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Citations for Thermo Fisher :
6101 - 6150 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h and incubated overnight at 4°C with the following primary antibodies: anti-GFP (Thermo Fisher Scientific, A-11122, 1:1000), anti-TIAR (BD Transduction ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Cell monolayers were then incubated in the presence of secondary antibody (goat anti-rabbit IgG (H+L) Alexa Fluor 488 conjugate (#A11008, Thermo Fisher Scientific, USA), or ...
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bioRxiv - Cell Biology 2024Quote: ... Lipofectamine transfected cells were incubated for 4 h and the medium containing the transfection mixture was then replaced with cortical neuron-conditioned neurobasal medium (Thermo Fisher Scientific, 21103049) (low-density cultures ...
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bioRxiv - Cell Biology 2024Quote: ... sections were incubated with Alexa Flour 488-conjugated goat anti-rabbit IgG (H+L) antibody (1:2000) (Thermo Fisher Scientific, Waltham, MA, USA) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation for 2 h in a donkey anti-rat secondary antiserum (1:750; AlexaFluor-594, Thermo Fisher Scientific, catalog #A-21209). Last ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue sections were then incubated with host-specific fluorophore-conjugated secondary antibodies for 1 h at room temperature and coverslipped using ProLong Gold with DAPI (Molecular Probes, Life Technologies). In some cases ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV-CaMKIIa-EGFP subjects were incubated with Alexa Fluor 594 donkey anti-rabbit IgG (H+L) (Lot #: WD319534, REF: A32754, Invitrogen, Carlsbad, CA USA) for one hour at room temperature.
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bioRxiv - Neuroscience 2024Quote: ... subjects in either the pAAV-CaMKIIa-hM4Di-mCherry or pAAV-CaMKIIa-hM3Dq-mCherry groups were incubated with Alexa Fluor 488 goat anti-rabbit IgG (H+L) (Lot #: 1981125, REF: A11008, Invitrogen, Carlsbad, CA USA) and pAAV-CaMKIIa-EGFP subjects were incubated with Alexa Fluor 594 donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... Blunted DSB ends were ligated to sBLISS adaptors for 16–18 h at 16 °C using the T4 DNA ligase kit (Thermo Fisher Scientific, EL0012). Following washes with CSTX buffer ...
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bioRxiv - Neuroscience 2024Quote: ... 100 ng of DNA was used to perform in vitro transcription for 15 h at 37 °C with the Megascript T7 transcription kit (Thermo Fisher Scientific, AMB13345). 1 U DNase I (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... medium were inoculated with a single isolated colony from a freshly streaked LB agar plate and grown overnight (∼16 h) at 37°C with aeration (225 rpm) in a MaxQ 4000 benchtop incubator (ThermoFisher Scientific, Waltham, MA). Overnight cultures were subcultured 1:50 into LB broth and grown to mid-log phase (OD600 of ∼0.7 ...
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bioRxiv - Plant Biology 2024Quote: ... and GmNAC42-1 were PCR amplified from the cDNA of W82 hairy root treated with WGE (24 h) using specific primers (Table S16) and 2X Phusion Master Mix (ThermoFisher Scientific, MA, USA). The PCR amplicons were cloned into the entry vector pDONR221 using BP clonase (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The resulting cDNA was treated with RNAse H and combined with a custom 4x TaqMan™ Gene Expression Master Mix (Thermo Fisher Scientific) containing primers and a fluorescently labeled hydrolysis probe specific for the gag gene of SIVmac239 (forward primer 5’-GTC TGC GTC ATC TGG TGC ATT C −3’ ...
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bioRxiv - Molecular Biology 2024Quote: ... cerevisiae purified 40 nm VLPs using randomly selected primer from laboratory stock 99.R and RevertAid H Minus Reverse Transcriptase (Thermo Fisher Scientific, USA) performing cDNA synthesis step at 37 °C ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... Brain slices were then incubated for 20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry, 1:1000, Thermo Fisher Scientific, M11217) in PBS-based blocking solution containing 0.1% TX ...
