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Citations for Thermo Fisher :
6201 - 6250 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein expression was induced by the addition of 50 μM Isopropyl β-D-1-thiogalactopyranoside (Fisher Scientific, Fair Lawn, NJ USA) to bacterial cell cultures for 2.5 hours with shaking at 37°C ...
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bioRxiv - Microbiology 2021Quote: Tagged diguanylate cyclases were generated by PCR amplification of each diguanylate cyclase followed by cloning into pENTR D-TOPO (ThermoFisher Scientific) and then transferred to either pRH016 (HA tag ...
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2021Quote: ... OCR and ECAR were measured in un-buffered DMEM supplied with 4.5g/L D-glucose and 10mM L-glutamine (Gibco, MA, USA). OCR and ECAR values were normalized to cell numbers ...
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bioRxiv - Immunology 2020Quote: ... D-PBS (D-PBS without Ca++ and Mg++) supplemented with 2% FBS and 3 mM cell culture grade EDTA (Life Technologies; Thermofisher) prior to monocyte isolation ...
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bioRxiv - Genomics 2021Quote: ... the tissue was sectioned and up to 8 sections of 10 µm sections were placed in Lysing Matrix D tubes (MPBiomedicals, cat.no.: 116913050-CF) containing Buffer RLT Plus (Quiagen, cat.no.: 1053393) and ß-Mercaptoethanol (Thermofisher, cat.no.: 31350010) and homogenized in a Fastprep instrument (ThermoSavant) ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 [1-301] fragment N-terminally fused to a 6xHis tag was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Microbiology 2022Quote: ... Synthetic cDNA encoding a self-cleaving polyprotein Bcl-6.t2A.Blc-xL to express the germinal center B cell-associated transcription factors Bcl-6 and Bcl-xL was synthesized (B6L; Integrated DNA Technologies) and cloned in pENTR/D-TOPO (Thermo Fisher), generating pENTR.B6L (35 ...
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bioRxiv - Physiology 2023Quote: Cell lines expressing mV:CAHS D and mV:2X Linker variants were seeded and stained with 5 µg/mL Hoechst 33342 dye (Catalog 62249, Thermo Fisher) for nuclear staining and 1x Cell Brite Red (Catalog 30108-T ...
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bioRxiv - Cancer Biology 2023Quote: ... and siRNA non-targeting control siRNA pools (Dharmacon D-001810-10-05) were mixed with Lipofectamine RNAiMax (5 µL, ThermoFisher 13778075) in a total of 200 µL Optimem (Life Technologies 31985-062 ...
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bioRxiv - Neuroscience 2023Quote: ... The mixture of nuclei and beads in each sample was placed on a Dyna-2 magnetic stand (#12321.D, Thermofisher Scientific) for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of purified RNA was retro-transcribed with oligos d(T)18 by using SuperScript IV First-strand System (Invitrogen, #18091050). For RT-qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... or non-targeting control siRNA pools (ON-TARGET plus non-targeting pool D-001810-10-05) using Lipofectamine RNAiMAX transfection reagent (Life Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... To this end the coding sequence of bmp7b (ZFIN-ID: ZDB-GENE-060929-328) was amplified from cDNA and cloned into pENTR/D-TOPO (Thermo Fisher). The transgenesis construct was generated by Multiway Gateway (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... the full-length MpNEK1 coding sequence in the pENTR/D-TOPO entry vector (Otani et al. 2018) was transferred by LR reaction (LR clonase [ enzyme mix, Life Technologies) into the Gateway binary vector pMpGWB344 (MpEF1α pro:XVE >>LexA operator:Gateway cassette).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected with the TRIB1 targeting (L-003633-00-0010) or non-targeting control (D- 001810-10-2) ON-TARGETplus siRNA SMARTpools (Dharmacon) using RNAiMAX (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... of MIR319C were amplified from pMIR319C-P4P1R entry clone using Taq DNA polymerase and cloned into pENTR/D-TOPO vector (K240020, Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 1µg of RNA samples were used as template in cDNA generation using Oligo d(t) primers and Superscript IV (Invitrogen 18091050) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... neurons were plated at a density of 0.8x106 cells per well of 6-well plates coated with 50 ug/mL Poly-D-Lysine (Thermofisher, #A38904-01) and 1ug/mL Laminin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... hippocampi were triturated and immediately plated in DMEM containing 10% fetal bovine serum on poly-D-lysine (Thermofisher; catalog no. ICN10269491) coated 18 mm glass coverslips (Warner instruments ...
