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Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: NSP1 5’ end fragments were amplified by Phusion Hot Start II DNA Polymerase (Thermo Scientific) following the manufacturer’s guide ...
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bioRxiv - Biochemistry 2021Quote: Outgrown cells were plated dropwise on Nunc OmniTrays (5 μL per plate) (Thermo Fisher; 140156), containing LB-agar + carbenicillin at 100 µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and 5% deep red fluorescent carboxylate-modified beads (0.2 μm diameter; F8810; Thermo Fisher Scientific). 13 μL of the mixture was added to a 35 mm glass-base dish (IWAKI ...
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bioRxiv - Biophysics 2020Quote: Biotinylated 5 kb long DNA was synthesized by PCR using ΦX174 RF1 DNA (ThermoFisher Scientific) as a template and oligonucleotides 5’ biotin-CGAAGTGGACTGCTGGCGG-3’ and 5’-CGTAAACAAGCAGTAGTAATTCCTGCTTTATCAAG-3’as primers ...
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bioRxiv - Biophysics 2020Quote: ... These two oligonucleotides were phosphorylated at the 5’-end using Polynucleotide Kinase (PNK, ThermoFisher Scientific) reaction (1 μM of the respective oligonucleotide ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg RNA was reverse transcribed to cDNA using Superscript III Reverse Transcriptase from Invitrogen. cDNA was diluted 1:100 and real-time PCR was performed in triplicate with iQ SYBR Green Supermix from BioRad.
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bioRxiv - Bioengineering 2021Quote: ... 6-well well-plates were first coated with vitronectin (5 μg/mL, Thermo Fisher, USA) followed by a coating with 10% fetal bovine serum (ThermoFisher ...
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bioRxiv - Bioengineering 2020Quote: ... and Alexa Fluor®647-labelled transferrin (Thermo Fisher Scientific T23366; 5 mg/mL stock) in the same run buffer ...
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bioRxiv - Bioengineering 2020Quote: ... ITS+1 (10 µg/mL insulin, 5.5 µg/mL transferrin, 5 ng/mL selenium; Gibco), 100 µM ascorbic acid 2-phosphate (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... 5% (v/v) glycerol) supplemented with a protease inhibitor cocktail (EDTA-free, Thermo Scientific Pierce) and mechanically lysed using a homogeniser ...
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bioRxiv - Biochemistry 2021Quote: ... DRGs were dissected from the embryos at Day 12.5 to 14.5 (E12.5-E14.5) and digested by 0.05% Trypsin solution containing 0.02% EDTA (Gibco). The dispersed cells were seeded in Neurobasal™ Plus Medium supplemented with 2% B27 plus ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were sampled on a 300 μm x 5 mm PepMap C18 precolumn (Thermo Scientific) and separated on 75 μm x 150 mm and 75 μm x 250 mm C18 columns (Gemini C18 ...
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bioRxiv - Neuroscience 2020Quote: ... Thermal cycling was performed on QuantStudio 5 real-time PCR System (Thermo Fisher Scientific Inc.) using a relative calibration curve ...
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bioRxiv - Immunology 2020Quote: ... isolated B cells were stained with Cell Proliferation Dye eFluor™ 670 (5 μM, Invitrogen) and measured after 48 and 96 h.
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bioRxiv - Immunology 2020Quote: ... Transgenic lymph node cells were labelled with 5-carboxyflurorescein diacetate succinimidyl ester (CFSE) (Molecular Probes) as described (14 ...
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bioRxiv - Neuroscience 2021Quote: ... The final buffer for the virus was: dPBS + 5% Glycerol + 0.001 Pluronic F-68 (Invitrogen). The AAV titre (1.71 × 1013 GC/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged 1:5 at ∼80-90% confluency with 0.25% Trypsin (15090-046; Gibco). Co-culture assays were performed by incubating cells in 2.5 μM CFSE (65-0850-84 ...
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bioRxiv - Biophysics 2020Quote: ... the medium was placed in fresh growth media with 5 μ g/mL blasticidin (Invitrogen) for 3 days to select for infected cells ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... CDH11 #4: 5’-CCUUAUGACUCCAUUCAAA-3’ using Lipofectamine RNAiMAX transfection reagent (13778030; ThermoFisher Scientific, Waltham, MA) in serum-free DMEM ...
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bioRxiv - Cancer Biology 2020Quote: Pellets from 5 × 106 cells were collected and digested by 500 μl RIPA Buffer (Invitrogen). Samples were incubated on ice for at least 15 minutes and centrifuged at 12,000 rpm for 10 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl of 5 mg/ml thiazolyl blue tetrazolium bromide (MTT) reagent (Thermo Fisher #M6494) in PBS was added per well and incubated at 37°C for 3 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged 1:5 at ~80-90% confluency with 0.25% Trypsin (15090-046; Gibco). Cells were routinely screened for mycoplasma ...
