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Citations for Thermo Fisher :
6201 - 6250 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Total protein of 40 μg was prepared from each sample and quantified by the Pierce™ BCA Protein Assay Kit (ThermoFisher). We ran the protein in sodium dodecyl sulfate-polyacrylamide (SDS ...
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bioRxiv - Genomics 2020Quote: ... the protein concentration of lysates was first determined using the Microplate BCA protein assay kit – reducing agent compatible (Thermo Fisher Scientific), according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... The total protein concentration of each sample was determined using the Pierce(™) BCA Protein Assay Kit (Thermo Fisher Scientific Inc.) and the saliva sample was prepared with equal concentrations of proteins from each individual sample ...
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Genomics 2021Quote: Protein lysates derived from same plates used for metabolomics collection were assayed for total protein concentration with Pierce BCA Protein Assay Kit (ThermoFisher 23225) for normalizing raw metabolite values ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein amounts of the OM cell lysates were quantified with BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...
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bioRxiv - Molecular Biology 2022Quote: LC-MS files were searched against Saccharomyces cerevisiae Swiss-Prot eIF4A protein sequence (Uniprot Protein Accession Number P10081) using Sequest HT embedded in Proteome Discoverer 1.4 (Thermo Fisher Scientific). Searching parameters include ...
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bioRxiv - Developmental Biology 2022Quote: ... and then normalized to both the internal standard (d5-2HG) peak area and the protein content of duplicate samples as determined by a BCA protein assay (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... Protein was extracted immediately after as described above and protein concentration was calculated following the PierceTM BCA Protein Assay Kit (ThermoFisher Scientific). A total of 600 µg were immediately used for the assay following manufacturer’s indications ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were transferred to new tubes and protein concentrations were determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). Between 20-50 μg of protein per sample was mixed with Laemmli sample buffer (62.5 mM Tris-HCl (pH 6.8) ...
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bioRxiv - Cancer Biology 2022Quote: ... 15-20 μg protein samples were run in SDS–PAGE followed by protein transfer using Mini Gel Tank and Mini Blot Module (Life Technologies). Immunoblotting was detected using near-infrared fluorescence (LI-COR ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein concentration of the cell lysates was then determined using Pierce™ BCA Protein Assay Kit from ThermoFisher (#23225). After 100 mM DTT was added ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was transferred into a new tube and the protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). Protein concentration was adjusted to 0.5 µg/µL using lysis buffer and 45 µL were stored at −80°C (“input”) ...
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bioRxiv - Neuroscience 2022Quote: ... tissue debris was removed by centrifugation and protein concentrations were determined by Pierce BCA Protein Assay Kit (ThermoFisher Scientific, catalog # 23225). 10 μg of proteins per sample were resolved by SDS-PAGE and transferred onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and measured for a protein concentration using the BCA™ protein assay (Thermo Fisher Scientific, MA, USA). The protein sample was diluted with Laemmli buffer containing β-mercaptoethanol (Bio-rad ...
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bioRxiv - Neuroscience 2022Quote: Approximately 10 μg of synaptosome protein was separated on a precast gradient gel (NuPAGE 4-12% Bis-Tris Protein Gels, NP0336BOX, Thermo Fisher) and transferred to nitrocellulose membrane (AmershamTM Protran® Western Blotting Membrane ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... 2 mg of total protein was incubated with the DNA-PKcs monoclonal antibody (25–4) and Protein A/G beads (Thermo Fisher) overnight at 4° C with mixing ...
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bioRxiv - Microbiology 2022Quote: Cell lysates from the ΔhipBST cultures (vector induced, HipTLp uninduced, and HipTLp induced) were quantified for total protein (Pierce BCA Protein Assay Kit, Thermo Scientific), and 2.5 mg of total protein from each sample was used ...
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bioRxiv - Physiology 2023Quote: ... 8 mg of the soluble protein fractions were pre-cleared with 200 μL of protein G-magnetic Dynabeads (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... 25-30 µg protein samples were run in SDS–PAGE followed by protein transfer using Mini Gel Tank and Mini Blot Module (Life Technologies). Immunoblotting was detected using near-infrared fluorescence (LI-COR ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining sample was denatured at 95°C for 5 minutes in 4× Laemmli buffer and subjected to SDS-PAGE using 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels followed by protein transfer to a 0.45-μm pore PVDF membrane (Cat#: 88585, ThermoFisher). Membranes were blocked in 3% BSA in TBS with 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Neuroscience 2022Quote: ... and protein concentration for each sample was determined using a BCA assay (Pierce Microplate BCA Protein Assay Kit, ThermoFisher, Waltham, MA). Equal amounts of protein were prepared using 4x NuPage sample buffer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet samples were analyzed for protein expression using SDS-PAGE and Western blotting coupled with a prestained protein marker-180kDa (Thermo-Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed with PBS before lysing with 350 ul RLT for total protein quantification using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... Final pellets were resuspended in HNE buffer and protein concentrations were measured using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to the manual provided by the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the lysates was determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher, Cat. no. 23225). Western Blots ...