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bioRxiv - Neuroscience 2024Quote: ... Immunohistochemistry to analyze white matter sparing followed a similar procedure but using a Goat anti-NF-H antibody (1:3000; Thermo Fisher PA5- 143591) along with Donkey anti-Goat IgG Alexa Fluor 568 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... monocytes were allowed to adhere to 24-well plates at 5×105 cells/well for 1 h at 37°C in warm RPMI-1640 medium (ThermoFisher Scientific, Waltham, MA). The mean purity of adherent monocytes was 85 % (range ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated 1 h at 37 °C in saturation solution containing the relevant secondary antibody (goat anti-rabbit Alexa 488; Invitrogen, Carlsbad, California, USA). After washing in PBST three times ...
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bioRxiv - Pathology 2024Quote: ... the cells were incubated for 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG (H+L) antibody (Invitrogen, Thermo Fisher Scientific, USA). Nuclear and kinetoplast DNA were stained with DAPI (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... for a period of 16-24 h at 37°C in total volumes of 10 ml in 50 ml Falcon™ centrifuge tubes (Fisher Scientific, UK), using the cell densities and culture conditions described in the MIC protocol ...
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bioRxiv - Immunology 2024Quote: ... the slides were washed and incubated with the following secondary antibodies for 1 h at room temperature: AF488 donkey anti-goat IgG (Thermo Fisher Scientific, A32814), AF647 donkey anti-goat IgG (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... then transferred to 30% sucrose for an additional 16-18 h prior to embedding in Tissue-Plus OCT (Fisher Scientific; 23-730-571) and freezing at -70°C ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for five days with a 10% (v/v) feed added 48 h post-transfection containing 1:1 (v/v) Efficient Feed A (Thermo Fisher Scientific, USA) and Efficient Feed B (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Proximity ligation was performed by in-situ phosphorylation of adaptors for 1 h at 37 °C (1× T4 DNA ligase buffer with ATP, 1 U PNK (Thermo Fisher Scientific, EK0032)) and in-situ ligation for 4 h at room temperature (1× T4 DNA ligase buffer ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in primary antibodies in 3% serum in PBS-T for up to 48 h at 4°C: anti-phospho-tau (ser202, thr205) (clone AT8) (ThermoFisher, MN1020; 1:150), anti-GFAP (Millipore-Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor 488 (#A-11008) and goat anti-mouse IgG (H+L) Alexa Fluor 647 (#A-21235) were from Invitrogen (Carlsbad, CA, USA).
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bioRxiv - Biophysics 2024Quote: ... and incubating them with the secondary antibody goat anti-rat IgG (H+L) AlexaFluor 546 (1:400, 10729174, Fisher Scientific GmbH, Schwerte, Germany) in combination with the conjugated antibody mouse anti-ZO1 AlexaFluor 488 ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed and further incubated with the secondary antibody Goat anti-Rabbit IgG (H+L) Alexa Fluor™488 (cat#A-11008, Invitrogen, 1:1000), or Goat anti-Rabbit IgG (H+L ...
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DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptidesbioRxiv - Biophysics 2024Quote: ... the supernatant of cell lysates clarified by centrifugation (8, 184 x g, 1 h) was then affinity purified using Pierce™ anti-DYKDDDDK affinity resin (ThermoFisher Scientific, A36801) and eluted with 3 x Flag peptide (GenScript ...
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bioRxiv - Cell Biology 2024Quote: ... together with 50 µl ACN to yield a final ACN concentration of approximately 25% (v/v) for 1 h at room temperature before quenching the reaction with hydroxylamine (Thermo Fisher Scientific 90115) to a final concentration of 0.2% (v/v) ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid membranes were washed 3x with TBST and incubated with FLAG Tag primary antibody for 1 h at room temperature and detected using SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific #34076).
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bioRxiv - Cell Biology 2024Quote: ... Tissues were then incubated in darkness overnight (18-24 h) with 0.5% phalloidin conjugated to AlexaFluor 488 (A12379; ThermoFisher Scientific, Toronto, ON, Canada) in PBSTx to stain F-actin ...
