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Citations for Thermo Fisher :
6051 - 6100 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... of NS2B and N-terminal portion (amino acid 1 to 185) of NS3 were amplified and inserted into the multiple cloning site of protein expression plasmid pTrcHisA (Thermo Fisher Scientific, MA, USA) through restriction sites XhoI and EcoRI ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific) followed by blotting onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2020Quote: ... Then proteins were separated by electrophoresis in 4%–12% Bis-Tris Protein Gels and transferred to polyvinylidene difluoride membrane (Thermo Scientific). The membranes were blocked in 5% dry milk in 0.1% Tween-20 in PBS and detected with the indicated antibodies ...
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bioRxiv - Cell Biology 2020Quote: Protein concentration of cell lysates was determined using the Pierce™ BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... forebrain samples were homogenized with lysis buffer and protein concentrations determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of total protein were incubated with TX antibodies (generated in our lab[23] ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 µg of total protein was loaded into NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific, #NP0321BOX). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit and Trans-Blot© Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... The supernatant was stored at −20°C for protein quantification performed using Pierce ™ BCA Protein Assay Kit (Thermo Fisher, 23227).
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bioRxiv - Cell Biology 2020Quote: ... the sample was recovered from the Amicon tube into a new microfuge tube and protein concentration was determined using bicinchoninic acid (BCA) protein assay (Thermo Scientific). Proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (1:25 enzyme/substrate ratio) ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
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bioRxiv - Genetics 2021Quote: ... Supernatant was transferred to new a 1.5 microcentrifuge tube on ice and protein quantification was performed using a Qubit protein assay kit (Thermo Fisher Q33212) and a Qubit 4 fluorometer (Q33238).
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bioRxiv - Immunology 2020Quote: ... Lysate volumes were subsequently adjusted for equal protein levels following BCA assay (23227, Pierce BCA protein assay kit, Thermo Fisher, USA), and GTP-bound Rac1 levels were assessed according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... The amount of ATP was then determined and normalized with the protein level measured by BCA protein assay (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... and then 10 μg of protein per sample was mixed with SDS and reducing reagent and loaded onto NuPAGE Bis-Tris protein gels (Invitrogen, NP0322) after boiling at 70 °C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Final proteins concentration in the NE extracts was determined using the colorimetric Pierce BCA protein assay kit (Thermo Scientific, Waltham, MA). Pellets were stored at −80° C.
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bioRxiv - Molecular Biology 2021Quote: Eluted proteins were separated on a 1.5 mm NuPAGE™ Novex™ 4-12 % Bis-Tris protein gel (Thermo Fisher Scientific) until the running front had migrated roughly 2 cm ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted proteins were analyzed on a NuPAGE 4-12% Bis-Tris 1.0 mm Mini Protein Gel (Invitrogen, cat. No. NP0321) and stained with Instant Blue (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Genomics 2020Quote: ... diluted chromatin was incubated overnight at 4°C with Protein A and Protein G Dynabeads (ThermoFisher Scientific 10002D, 10004D; 25µL each) and indicated antibodies (Key Resources Table) ...
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bioRxiv - Bioengineering 2020Quote: ... the final working concentration of protein was found to be 100 μg/ml using a BCA Protein Assay kit (Thermo Scientific). Purity of the protein was confirmed by SDS-PAGE followed silver staining of the gel ...
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bioRxiv - Plant Biology 2020Quote: ... Total crude protein was isolated from 100 mg of plant leaves using P-PER plant protein extraction kit (Thermo scientific: 89803) and the western blot was carried out by electro blotting the 20µg of total crude protein onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2019Quote: Synaptosomes were prepared using the Procedure for Synaptic Protein Extraction from Neuronal Tissue” and Syn-PER Synaptic Protein Extraction Reagent (Thermo Fisher, #87793 ...
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bioRxiv - Cancer Biology 2019Quote: ... the protein sample was reacted with biotin-alkyne using the Click-iT Protein Reaction Buffer Kit (Cat. no. C10276, Thermo Scientific) following protocols in the instruction sheet ...
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bioRxiv - Immunology 2020Quote: ... Lysates were boiled and sonicated (15 cycles of 30s on/30s off) and protein concentrations determined by EZQ® Protein Quantitation Kit (Invitrogen). Lysates were then alkylated with iodoacetamide (IAA ...
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bioRxiv - Biophysics 2019Quote: ... the concentration of virus was estimated as we have reported previously (15) by measuring the viral protein concentration using a BCA protein concentration assay kit (ThermoFisher Scientific) and then dividing the measured protein concentration by the average total protein molecular weight per viral particle (1.8 × 108 Da ...
