Labshake search
Citations for Thermo Fisher :
6251 - 6300 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred to nitrocellulose membrane (Invitrogen) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
-
bioRxiv - Biophysics 2020Quote: ... authenticated using a short tandem repeat assay (University of Arizona Genetics Core) and cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 (Gibco, 11320-074) supplemented with 8% fetal bovine serum (Sigma ...
-
bioRxiv - Biophysics 2020Quote: ... CHO-K1-A1R and CHO-K1 cells were routinely cultured in Hams F-12 nutrient mix (21765029, Thermo Fisher Scientific) supplemented with 10% Foetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... with 10% FBS and 1% pen/strep and MCF10A cells were cultured in DMEM/F-12 supplemented with 5% horse serum (Gibco), 20ng/ml epidermal growth factor ...
-
bioRxiv - Genomics 2020Quote: ... The sample was run on ice at 150V in 1x MOPS Buffer for 75 minutes on a Novex NuPAGE 4-12% Bis-Tris Gel (NP0321, Invitrogen) with 10μL dilute protein ladder (12μL Wash Buffer ...
-
bioRxiv - Microbiology 2020Quote: Samples were suspended in diluted with Laemmli sample buffer and typically electrophoresed on a 4–12% gradient SDS-polyacrylamide gel (NuPAGE Novex, Invitrogen). Gels were either stained with Coomassie blue or transferred to a nitrocellulose membrane using an iBlot system (Invitrogen) ...
-
bioRxiv - Genetics 2021Quote: ... Aliquots equivalent to 1 μg and 3 µg protein were separated on NuAGE 12% Bis-Tris Gels using MOPS 1X (Invitrogen) running buffer ...
-
bioRxiv - Immunology 2020Quote: ... Equal amounts (∼20 μg) of protein extracts were separated by electrophoresis on 4-12% NuPAGE Bis-Tris Mini Gels (Invitrogen) and then transferred to nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Microbiology 2020Quote: ... samples were heated (70°C) in Laemmli reducing sample buffer before separation on 4-12% Tris/Bis gradient SDS-PAGE gels (Invitrogen). After electrophoresis ...
-
bioRxiv - Immunology 2021Quote: RNA was isolated from 12 fetal brains (one per litter) using TRIzol Reagent as per the TRI Reagent Protocol (Invitrogen), and delivered to the Genomics Shared Resource lab at The Ohio State University Comprehensive Cancer Center ...
-
bioRxiv - Molecular Biology 2021Quote: Samples were cooled to room temperature and supernatant was ran on Novex NuPAGE 4-12% Bis-Tris Gel (NP0321, Invitrogen). Samples were transferred to nitrocellulose membrane ...
-
bioRxiv - Immunology 2020Quote: ... HepG2 or Vero E6 cells were plated in a 12-well plate at 5 x 10E5 cells/well in DMEM medium (Gibco) supplemented with 5% FCS ...
-
bioRxiv - Immunology 2020Quote: ... or homogenized with a Bio-Gen PRO200 homogenizer with an attached 5mm X 75mm flat bottom generator probe (Pro-Scientific) and then loaded into 4-12% Bolt Bis-Tris gels (Invitrogen) for gel electrophoresis ...
-
bioRxiv - Immunology 2021Quote: ... Equal amounts of protein were electrophoresed on either 4-12% Bis-Tris or 10% Bis-Tris gels (NuPAGE, Life Technologies) and wet-transferred to nitrocellulose membranes ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were sonicated and aliquots containing 5 x 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen) under reducing conditions ...
-
bioRxiv - Developmental Biology 2021Quote: ... retinal aggregates were picked up and transferred in 60-mm Petri dishes in maturation medium composed of Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 with glutamax (Thermo Fisher Scientific), 1% of N-2 supplement (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... The concentrated samples were boiled in a reducing sample buffer and separated in 4-12% polyacrylamide gel (#NW04122BOX, Thermofisher Scientific) electrophoresis (PAGE ...
-
bioRxiv - Neuroscience 2020Quote: ... Supernatants containing the soluble proteins were subjected to SDS-PAGE on NuPAGE™ 4–12% Bis-Tris Protein Gels (Invitrogen) in the Mini Gel Tank and Blot Module Set (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2021Quote: ... Briefly, lysates were sonicated 12 times (15 sec on, 180 sec off) using a Dismembrator Model 500 sonicator (Fisher Scientific) at 30% output ...
-
bioRxiv - Genomics 2021Quote: ... or non-targeting control (D-001206-14-05) in 12 or 24-well plates using Lipofectamine RNAiMAX reagent (Life Technologies) following the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... L-glutamine 200 mM, Transwell® culture inserts (12 mm diameter, 0.4 μM pore size) and trypsin–EDTA from ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads eluate was heated at 95°C for 10 min and loaded on polyacrylamide gel (4-12% Bis-Tris gel (Invitrogen)) and run in MOPS SDS Running Buffer (Life Technologies) ...
-
bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 2mls of Primary Medium consisting of DMEM/Ham’s F-12 nutrient mix (1:1) (ThermoFisher Scientific), with 10% of fetal bovine serum (FBS) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 0.5 x 106 mESCs were then plated to gelatin-coated T25 flasks and grown in N2B27 media (50:50 DMEM/F-12:Neurobasal (Gibco) supplemented with 1 X N2 and 1 X B27 (ThermoFisher) ...
-
bioRxiv - Biochemistry 2021Quote: The KDELR/Legobody complex at 0.8mg/ml was PEGylated by incubation with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at a 1:40 molar ratio for 2 hrs on ice to reduce preferred particle orientation on the grids ...
