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Citations for Thermo Fisher :
5951 - 6000 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... fixed and permeabilized after 16 h and stained with Alexa Fluor AF® 594 phalloidin (0.1 U/ml, Molecular Probes, Thermo Fisher Scientific) to reveal filamentous (f)-actin ...
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bioRxiv - Systems Biology 2022Quote: ... The synthesized product was digested by incubation for 1 h at 37 °C with 1 ul of FastDigest BamHI (ThermoFisher cat. no. FD0054) and 1 ul of FastDigest SbfI (ThermoFisher cat ...
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bioRxiv - Microbiology 2022Quote: ... and goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody (Alexa Fluor® 488; 1:500 dilution (Invitrogen, cat# A-11029)) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed and incubated with 1 µg/ml AlexaFluor647 Donkey Anti-Mouse IgG (H+L) (Thermo Fisher Scientific Corporation, OR, USA) at room temperature for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed 5 x 5 mins and 1 x 1hr before corresponding secondary antibodies along with the nuclear stain Hoechst33342 (1:5000; Molecular Probes; H-3570) were incubated overnight at 4°C ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... cells transfected with D4H-mCherry were incubated for 16 h in growth medium containing 70 μg/ml 10kDa dextran conjugated with Alexa Fluor 647 (Thermo Fisher Scientific, D22914). Growth medium was replaced with Opti-MEM 2 h prior to imaging ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L) HRP conjugates (Thermo Fisher Scientific; #31430 or #32460).
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were incubated for 3 h at room temperature in Alexa Fluor 405-conjugated streptavidin (ThermoFisher Scientific, Cat# S32351, 6.25 μg/ml). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... Adjacent sections were used for fluorescence photomicrography and staining for nuclei (Hoechst, Cat No. 33342, Thermo Fisher, 1:1000; DAPI, H-1200, Vectashield). Photomicrography was conducted using a fluorescence microscope (Eclipse 80i ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 488 Donkey anti-Rabbit IgG (H+L, Catalog # A-21206), Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L, Catalog # A-31573) were purchased from Invitrogen (Manufacturer: Life Technologies). For the WT mice used for restraint stress ...
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bioRxiv - Immunology 2020Quote: ... The slices were blocked with 1% BSA/PBS for 1 h and stained with anti-Krt5 (Invitrogen cat. MA5-17057, 1:100 dilution), anti-PPP6C (Abcam cat ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were washed with PBS buffer three times and incubated in secondary antibody solution [1:1000 Alexa Fluor™ 546 goat anti-mouse IgG (H+L) (Thermo Fisher Scientific) for use in experiments with anti-HA and anti-catalase antibodies as the primary antibodies and 1:1000 Alexa Fluor™ 488 goat anti-mouse IgG (H+L ...
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bioRxiv - Plant Biology 2021Quote: ... for use in experiments with anti-HA and anti-catalase antibodies as the primary antibodies and 1:1000 Alexa Fluor™ 488 goat anti-mouse IgG (H+L) (Thermo Fisher Scientific) for use in experiments with the anti-α-tubulin antibody as the primary antibody ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were incubated for 1 h at RT with appropriate secondary antibodies conjugated to Alexa Fluor 488 or 594 (Molecular Probes, ThermoFisher 1:500) diluted in 0.1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody (200 μL) was added (1:1,000 dilution of Alexa Fluor™ goat anti-mouse 594 IgG (H+L) (Invitrogen, A11005, Lot 1937185) in 1x PBS ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Biophysics 2022Quote: ... We revealed the antigens coating with a Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 647, Invitrogen, ref. A-21447) and analyzed the fluorescence intensity ...
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bioRxiv - Developmental Biology 2022Quote: ... The secondary antibodies used for immunofluorescent staining were Alexa Fluor 488/594-conjugated goat anti-rabbit IgG (H+L) (A-11008/A-11012, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse IgG H&L (Alexa Fluor 488/594 ...
