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Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Mouse lung tissue sections were sectioned via cryostat (Thermofisher CryoStar NX70 cryostat, 5 μm/section) and fixed onto glass slides ...
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bioRxiv - Microbiology 2021Quote: ... with 5% HyClone iron-supplemented calf serum (Cytivia, SH30073.03) and 1% penicillin-streptomycin (Invitrogen, 15140122), and were maintained at 37 °C and 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... then fixed with 4% paraformaldehyde containing 5 µg/ml Hoechst 33342 stain (Thermo Fisher, #62249) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 150nM of each primer and 5 μl 2X-Jump Start SYBR Green PCR Mix (Invitrogen) in10 μl total volume ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... An anion-exchange HPLC column (BioBasic AX, 150 x 2.1 mm, 5 µM, Thermo Scientific) was used for the chromatographic separation and a 5500 QTrap (Sciex ...
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bioRxiv - Immunology 2021Quote: C-LP cells pooled from 5-7 mice were sorted into TriZol® (Thermo Fisher) and cDNA was generated by using QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... For experiments using OP9 stromal cells: OP9 cells were labeled with 5 μM CFSE (Invitrogen). Immune complexes containing mono-biotinylated lysozyme and anti-sera generated in mice were formed in vitro ...
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bioRxiv - Systems Biology 2020Quote: ... then re-suspended in 40 μl of 5% mass spectrometry grade acetonitrile (Thermo Fisher Scientific) and 0.5% formic acid ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.05 μl of Platinum Taq DNA Polymerase (5 U/μl, Invitrogen, Carlsbad, CA, USA). Gene expression was evaluated using a modified 2-ΔCT method (Schmittgen and Livak 2008) ...
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bioRxiv - Cancer Biology 2020Quote: ... at 37°Cin a humidified incubator with an atmosphere of 5% CO2 (Thermo fisher scientific). The cells were passaged at a density of 2.0×105 cells in a 25cm2 flask or cryopreserved at a density of 2.0×106/mL.
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bioRxiv - Genomics 2021Quote: ... The diluted (1/5) total cDNA was subjected to Taqman gene expression (ThermoFisher Scientific, USA) using the following transcript-specific probes and primers ...
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bioRxiv - Genetics 2021Quote: ... 3×105 U2OS cells were incubated with 5 pmol siRNA using lipofectamine RNAiMAX (Thermo Fisher) in a 12-well tissue culture plate for 2hr ...
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bioRxiv - Cell Biology 2021Quote: ... the caudal disc sections were blocked in 5% normal serum (Thermo Fisher Scientific, 10000 C) in PBS-T (0.4% Triton X-100 in phosphate-buffered saline (PBS)) ...
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bioRxiv - Genetics 2020Quote: ... and 5% (v/v) heat-inactivated fetal bovine serum (FBS) (all supplements were from Invitrogen). Transient transfection was developed with PEI method and BRET assays were performed as previously described (Reyes-Resina et al. ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 5 µM of the membrane dye FM4-64 (Invitrogen, cat# LST3166) for 5 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 5’-RACE procedure was performed using the FirstChoice® RLM-RACE Kit (Invitrogen™). cDNA containing the 5’UTR of CD44 from each cell type was amplified using a nested PCR strategy with the primers:
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bioRxiv - Cell Biology 2020Quote: ... 5% CO2 and atmospheric O2 in a humidified incubator (Thermo Fisher Scientific, Waltham, MA, USA). A549 cells were purchased from Beina Biology (Shanghai ...
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bioRxiv - Cell Biology 2020Quote: ... dried and mounted on microscopy glass slides with Prolong Diamond antifade 5 (Thermo Fisher Scientific). Slides were cured overnight at room temperature and imaged the next day on a spinning disk Eclipse Ti2-E inverted microscope (Nikon) ...
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bioRxiv - Biochemistry 2020Quote: ... packed with 5 μm particles with a pore size of 100 Å (Thermo Fisher Scientific) and separated on an analytical C18 100 column of dimensions 500 mm x 75 μm i.d. ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 μg of purified plasmid DNA was mixed with 5 μl Lipofectamine 3000 (Thermo Fisher) and used for transfection according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Cells were resuspended 48 h post-infection and fixed in 5% formaldehyde in PBS (Gibco) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 23°C ...
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bioRxiv - Biochemistry 2021Quote: ... a PepSwift trap is used pre-RP (200 μm x 5 mm, Thermo Scientific DX164558). Samples are eluted from the former with an injection of 10 μL acetonitrile and from the latter by an RP gradient ...