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bioRxiv - Cell Biology 2022Quote: ... 2019).The quality of co-expressed proteins were evaluated through resolving the protein bands on SDS-PAGE gel (Bolt 4-12%, Invitrogen™) Following the completion of protein expression ...
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bioRxiv - Molecular Biology 2024Quote: ... Efficacy of the process and final protein purity was assessed by SDS-PAGE using NuPAGE 4-12 % Bis-Tris Midi Protein Gels (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... The molecular migration position of transferred proteins was compared with the PageRuler Prestain Protein Ladder 10– 180 kDa (Themo Fisher Scientific). Signal quantification was performed using Fiji ...
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bioRxiv - Neuroscience 2024Quote: PVN tissue biopsies were collected from fresh-frozen brain tissue and protein extracted using Neuronal Protein Extraction Reagent (N-PERTM, Thermo Scientific) for determination of CRH protein levels by Western Blot ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the CSF or plasma SEC fractions 7-10 was then determined using Qubit Protein Assay kit (ThermoFisher Scientific) using 1 μL of sample ...
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bioRxiv - Genomics 2024Quote: ... Protein quantification for each fraction was done using the manufacturer’s protocol for Pierce 660 nm protein assay reagent (Thermo Scientific, USA). Each Fraction was subjected to iCLIP protocol as described in Huppertz et al ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest of the sample was divided into equal amounts of protein (1.5 mg of protein/pull-down) and incubated with 80 μl of pierce streptavidin magnetic beads (Thermo Fisher Scientific), O/N ...
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bioRxiv - Molecular Biology 2024Quote: Antibody-bound complexes were then captured by incubation with Protein A sepharose CL-4B beads (Amersham, 17-0963-03) for rabbit antibodies or Protein G sepharose 4B beads (Invitrogen, 101241) for mouse antibodies (both 20 μL per 1 mL lysate ...
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bioRxiv - Cell Biology 2024Quote: SK-Mel28 cells were grown on coverslips and transiently transfected with ribonucleprotein complexes consisting of purified recombinant Cas9 protein (Truecut Cas9 Protein v2, Invitrogen, #36499) and synthetic guide RNAs (Invitrogen TrueGuide Synthetic sgRNA #35514 or Negative Control ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration of each lysate was determined by the Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Cell lysates (20 μg ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations in the supernatants were measured using the EZQ® Protein Quantitation Kit (Molecular Probes, Inc. Eugene, Oregon, USA) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 20,000 x g for 10 min at 4 °C and protein concentrations were determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 13,000 rpm for 10 min at 4°C and the protein concentrations were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein were separated on 8% SDS-PAGE and then transferred onto nitrocellulose membranes (Thermo Fisher) ...
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bioRxiv - Bioengineering 2023Quote: ... Values were normalized for total protein content in each well using a Pierce BCA Protein Assay Kit (Thermo Fisher, cat# 23225) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... Twenty μL of the crude extract was removed for protein quantification by a Bradford protein assay according to manufacturer’s instructions (Thermo Scientific Inc.). Next ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured by the bicinchoninic acid (BCA) assay using the Pierce BCA protein assay kit (ThermoFisher Scientific: 23225). Equal amounts of protein were loaded on 4 to 12% SDS-PAGE gel and transferred onto nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein contents of total cell lysates were determined using a BCA protein assay kit (Thermo Scientific Pierce, Rockford, IL, USA). The equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Molecular Biology 2022Quote: Antibodies targeting proteins of interest or IgG isotype control (5ug) were incubated with 20ul of Dynabeads Protein G (Thermo Fisher Scientific) in buffer WB150 (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Equal protein loads were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and proteins were transferred to nitrocellulose membranes (Thermo Scientific). For protein detection the following primary antibodies were used at the indicated dilutions ...