Labshake search
Citations for Thermo Fisher :
6101 - 6150 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Samples were homogenised using a VDI 12 tissue homogeniser and protein was quantified using a bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific). 100 μg of total lung protein were lysed in 2x sample buffer (4% [w/v] SDS ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
-
bioRxiv - Systems Biology 2021Quote: ... Lysates were cleared by centrifugation at 16,000 x g for 10 minutes at 4°C and protein concentration was determined using the BCA protein assay kit (Thermo Scientific). Fifty micrograms of protein was reduced with 5 mM Tris 2-carboxyethylphosphine (TCEP ...
-
bioRxiv - Microbiology 2021Quote: ... A portion (5 µl) was kept to quantify the total amount of proteins using Pierce™ BCA protein assay kit (23225, ThermoFisher). 5 ml of HNO3 5% (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... 10uL of the protein lysate was loaded into a bis-tris protein gel (4-12% Invitrogen Bolt Bis-Tris mini gel), ran at 150V for 35min then transferred to PVDF membrane using the iBlot2 system ...
-
bioRxiv - Microbiology 2021Quote: ... Eluted protein was buffer exchanged with the same elution buffer but without imidazole using 30 kDa MWCO protein concentrator (ThermoFisher Scientific). Final protein concentration was measured by spectrophotometry.
-
bioRxiv - Microbiology 2020Quote: ... as measured on protein level (BCA protein concentration determination kit; before digestion) was labeled with 10-plexing tandem mass tags (TMT-10; Thermo Scientific) following the manufactures instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Enriched virus was inactivated with 2% sodium dodecyl sulfate (SDS) and protein concentration determined with a Pierce BCA protein assay kit (Thermo Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Conditional supernatants from lentivirus-infected CHO cells sorted by FACS were used to purify the FXa-Fc fusion protein using a protein G column (89927, Thermo Fisher). For in vivo testing ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein concentration of the extracts was estimated based on tissue weight or normalized using Pierce Coomassie Protein Assay Kit (ThermoFisher Scientific). Co-immunoprecipitation (coIP ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations of all tissue fractions and TL were determined using the Bicinchonic acid assay (BCA Protein Assay Kit, Thermo Scientific). Bovine serum albumin was used as a protein standard ...
-
bioRxiv - Neuroscience 2020Quote: ... Total protein concentration was determined using the Bio-Rad Bradford protein assay kit according to the manufacturer’s instructions (ThermoFisher Scientific, #23225). An adapted BODIPY (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein samples (10 μg/well) were loaded to NuPAGE Novex 4–12% Bis-Tris protein gel (ThermoFisher Scientific, Carlsbad, CA, NP0336BOX) and transferred onto polyvinylidene difluoride (PVDF ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were lysed in Radioimmunoprecipitation assay (RIPA) buffer and protein concentration was determined by using Micro BCA TM protein assay kit (Thermo Fisher). Protein extracts were loaded onto SDS poly-acrylamide gels and run in 1x Tris-glycine SDS buffer ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lysates were cleared by centrifugation and protein concentrations were determined using the Pierce 660 nm Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of protein were diluted in Laemmli sample buffer ...
-
bioRxiv - Microbiology 2021Quote: ... the purified proteins were loaded on an SDS-PAGE and silver stained for protein visualization (Pierce Silver Stain Kit, Thermo Scientific). Coomassie stained SDS-PAGE samples were sent for mass spectrometry analysis.
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were clarified by centrifugation (20000 g, 10 minutes, 4 °C) and protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific). The same amount of protein (1.2 μg ...
-
bioRxiv - Cell Biology 2021Quote: ... The concentration of protein per sample was determined using the Bicinchoninic Acid (BCA) Protein Assay Kit (23228, Pierce/Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: ... alongside Chameleon Duo Protein Ladder (3 μL/lane; LiCOR, 928-60000) or PageRuler Protein Ladder (5 μL/lane; Thermo Scientific, 26616) and run in pre-chilled 1X MOPS Buffer (Thermo ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were pelleted for 15 min at 13 000 xg at 4 °C and supernatants kept for protein quantification (BCA Protein Assay Kit, Thermo Scientific). Equal amounts of cellular proteins were resolved on 10% or 12% sodium dodecyl sulfate – polyacrylamide gels (SDS-PAGE ...
-
bioRxiv - Cell Biology 2021Quote: ... POS preparations were washed in PBS and resuspended in medium and protein content was measured (BCA Protein Assay Kit, Thermo Scientific) and in parallel ...
-
bioRxiv - Cell Biology 2021Quote: ... The final pellet was resuspended in 200 μL of extraction buffer and the mitochondrial protein concentration was determined with the Pierce BCA Protein Assay Kit (Thermo Scientific). For the Proteinase K accessibility assay ...
-
bioRxiv - Neuroscience 2022Quote: ... and human brain tissues were prepared using a RIPA buffer and the protein contents were measured using a PierceTM BCA Protein Assay Kit (Thermo Scientific). Proteins were separated on 12% SDS-PAGE gradient gel and transferred onto a nitrocellulose membrane ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cellular debris was pelleted for 15 minutes at 4 °C and protein concentration was quantitated with the Coomassie (Bradford) Protein Assay (ThermoFisher Scientific). A total of 15μg (organoids ...
