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Citations for Thermo Fisher :
6201 - 6250 of 7943 citations for Recombinant Human Leptin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: MS/MS spectra were searched against human database from Uniprot (downloaded on 04/2015) with Proteome Discoverer 2.1.1.21 (ThermoFisher Scientific). Methionine oxidation (+15.9949 Da) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA extraction was performed from the medial layer of the aorta and subsequently sequenced by HTA 2.0 (Human Transcriptome Array 2.0 - Affymetrix) platform.
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bioRxiv - Developmental Biology 2023Quote: Human ESCs were thawed and maintained on mitotically inactivated MEFs in Knock-Out DMEM media (Thermo Fisher Scientific) supplemented with 20% Knock-Out Serum Replacement (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... Human GM12878 lymphoblastoid cell line (Coriell Institute) which has a relatively normal karyotype was cultured in RPMI (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... IL-1β secretion from PMA-THP1 was detected using IL-1 beta Human ELISA kit (#KHC0011, ThermoFisher Scientific) and IL-1 beta Human ELISA kit ...
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bioRxiv - Microbiology 2023Quote: ... Human embryonic kidney 293T cells (HEK 293T) were maintained in DMEM GlutaMAX (Dulbecco’s Modified Eagle Medium, ThermoFisher Scientific) supplemented with decomplemented FCS 10% (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Human colon adenocarcinoma HT-29 cells were routinely cultured in Dulbecco’s Modified Eagle’s Medium (Cellutron Life Technologies, USA) with a high glucose concentration ...
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bioRxiv - Bioengineering 2023Quote: ... The plasma insulin concentration was determined using a Human Insulin ELISA kit according to the manufacturer’s protocol (Invitrogen). The relative bioavailability (RA ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were then transfected into human embryonic kidney cells (HEK293-E, National Research Council, Canada) using 293Fectin (Thermofisher). Six days post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Human OTUD4 constructs were cloned without tag or with FLAG-tag into the expression plasmid pcDNA3.1 (Life technologies) using the oligos forward (BamHI ...
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bioRxiv - Cancer Biology 2023Quote: Human osteosarcoma cell line U2OS (ATCC) and COV362 (ATCC) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM; Gibco) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: Human umbilical vein endothelial cells (HUVECs) were isolated and cultured in an M199 medium (Thermo Fisher Scientific, 31100035) supplemented with fibroblast growth factor ...
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bioRxiv - Molecular Biology 2023Quote: ... The human cell lines were cultured in DMEM/High glucose medium (Cat # SH30243.02, Thermo Fisher Scientific, Logan, UT) supplemented with 10% FBS (Cat # F2442 ...
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bioRxiv - Microbiology 2022Quote: ... TNF-alpha treatments were performed for 45 min using 25 ng/ml of human TNF-alpha (Thermo Fisher). Cells were permeabilized with 0.1% Triton X in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2023Quote: Human RPE-1 cells were cultured in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F- 12 (DMEM/F12) (GIBCO), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Combined libraries were dried down with universal blockers and human cot DNA in a Savant SpeedVac (ThermoFisher Scientific) vacuum concentrator ...
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Neuroscience 2024Quote: Human CSF2RB WT (exons 11 to 14) and A455D mutant donor targeting sequences were cloned into pDONR221 (ThermoFisher) with the following layout (Supplementary Figure S1) ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 x 5 min with PBS and incubated with Alexa Fluor-488 goat anti human secondary (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco by Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were activated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco by Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2023Quote: ... Huh7 (Human hepatocellular carcinoma) cells were grown in DMEM (Dulbecco’s Modified Eagle Medium) (Gibco; 11885-084; Low glucose), and HepG2 (Human hepatocellular carcinoma ...
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bioRxiv - Immunology 2023Quote: ... human samples were stained with eBioscience™ Fixability Viability Dye eFluor™ 780 (65-0865-18, ThermoFisher, Canada), while murine samples were stained with Zombie Green™ Fixable Viability Dye (423111) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the top 14-HAP depleted and fractionated sample were searched for peptides through the human UniProtKB/SwissProt database (v2021-11-30, 20,306 canonical sequences) using Proteome Discoverer (version 2.5.0.400, Thermo Fisher Scientific). The parameters applied to the search engines Sequest HT and Mascot were set as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... MCF10A (mammary gland epithelial; human female origin, ATCC #CRL-10317) cells were maintained in DMEM/F12 (Life Technologies) supplemented with 5% horse serum ...