-
bioRxiv - Microbiology 2020Quote: ... 2 μg of SOSIP trimer or 3.2 μg of SOSIP-I53-50NP (which is equivalent to 2 μg of trimer) were loaded on a 4-12% Tris-Glycine gel or 6-18% Tris-Glycine gel (both from Invitrogen). For BN-PAGE ...
-
bioRxiv - Cancer Biology 2020Quote: Six metastatic patient-derived samples were treated in adherence for 72h in the presence of 5 μM SFX-01 or water (vehicle control) in DMEM/F-12 GlutaMAX medium (GIBCO) containing 10% foetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2023Quote: ... The pelleted cells were washed by pre-warmed mTEC/Basic medium containing advanced DMEM/F-12 (Life Technologies, #12634-028), 15mM HEPES (Life Technologies ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... 5% Coomassie brilliant blue G-250 was added before loading ~100 μg on a 3-12% Bis-Tris BNE gel (Invitrogen). After electrophoresis (2.5 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was sectioned using a freezing cryostat at 12 µm and mounted directly onto Superfrost Plus Gold slides (Fisher Scientific) and then stored at −80°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein and its purity were checked at each step of the purification by SDS-PAGE electrophoresis (4-12% Bis-Tris NuPAGE, MES as running buffer, Invitrogen) stained with SimplyBlue SafeStain (Novex ...
-
bioRxiv - Molecular Biology 2022Quote: ... An equal amount of proteins per sample were resolved by SDS- PAGE on NuPAGE Bis-Tris gels (4-12%) (Invitrogen), followed by transfer onto 0.2 μm Nitrocellulose membrane using a Trans-Blot Turbo Transfer System (Bio-Rad) ...
-
bioRxiv - Immunology 2023Quote: ... added with 50 mM DTT for 10 min at 75 C and loaded onto precast NuPAGETM 4-12% bis-tris protein gels (Invitrogen), or NuPAGETM 12% bis-tris protein gels (these ones were used for LC3 immunoblotting ...
-
bioRxiv - Cell Biology 2023Quote: HEK 293T cells transduced with the FlexNanoluc construct were seeded in the bottom chamber of 12-well plates at 100,000 cells/well in DMEM (Thermo Fisher) supplemented with 10% FBS (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and were heated at 95°C for 10min prior to resolving on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MOPS SDS Running Buffer (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 µl of the eluted proteins were separated by migration on a NuPAGE 4–12% Bis- Tris Protein Gel (Invitrogen) and silver stained ...
-
bioRxiv - Immunology 2022Quote: ... two μg samples (2/12 μL) were injected on a 50 cm long EASY-Spray C18 column (Thermo Fisher Scientific) connected to an Ultimate 3000 nanoUPLC system (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... isolated primary cells were co-cultivated with gamma-irradiated mitotically inactivated NIH3T3 mouse embryonic fibroblasts (MEFs) in a 3:1 mixture of Ham’s F-12 nutrient mix (Life technologies) and DMEM supplemented with 5 % FCS ...
-
bioRxiv - Microbiology 2023Quote: ... and both elutions were examined by SDS-PAGE by loading samples in a NuPAGE 4-12% Bis-Tris precast gradient gel (Invitrogen) using MES SDS buffer.
-
bioRxiv - Immunology 2023Quote: ... 10 μL of PageRuler Protein Ladder and 7 μg of each sample of proteins mixed with 5 μL of loading buffer were loaded and separated on a NuPAGE 4 to 12% Bis-Tris Gel (Invitrogen) using MOPS SDS Running Buffer 20X (Life technologies ...
-
bioRxiv - Neuroscience 2023Quote: ... 40ug of protein was loaded on SDS PAGE (4-12% Novex NuPAGE) followed by transfer onto nitrocellulose membrane using iBlot (Invitrogen). For proteins < 100 kDa ...
-
bioRxiv - Physiology 2023Quote: ... Worms were then incubated overnight in the dark at room temperature with 33 µM SYTO 12 Green fluorescent nucleic acid stain (ThermoFisher) in NGM and a small amount of E ...
-
bioRxiv - Physiology 2023Quote: ... 36 hours upon transfection DMEM-F12 medium was replaced with fresh DMEM/F-12 medium without phenol red (Gibco #21041025). 48 hours post transfection ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were mixed using either standard SDS Lämmli sample buffer and heated at 80°C for 5 min prior to loading onto gels (Novex Nupage 4-12% Bis- Tris gels, 1mm, 15 well, Invitrogen) and ran with Novex Nupage MES-SDS buffer ...
-
bioRxiv - Microbiology 2023Quote: ... Protein samples were first purified by running them a short distance into a 4-12% NuPAGE Novex Bis-Tris Minigel (Invitrogen), followed by Coomassie staining and in- gel digestion with trypsin (96).
-
bioRxiv - Plant Biology 2023Quote: ... samples were loaded and run on Native PAGE 3%-12% Bis-Tris gels alongside either NativeMark unstained protein standard (Invitrogen) or SERVA Native Marker (SERVA) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Microdissected areas were digested with 12 ml of Direct Reagent (Ion AmpliSeq™ Direct FFPE DNA Kit, MAN0014881, ThermoFisher Scientific) for 30 min at 65 C ...
-
bioRxiv - Physiology 2023Quote: ... and subsequently incubated with antibodies for intracellular markers or corresponding isotype controls (IL33-PE 1:200 IC3626P R&D, FOXP3-PE 1:200 12-5773-82 Thermofisher, Isotype-PE 1:200 12-4321-83 Thermofisher ...