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Citations for Thermo Fisher :
6351 - 6400 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... qPCR was performed on a QuantStudio™5 Real-Time PCR System (Thermo Fisher Scientific) using standard Taqman Relative Quantification protocol.
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bioRxiv - Microbiology 2022Quote: ... 4-5 ×106 P25 TCR-Tg CD4+ T cells (CD45.1) were labeled with CellTrace (Invitrogen) and adoptively transferred by tail vein injection in 100 µL of sterile PBS 24 hours (h ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µl of detecting mouse Ub antibody (ThermoFisher, 14-6078-37, P4D1, 5 µg/ml) were added to each well and incubated again for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation at 37°C for 5 minutes in a Cytomat 6001 (Thermo Scientific). Coelenterazine 400a working solution (50 μM DBC and 1% Pluronic F-127 in Tyrode’s buffer ...
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bioRxiv - Plant Biology 2024Quote: ... was used and the reaction was performed on Quantstudio 1 and Quantstudio 5 (Applied Biosystems). Actin2 (AT3G18780 ...
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bioRxiv - Systems Biology 2024Quote: ... restriction enzyme then 5’ overhangs were filled in by dNTPs with Biotin-14-dCTP (Invitrogen) using DNA Polymerase I and Large (Klenow ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were passaged as aggregates every 5-7 days using a collagenase IV solution (Gibco) to detach hESC colonies ...
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bioRxiv - Neuroscience 2024Quote: ... 3-5 mm skin biopsies were collected in Biopsy Collection Medium (RPMI 1460 [Thermo Fisher] with 1X Antibiotic-Antimycotic [Thermo Fisher]) ...
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bioRxiv - Developmental Biology 2024Quote: ... After 5 days in culture EBs were harvested and TrypLETM Express Enzyme (1×) (Gibco, 12605036) was used to generate a single-cell suspension.
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bioRxiv - Neuroscience 2024Quote: ... and cells were split every 4–5 days using 0.5 mM EDTA (Invitrogen, 15575-020). hiPS cell clones/lines were also generated using retroviruses or non-integrating episomal vectors from fibroblasts (Bhaduri et al. ...
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bioRxiv - Molecular Biology 2024Quote: THP-1s were cultured at 37°C in 5% CO2 in RPMI 1640 media (Invitrogen) supplemented with 10% heat-inactivated FBS (Thermo-Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... macrophages were stained with CellTracker Green 5-chloromethylfluorescein diacetate (CMFDA) dye (Cat. C7025, Invitrogen™) and imaged every 2 min for 60 min using a Zeiss LSM700 confocal microscope ...
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bioRxiv - Microbiology 2023Quote: ... wells were stained for nuclei with 5 μg/ml Hoechst 33342 (Thermo Fisher Scientific, USA) and incubated for 10 mins at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... The qPCR reactions were run on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... the RNA carrier was replaced in RAV1 buffer by 5 μg of linear acrylamide (Ambion). Nucleic acids were eluted in 50 μl of RNase-free water and stored at −80°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Add pre-mix chewing solution (5 μl 10xNEBbuffer2.1, 3 μl 100 mM DTT (ThermoFisher, A39255), 2 μl 100 mM ATP (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged once every 5-7 days using 0.5 mM EDTA (Life Technologies, #AM9260G) in phosphate-buffered saline (PBS)-/- (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Cell lines were routinely passaged every 5 days by dissociation using Accutase (A1110501, Thermo Fisher) for 10 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... for human β-globulin the mastermix was made using 5 μL Fast SYBR Green (ThermoFisher), 1 μL of hbg1 primer (3 μM) ...
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bioRxiv - Neuroscience 2024Quote: ... 5% CO2 in a humidified incubator using complete Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were maintained at 37C 5% CO2 and grown in IMDM media (Gibco #11995-065)+10% Fetal Bovine Serum +5% penicillin-streptomycin (Gibco #1514022) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μl of cell lysate was added to 50 μl of Galacton-Plus (Thermo Fisher), mixed rotationally for one hour at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were incubated with 5 µL of Dynabeads MyOne Streptavidin C1 beads (Invitrogen, 65001) on a slow rotator at room temperature for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of PageRuler Unstained or PageRuler (Plus) Prestained Protein Ladder (Thermo Fisher Scientific, USA) and 20 µl of each cell lysate sample (approx ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR was performed with Scientific QuantStudio 5 and 7 Real-Time PCR System (Thermo Fisher) using the DyNAmo Color Flash SYBR Green Mix (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Acclaim PepMap 100 nanoViper C18 (Thermo Scientific, 100 μm×2 cm, 5 μm, 100 Å) was used as a trap column ...