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bioRxiv - Genetics 2020Quote: ... 0.1% formic acid at a flow rate of 200 nL/min followed by acetonitrile wash and column re-equilibration for a total gradient duration of 4 h with a RSLC Ultimate 3000 (Thermo Fisher Scientific, Dionex). Peptides were sprayed using an EASYSpray source (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated for 1 h with an F(ab’)2-Goat anti-Mouse IgG (H+L) cross-adsorbed secondary antibody conjugated with Alexa Fluor 488 (Invitrogen, Thermo Fisher Scientific) diluted in blocking buffer ...
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bioRxiv - Systems Biology 2021Quote: ... 0.1 % formic acid at a flow rate of 200 nL/min followed by acetonitrile wash and column re-equilibration for a total gradient duration of 4 h with a RSLC Ultimate 3000 (Thermo Fisher Scientific, Dionex). Peptides were sprayed using an EASYSpray source (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed Bond Wash solution for 3 × 3 minutes and then incubated with a goat anti-mouse IgG (H+L) AlexaFluor 488-conjugated secondary antibody (A-1101, ThermoFisher Scientific, Waltham, MA) 1:500 in PBS containing 3% goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed with Bond Wash solution 3× 3 minutes then incubated with a goat anti-rabbit IgG (H+L) AlexaFluor 633-conjugated secondary antibody (A-21070, ThermoFisher Scientific, Waltham, MA) 1:500 in PBS containing 3% goat serum ...
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bioRxiv - Biochemistry 2020Quote: ... Urine metabolite separation and analysis was conducted using a Waters ACQUITY H-class LC system coupled with a LTQ-Orbitrap Velos Pro mass spectrometer (Thermo Fisher Scientific, MA). The following 18-min gradient on a Waters HSS C18 column (3.0 × 100 mm ...
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bioRxiv - Biochemistry 2020Quote: ... and then incubated for 1 h in the blocking solution containing 0.1 % Tween-20 and AlexaFluor® 680 goat anti-rabbit (H+L) secondary antibody (1: 20, 000; ThermoFisher Scientific, Waltham, Massachusetts) (Table S5) ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 1 ×104 cells/well were cultured for 36 h in DMEM supplemented with 10% dialyzed FBS (Gibco, Thermo Fisher Scientific). Lactate was measured in the conditioned media using the Lactate-Glo Assay (Promega ...
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bioRxiv - Physiology 2021Quote: HeLa cells and PC12 cells were seeded in 48-well plates at a density of 1×105 cells/well in 250 µL of RPMI 1640 medium (10% HS, 5% SFB and Ab-Am) grown for 24 h and transfected with Lipofectamine 2000 (Thermo Fisher, MA, USA) with 0.8 µg of the reporter plasmid (Gal4-dependent MH100tk-Luc) ...
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bioRxiv - Biophysics 2020Quote: ... in 10 mM PBS to a total reaction volume of 200 μL and incubating at 37° C for 18 h with 250 rpm shaking inside an incubator (Thermo Fisher Scientific, MaxQ600). Preparation of crosslinked fibrin involved two additional reagents ...
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bioRxiv - Immunology 2021Quote: ... Following air drying to evaporate residual acetone the fixed slides were blocked for 1 h at room temperature in 10% normal goat serum (Invitrogen, Thermo Fisher Scientific, 10000C) in phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... monocytes were allowed to adhere to culture plates (Costar) for 1 h at 37°C in warm RPMI-1640 medium (ThermoFisher Scientific, Waltham, MA). The cells were then washed with warm phosphate buffered saline (PBS ...
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bioRxiv - Genetics 2020Quote: ... Protoplasts were collected 48 h after transformation and DNA was isolated with PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, Catalog number: K182002) following the manufacture’s protocol.
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Sections were then washed three times in PBS before being incubated for 1 h at room temperature with an Alexa Fluor® 594 goat anti-rabbit IgG antibody (A-11037, Invitrogen, Waltham, MA). Sections were then washed three times in PBS and slides were cover slipped using Prolong™ Gold (P36930 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA synthesis was performed with 0.5-5 μg of total RNA using the Maxima H minus Reverse Transcriptase kit (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2021Quote: Total RNA of cells was extracted after treatment of cells with sertraline hydrochloride for 72 h using TRIzol reagent (Cat no. 15596-018, Ambion, Life Technologies, USA). Complementary DNA (cDNA ...
