast Real-Time PCR System (Thermo Fisher Scientific), using a two-step cycling protocol (3 seconds at 95°C ...
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bioRxiv - Zoology 2019Quote: Gene expression levels were analysed with quantitative polymerase chain reaction (qPCR) using a StepOnePlus Real-Time PCR System (Applied Biosystems) as described previously [25] ...
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bioRxiv - Physiology 2020Quote: ... Measurements of mRNA were performed with SYBR green chemistry using the QuantStudio 6-flex Real-time PCR System (ThermoFisher Scientific). All data were normalized to either Hprt or Actinb by the ΔC(t ...