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Citations for Thermo Fisher :
5901 - 5950 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... MIR221 Silencer® Select (#4390771) and negative control (D-001810-10-05) ON-TARGETplus SMARTpool siRNAs were purchased from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Tumor-bearing liver tissue was minced and incubated in collagenase Type IV solution (1x D-MEM with 400 U/mL Type IV collagenase powder (Gibco) and 0.5 μg/mL Amphotericin B solution (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... NPCs were differentiated into astrocytes within 7 days by changing the medium to D-MEM (high glucose) (Fujifilm Wako, Tokyo, Japan) supplemented with 10% FBS (A5256701, ThermoFisher) and 1% Penicillin-Streptomycin at 24 h in vitro.
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bioRxiv - Developmental Biology 2024Quote: ... 2.5 mM CaCl2, 1.2 mM MgSO4, 1.2 mM KH2PO4, 25 mM NaHCO3, and 11 mM D-glucose, all from Thermo Fisher Scientific). Vessels were allowed to equilibrate in the chambers for at least 45 min after normalization ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the appropriate secondary antibody (polyclonal goat anti-mouse IgG1, 1:500, ThermoFisher A21124; polyclonal goat anti-rabbit IgG H&L, 1:500, ThermoFisher A11011, Waltham, USA) diluted in blocking solution for 45 min at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cellular debris was removed by centrifugation at 5,488x g for 40 min at 4°C (Heraeus™ Megafuge™ 40R) and incubated for 2 h with 500 μl HisPurTM Cobalt Resin (89964, ThermoFisher Scientific) at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... They were then incubated with primary antibodies for 3 h at room temperature and with secondary antibodies in accordance with the manufacturer’s protocol (Life Technologies/Thermo Fisher Scientific). For cell surface MMP14 staining ...
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bioRxiv - Cancer Biology 2021Quote: ... Triton X-100 and 40 mg/mL BSA for 30 min and incubated with primary antibodies for 3 h at room temperature and secondary antibodies in accordance with the manufacturer’s protocol (Life Technologies/Thermo Fisher Scientific). The samples were viewed and analyzed under an LSM880 laser scanning microscope using a 20x air objective (Carl Zeiss) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed twice with the PBSA buffer and incubated with the Alexa Fluor® 488 Donkey Anti-rabbit IgG (H+L) Antibody (Thermo Fisher Scientific) at 1:500 dilution for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with secondary antibody anti-mouse IgG (H+L) coupled to horseradish peroxidase (1/20000; ThermoFisher Scientific Cat# 32230, RRID:AB_1965958). The immunoblot was revealed with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher Scientific Cat# 34580 ...
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bioRxiv - Cell Biology 2020Quote: ... were plated in six-well plates and transfected with CD44 siRNA for 24 h in antibiotic-free media using siRNA Lipofectamine RNAiMAX and OPTI-MEM I reduced serum medium (Invitrogen, Carlsbad, CA, USA), in accordance with the siRNA manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of total RNA (1 μg) were reverse transcribed using the Maxima™ H Minus cDNA Synthesis Master Mix (M1661, Thermo Fisher Scientific). To analyze gene expression ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated for 1 h with a secondary antibody (goat anti-rabbit IgG, Alexa Flour 488 conjugate, ThermoFisher Scientific/Invitrogen, R37116) and DRAQ5 (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... with mutated Zld and Su(H) binding sites in the 5kb dpn cis-regulatory and used Gateway LR Clonase (ThermoFisher Scientific, Waltham, MA) to clone the region into the VanGlow vector without the DSCP (Addgene#83342 ...
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bioRxiv - Bioengineering 2022Quote: ... The sample were then incubated with 1:500 Alexa Fluor 488-conjugated goat anti-Mouse IgG (H+L) secondary antibody (Thermo Fisher Scientific, A11001) in blocking buffer at RT for 45 min ...
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bioRxiv - Systems Biology 2022Quote: ... The synthesized product was digested by incubation for 1 h at 37 °C with 1 ul of FastDigest FspAI (ThermoFisher cat. no. FD0054) and 1 ul of FastDigest PmeI (ThermoFisher cat ...
