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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and TRH neurons with rabbit proTRH antibodies (PA5-57331, Invitrogen, Waltham, MA, USA; 1:1,000; 48h; 4 °C) (50) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were fixed with 4% PFA and immunostained against Tuj1 and Synaptophysin (1:1000, Invitrogen, cat# PA1-1043). Images acquired using confocal microscopy and total area of synaptophysin positive puncta was measured and normalized to Tuj1 total area.
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated in primary antibody at 4°C overnight (biotinylated AT8, 1:500, MN1020B, ThermoFisher Scientific). The biotin signal was amplified using an ABC Elite kit (1:400 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then immunostained overnight at 4°C with primary antibodies (SOX2 (1:1200, Invitrogen #14-9811-82) and BRA (1:400 ...
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bioRxiv - Cancer Biology 2022Quote: ... overnight at 4 °C and followed by staining with FITC- streptavidin (Invitrogen, 11-4317-87, 1:400 dilution) for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The coverslips were then incubated for 10 min at room temperature in 1 mL 4% formaldehyde (Thermo Scientific) in PBS and washed 3 times in PBS.
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bioRxiv - Developmental Biology 2023Quote: ... Gonads were washed 4×20min in PBT and then incubated in PBT containing DAPI (1:1000, Molecular Probes), with or without DiOC6 (1:5000 ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed 4 times with wash buffer before being developed with 1-Step PNPP substrate (ThermoFisher) for ∼ 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... MDCK cells were plated for 24hr on LabTek #1 4-well chamber slide (43300-776, Thermo Fisher Scientific), wounded with vacuum suction P200 micropipette tips and recovered for 2hrs ...
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bioRxiv - Developmental Biology 2023Quote: ... Following another 48 h of incubation at 4°C with secondary Alexa Fluor antibodies (1:100–200; Invitrogen), samples were cleared overnight in Refractive Index Matching Solution (RIMS ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 4 mM L-alanyl-L-glutamine and NucBlue nucleus staining reagent (1 drop per mL) (Invitrogen). Plates were incubated for 15 min (37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 4 mM L-alanyl-L-glutamine and NucBlue Live ReadyProbes Reagent (1 drop per mL) (Invitrogen). Plates were incubated for an additional 10 min (37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were stained overnight in 4 °C with rat-anti-CD4 (1:100, Invitrogen, 14-9766-82) and rabbit-anti-Iba1 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and incubated overnight at 4°C with anti-ChAT (choline acetyltransferase) goat polyclonal antibody (1:500, Invitrogen #PIPA518518) and anti-c-Fos rabbit polyclonal antibody (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 4◦C with either anti-ZO1 (1:100; Clone ZO1-1A12, ThermoFisher Scientific) or anti-CK19 (1:400 ...
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bioRxiv - Developmental Biology 2023Quote: ... Ovaries were washed 4×20min in PBT and then incubated in PBT containing DAPI (1:1000, Molecular Probes), with or without DiOC6 (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: ... the viral medium was replaced with induction medium composed of DMEM/199 (4:1) (Gibco, Thermo Fisher Scientific) containing 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the viral medium was replaced with induction medium composed of DMEM/199 (4:1) (Gibco, Thermo Fisher Scientific) containing 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... to form embryoid bodies (EBS) in mTeSR1 supplemented with 50 ng ml−1 BMP-4 (Life Technologies, PHC9531), 50 ng ml−1 VEGF (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted 1:4 and 2 μL was used in a 20 μL qPCR Taqman assay (Invitrogen). Assay probes were used to detect cDNA from Nkx2.9 (Mm00435145_m1) ...
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bioRxiv - Biochemistry 2024Quote: ... GMPCPP-stabilized microtubule seeds were prepared by polymerizing a 1:4 molar ratio of tetramethylrhodamine (TAMRA, ThermoFisher Scientific) labeled:unlabeled tubulin (Hyman et al. ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 4 ℃) with primary IgG antibodies: rat anti-Ki67 (14-5698-80, SolA15, 1:400, Invitrogen, Carlsbad, CA, USA), rat anti-BrdU (ab6326 ...
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bioRxiv - Microbiology 2024Quote: ... the grid was blotted for 4 sec with a force of 1 using a Vitrobot Mark IV (ThermoFisher) and plunge-frozen into liquid ethane ...
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bioRxiv - Neuroscience 2024Quote: iPSC neurons were loaded with calcium-sensitive fluorescent Fluo-4 dye (1×, Direct Calcium Assay Kit, Invitrogen, USA) in humidified incubator (5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were then incubated overnight at 4°C with a P2RY12 primary antibody (1:100, 702516, Thermo Fisher) or TMEM119 primary antibody (1:50 ...
