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Citations for Thermo Fisher :
6551 - 6600 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 5 mM MgCl2) using ZebaTM Spin Desalting Column with a 7k MWCO (Thermo Fisher Scientific). A serial dilution of the protein samples was prepared in a black 384-well non-binding microplate (Greiner Bio-One ...
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bioRxiv - Biochemistry 2024Quote: ... followed by 30 minutes with streptavidin-PE (5 µg/mL, Thermo Scientific #12-4317-87). Results were read on a Luminex xMAP Intelliflex system.
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bioRxiv - Physiology 2024Quote: ... and donkey anti-rat DyLight 550 (5 µg/mL, Cat. # SA5-10027; Invitrogen, Darmstadt, Germany) for 1 hour followed by incubation with Hoechst (2 µmol/L ...
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bioRxiv - Physiology 2024Quote: ... donkey anti-mouse Alexa Fluor 647 (5 µg/mL, Cat. # A-31571; Invitrogen, Darmstadt, Germany), or donkey anti-rabbit Alexa Fluor 555 (6.67 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS cells were cultured at 37°C and 5% CO2 in McCoy’s 5A medium (Gibco) supplemented with 10% FBS and 100 U/ml penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... coverslips were incubated for 5 min at room temperature with Hoechst 33342 (Life Technologies H3570) diluted 1:1000 in double-distilled water to stain cell nuclei ...
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bioRxiv - Genetics 2024Quote: ... as internal size standard and results were processed by GeneMapper™ Software 5 (Applied Biosystems). The fragment sizes were converted to HTT CAG repeat numbers by comparison to predetermined HTT CAG repeat numbers of standard DNA (Standard Reference Material® 2393 ...
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bioRxiv - Cell Biology 2024Quote: ... L4 worms were sterilized by 5-fluorodeoxyuridine treatment (CAS number 50-91-9, Acros organics), and lifespan was measured by testing the worm movement with gentle poking using a worm pick ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated in 5× SSC-T buffer supplemented with 1 μg/mL DAPI (Invitrogen) for 20 min at room temperature with gentle rocking ...
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bioRxiv - Plant Biology 2024Quote: ... Cells were disrupted by passing them 3-5 times through a French press (Thermo Fisher) and centrifuged for 50,000g for one hour at 4°C ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was run on a QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific) using the Maxima SYBR Green/ROX qPCR Master Mix (2X ...
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bioRxiv - Cancer Biology 2024Quote: ... Treated cells were then counterstained with DAPI for 5 minutes and ActinGreen 488 (Thermofisher, R37110) for 20 minutes at RT ...
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bioRxiv - Systems Biology 2024Quote: ... each with largest dimension <5 mm and preserved in RNAlater (AM7021, Thermo Fisher Scientific, US) for RNA extraction ...
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bioRxiv - Bioengineering 2024Quote: ... adding 5 mM GMP and 4 U/µL RNaseOUTTM recombinant ribonuclease inhibitor (Thermo Fisher Scientific) to the transcription reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 cells in 50-100 μls DPBS (Ca2+/Mg2+ free, Thermo Fisher Scientific) were implanted into the #4 mammary fat pads of 21 days-old FVB/n female recipient mice (Jackson Laboratory ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked with SEA BLOCK buffer (Thermo Scientific; diluted 1:5 in PBS) for 1 hour at RT and incubated overnight at 4°C with primary antibodies (rabbit anti-GFP ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with 250 mL streptavidin-conjugated paramagnetic beads (5 mg/ml, Thermo Scientific, 88817) for 2 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Tol 5 whole genome database (AP024708, AP024709; NCBI) were performed using SEQUEST (Thermo Fisher Scientific). The search parameters were set as follows ...
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bioRxiv - Microbiology 2024Quote: ... and/or blood agar (BA, tryptic soy agar with 5% sheep blood, Thermo Fisher Scientific). Note that both PTA and BA plates contain heme precursors ...
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bioRxiv - Microbiology 2024Quote: ... parasites were rinsed in PBSTx then permeablized in 5 μg/ml proteinase K (Ambion AM2546). Following proteinase K treatment ...
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bioRxiv - Microbiology 2024Quote: ... 5’-Biotinylated probes were hybridized to the membrane overnight at 42°C in ULTRAhyb (Ambion) hybridization buffer ...
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bioRxiv - Microbiology 2024Quote: ... All qPCR assays were performed using the QuantStudio 5 real-time PCR system (Applied Biosystems). Cycling conditions were an initial two minute incubation step at 50°C ...
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bioRxiv - Neuroscience 2024Quote: ... then an IP injection of 50 mg/kg 5-ethynyl-2’-deoxyuridine (EdU (ThermoFisher Scientific)) 24 h later and were perfused after a further six days.
