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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Samples were boiled for 5 min prior to loading 7 µg of samples on to 4-15% SDS-PAGE gels alongside a molecular weight ladder (Thermo Fisher Scientific, cat# LC5602) and electrophoresis at 200V for 40 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tris-Glycine 10-20% or Bis-Tris 4-12% SDS-PAGE gels were run at 4℃ and proteins were transferred onto nitrocellulose membranes using 15 Volts for 7 minutes via the iBlot-2 system (Thermo Fisher Scientific, Waltham, MA). The membrane was then blocked for 1 hour in LICOR Odyssey blocking buffer and incubated at 4℃ with the appropriate antibody overnight ...
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bioRxiv - Microbiology 2021Quote: ... TaqMan analysis was carried out with primer/probe combination described in Table 1 and analysis performed on the QuantStudio™ 6 Flex System (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:9 ratio (in 6-well plates at 60-70% confluency) using Lipofectamine®LTX with Plus™ Reagent (Invitrogen) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RT-PCR products were fractioned by electrophoresis using 6% polyacrylamide gels in 1 x TBE and Sybr safe staining (Thermo scientific, S33102). The bands corresponding to exon inclusion or skipping were quantified using ImageJ 1.47v (NIH ...
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bioRxiv - Immunology 2019Quote: ... Embryo carcasses were placed into individual 6 cm2 tissue culture plates containing 1 mL of pre-warmed 37°C TrypLE (Thermo Fisher, 12604013) and were incubated at 37°C for 20 min ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... 6.104 HL-1 cells were plated into each well of a 6-well plate and transduced overnight in 1 mL of transduction medium (OptiMEM-GlutaMAX, Life Technologies SAS) containing 5 µg/mL protamine sulfate in the presence of lentivectors (MOI 2) ...
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bioRxiv - Neuroscience 2020Quote: ... and placed in a 6-well plate filled with 1 ml/well of culture media containing 50 % Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23 % EBSS ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Cell Biology 2021Quote: ... HSC (Lin−Sca-1+c-Kit+CD150+CD48−) from wildtype C57BL/6 mice were sorted into 16-well chamber slides (Thermo Fisher Scientific) coated with RetroNectin (Takara Bio) ...
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bioRxiv - Neuroscience 2022Quote: ... prior to washing (6×60 min PBST) and secondary incubation with species specific Alexafluor IgG secondary antibodies (60 min RT, Invitrogen; 1:500). Tissues were washed again in 0.1% PBST (6 × 60 min) ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Virus mutant libraries were rescued in a co-culture of HEK 293T and MDCK-SIAT1 cells (ratio of 6:1) at 60% confluence in a T75 flask (75 cm2) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: sg-mEX3 or sg-mEX5 and SpCas9-2A-GFP plasmids at 1:1 molar ratio were co-transfected into ~80% confluence OC-k1 cells in 6-well plate using Lipofectamine 3000 (Thermo Fisher Scientific). The cells were incubated for 48 hours and then submitted for fluorescence-activated cell sorting ...
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bioRxiv - Genomics 2021Quote: ... 4*105 TX1072 cells cultured in SL medium were reverse transfected with 250 ng pX330-PK45/46 and 750 ng of the pA cassette-containing donor plasmid (SP292) with Lipofectamine 3000 reagent using 1 µl P3000 reagent and 6 µl Lipofectamine 3000 (Thermo Fisher Scientific) following the manufacturer’s guidelines ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... formation was induced by culturing cell aggregates in suspension from 1-well of a 6-well plate on a 35×10mm petri dish (Thermo Fisher Scientific) with mTeSR1 medium ...
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bioRxiv - Genetics 2020Quote: ... Confocal microscopy was carried out embedding the 6 dpf larvae in 1 % (w/v in E2 medium containing tricaine) low-melting agarose (Fisher Scientific, USA) in a 50-mm glass bottom dish (MatTek ...
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bioRxiv - Biophysics 2022Quote: ... 1 x 106 Sf9 cells were transferred to each well of a six-wells plate and subsequently transfected with 1 μg of bacmid DNA by 6 μl of Cell-fectin (Thermo Fisher Scientific). Baculovirus containing cell supernatants (P1 ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with 1 µg of the indicated plasmids (6-well plates) by using the Lipofectamine 2000 system (Thermo Fisher Scientific). For transient transfection with siRNA ...
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bioRxiv - Microbiology 2023Quote: ... or macroH2A-3Xflag (6 x 106 on 100 mm dish) were cross-linked with 1 % of methanol-free formaldehyde (Thermo Scientific, 28906) in DMEM for 10 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The pellets were resuspended in 4.5 mL distilled water to which is added 0.5 mL 6% hypochlorite solution (Fisher Scientific Cat# SS290-1) and gently rocked for 1 minute followed by centrifugation (800g for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 6×10 minutes in .05M PBS and incubated with both Alexa-Fluor 488 and 555 secondary antibody (1:1000; Thermo Fisher Scientific) in .05M PBS with .1% Triton x100 for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected for 6 or 24 hours with Imp1-targeting siRNAs (siRNA #1: s155193, or siRNA #2: s155193. Both from Ambion, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... 10 x 1 µL droplets of each diluted suspension were deposited on polystyrene material (6-well plates, Thermo Scientific, Cat. No. 140675) and exposed to ambient temperature and variable RH (20% ...