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bioRxiv - Neuroscience 2021Quote: ... mAb24B10 (DSHB, 1:50), anti-OLLAS (Funakoshi, 1:200) and goat anti-rat IgG(H+L) Alexa Flour 488 conjugate (Life Technologies, 1:400).
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies were used in this study were: goat anti-rabbit IgG (H&L) conjugated with DyLight 488 (Thermo Fisher Scientific, 35553) and goat anti-mouse IgG (H&L ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were washed three times with PBS containing 0.2% Triton X-100 and incubated for 1 h at room temperature with anti-mouse secondary antibody conjugated with Alexa 488 (Thermo Fisher Scientific, R37120) diluted 1:1000 with Intercept (TBS ...
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bioRxiv - Immunology 2021Quote: ... Slides were washed and then incubated for 1 h with Alexa Fluor 647 donkey goat anti-rabbit IgG (Life technologies #A21244, 1:500). NucBlue™ Live Cell Stain (Thermo Fisher #R37605 ...
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bioRxiv - Immunology 2021Quote: ... then permeabilized and stained with antibodies for intracellular protein for 1 h using the Foxp3 Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA). Data were collected in a FACSCalibur flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Transduced cells were selected by culturing the cells in the presence of 2 μg/mL puromycin (replenished every 48 h) (Gibco; Cat no A1113802) for 7-14 days ...
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bioRxiv - Pathology 2020Quote: ... sections were incubated for 1 h with secondary antibody (Alexa Fluor® 488 goat anti-rabbit IgG (ThermoFisher Scientific, A-11008, 1:200), or Alexa Fluor® 594 goat anti-rabbit IgG (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 1 h with secondary antibody labeled with Alexa 488-labeled goat anti-rabbit IgG (1:200; Molecular Probes, Eugene, OR) at 25°C in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were also diluted in permeabilization buffer and incubated for 1 h at room temperature: donkey-anti-rabbit Alexa Fluor 488 and donkey-anti-mouse Alexa Fluor 647 (both ThermoFisher, 5 μg/mL). Cells were finally washed with 0.1% Triton X-100 in PBS to remove any background staining and mounted usin mounting medium with 0.01% Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid ...
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bioRxiv - Biochemistry 2020Quote: ... Data Evaluation: Peaks corresponding to the calculated amino acid masses (MIM +/− H+ ± 2 mMU) were integrated using TraceFinder software (Thermo Scientific, Bremen, Germany). Alternatively ...
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bioRxiv - Bioengineering 2020Quote: ... the gel was washed three times for 10 min in water followed by a 1 h staining period with PageBlue® Protein Staining Solution (Thermo Fisher Scientific). For MS analysis gel bands corresponding to high molecular weight range proteins were excised ...
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bioRxiv - Bioengineering 2021Quote: MOLM-13 cell clones cultured on-chip for 72 h were stained for the hematopoietic marker CD45 using a PE-conjugated primary antibody (mouse anti-human CD45-PE, Invitrogen #12-0451-82). Prior to imaging ...
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bioRxiv - Plant Biology 2021Quote: ... Seedlings were imaged 72 h after Dex induction and after 15 min of staining with 1 µM FM4-64 (T13320, ThermoFisher Scientific, MA, USA) in liquid medium (0.5 x MS medium ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA (3.6 μg) was used as template for reverse transcription with RevertAid H Minus Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA) and primed with an oligo(dT ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 0.5 mL of PBS and incubated for 1 h at room temperature under vigorous shaking with 50 μL of Qdot™ 705 Streptavidin Conjugate (SA-QD705, Thermo Fisher Scientific) prepared at 0.1-10 nM in PBS buffer + 6% BSA ...
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bioRxiv - Neuroscience 2020Quote: A custom gene array for inflammasome genes was used following stimulation of iPS-Mg with PS+ cells or LPS for 24 h (TaqMan™ Array Plate 16 plus Candidate Endogenous Control Genes; Thermo Fisher Scientific). Complementary DNA was generated from iPS-Mg RNA samples using the High-Capacity RNA-cDNA kit (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... The cells were again washed three times with PBS and then stained with an Alexa Fluor 594-conjugated goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody (Thermo Fisher Scientific #A11037), diluted in BSA/PBS for 1 h in the dark at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor 488 goat anti-rabbit IgG (H+L) and Alexa Fluor 488 goat anti-mouse IgG1 (γ1) were purchased from Fisher Scientific (Waltham, MA).
