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Citations for Thermo Fisher :
6151 - 6200 of 10000+ citations for Monkey Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Luminescence readings for each sample were compared to an ATP standard curve and normalized to the total amount of protein determined using the Pierce BCA Protein Assay Kit (23225, Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and the protein assay was performed using the Pierce BCA Protein Assay kit (Cat#23225, ThermoFisher Scientific). The supernatants from different samples were adjusted to the same protein concentration of protein and the same volume of the supernatants were subjected to acetylcholine assay using QuickDetectTM Acetylcholine (ACh ...
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bioRxiv - Cell Biology 2021Quote: ... The volume corresponding to 200–300 μg of protein was incubated with the corresponding antibody and Dynabeads Protein G (Life Technologies) overnight at 4°C to form immunocomplexes ...
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bioRxiv - Cell Biology 2021Quote: ... The volume corresponding to 60–100 μg of protein was incubated with the corresponding antibody and Dynabeads Protein G (Life Technologies) overnight at 4°C to form immunocomplexes ...
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bioRxiv - Physiology 2021Quote: ... protein content was measured from 100 μl of diluted samples with Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, IL, USA) and lipid content was analyzed by hydrolysis according to an internal method adapted from NF ISO 6492 by Artemis Laboratory (Janzé ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysed cell suspensions were quantified for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Loughborough, UK). Working solutions were made up to 0.5 μg/μl with RIPA and 2x Concentrate Laemmli Sample Buffer (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: The whole extract and the cytosolic and synaptosome protein fractions were collected using the Syn-PER™ Synaptic Protein Extraction Reagent (ThermoFisher) following manufacture instructions ...
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bioRxiv - Immunology 2020Quote: ... Punch biopsy skin samples were obtained from XenoSCID and protein extracted and homogenised using M-PER mammalian protein extraction reagent (Thermo Scientific) and two 7mm metal beads ...
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bioRxiv - Neuroscience 2021Quote: ... PS2 or PSD fractions were quantified for protein concentration using the Qubit® Protein Assay Kit (Thermo Scientific Rockford, IL, USA).
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bioRxiv - Biochemistry 2021Quote: ... The purity of the protein was checked by SDS-PAGE and the concentration was determined using a Pierce BCA protein assay kit (Thermo Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... subcellular protein fractionation was performed as described in the Subcellular protein fractionation kit for cultured cells (Thermo Scientific; Cat. No. 78840). Fractions were stored in −80°C per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were cleared by centrifugation at 16,000 × g for 5 min at 4°C and protein concentration was determined using the Pierce BCA protein assay kit (Thermo Scientific). Cell lysates were mixed with Laemmli sample buffer (Alfa Aesar ...
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bioRxiv - Immunology 2022Quote: ... The Protein G resin was washed four times with PBS and the IgG was eluted with Protein G Elution Buffer (Thermo Scientific) and neutralized with 1 M Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Data were analyzed using the Agilent Seahorse Wave Software and normalized to total protein context per well using the Pierce Rapid Gold BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Physiology 2022Quote: ... 20 mg of pulverized tissue were dissolved in the assay buffer from the kit as suggested by the manufacturer and normalized to protein content by Pierce™ BCA Protein Assay Kit (ThermoFisher). 250 μg of protein was used for each reaction ...
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bioRxiv - Biophysics 2019Quote: ... Total protein concentration of the extracts was determined using the Pierce™ Coomassie Protein Assay Kit (ThermoFisher Scientific, Porto Salvo, Portugal).
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bioRxiv - Molecular Biology 2019Quote: ... bakeri worm lysates or EVs were carried out with rat polyclonal anti-exWAGO antibody (raised against full length protein) or rat IgG (control) using protein L magnetic beads (Fisher Scientific). Equivalent amounts of the input ...
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bioRxiv - Molecular Biology 2019Quote: ... with the addition of 30 µl of protein A (for anti-H3K9me) or protein G (for anti-FLAG) Dynabeads (Thermo Fisher). Library preparation was performed as described in (Homer et al. ...
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bioRxiv - Biochemistry 2020Quote: Protein concentration in the cell extract was measured using a Pierce BCA protein assay kit (product 23227; Thermo Scientific, Rockford, IL).
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bioRxiv - Evolutionary Biology 2019Quote: ... and each sample was standardized to protein concentration determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2019Quote: ... The protein concentration was measured on the cleared lysates with PierceTM BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). After diluting the lysates in RIPA and Laemmli buffer ...
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bioRxiv - Plant Biology 2019Quote: ... The supernatant was filtered through a 0.22 μm Millipore Express PES membrane and proteins were quantified using PierceTM BCA protein assay kit (Thermo Fisher Scientific). Small aliquots were taken as input samples whereas the remaining parts were used for co-IP ...
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bioRxiv - Cell Biology 2019Quote: Cells were lysed in 1x Laemmli Buffer pre-warmed to 95°C and the total protein concentration was normalised using the Pierce BCA Protein Assay (Cat. # 23250; ThermoFisher Scientific). Proteins (10µg/sample ...