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bioRxiv - Synthetic Biology 2022Quote: HEK:BFP cells were the kind gift of Chris Richardson.8 Cells were maintained at 37°C and 5% CO2 in DMEM media plus GlutaMax (ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Genetics 2019Quote: Xenograft mouse model of human lung cancer tumors were implanted under the left upper limb in the abdomens of 6- to 8-week old male NCG mice by subcutaneous injection of A549 (5×106 cells in 200 µL DPBS (Gibco, C14190500BT)) or H2228 cells (2× 106 cells in 200 µL DPBS) ...
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bioRxiv - Microbiology 2021Quote: ... ExpiCHO cells were cultured in a shaker incubator at 33 °C at 115 rpm with 8% CO2 according to the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13 to 14 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... and 2×105 cells were seeded into 24-well culture plates or 5×104 cells into Lab-Tek II 8-well chamber slides (Nunc/Thermo Fisher). To account for donor variations ...
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bioRxiv - Immunology 2021Quote: ... ExpiCHO cells were cultured in a shaker incubator at 33 °C at 115 rpm with 8% CO2 according to the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13-14 days after transfection ...
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bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were maintained at 37°C with 5% CO2 with daily replacement of Essential 8 media system (Thermofisher, Canada. Catalog#A1517001). Cells were passaged as aggregates every 3-4 days until they reach 60-70% confluency with 0.5mM EDTA diluted in PBS (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Human nasal fluid samples were sonicated on ice (5 seconds at amplitude of 8 um; Fisher Scientific Model 705 Sonic Dismembrator) to create homogenous samples ...
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bioRxiv - Developmental Biology 2021Quote: ... H9 cells were cultured at 37°C and 5% CO2 in feeder-free conditions in Essential 8™ (E8 medium (Gibco, Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... heated at 95 °C for 5 min and were run in on 8–16% NuPAGE SDS-PAGE gradient gel (Life Technologies) in MOPS SDS Running Buffer (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The second half of the data (runs 5-8) was obtained after peptide separation on 75 μm columns packed with 2.6 μm Accucore beads (Thermo Fisher Scientific). Peptides were injected onto 30-40 cm ...
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bioRxiv - Neuroscience 2022Quote: ... They were cultured in 37C with 5% CO2 on geltrex-coated tissue culture plates in Essential 8 complete medium (E8; Gibco, #A1517001) with daily media changes ...
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bioRxiv - Developmental Biology 2023Quote: Single-cell isolated from day 8 organoids (200 unselected organoids) were incubated for 5 minutes at room temperature with Calcein-AM (C3099, Life Technologies) and subsequently sorted on a FACS Aria (BD ...
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bioRxiv - Immunology 2023Quote: Purified CD11c+ myeloid cells (5 × 104 cells) from bone marrow cultures were plated on an 8 well glass chamber slide (Nunc Lab-Tek-Thermo Fisher) in supplemented IMDM medium and incubated for 24 h ...
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bioRxiv - Physiology 2024Quote: Total mRNA was extracted from 5 biological replicates of 8 females (once-mated, aged 9 d) for each genotype using Trizol reagent (Invitrogen 15596018) and Direct-zol RNA Miniprep Plus Kits (R2072) ...
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bioRxiv - Physiology 2022Quote: ... 125 mL of medium was centrifuged at 8000 rpm (9730 x g) for 15 min (Sorvall RC 6 Plus; Thermo Scientific, Waltham, MA, USA). The supernatant was discarded ...
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bioRxiv - Cell Biology 2020Quote: ... Cells recovered from the first 300 × g pellet were pooled with cells detached from the culture plates by incubation at 4 °C in trypsin-EDTA (Gibco) as adherent cells and counted by Countess (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 was isolated from 0.35 mg of lysate by 90 min incubation at 4°C with 20 µl Protein-G Dynabeads (ThermoFisher, 10004D) and 2 µg of each of three anti-CDC20 mAbs ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 13,000 x g for 15 min at 4 °C and RNAs were lightly digested by treatment with MNase (Thermo Scientific) at a final concentration of 0.05 U μl-1 for 20 min at 16 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... and centrifuged for 60 min at 133,000 x g at 4°C in a Sure Spin 630 36 mL rotor (Thermo Scientific). The media was discarded and the crude pellet was resuspended in a minimal volume of PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... and centrifuged at 13,000 x g for 15 min at 4 °C and RNAs were lightly digested by treatment with MNase (Thermo Scientific) at a final concentration of 0.05 U μl-1 for 20 min at 16 °C ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were subjected to high speed centrifugation at 16,000 x g for 2 hours at 4°C (Sorvall LYNX 6000, Thermo Scientific). The virus pellet was suspended in 0.9% NaCl and sonicated on ice at 20kHz frequency and 70% amplitude for 15 seconds x 3 cycles (FB505 ...