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bioRxiv - Microbiology 2024Quote: ... homogenized and placed in sterile 5 mL Eppendorf tubes containing 3 mL of RNAlater (ThermoFisher). The tubes were stored at -20°C upon our arrival in the laboratory ...
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bioRxiv - Microbiology 2023Quote: ... Differences in gene expression were determined by Real-Time PCR (Quantstudio 5, Thermo Fisher Scientific). Raw cycle threshold (CT ...
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bioRxiv - Microbiology 2024Quote: ... The ligation products (5 μl) were transformed into DH10B Competent Cells (Thermo Scientific, Rockford, IL) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 mL Penicillin/Streptomycin (100 U/mL and 100 μg/mL, respectively, ThermoFisher 15140122) at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... MS-5 cells were seeded into 0.2% gelatin coated 96 well-flat bottom plates (Nunc) at a density of 4-5×105 cells per well in Myelocult medium (H5100 ...
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bioRxiv - Immunology 2024Quote: ... Neurons were diluted in 5 ml Neuron Growth Medium (Neurobasal media (Life Technologies, cat#A3582901) supplemented with 2% B27 (Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... isolated CD4+ T cells were labeled with 5 mM CellTrace Violet Cell proliferation (Life technologies) per the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged once every 5-7 days using 0.5 mM EDTA (Life Technologies, #AM9260G) in PBS-/- (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... humidified incubator at 37 °C with 5 % CO2 (Hera Cell 240, Thermo Scientific, Essex, UK). TSPO KO microglia were generated using GeneArt™ CRISPR Nuclease Kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... from 5-30% solvent B (LC-MS grade 0.1% formic acid (#A117, Thermo Fisher Scientific) and acetonitrile ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with a mix of 5 µM Fura-2 AM (Invitrogen™, F1221) and 0.025% Pluronic® F-127 (diluted in CM ...
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bioRxiv - Cell Biology 2023Quote: ... All antibodies were diluted in 5% BSA and 0.1% Tween-20 (cat#J20605AP, Fisher Scientific). The following antibodies/dilutions were used ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μM of CellROX™ Green (0.5 μM for INS-I cells; Thermo Fisher Scientific) was added for 45 min at 37°C ...
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bioRxiv - Genetics 2024Quote: ... and run using standard parameters in a QuantStudio 5 Real-Time PCR System (Applied Biosystems). Thermo Fisher cloud design and analysis software (DA2 ...
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bioRxiv - Microbiology 2024Quote: ... BHK BSR-T7/5 cells were maintained under selection with 1 mg/mL Geneticin (Gibco) supplemented in the growth media every other passage ...
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bioRxiv - Immunology 2024Quote: ... using: anti-Keratin-5 (Chicken anti-mouse; polyclonal; dilution 1:500 in blocking solution; Invitrogen); anti-Keratin-8 (Guinea Pig anti-mouse ...
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bioRxiv - Genomics 2024Quote: ... solution (1mg L-Cystine, Sigma; 8 KU of DNase I, Affymetrix; in 5 ml DPBS), retina was incubated at 37C for ∼20min ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer instructions and labeled with 5 M carboxyfluorescein succinimidyl ester (CFSE, Thermofisher Scientific) for 10 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM Hepes-K+ pH 7.6) containing 2 mg/mL of collagenase type II (GIBCO) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted 5s with blot force 5 after 60s incubation in a Vitrobot IV (Thermo Fisher) at 6 °C using 100% humidity and flash-frozen in liquid ethane.
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed with ACK Lysing Buffer (Gibco, 3 ml for 5 min). The cells were counted and resuspended in RPMI-1640 medium (Corning ...
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bioRxiv - Bioengineering 2024Quote: ... These standards were incubated on a QuantStudioTM 5 Real-Time PCR thermocycler (Applied Biosystems #A47327) at an annealing temperature of 65°C with fluorescence measured every 60 seconds for 45 minutes (min) ...