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bioRxiv - Cell Biology 2024Quote: ... sections were incubated with Alexa Flour 647-conjugated goat anti-rat IgG (H+L) antibody (1:2000) (Thermo Fisher Scientific, Waltham, MA, USA) and DAPI solution (20μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... were diluted in blocking buffer containing Hoechst 33342 nuclear stain (1:1000) and incubated on tissue sections at 37°C for 1 h (Abcam, Cambridge, UK; Thermo Fisher, Waltham, MA). Representative 20X images were taken of each tissue section on a Nikon Spinning Disc confocal microscope.
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bioRxiv - Cancer Biology 2024Quote: ... Tissue chips were blocked with 5% BSA for 1 h at room temperature and subsequently incubated with TLL1 primary antibody (Invitrogen, Cat#PA5-24246) (diluted at 1:50 ...
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bioRxiv - Biochemistry 2024Quote: ... The following fluorescence-conjugated secondary antibodies were used: Donkey anti-Rabbit IgG (H+L) Alexa Fluor 488 (Thermo Scientific, A21206, ICC 1:500) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2024Quote: ... a 30 min with secondary antibody diluted at 1:200 in blocking media was performed (Alexa Fluor 488 (AF-488) Goat anti Mouse IgG (H&L) antibody (Thermofisher scientific, #A-11001) or AF-488 Goat anti Mouse IgG1 (Thermofisher scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibody incubations were performed for 2hr at room temperature with Alexa Fluor 594-conjugated anti-rabbit IgG and Alexa Fluor 488-conjugated anti-mouse IgG (1:100; H+L, Molecular Probes, Eugene, OR). To detect DNA 1ng/µl 4′6′-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2024Quote: ... And these secondary antibodies were used: Alexa Fluor 568 Goat Anti-Mouse IgG H&L (1:1000; Thermo Fisher Scientific, A11031, RRID: AB_144696), Alexa Fluor 700 Goat Anti-Rabbit IgG H&L (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... and purified PCR fragments were inserted into the vector pENTR/D-TOPO using a pENTR Directional TOPO Cloning Kit (Life Technologies). The resulting entry clones were confirmed by sequencing ...
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bioRxiv - Cell Biology 2020Quote: Cells were grown in 12 well tissue culture plate coated with Poly-D Lysine and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher Scientific, Walktham, MA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with FACS buffer and incubated with the live-dead marker 7-aminoactinomycin D (7-AAD; Thermo Fisher Scientific) for 10 min at 4 °C in the dark ...
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bioRxiv - Developmental Biology 2021Quote: The two-cell embryos from IVF were washed and placed in a dish of equilibrated KSOM media (Cat. No. MR-106-D, Fisher Scientific) covered by light mineral oil (Cat ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked with 5% BSA in TBST buffer and probed with anti-mouse Tim-3 goat polyclonal antibody (R&D) and detected with anti-goat-HRP antibody and SuperSignal West Pico Chemiluminescent reagent (Thermo Fisher).
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bioRxiv - Physiology 2022Quote: ... entry cDNA was PCR amplified from Atg8 cDNA (clone LD05816 DGRC-Gold collection) and cloned into pENTR-D/TOPO using BP reaction (Gateway® BP Clonase II enzyme mix, Cat#11789-020, Invitrogen). For Drosophila Upd2 variants ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were grown in (D)MEM supplemented with 10% fetal bovine serum (FBS)/25mM HEPES/GlutaMAX™/ Penicillin-/Streptomycin (Gibco) and maintained at 37ºC in a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: TeloHAECs were transfected with a pool of Atp6ap2 (Dharmacon, L-013647-01-0005) and non-targeting control-siRNAs (Dharmacon, D-001810-10-05) using Lipofectamine 3000 (ThermoFisher, L3000015) following manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: ... neural precursor cells were thawed and resuspended in a chemically defined medium and added to sterile-culture plates coated with poly-D-lysine and laminin (ThermoFisher Scientific). The medium was replaced 24 hr after plating ...
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bioRxiv - Genetics 2021Quote: ... Differentiation was performed on sterile culture plates coated with poly-D-lysine (10 µg/ml) and laminin [10 µg/ml (ThermoFisher Scientific) in differentiation medium (Millipore Sigma ...