Labshake search
Citations for Thermo Fisher :
6301 - 6350 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... before membranes were dried overnight 2 h at room temperature and proteins stained with Sypro Ruby Protein Blot Stain (ThermoFisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Lysates were spun down at 10,000 RPM and supernatant collected for protein quantification using a Pierce™ BCA Protein Assay Kit (ThermoFisher-23225). NuPAGE™ 4% to 12% ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples of the resuspended protein and the streptavidin bead-bound protein were prepared in NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) supplemented with ꞵ-mercaptoethanol (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay and 200-800 µg of proteins were loaded on Dynabeads Protein G for Immunoprecipitation (ThermoFisher Scientific); the beads were previously incubated for 10 min on a wheel at room temperature with PBS-Tween-20 0.01% supplemented or not with 2.5-5 µg of MIC60 ...
-
bioRxiv - Biochemistry 2024Quote: ... Efficacy of the process and final protein purity was assessed by SDS-PAGE using NuPAGE 4-12 % Bis-Tris Midi Protein Gels (ThermoFisher Scientific) and mass spectrometry ...
-
bioRxiv - Molecular Biology 2024Quote: Protein was isolated from cells as described (Rüegsegger et al., 2001) and quantified by BCA protein assay (#23225, Thermo Fisher Scientific). 40 µg of protein was separated on 10% surePAGE Bis-Tris gels in MOPS running buUer (#M00665 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were normalized to total worm extract protein using Pierce™ BCA Protein Assay Kit (Thermo Scientific™; catalog no. 23225). Free fatty acid content were determined by measuring absorbance at 570 nm using BMG Labtech CLARIOstar Plus plate reader.
-
bioRxiv - Molecular Biology 2024Quote: ... the lysates were centrifuged at 16,400 ×g for 15 min at 4 °C and the protein concentration in the supernatants was measured using a Pierce BCA protein assay kit (ThermoFisher Scientific). Total protein (10 µg ...
-
bioRxiv - Molecular Biology 2024Quote: ... The EPR signal intensities were normalized to the protein concentrations of the samples as determined by BCA protein assay kit (Thermofisher ScientificTM).
-
bioRxiv - Microbiology 2024Quote: ... for standard protein analysis or to native PAGE using a NativePAGETM 3‒12% mini protein gel and a Sample Prep Kit (Thermo Fisher) for analysis of the heptamerization of PA28γ ...
-
bioRxiv - Molecular Biology 2024Quote: ... The lysate was cleared by centrifugation at 21 130 x g at 4 °C for 30 min and protein content quantified using the Pierce BCA protein assay kit (ThermoFisher Scientfic). Twenty µg of the lysate ...
-
bioRxiv - Cell Biology 2024Quote: ... The obtained protein lysate was subsequently used for Co-IP of the Prm2-Septin12 protein complex by Dynabeads Protein G Immunoprecipitation kit (10007D, Invitrogen, USA). Magnetic beads were conjugated with anti-protamine 2 antibody (Mab-Hup2B ...
-
bioRxiv - Genetics 2024Quote: ... protein levels were normalized by total protein amount in the same lane as determined by Novex Reversible Membrane Protein Stain (Thermo Scientific) performed prior to blocking.
-
bioRxiv - Cell Biology 2024Quote: ... The instrument was calibrated by addition of 15 µl of protein standards from Native Mark Unstained Protein Standard (Thermo Fisher LC0725) in buffer A (10 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Protein concentrations of the soluble cellular protein and 100x concentrated secreted protein samples were quantified against Bovine Serum Albumin (BSA) standards using the Pierce Coomassie Protein Assay kit (#23236, ThermoFisher Scientific). The assays were performed in 96-well transparent plates containing 150 μl of diluted protein sample or 0 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... We purchased B-PER™ Complete Bacterial Protein Extraction Reagent and Pierce™ 660 nm Protein Assay Reagent from Thermo Fisher Scientific (USA) ...
-
bioRxiv - Cell Biology 2024Quote: General protein synthesis levels were measured using the Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit (Invitrogen, C10456). After 48 h of siRNA transfection ...
-
bioRxiv - Neuroscience 2024Quote: ... supernatants were removed and then protein pellets were dissolved in mammalian protein extraction reagent (M-PER, Thermo Fisher Scientific, Waltham, MA), containing 1% Halt Protease and Phosphatase Inhibitor cocktail and 1% EDTA (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... and/or crude mitochondrial fractions were first normalized for total protein content via Pierce BCA Protein assay (Thermo Fisher Scientifc 23225). Samples were resolved by SDS-PAGE and transferred to nitrocellulose membranes ...
-
bioRxiv - Molecular Biology 2021Quote: The cDNA encoding the GADD34 protein with a deletion of the amino acids 1-240 was cloned into the pRSET A vector (Thermo Fisher Scientific, Cat. No. V35120) by In-Fusion cloning (Takara ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cleaved Tn5 was eluted in ~1 mL fractions and Tn5 elution efficiency was monitored by the Pierce™ Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific cat# 23200). Fractions with the strongest absorbance were pooled and dialyzed versus two changes of 2 L of 2X Tn5 dialysis buffer (100 mM HEPES-KOH at pH 7.2 ...
-
Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... and rocked end-over-end at 4°C for 1 hour with 10 μl prewashed Dynabeads Protein A (Thermo Fisher Scientific, Cat. #10001D, for rabbit) or Protein G (Thermo Fisher Scientific ...
-
bioRxiv - Pathology 2022Quote: Cells were transfected with the GST fusion protein construct or the BRET-based cAMP construct (1 μg) using Lipofectamine 2000 (Invitrogen Life Technologies, Saint Aubin, France) in Opti-MEM medium.