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bioRxiv - Neuroscience 2020Quote: ... claudin-5 (1 μg/ml) and occludin (2 μg/ml; all from Thermo Fisher Scientific). After incubation with primary antibodies membrane was washed with PBS-T three times on a platform rocker and incubated with horseradish peroxidase-conjugated secondary antibody diluted in PBS-T 1:2000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... for 5 h in Opti-MEM I Reduced Serum Medium without antibiotics (Thermo Fisher Scientific). MGE-derived neurons were transfected after 6 DIV ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5% CO2 in humidified incubators and passaged by digestion with 0.05% trypsin-EDTA (Thermo Fisher). Cell counting was performed using a TC20 automated cell counter (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... Trimer was reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, Thermo Scientific) and alkylated with 10 mM 2-chloroacetamide (Sigma Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... and centrifuged twice at 200xg for 5 min each in advanced DMEM/F12 (Gibco; 12634010). The DMEM/F12 was aspirated off and the cells were resuspended in mesoderm induction media (consisting of DMEM/F12 with GlutaMax (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... 100,000 cells were added to each well with 5 µM MitoSOX™ Red (Invitrogen, M36008). Immediately after the addition of MitoSOX ...
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bioRxiv - Cell Biology 2021Quote: ... the beads were loaded onto a 5 ml disposable polypropylene gravity column (#29922, Thermo Scientific) and washed with at least 10 CV SP wash buffer (50 mM sodium phosphate pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: HEK293 and HeLa cells were cultured at 37°C with 5% CO2 in DMEM (Gibco) with 10% heat-inactivated FBS (BioInd) ...
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bioRxiv - Microbiology 2020Quote: ... and at 5 days post infection cells were fixed in 10% formalin solution (Fisher Scientific) for 1 hr ...
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bioRxiv - Plant Biology 2021Quote: ... the 5’LTR sequence of GP1 (AT2TE23670) was cloned into the donor vector pDONR207 (Invitrogen) using primers PHG121 and PHG124 ...
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bioRxiv - Neuroscience 2021Quote: ... and transcript levels were quantified by qPCR (QuantStudio 5 Real-Time PCR system, Applied Biosystems) using the ΔΔCt method and GAPDH as reference (40) ...
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bioRxiv - Plant Biology 2021Quote: ... pollen tubes were incubated for 5 min with 50 μM H2DCFDA (Molecular Probes, Invitrogen, C6827) at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... pollen tubes were incubated for 5 min with 50 μM H2DCFDA (Molecular Probes, Invitrogen, C6827) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... washed with PBS and detached by incubating for 5 min with accutase (Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... peptides were trapped using a 0.3×5 mm PepMap-100 C18 pre-column (Thermo Scientific) of 5 µm particle size and 100 Å pore size prior to separation on an analytical column (50 cm of length ...
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bioRxiv - Neuroscience 2020Quote: ... DRGs were rinsed 5 times in L-15 with Penicillin/Streptomycin (Life Technologies, 15140-122) and minced in growth media (DMEM Ham’s F-12 ...
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bioRxiv - Microbiology 2021Quote: ... 1:5 000 dilutions of anti-rabbit or anti-mouse HRP-conjugated antibodies (Thermo Scientific), and SuperSignal Femto West (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Digestion was halted by addition of Fetal Bovine Serum (FBS) (5%) (GIBCO-Thermo Fisher Scientific) with DNase I (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Digestion was halted by addition of Fetal Bovine Serum (FBS) (5%) (GIBCO-Thermo Fisher Scientific) with DNase I (Sigma ...
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bioRxiv - Immunology 2021Quote: ... an EdU (5-ethynyl-2′-deoxyuridine) proliferation assay was performed as recommended by Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... keratinocytes were plated at 5 million cells per 10cm dish in M154 keratinocyte media (ThermoFisher) containing .07 mM Ca2+ and human keratinocyte growth serum (HKGS ...
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bioRxiv - Physiology 2020Quote: ... The qPCR reactions were run on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized with Maxima™ H Master Mix 5 x (Thermo Fisher Scientific, #M1661), followed by qPCR according to the PowerUp™ SYBR Green technology (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... was centrifuged for 5 minutes at 8000 rpm (Heraeus Megafuge 1.0, Thermo Scientific, Waltham, US) to the bottom of a 2-ml Eppendorf tube to form a 10-μl pellet ...
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bioRxiv - Cancer Biology 2020Quote: ... 7mM NaOH) followed by an incubation for 5 hours with Dynabeads Protein G (Invitrogen, 10004D). Beads were then washed with Wash Buffer (20mM Tris pH=8 ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted with the respective media and containing fluorescence microbeads at 5 × 109 beads/mL (ThermoFisher). The Matrigel solution was allowed to coat the collagen for 10 minutes before pipetting a cell suspension into the channel ...
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bioRxiv - Cancer Biology 2020Quote: ... Transduced cells were selected by addition of 5-15 ug/mL blasticidin (Life Technologies, Inc) two days post-transduction ...
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bioRxiv - Cell Biology 2021Quote: ... mitochondrial vesicles from ∼5×108 cells were resuspended in 100 μL NativePAGE sample buffer (Invitrogen), lysed with 1.5% dodecylmaltoside (DDM ...