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bioRxiv - Plant Biology 2022Quote: ... which were a gift from Kazuya Ishikawa and cloned into the pENTR vector using the pENTR/D-TOPO Cloning Kit (Thermo Fisher). These pENTR constructs were used as templates for site-directed mutagenesis to introduce SYT5 S66F or SYT7 G427R mutations (primers in Table S1 ...
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bioRxiv - Cell Biology 2022Quote: HSC cells atop ECM hydrogels subjected to the different treatments for 3 d were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at room temperature for 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... coding sequence were PCR-amplified from maize leaf cDNA with the appropriate primers (Supplementary Table 1) and cloned into the pENTR/D-TOPO entry vector (Thermo Scientific). Clones were recombined with the vectors pSITE-2CA and pSITE- 1NB using LR Clonase Mix II (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed once with Dulbecco’s Phosphate Buffered Saline (D-PBS, Nacalai Tesque) and incubated in TrypLE Express (Thermo Fisher Scientific) for 10 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... 000 cells were seeded onto a 6-well plate and reversely transfected with 25 nM of either non-targeting control siRNA (Horizon Discovery, D-001206-13-20) or SMARTpool siRNA targeting Znf706 (Horizon Discovery, M-021025-01-0005) using Lipofectamine RNAiMAX (Invitrogen, 13778150). The transfection media was exchanged after 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... Organs were homogenized using a FastPrep tissue homogenizer (MP Bio) in Lysing Matrix D tubes (MP Bio) containing Tissue Protein Extraction buffer (T-PER, Thermo Scientific) supplemented with protease inhibitor tablets (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... the MpROPGAP and MpREN coding sequences were amplified using PCR by KOD Plus Neo DNA polymerase (TOYOBO) with the appropriate primer pairs (Table S1) and cloned into the pENTR/D-TOPO cloning vector (Life Technologies). To generate proMpE2F:XVE>>MpROPCA ...
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bioRxiv - Plant Biology 2024Quote: ... were amplified from wild-type Tak-1 genomic DNA using PCR with the appropriate primer pairs (Table S1) and cloned into the pENTR/D-TOPO cloning vector (Life Technologies). These entry vectors were used in the Gateway LR reaction (Life Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... using 200 psi and µm tungsten particles coated with fluorescent 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI, Thermo Fisher Scientific; D-282). The DiI was allowed to diffuse at r.t ...
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bioRxiv - Neuroscience 2023Quote: ... that had been coated with poly-D-lysine and laminin and cultured in F12 medium with 5% fetal calf serum and penicillin-streptomycin (Thermo Fisher). Neurons were cultured for 2-5 days before fixation with 4% paraformaldehyde in a cytoskeletal-stabilizing buffer 25 at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2024Quote: Cultured HEK293 cells were grown on 12 mm round cover glasses (Azer Scientific, #200121) pre-coated with poly-D-lysine (Gibco, #A3890401) in 24-well plates and then transfected with a plasmid encoding HA-tagged neurexin1 alpha using lipofectamine 2000 ...
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bioRxiv - Neuroscience 2024Quote: ... VT027328 primers127 were used to amplify the sequence from the genomic DNA of VT027328-p65AD fly line (BDRC #73064) The verified sequence was then assembled into the pENTR/D-TOPO vector (Thermo Fisher) and integrated into the pBPp65ADZpUw vector using the Gateway LR Clonase II Enzyme mix (Thermo Fisher).