-
bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
-
bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... The bead-bound biotinylated proteins were analyzed by Western blotting using HRP-streptavidin diluted 1:40 000 in 3% BSA/TBST (Thermo Fisher Scientific, Rockford, IL, USA) and identified by mass spectrometry.
-
bioRxiv - Immunology 2024Quote: ... Proteins were transferred from the gel onto a nitrocellulose membrane using an electric current and fixed to the nitrocellulose and probed with antibodies against the target proteins (anti-PD and anti-ubiquitin; Invitrogen Ubi-1, Cat #13-1600). Bound antibody was revealed with horseradish peroxidase-conjugated rabbit anti-mouse antibody using enhanced chemiluminescence (Amersham Biosciences).
-
bioRxiv - Cancer Biology 2021Quote: ... Final sEVs pellet was resuspended in 100 μL of PBS and the protein content was measured by bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). The NS500 nanoparticle characterization system (NanoSight ...
-
bioRxiv - Cell Biology 2020Quote: ... The resultant mixture was centrifuged at 14,000 g and the supernatant was used to estimate the total protein by using Pierce™ BCA Protein Assay Kit (Thermo scientific). Equal quantity of protein (300 μg ...
-
bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
-
bioRxiv - Cell Biology 2020Quote: ... Membrane pellets were gently resuspended in 100 μl SEM and protein content was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) to normalize total protein concentration across samples ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein content of preovulatory follicles from eCG-injected mice is ~6 μg (Pierce BCA protein assay kit, 23225, Thermo Fisher Scientific). The protein content of follicles (~320-400 μm diameter ...
-
bioRxiv - Cell Biology 2019Quote: ... anti-TAC102 antibody is crosslinked to protein A / protein G beads from the Pierce™ Crosslink Magnetic IP kit (Thermo Scientific™). A mitochondrial enriched fraction is prepared by digitonin fractionation with 100 ml of procyclic form parasites (cell line ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentrations in the supernatants were measured using the bicinchoninic acid assay (Pierce BCA Protein Assay Reagent, Thermo Scientific, Waltham, MA, USA). Equal amount of protein lysates (40 mg ...
-
bioRxiv - Cell Biology 2020Quote: ... TAG amounts were normalized to the total protein amounts in each sample using a Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific). The data were analyzed using a Student’s t test.
-
bioRxiv - Microbiology 2020Quote: The proteins from cell lysates and isolated mitochondria were analysed by separating 10-40 μg of protein (concentrations determined using Pierce BCA Protein Assay kit, Thermo Fisher Scientific), by SDS-PAGE ...
-
bioRxiv - Microbiology 2022Quote: ... supernatants were stored at - 80°C and protein concentrations were further determined using the Pierce™ BCA Protein Assay Kit (23225, ThermoFisher Scientific).
-
bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated by SDS-PAGE in Bio-Rad mini-protean® Tetra system using PageRuler prestained protein ladder (#26616, Thermo scientific) as molecular-mass standards ...
-
bioRxiv - Molecular Biology 2021Quote: ... the P2 fraction was brought up in 400uL of PBS and protein concentrations were measured using the QuBit Protein Assay Kit (Thermo Fisher, Q33211). Samples were precleared with 15mg of DEPC treated (to inactivate RNAse ...
-
bioRxiv - Molecular Biology 2020Quote: ... PIWIL1 and PIWIL3 proteins were immunopurified using anti-MARWI (1A5) and anti-Hamster PIWIL3 (3E12) immobilized on Dynabeads protein G (Life Technologies, 10004D) and anti-Mouse IgG (IBL ...
-
bioRxiv - Molecular Biology 2021Quote: ... 12 µg of protein per sample was used for gel electrophoresis with a NuPAGE™ 4-12% Mini Protein Gel (Invitrogen, NP0323) in a Mini Gel Tank (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein concentration of the isolated exosomes was detected by using a total exosome RNA and Protein Isolation Kit (Thermo Fisher Scientific) and a BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... SOD2-V5 protein in 125 or 250 μg lysate was immunoprecipitated with anti-V5 antibody and Dynabeads Protein G (Thermo Fisher Scientific), and then immunoblotted with 25 μg input samples.
-
CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... The molecular migration position of transferred proteins was compared to the PageRuler Prestain Protein Ladder 10 to 170 kDa (Thermo Fisher Scientific). Signal quantification was performed using Image J software (NIH).
-
bioRxiv - Neuroscience 2019Quote: ... The signal was normalized to the amount of protein in the sample as determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer instructions.
-
bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 10,000 x g for 30 min at 4 °C before supernatants were collected and protein content was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific) following the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2019Quote: ... to remove RNA and DNA and then incubated overnight with the appropriate antibody and then incubated overnight with a 50:50 mixture of protein A and protein G Dynabeads (Thermo Fisher Scientific). Beads were washed three times with nondenaturing lysis buffer (20 mM Tris-HCl ...
-
bioRxiv - Genetics 2019Quote: ... The molecular migration position of transferred proteins was compared to the PageRuler Prestain Protein Ladder 10 to 170 kDa (Thermo Fisher Scientific). Signals were detected using the Chemidoc XRS+ imaging system (Biorad ...
-
bioRxiv - Cancer Biology 2019Quote: ... we transferred the supernatant to a clean tube and determine the protein concentration using the EZQ® Protein Quantitation Kit (Cat. no. R33200,Thermo Scientific) or another method ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: We quantified the protein content in the clarified lysates of the damselflies using Pierce™ BCA protein assay kit (Thermo Fisher Scientific). We used the Bovine Serum Albumin (BSA ...