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bioRxiv - Cell Biology 2019Quote: ... The concentration of protein on beads and whole cell extracts were measured using the BCA assay (Pierce BCA protein assay kit, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... Afterwards the supernatant was transferred into a fresh tube and the protein concentration was determined using a BCA protein assay kit (Thermo Fisher scientific ...
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bioRxiv - Cell Biology 2019Quote: ... pH8.0) added of protease inhibitors and protein lysates were quantified by Pierce™ BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Lysates were separated on SDS-PAGE and wet-transferred to nitrocellulose membranes (#NBA083C ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentration was determined using Pierce™ BCA Protein Assay Kit according to the manufacture’s manual (ThermoFisher Scientific, Catalog number: 23227). 40µg of protein from each sample was loaded onto the 4– 15% Mini-PROTEAN® TGX™ Gel (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... The immune-reactive proteins were detected using the protein bands were visualized using SuperSignalTM West Dura Extended Duration Substrate (ThermoFisher Scientific) and visualized on Image Quant LAS 500 machine (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Lipid peroxidation levels were normalised against total protein quantitated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, United States).
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bioRxiv - Genetics 2020Quote: ... Protein quantification of the supernatant was performed using the Pierce™ BCA protein assay kit (Thermo-Fisher Scientific, Rockford, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... liver tissues were lysed in RIPA buffer and total protein concentrations were determined by MicroBCA protein assay kit (Thermo Scientific, 23235) according to the manufacturer’s instructions and infinite 200 PRO microplate reader (Tecan) ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were cleared by centrifugation and protein-containing supernatants were collected into fresh tubes and quantified with the Pierce BCA protein assay kit (Thermo Scientific) using a two-fold dilution curve of BSA (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Evaluation of protein expression was performed by running 20 μg of total protein lysate on NuPage Bis Tris 4%-12% gels (Invitrogen, USA). Proteins were transferred using iBlot dry transfer system (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... purified NHR2 protein was labeled either with the dye Alexa Fluor® 488 (NHS Ester Protein Labeling kit, ThermoFisher Scientific, USA) or Monolith NT™ (Protein Labeling Kit Blue-NHS ...
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bioRxiv - Bioengineering 2021Quote: ... The supernatants were transferred into new tubes and protein concentration was measured using the Pierce 660 nm Protein Assay (ThermoFisher Scientific). Proteins were resolved on 4 – 20% SDS-PAGE gels (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: Total adsorbed protein in a defined volume was determined by measuring protein concentration with the Pierce 660nm assay (ThermoFisher Scientific, Inc.) calibrated against a bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... Pellets were resuspended in PBS and semEV concentrations were estimated by BSA protein kit (Thermo Fisher, Pierce BCA Protein Assay Kit) and adjusted to 2mg/mL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cortical lysates were prepared according to the assay type and protein concentration was determined using BCA Protein Assay Kit (ThermoFisher Scientific) prior to the assays ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were centrifuged at 4 °C for 10 minutes at 16000 g to remove cell debris and protein concentration of the lysate was measured using Pierce BCA Protein Assay Kit (Thermo Scientific). Equal amounts (10 µg ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in Radioimmunoprecipitation assay (RIPA) buffer and protein concentration was determined by using Micro BCA TM protein assay kit (Thermo Fisher). Western blot was performed as described previously (32) ...
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bioRxiv - Cell Biology 2021Quote: Equal amounts or fractions of indicated protein lysates were loaded onto NuPage 4-12% Bis-Tris Protein Gels (Invitrogen, Waltham, MA), and then subsequently immunoblotted with the primary antibodies listed in Supplementary Table 2 ...
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bioRxiv - Physiology 2020Quote: ... 8 mg of the soluble protein fractions were pre-cleared with 200 μL of protein G-magnetic Dynabeads (Thermo Fisher Scientific) for 1 hr ...
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bioRxiv - Biochemistry 2020Quote: A 5-mL HiTrap™ Protein G HP column (Cytiva) was equilibrated with 10 CVs of Protein G IgG Binding Buffer (pH 5.0; Thermo Fisher Scientific). Spent medium containing CR3022 mAb was diluted with binding buffer at a 1:1 volume ratio and filtered using a 0.22 μm stericup ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentrations of the supernatants were determined using a Pierce BCA protein assay kit (Thermo Scientific, catalog # 23225, lot # SJ256254) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Segregation and enrichment of proteins from different cellular compartments were performed using a Thermo Scientific Subcellular Protein Fractionation Kit (78840, Thermo Fisher) followed the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... A small volume of each sample was used to immediately measure protein concentration with the Pierce BCA Protein Assay kit (ThermoFisher; 23227). The rest was combined with 6X loading dye (375mM Tris pH 6.8 ...