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bioRxiv - Plant Biology 2024Quote: ... 5′ ends of gene-specific cDNAs were amplified with Platinum® Pfx DNA polymerase (Invitrogen) and subsequently cloned into pCR®4-TOPO® for Sanger sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed on 1-5 μg of RNA using SuperScript IV (ThermoFisher 18090010) and 1 μL of 50 mM custom RT primer (Primer 4 ...
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bioRxiv - Neuroscience 2024Quote: ... We then performed an overnight coating with 5 µg/mL mouse Laminin (Fisher Scientific # CB40232) and 5 µg/mL human Fibronectin (Fisher Scientific # CB40008 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5 µl of transfection solution: 0.1 µL Lipofectamine™ 2000 (cat. #: 11668500, Life Technologies) diluted to 5 µl total volume with Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... the mixture was centrifuged at 16,000 ’g for 5 min at -10 °C (Thermo Scientific™ Sorvall™ Legend™ XTR Centrifuge ...
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bioRxiv - Microbiology 2024Quote: ... 20 mM glucose) before being loaded with 5 µM Fluo-4 AM (Invitrogen, Waltham, MA) with 1% PowerLoad Concentrate ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl RNA (∼ 2.5 μg) was reverse transcribed into cDNA (20 μl reaction, SuperScriptIII, Invitrogen) using random hexamers and amplified by RT-qPCR in a LightCycler 480 System (Roche Applied Science) ...
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bioRxiv - Pathology 2024Quote: ... 5 µg of plasmid mix was incubated with Lipofectamine 3000 transfection reagent (Thermo Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cascade blue Dextran (5 % w/v, 10,000 kDamw, 100 μl, D-1976, Life Technologies, USA) was injected intravenously 10 minutes before imaging and excited at 820 nm to visualize the vasculature ...
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bioRxiv - Microbiology 2024Quote: MRC-5 fibroblasts (ATCC) were propagated in Dulbecco’s modified Eagle medium (DMEM) (Thermo Fisher Scientific) containing 7% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The helper virus was diluted to 5 × 1010 vg/ml in sterile DPBS (Gibco, ThermoFisher) before use for a specific Cre-dependent TVA expression as recommended by a previous paper (75).
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bioRxiv - Neuroscience 2024Quote: ... The helper virus was diluted to 5 × 1010 vg/ml in sterile DPBS (Gibco, ThermoFisher) before use for a specific Cre-dependent TVA expression as recommended by a previous paper (75).
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bioRxiv - Cell Biology 2024Quote: ... and 5 g of lipid-rich bovine serum albumin (Albu-MAX II, Thermo Fisher Scientific) for 1 L of sterile water (pH 7.4) ...
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bioRxiv - Microbiology 2024Quote: ... 100 to 300 nM of each siRNA and 5 uL of Lipofectamine (Invitrogen, 11,668-019) were separately incubated in Opti-MEM for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The PMN pellet was resuspended in 20 mLs ACK lysing buffer for 5 minutes (Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... for 5 minutes at 4°C before quenching in 1x Phosphate Buffered Saline (PBS; Gibco). Cell viability was quantified by staining with Acridine Orange/Propidium Iodide at a 1:1 dilution in a Cellometer slide before reading on an Auto2000 Cellometer (Nexcelom Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... somatosensory cortices from 5 embryos were microdissected in ice-cold HBSS 1x (Gibco, cat#14025092). For postnatal timepoints ...
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bioRxiv - Cell Biology 2024Quote: ... 40 µl lysates were boiled for 5 min in Bolt LDS sample buffer (ThermoFisher B0008) before running on 12% bis-tris gels at 165 V ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were kept at 37°C and 5% CO2 in neurobasal medium (Thermo Fisher Scientific) containing 2 mM L-glutamine (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Each iWAT and eWAT depot received 4–5 μL of 0.1% CTB-488 (Invitrogen C34775) or CTB-647 (Invitrogen C34778 ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS-T (0.5% Triton-X100, 9036-19-5 Sigma Aldrich, in PBS, 10010023 Gibco). For overnight staining at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... supplemented with 10% FBS (GenClone) and 5 µg/mL of Penicillin/Streptomycin (Thermo Fisher Scientific) and grown in ventilated flasks at 37 °C in a 5% CO2 incubator prior to experiments ...
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bioRxiv - Systems Biology 2024Quote: ... siRNA was diluted to 5 μM solution in DNase/RNase free water (Invitrogen, 10977-035). In separate tubes ...
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bioRxiv - Microbiology 2024Quote: ... at 5×103 cells/well in 100μL 10% FBS Phenol red-free RPMI (ThermoFisher Scientific) and incubated ∼18-24 hours at 37°C 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membrane was incubated with 5 mL with either SuperSignal West Pico PLUS Substrate (ThermoFisher Scientific) or SuperSignal West Femto Substrate (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... approximately 2-5×106 cells were incubated in 40 μl of fixable viability dye (Invitrogen) diluted in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 μl Cy3-UTP (5 mM) and 0.4 μl RiboLock RNase Inhibitor (Thermo Fisher Scientific) and was incubated for 2 hours at 37°C ...