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bioRxiv - Cancer Biology 2019Quote: ... mAb-phage complexes were captured by magnetic beads (20µL of protein A-coated and 20 µL of protein G-coated, catalog numbers 10002D and 10004D, Invitrogen, Carlsbad, CA) for 4 hours at 4°C with rotation and processed using the Agilent Bravo liquid handling system (Agilent Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was transferred to a new tube for protein quantification with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23225). 40 μg of protein was separated by NuPAGE 4-12% Bis-Tris Protein Gels (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentrations of the solutions were measured using a bicinchoninic acid assay (Pierce™ BCA Protein Assay Kit; Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... Supernatant (2 mg of proteins) was incubated with 2 μg of target antibodies and 20 μl of Protein A/G Plus Ultralink Resin (Thermo Scientific) for 4 hours at +4°C ...
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bioRxiv - Evolutionary Biology 2020Quote: Expression of BRCA2 at protein level was confirmed by western blotting using NuPAGE® Large Protein Blotting Kit (Invitrogen, CAT# LP0001) following the protocol supplied by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... The lysate was incubated for 2 hours in a rotating wheel at 4°C with Dynabeads Protein A (#10002D Dynabeads Protein A, ThermoFisher Scientific) as a preclearing step ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified protein quality was assessed by SDS-PAGE/Coomassie staining to be >95% pure and protein concentration was quantified with PierceTM BCA Protein Assay Kit (ThermoFisher Scientific). Protein was stored at - 80°C until further use.
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bioRxiv - Biochemistry 2020Quote: ... and lysates containing 30 µg of protein were electrophoresed on a NuPageTM 4–12% Bis-Tris Protein gel (Thermo Fisher Scientific). Proteins were transferred to a PVDF membrane (Amersham) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cytokine measurements for lung homogenate samples were corrected for total protein concentration using a Pierce™ BCA Protein Assay Kit (ThermoFisher).
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bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
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bioRxiv - Microbiology 2020Quote: ... The total protein concentration in each sample supernatant was quantified using the bicinchoninic acid (BCA) method (Pierce BCA Protein Assay Kit; Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: Conventional interactome analysis of the IP samples was performed as described before [57] with the following changes: The enriched proteins were separated by SDS-PAGE with a 4-12% Bis-Tris protein gel (NuPAGE, Thermo Scientific) and stained with Colloidal Blue Staining Kit (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was measured with the BCA Protein Assay Kit (Pierce Biotechnology) and equal amounts of protein were separated on 4–12% Tris-Glycine Gels (Thermo Scientific) and transferred onto Immobilon-P PVDF membranes (Merck Millipore) ...
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bioRxiv - Biochemistry 2021Quote: ... Total protein concentration was determined using a bicinchoninic acid (BCA) assay from the Pierce™ BCA Protein Assay Kit (Thermo Scientific). The fluorescence spectra of the EYFP samples were measured with a Varioskan LUX Multimode Microplate Reader (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... Lysates were cleared by centrifugation at 20,000 g for 15 min at 4°C then protein concentrations determined using Coomassie Protein Assay Kit (Thermo Scientific, UK). For each sample ...
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bioRxiv - Cell Biology 2019Quote: ... The protein concentration of the conditioned medium was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, cat#23225), and medium volumes ...
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bioRxiv - Neuroscience 2019Quote: ... TAG concentrations were normalized to the total protein content as determined by a Micro BCA protein assay kit (ThermoFisher, Cat # 23235). Each assay was done in triplicate and the average TAG level (μg TAG/ μg protein ...
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bioRxiv - Cell Biology 2021Quote: ... The cell pellet was also snap frozen and stored for protein analysis via the manufacturer’s instructions for the Pierce BCA Protein assay kit (Thermo Fisher Scientific) to normalize the NMR data ...
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bioRxiv - Biochemistry 2020Quote: ... The microsomes were resuspended and diluted according to the protein concentration measured in the supernatants using the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Genetics 2021Quote: ... Then concentration of the protein was measured by the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA, Cat. #23227). Samples were stored at -80°C until proteomic preparation.
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bioRxiv - Neuroscience 2020Quote: ... Assay results were normalized to the total protein level in each ON sample as quantified with the Pierce BCA protein assay kit (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation of protein extracts was performed using a standard protocol based on magnetic beads coupled to bacterial protein G (Invitrogen #10003D). 1µg of anti-LRRFIP2 antibodies per 500µg of protein extracts was used ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total proteins were isolated from cells and tissues using the Nuclear and Cytoplasmic Protein Extraction Kit (Thermo Fisher Scientific, Waltham, USA). Approximately 40 μg of protein was separated on 12% SDS-polyacrylamide gel (SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... The optical density at 492 nm was measured to estimate melanin content and normalized to protein concentration as determined by BCA protein determination kit (Thermo Fisher). Plat-E cells ...
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bioRxiv - Cell Biology 2020Quote: ... The concentration of protein crude extract was determined by means of bicinchoninic acid (BCA) protein assay (Thermofisher Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the lysates was measured via BCA assay and biotinylated proteins were immunoprecipitated using Streptavidin magnetic beads (Thermo Fisher). 500μg magnetic beads were incubated with up to 70μg of lysate rotating overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...