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bioRxiv - Developmental Biology 2021Quote: Full-length cDNA synthesis from RNA was performed using Maxima H Minus reverse transcriptase (K1651, Thermo Scientific, Fisher Scientific, Hanover Park, IL, USA) following the manufacturer’s directions with 2 µg of total RNA and 100 pmol of d(T)20 VN primer ...
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bioRxiv - Cancer Biology 2021Quote: ... 2012) (a gift from Andrew Jackson) at 1:300 and rabbit anti sheep IgG (H+L) secondary link antibody (Invitrogen #31240 1:4000) following Leica BondRx standard IHC protocols ...
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bioRxiv - Biochemistry 2021Quote: ... Cell culture supernatant was harvest 42 h post-infection and viral RNA was purified using MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher Scientific). The samples were processed using the semi- automated NucliSENS® easyMag® platform (bioMérieux ...
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bioRxiv - Neuroscience 2021Quote: ... tissues were washed with PBS and were incubated with the following secondary antibodies for 1 h at room temperature: donkey anti-goat Alexa Fluor 488 (1:100; Invitrogen, A32814, RRID: AB_2762838), donkey anti-chicken Alexa Fluor 488 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... The filtrate was enzymatically digested for 1 h with 500 μg ml-1 Collagenase type II (Cat no.: 17101-015, Gibco, Grand Island, NY)) ...
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bioRxiv - Cancer Biology 2021Quote: Full-length human SETD7 cDNA sequences were amplified from a human mRNA pool generated by RT-PCR using SuperScript II RNase H Reverse Transcriptase (Life Technologies,CA, USA). The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... the microplates were incubated at 37°C and 400 rpm for 24 hours (h) in an incubating microplate shaker (Fisher Scientific, PA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... antibodies for 12 h at 4 °C followed by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1/500; Invitrogen, Thermo Fisher Scientific, Inc.). After a PBS wash ...
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A Novel Mice Model of Catecholaminergic Polymorphic Ventricular Tachycardia Generated by CRISPR/Cas9bioRxiv - Molecular Biology 2021Quote: ... Freshly isolated cardiomyocytes were plated on 25-mm cover-slips pre-coated with 40 μg/ml laminin for 1 h (Thermo Fisher, MA, USA). All cardiomyocytes were cultured in M199 medium (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:3000 (PBS milk 1% -Tween 0.1%) for 1 h at 25°C and detected using 1-Step(tm) Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Waltham, MA). Plates were read using Cytation5 (BioTek ...
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bioRxiv - Immunology 2020Quote: ... Alexa Fluor 488 goat anti-rabbit IgG (H+L) and Alexa Fluor 488 goat anti-mouse IgG1 (g1) were purchased from Fisher Scientific (Waltham, MA).
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bioRxiv - Immunology 2021Quote: ... Cells were stained for surface markers then fixed and permeabilized for at least 16 h using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific). Cells were then stained with directly labeled Abs to NOS2 (CXNFT ...
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bioRxiv - Microbiology 2020Quote: The plasmids containing H- or L-chain genes (50 µg each) were transduced to 1 × 108 293F cells using 293fectin reagent (ThermoFisher Scientific, Waltham, MA). After incubating the cells at 37°C for 4 days ...
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bioRxiv - Developmental Biology 2022Quote: ... Electrophoresis was conducted at 150 V for 4 h on ice in NuPAGE™ MES SDS Running Buffer (20X) (Thermo Fisher Scientific, NP0002) supplied with 1x NuPAGE™ Antioxidant (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... diluted 1:100 in blocking solution was followed by a 1-hour incubation with Alexa Fluor 568 Donkey anti-Sheep IgG (H+L) secondary antibody (Invitrogen, A-21099, RRID:AB_2535753) diluted 1:400 in blocking solution ...