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bioRxiv - Neuroscience 2020Quote: ... and split 1:5 on T25 flasks (Thermo-Fisher Scientific; Cat. No. 12-556-009) coated with poly-L-ornithine (PLO ...
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bioRxiv - Neuroscience 2021Quote: ... incubated for 5 minutes with nuclear counterstain Hoechst 33342 (1:1000 in PBS, ThermoFisher #H3570), rinsed 3 x 5 minutes in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were eluted from beads by boiling for 5 min in LDS sample buffer (ThermoFisher) and the eluates analyzed by Western blot as described above.
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bioRxiv - Synthetic Biology 2021Quote: ... the samples were incubated at 56 °C for 1 hour with 5 mM dithiothreitol (ThermoFisher). Then ...
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bioRxiv - Biophysics 2021Quote: ... K562 cells were cultured at 37 °C with 5% CO2 in DMEM with GlutaMAX (GIBCO) supplemented with 10% FBS and 1% penicillin-streptomycin for two days ...
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bioRxiv - Microbiology 2020Quote: ... Cells were grown at 37 °C and 5% CO2 in DMEM (Gibco, cat. no. 11965) with high glucose (4.5 g l-1 ...
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bioRxiv - Biochemistry 2021Quote: ... at 37°C in RPMI 1640 medium supplemented with 5 g/L AlbuMAX II (Gibco), 2 g/L NaHCO3 (Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were maintained at 5% CO2 at 37°C in DMEM with glutamine (Invitrogen) supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Cell Biology 2021Quote: 5×103 C2C12 cells were cultured on a Nunc™ glass base dish (Thermo Scientific) coated with fibronectin and cultured overnight ...
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bioRxiv - Cell Biology 2021Quote: For expression of proteins 5 ml of an overnight culture of BL21 DE3 AI (Invitrogen) E ...
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bioRxiv - Genomics 2020Quote: ... and 30s at 60 °C in QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific). The sequences of the probes and primers are listed in supplementary table 2 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight (4° C) with 5 µg of Cx36 (Thermofisher, 37-4600, RRID: AB_2533320) or NMDAR1 (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... 5-10 ul of each sample was loaded on 4-12% Bis-Tris gels (Thermofisher).
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bioRxiv - Immunology 2021Quote: ... or L9.6 T cells were stained with 1-5 μM of CTV or CFSE (Invitrogen) according to the manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1 μL Platinum Taq DNA Polymerase High Fidelity (5 U/μL) (Invitrogen, Carlsbad, CA, USA), and 1.0 μL template cDNA ...
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bioRxiv - Immunology 2021Quote: ... THP-1 monocytes were cultured during 5 days in RPMI-1640 Medium (Gibco, MD, USA); 2-mercaptoethanol (0.05 mM final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... on 35 mm dishes (37 °C, 5 % CO2) coated with Geltrex□ (Gibco, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... on 35 mm dishes (37 °C, 5 % CO2) coated with Geltrex□ (Gibco, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside in DMSO (0.1-1mM, AICAR, Fisher Scientific), or isoproterenol (5-25µM ...
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bioRxiv - Molecular Biology 2021Quote: ... Differentiated organoids were broken using a 5-minute incubation with TrypLE (TrypLE Express; Life Technologies). After shearing ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% Triton X-100 and 5 mM dithiothreitol (DTT) supplemented with Ribolock (Thermo Fisher Scientific) and proteinase inhibitor cocktail (Roche) ...
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bioRxiv - Immunology 2020Quote: ... VERO cells were overlaid with 0.8% agarose in DMEM medium containing 5% FBS (Invitrogen, CA) and incubated at 37°C for three days ...
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bioRxiv - Immunology 2021Quote: ... Prepare for biotin pull-down by washing 5 μl of Streptavidin C-1 (Invitrogen, 65001) beads with Tween Wash Buffer (5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC; Thermo Fisher Scientific), respectively ...
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bioRxiv - Immunology 2020Quote: ... After this 1⨯10 5 BMDC per condition were cultured on a collagen (Fisher Scientific) coated6-well plate (Corning ...
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bioRxiv - Immunology 2020Quote: ... macrophages and synovial fibroblasts were labelled with CellTrace™-Far Red (5 μM, Life Technologies) and CellTrace™-Violet (5 μM ...
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bioRxiv - Microbiology 2020Quote: ... diluted in water 70:30 (Optima LC/MS Grade Water, 7732-18-5, Fisher Scientific) was added ...