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bioRxiv - Cell Biology 2023Quote: ... was used to amplify the genes of interest according to the primers mentioned in the Table 1 from 15-20 ng of cDNA in the QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific) machine ...
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bioRxiv - Neuroscience 2023Quote: ... and placed in a 6-well cell culture plate filled with 1 ml/well of culture media containing 50% Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23% Earle’s Balanced Salt Solution ...
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bioRxiv - Cell Biology 2023Quote: ... plated at a density of 1 X 106 cells/well in a Matrigel-coated 6 well plates (BD, 354277; Fisher Scientific, 140675). For the first 24 hours after passaging ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Microbiology 2023Quote: Mammalian cells were seeded at a density of 1×106 in a 6-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cell Biology 2023Quote: ... 2 x 105 cells were seeded in a 6-well plate containing coverslips and transfected with 1 µg dsRNA mixed with Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio ...
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bioRxiv - Immunology 2024Quote: MonoMac-6 effector cells were stained with 1 μM of CellTrace™ Far Red dye (CTFR, cat. no. C34564, Thermo Fisher Scientific) in PBS (cat ...
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bioRxiv - Genetics 2024Quote: ... RT-PCR products were fractionated by electrophoresis using 6% polyacrylamide gels in 1 x TBE and Sybr safe staining (ThermoFisher Scientific, S33102). The bands corresponding to exon inclusion or skipping were quantified using ImageJ v1.47 (NIH ...
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bioRxiv - Cell Biology 2024Quote: ... 1.7 x 105 Huh7 cells were seeded in a 6-well plate and transfected for 16 h with 1 µg purified dsRNA mixed with Lipofectamin 2000 transfection reagent (Thermo Fisher Scientific) in a 1:3 ratio prior to actinomycin D chase.
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bioRxiv - Plant Biology 2024Quote: ... were used to amplify a 1:16 dilution of cDNA in triplicate in a Quantstudio™6 PRO real-time thermal cycler (ThermoFisher, Inc.) with a 61°C annealing temperature ...
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bioRxiv - Neuroscience 2024Quote: ... into the medial septal area (MS) of C57Bl/6NTac mice (n=6). For the colocalization experiments (Fig. 1) we injected yellow-green FluoSpheres (Thermo Fisher Scientific) into the MS of two C57Bl/6NTac mice or AAVrg-syn-FLEX-jGCaMP8m-WPRE (Addgene ...
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bioRxiv - Neuroscience 2020Quote: ... Ganglia were dissociated by trituration using a fire polished Pasteur pipette, followed by centrifugation (5 minutes, 160 x g) and trituration in wash buffer (DMEM Ham’s F-12, Gibco, 10565-018 ...
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bioRxiv - Neuroscience 2020Quote: Time plug-mated Pbx3−/- mice received a single intraperitoneal injection of 5-ethynyl-2’-deoxyuridine (EdU; 0,1mg/g animal; Invitrogen) at E15.5 ...
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bioRxiv - Cell Biology 2022Quote: ... non-IR and IR animals were systemically injected with 0.9% saline containing 0.25 mg per 25 g of body weight of 5-ethynyl-2’-deoxyuridine (EdU, Thermofisher Scientific) followed by a 2 hour or 7 days chase before sacrifice ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... Tissues were centrifuged at 200 g for 5 min and washed with Hank’s Balanced Salt Solution (Thermo Fisher Scientific). Tissues were then digested with 0.25% trypsin/EDTA and DNase I for 8 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were pelleted at 300 x g for 5 minutes and resuspended in freezing medium (fetal bovine serum (Gibco), 10% v/v DMSO) ...
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bioRxiv - Microbiology 2020Quote: Samples consisting of either solubilized membranes or purified membrane complexes were mixed with 5% G-250 sample additive (Invitrogen), to a final concentration of ~0.2% ...
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bioRxiv - Immunology 2021Quote: ... Remaining cells were centrifuged for 5 minutes at 500 g before 100µL of extraction buffer added (Arcturus Picopure RNA kit, Thermofisher) and lysates frozen at −80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were cultured at 37°C with 5% CO2 in 4.5 g/L glucose DMEM (ThermoFisher, Waltham, MA, #10566016) supplemented with 10% Fetal Bovine Serum (HyClone ...
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bioRxiv - Biochemistry 2022Quote: ... The remainder of the lysate was added to 5 µL of Pierce Protein G agarose beads (Thermo Fisher Scientific), and the mixture was rotated 1-2 hours at 4°C to pre-clear ...
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bioRxiv - Bioengineering 2022Quote: ... the EBs were transferred to StemPro-34 with rhVEGF (5□ng/ml) for another five days and then to DMEM high glucose (4.5□g/l, ThermoFisher) media with compaction factors (CHIR (1□uM ...
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bioRxiv - Cell Biology 2022Quote: ... were maintained as a confluent culture at 37°C and 5% CO2 in DMEM (4.5 g/L glucose, Gibco) supplemented with 10% heat- inactivated FCS ...
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bioRxiv - Neuroscience 2024Quote: ... followed by centrifugation (5 minutes, 160 x g) and two rounds of trituration in wash buffer (DMEM Ham’s F-12, Gibco 10565-018 ...
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bioRxiv - Genomics 2024Quote: ... 5-10 μg of antibody was coupled to 50-100 μl of Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were maintained in high glucose Dulbecco’s modified Eagle’s medium 5 g/L glucose (DMEM; Gibco, UK, 11965084). Cell culture media were supplemented with 10% (v/v ...