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bioRxiv - Neuroscience 2020Quote: ... and then incubated at room temperature for 4-5 h in Triton PBS containing Alexa Fluor 594-conjugated donkey anti-rabbit IgG (1:1000; A-21207, Thermo Fisher Scientific, RRID:AB_141637). After binding of streptavidin/antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed three times with PBST and stained with donkey anti-rabbit IgG (H+L) Alexa Fluor Plus 488 (Thermo Fisher Scientific, # A32790) in blocking buffer for 1 hour at room temperature followed by 1 hour of DAPI (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... Following 2 h incubation at room temperature with an anti-rabbit peroxidase-conjugated secondary antibody (Dilution: 1:10000, Thermo Fisher Scientific, Ref: 31460) diluted in PBS pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... Cultures were maintained at 37°C in 5% CO2 for 2 h before the plating medium was replaced with Neurobasal growth medium containing B27 supplements (Life Technologies Inc., USA). The cells were used for experiments after 6 days in culture ...
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with AlexaFluor647 donkey anti-sheep IgG (H+L) (Thermo Fisher, UK, catalogue # A-21448); or 5 μg/mL SARS-CoV-2 S test protein (Peak Proteins ...
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bioRxiv - Cell Biology 2021Quote: ... 3 x 1 h) and incubated with the secondary antibody (Alexa-labelled goat anti-mouse 555 plus, A32727, Thermo Fisher Scientific, 1:1000), an anti-GFP antibody conjugated with Alexa 488 (A-21311 ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were washed 3 times in PBV before incubation for 2 h at room temperature in the dark with anti-rabbit Alexa Fluor 594-conjugated secondary antibody (Life Technologies, Carlsbad, CA) at 1:500 dilution with 10 % goat serum in PBV ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were then incubated for 2 h at room temperature with an appropriate secondary antibody consisting of goat anti-mouse Alexa Fluor 488 (A11029, Fisher Scientific, Pittsburgh, USA) diluted 1:500 and wheat germ agglutinin Alexa Fluor™ 350 Conjugate (W11263 ...
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bioRxiv - Neuroscience 2022Quote: ... the secondary antibody used in staining was donkey anti-goat IgG (H+L) highly cross-adsorbed secondary antibody conjugated to Alexa Fluor Plus 488 (ThermoFisher Scientific, A11055;1:500). The RNAscope probes used in our experiments were ...
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bioRxiv - Systems Biology 2022Quote: ... and the resulting supernatant (100 ng/ml) was incubated for 4 h at 37°C together with 5 mM glutamine (Gibco, Cat# 25030-081), 1 mM phosphoribosyl pyrophosphate (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were incubated with secondary antibodies for 1 h at room temperature and counterstained by DAPI (1:1000, Thermo Scientific™, Catalog #:62248), coverslipped with EverBrite mounting medium (Biotium ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Membranes were subsequently probed with goat anti-rabbit IgG (H+L) conjugated to HRP according to manufacturer’s instructions (Thermo Fisher, 1:1,000 dilution). Bands were detected with SuperSignal West Pico Plus substrate (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2022Quote: ... For functional characterization three fresh colonies of each biosensor were picked and inoculated into 0.5 mL of LB with corresponding antibiotics and grown at 37°C for 16 h in 96 DeepWell polystyrene plates (Thermo Fisher Scientific, 278606) sealed with AeraSeal film (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The whole retinas were incubated at RT for 1 h with Lectin PNA conjugated to Alexa Fluor 647 (1:50, L32460, Life Technologies, Carlsbad, CA) in blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was subsequently incubated with the goat anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody conjugated to the fluorophore Alexa Fluor plus 555 (diluted 1:5000, Thermo Fisher Scientific, USA) before imaging ...
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bioRxiv - Bioengineering 2022Quote: ... fixed and permeabilized after 16 h and stained with Alexa Fluor AF® 594 phalloidin (0.1 U/ml, Molecular Probes, Thermo Fisher Scientific) to reveal filamentous (f)-actin ...