-
bioRxiv - Microbiology 2022Quote: Our metabolic click-labelling was based on the Click-iT HPG Alexa Fluor protein Synthesis Assay Kit (Click-iT HPG Alexa Fluor Protein Synthesis Assay Kit, ThermoFisher C10428) strategy ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein synthesis was measured using Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10456; Life Technologies) per manufacturer’s instructions.
-
bioRxiv - Neuroscience 2022Quote: ... 4 μg of purified protein was mixed with DTT and analysed using a NuPAGE™ 4-12% Bis-Tris mini protein gel (ThermoFisher) as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: Protein concentration of control and stimulated myotube whole cell lysates was determined using Pierce™ BCA protein assay kit (ThermoFisher Scientific) following the manufacturer’s instructions as described before [37] ...
-
bioRxiv - Bioengineering 2022Quote: ... OMV coating on AuNP was determined by quantifying the protein content in OMV using a CBQCA protein quantitation kit (ThermoFisher Scientific). For stability studies of OMV coated AuNP ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged and the supernatants were transferred to new tubes for protein quantification using the Qubit Protein Assay Kit® fluorometric (Invitrogen), following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... The supernatants were collected and the total protein concentration was determined by BCA protein assays (Thermo Scientific™ Pierce™, # 23225). The proteins (20-30 μg ...
-
bioRxiv - Immunology 2022Quote: Homopropargylglycine (HPG) protein synthesis was measured using the Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit from Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: Expression plasmids encoding His-tagged SARS-CoV-2 RBD (residues 319 to 541 of SARS-CoV-2 S protein) or S protein (with a C-terminal trimerization domain) were cloned into pCEP4 vector and transfected into Expi293F (ThermoFisher Scientific) and the proteins expressed for 7 days at 37°C (Rouet et al. ...
-
bioRxiv - Neuroscience 2022Quote: Cell culture supernatants from cerebral cortex neurons were centrifuged at 10,000 g and 4°C and subjected either to protein precipitation or to immunoprecipitation using Reelin antibody G10 and Dynabeads Protein G (ThermoFisher Scientific).
-
bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
-
bioRxiv - Pathology 2022Quote: ... Cell lysates were centrifuged and the protein concentration of the supernatants were quantitated using BCA protein assay kit (Thermo Scientific, 23227). Cell extracts (800 μg ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 60°C for 15 min and protein concentration was determined by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific). The protein mixture (30∼50 µg ...
-
bioRxiv - Neuroscience 2022Quote: ... the protein concentration in the supernatant was determined with a BCA Protein Assay kit (Pierce, Thermo Fisher Scientific, Rockford, IL, USA). To each protein sample (20 μg) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Pull-down of RNA– protein complexes was performed using a Pierce™ Magnetic RNA-Protein Pull-Down Kit (20164; Thermo Scientific). In brief ...
-
bioRxiv - Microbiology 2022Quote: ... At least 400 μg of total protein were incubated overnight with the designated antibody and then with protein G-coupled magnetic beads (Life Technologies) for 1 h ...
-
bioRxiv - Genetics 2022Quote: ... alongside Chameleon Duo Protein Ladder (3 μL/lane; LiCOR, 928-60000) or PageRuler Protein Ladder (5 μL/lane; Thermo Scientific, 26616) and run in pre-chilled 1X MOPS Buffer (Thermo ...
-
bioRxiv - Genetics 2022Quote: ... One μL from each sample was used to quantify protein amounts in using BCA protein assay kit (Thermo Fisher Scientific, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...
-
bioRxiv - Systems Biology 2022Quote: ... the protein concentration was determined using the Pierce BCA protein Assay kit in combination with a NanoDrop 2000 photospectrometer (Thermo Scientific).
-
bioRxiv - Cancer Biology 2022Quote: ... An RNA–protein pull-down assay was performed using a Pierce Magnetic RNA–protein pull-down kit (20164, Thermo Fisher Scientific). In brief ...
-
bioRxiv - Cell Biology 2022Quote: For extraction of membrane and cytosol proteins the cells were lysed according to the Mem-PER Plus Membrane Protein Kit protocol (#89842, ThermoFisher Scientific). The supernatants were kept frozen in −80 °C until total protein quantification ...
-
bioRxiv - Neuroscience 2020Quote: ... Biochemical analyses were performed on water-homogenised tissues (50 mg/mL) and protein content determined (BCA protein assay, Thermo Fisher #23227) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... The amount of total protein in each lane was determined by using the No-Stain Protein Labeling Reagent (Thermo Fisher Scientific). Imaging for puromycin and total protein signals was performed on a FUSION FX7 EDGE Imaging System (Witec AG) ...
-
bioRxiv - Microbiology 2020Quote: ... Both viral RNA and protein were isolated from these PBMCs at the same day using Ambion Paris RNA and protein extraction kit (ThermoFisher Scientific) and stored in −80 °C until use ...
-
bioRxiv - Microbiology 2020Quote: ... 20 min) and the total protein concentration of the cleared lysates was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific) according to manufacturer’s instructions ...