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bioRxiv - Bioengineering 2023Quote: ... hNPCs: Primary human nucleus pulposus cells (hNPCs) were cultured in complete growth medium containing DMEM-HG (ThermoFisher Scientific) supplemented with 10% FBS (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... 77,897 human protein sequences) using the Sequest search engines within the Proteome Discoverer platform (version 2.4, Thermo Scientific). The following search parameters were used ...
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bioRxiv - Biochemistry 2023Quote: HeLa cells (human cervical adenocarcinoma) were acquired from ATCC (#CCL-2) and maintained in DMEM medium (Gibco #10569010) supplemented with 10% fetal bovine serum (Gibco #10437028 ...
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bioRxiv - Cancer Biology 2023Quote: ... Adherent human glioblastoma cell lines and patient-derived cell cultures were passaged using Trypsin-EDTA (0.25%, Gibco, #25200056). For DRUG-seq ...
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bioRxiv - Microbiology 2023Quote: ... while uninfected bovine macrophages (BoMac)64 and human embryonic kidney cells (HEK293FT) were grown in DMEM medium (Gibco). HAP1 cells ...
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bioRxiv - Developmental Biology 2023Quote: The human Ishikawa were grown in flasks in Modified Eagle’s Medium (MEM, Gibco, Thermo Fisher Scientific, ref. 10370021), supplemented with 5% fetal bovine serum (Biowest ...
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bioRxiv - Physiology 2024Quote: ... Total RNA Microarray analysis was performed with the Affymetrix GeneChip Human Transcriptome Array 2.0 (Thermo Fisher Scientific, MA). Metabolomic analysis was performed by Metabolon (Durham ...
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bioRxiv - Microbiology 2024Quote: Human bladder epithelial cell line 5637 (RRID: CVCL_0126) were cultured and maintained in RPMI medium (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS (Sigma ...
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bioRxiv - Microbiology 2024Quote: Human bladder epithelial cell line 5637 (RRID: CVCL_0126) were cultured and maintained in RPMI medium (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... and then treated with Alexa Fluor 488-conjugated goat anti-human IgG (catalogue no. A11013; Thermo Fisher Scientific) at a concentration of 2 μg/ml in DMEM with 2% FBS for 30 minutes (min ...
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bioRxiv - Immunology 2023Quote: ... post-dose 3 and 6-months post-dose 3 using mouse anti-human IgG1 biotin (Thermo Fisher Scientific) and mouse anti-human IgG4 biotin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... the ELISA plates were incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (1:5000, Invitrogen) for 1 hour at 37°C ...
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bioRxiv - Physiology 2024Quote: Amyloid-β 40 (Aβ40) in the brain was quantified using a human Aβ40 ELISA kit (Invitrogen, USA, #KHB3482) in accordance with manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The human monocytic THP-1 cell line (from ATCC) was cultured in RPMI1640 medium (Thermo Fisher Scientific Inc.) supplemented with 10% FBS and stimulated with OxLDL1 and OxLDL2 at a concentration of 20 µg/mL.
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bioRxiv - Immunology 2024Quote: NK92 cells expressing human FcγRIIIa (CD16.NK-92) (ATCC) were maintained in α-MEM without nucleosides (Thermo Fisher), 2mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney HEK293T (ATCC, catalog #: CRL-3216) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2024Quote: Human embryonic kidney (HEK)-293T cells (ATCC CRL-3216) were cultured in 10% FBS DMEM (Gibco 11965-092), supplemented with penicillin (50 U per mL ...
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bioRxiv - Immunology 2024Quote: ... IL-18 and human IL-1β was analyzed in the cell culture supernatant by commercial ELISA kits (Invitrogen) following the manufacturer’s recommended procedures.
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bioRxiv - Immunology 2024Quote: ... Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco, A29133). IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: Human cervical adenocarcinoma cells (HeLa) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with GlutaMAX™ (Gibco, ThermoFisher) supplemented with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... plated at 80,000 cells/chamber and chemoattracted to growth medium containing 50 nM of human HGF (Life Technologies). Drugs were added in transwell assays at the time of cell seeding ...
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bioRxiv - Microbiology 2024Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, Gibco, 12800082) supplemented with 10% FBS (Pan Biotech ...
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney HEK293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, Gibco, 12800082) supplemented with 10% FBS (Pan Biotech ...
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bioRxiv - Cancer Biology 2024Quote: Primary Human Hepatocyte: Cytosolic and nuclear fractions were prepared using the NE-PER cell fractionation kit (Thermo Fisher) according to the manufacturer’s instruction ...