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bioRxiv - Genomics 2024Quote: ... L-008436-00) or negative control siRNA (ON-TARGETplus Non-targeting Control Pool [Horizon Discovery], D-001810-10) using Lipofectamine RNAiMAX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... L-008436-00) or negative control siRNA (ON-TARGETplus Non-targeting Control Pool [Horizon Discovery], D-001810-10) using Lipofectamine RNAiMAX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... along with a scrambled siRNA (DharmaconTM, D-001810-01) as a negative control at 30 nM final concentration using Lipofectamine RNAiMAX reagent (Invitrogen, 13778150), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... on non-tissue-culture treated 6-well plates that were pre-treated overnight with PBS + 0.1 mg/ml Poly-D-lysine (Thermo Scientific, A3890401) and 200 μg/ml human JAGGED1-Fc ...
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bioRxiv - Plant Biology 2023Quote: ... and for promoter::GUS/GFP reporter fusion construct (proJAZ1::GUS/GFP – 667 bp) sequences of the genes were amplified and was first cloned into pENTR/d-TOPO (Invitrogen, Life Technologies). For over-expression under CaMV 35S promoter (35S::ABI3 ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... or a non-targeting control siRNA (siGENOME Non-Targeting siRNA 5, Horizon Discovery, # D-001210-05-05) and 5 µl Lipofectamine RNAiMAX (Invitrogen, #13778075) in a total volume of 2 ml in antibiotics free 10% DMEM ...
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bioRxiv - Physiology 2024Quote: ... digests and PBs were filtered through glass fiber membrane (Whatman Grade GF/D, 2.7 µm pore size, Cytivia, Thermo Fisher, Nepean), using a glass vacuum filtration apparatus ...
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bioRxiv - Neuroscience 2023Quote: ... into low passage HEK-293T cells plated at 1×106 cells/well of a poly-D-lysine-coated 6-well plate using Lipofectamine 3000 (Life Technologies). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... cell slides were put into a 12-well plate and pre-treated with 0.1 mg/ml poly-D-lysine (ThermoFisher Scientific, A3890401). The chemokine receptor stable expressing HEK293T cells were then seeded into the slides and cultured overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed prior to splitting each sample into two aliquots for D-2-HG quantification and total protein quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 44-2404-21) were acquired from Nalge-Nunc International (Rochester, NY, USA). D-Luciferin (Cat. No. 88293) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biophysics 2024Quote: ... Cells were then washed three times with D-PBS (+/+) followed by incubation with Alexa Fluor™ 555 anti-mouse secondary antibody (#A-21422, Invitrogen) at a 1:1000 dilution for 1h at RT ...
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bioRxiv - Immunology 2024Quote: ... washed and 1×105 cells in 50 µL of ATP assay media were plated per well on a Poly-D-lysine (Gibco, A3890401) coated XF96 microplate (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... gene was amplified from cDNA by Phusion PCR using specific primers (Table S7) and subsequently cloned into the pENTR/D-TOPO vector (Invitrogen, K240020) to generate the pME-spns1 vector ...
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bioRxiv - Cancer Biology 2022Quote: ... p41Plk3 mammalian expression vector for protein expression and purification was generated by performing an LR recombination reaction between the pENTR/D-TOPO entry clone and a Gateway destination vector pcDNA-DEST40 containing His-tag (Life Technologies). ShPlk3s (shRNA-A ...
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bioRxiv - Cancer Biology 2022Quote: ... Inducible full-length Plk3 and p41Plk3 were constructed by subcloning the corresponding coding regions of Plk3 into a pENTR/D-TOPO entry vector for Gateway cloning (Life Technologies) followed by an LR recombination reaction using Gateway LR Clonase II enzyme mix (Life Technologies ...