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Citations for Thermo Fisher :
5751 - 5800 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... to E18 cortical neurons were cultured on glass coverslips coated with poly-D-lysine in Neurobasal medium with B27 supplement (Invitrogen) containing 1% penicillin/streptomycin and 2mM glutamine ...
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bioRxiv - Plant Biology 2021Quote: ... and the coding sequences of three TFs (VviMYB24, AtMYC5, and VviMYC2) were amplified and cloned into the entry vector pENTR/D-TOPO (Invitrogen) and then transferred by site-specific recombination into the reporter and effector vectors (pPGWL7.0 and pK7WG2.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Murine cell lines were cultured in Dulbeccos’ Modified Eagle Medium/F12 medium supplemented with 5 mg/mL D-glucose (Invitrogen), 0.1 mg/mL soybean trypsin inhibitor type I (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... and the cells were plated on 50 μg/ml poly-D-lysine-coated cover glasses in Neurobasal medium (Biological Industries, Israel) supplemented with 2% B-27 (Invitrogen), GlutaMAX-I 2mM (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The Stim coding sequence was cloned by PCR using primers 5’-CAC CAT GCG AAA GAA TAC CAT TTG GAA C-3’ and 5’-TTC CGT GGC AAG CAG CGA AAA GTT C-3’ and ligated into pENTR/D-TOPO (Invitrogen). Site-directed mutagenesis (Stratagene QuikChange XL ...
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bioRxiv - Cell Biology 2021Quote: ... Amplified sequences were cloned by BR reactions in entry vectors pDONR™221 or pENTR-d-TOPO™ (Thermofisher Scientific) using Gateway® recombinational cloning technology (Thermofisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... The cell culture plates were coated with 0.5 ml of poly-D-lysine (Sigma-Aldrich) (0.1 mg/ml in no calcium, no magnesium Dulbecco's Phosphate-Buffered Saline (DPBS) (Life Technologies)) per well for 30 minutes ...
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bioRxiv - Immunology 2020Quote: Cell survival was determined by analyzing forward/sideward scatter properties of cells and simultaneous staining with either Propidium iodide (PI), or 7-amino-Actinomycin D (7AAD eFluor670 dye, and LIVE/DEAD fixable violet stain (ThermoFisher), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and ON-TARGETplus SMART pool siRNA oligonucleotides and ON-TARGETplus NON-targeting pool (D-001810) (Dharmacon) were transfected with 25 or 50 nM siRNA by Lipofectamine RNAimax (Invitrogen) for 48 to 72 h following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The OP9 stromal cell line (D. Allman, University of Pennsylvania) was grown in DMEM supplemented with 10% FBS (Life Technologies), 100 U/ml penicillin-streptomycin ...
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bioRxiv - Cell Biology 2021Quote: U-2 OS cells (human bone osteosarcoma epithelial cells, ATCC Number: HTB-96) (88) were grown in D-MEM Glutamax (Gibco) supplemented with final concentrations of 10% fetal calf serum (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with siHMOX2 (Dharmacon L-009630-00-0005) or non-targeting pool (Dharmacon D-001810-10-05) siRNA using Lipofectamine RNAi Max (Thermofisher). Briefly ...
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bioRxiv - Genomics 2021Quote: ... or non-targeting control (D-001206-14-05) in 12 or 24-well plates using Lipofectamine RNAiMAX reagent (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... The recombination reaction from pENTR/D-TOPO to the pGWB602 binary vector was carried out with the Gateway LR clonase II system (Invitrogen). All primers used in the construction of the AtCPT3 silencing vector are listed in Supplementary file 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with either non-targeting control (Dharmacon, D-001810-10-05) or Mage-b4/10 siRNA oligonucleotides by Lipofectamine 3000 (Invitrogen) as follows ...
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bioRxiv - Immunology 2022Quote: Cells prepared from the milk and blood samples were suspended in D-PBS (wo Ca/Mg) containing 10% (v/v) of horse normal serum (Gibco) and 2mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... the PCR amplified ΔNHT2 S6K1 was sub-cloned into TOPO adapted entry vector using pENTR/TEV/D-TOPO Cloning Kit (Invitrogen). The entry vector was then recombined with pWZL-Neo-Myr-Flag-DEST Gateway-compatible retroviral destination vector (addgene# 15300 ...
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bioRxiv - Biophysics 2022Quote: ... Upon reaching an OD600 of 0.6 – 0.8 the cells were induced with 1 mM IPTG (isopropyl β-D-1-thiogalactopyranoside; Thermo Scientific) and expression was continued overnight (12–16 h ...
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bioRxiv - Biophysics 2022Quote: ... 0.020 M potassium phosphate monobasic), sodium chloride (BioXtra grade, purity ≥99.5%) and D-(-)-Fructose (HPLC grade, purity ≥99%) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Developmental Biology 2022Quote: ... GAL4-GR UAS was PCR-amplified from pDONOR 221 GAL4::GR::UAS::GFP::UAS [56] and cloned into the D-TOPO vector (Invitrogen). The SHR CDS was PCR-amplified from a pDONR221 SHR vector ...
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bioRxiv - Genomics 2022Quote: ... Liver spheres were collected and sorted in low glucose DMEM (1g/L D-Glucose/L-Glutamine, Pyruvate, Gibco #31885-023). The same filtering approach was used for Crohn’s disease biopsies with a lower size strainer of 200 μm and a higher size strainer of 500 μm resulting in a suspension of spheres between 200-500 μm ...
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bioRxiv - Genomics 2022Quote: ... Liver dissociation buffer was a mix of low glucose DMEM (1g/L D-Glucose/L-Glutamine, Pyruvate, Gibco #31885-023), 15mM HEPES (Gibco ...
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bioRxiv - Plant Biology 2022Quote: A 5.2 kb genomic fragment of AAR2 containing the promoter and coding region without the stop codon was PCR-amplified and cloned into pENTR/D-TOPO (Invitrogen) and then introduced into pGWB40 (60 ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fragmented and primed mRNA was further subjected to the first-strand synthesis in Actinomycin D (Gibco, Life Technologies, CA, USA), followed by second-strand synthesis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fragmented and primed mRNA was further subjected to the first-strand synthesis in Actinomycin D (Gibco, Life Technologies, CA, USA), followed by second-strand synthesis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fragmented and primed mRNA was further subjected to the first-strand synthesis in Actinomycin D (Gibco, Life Technologies, CA, USA), followed by second-strand synthesis ...
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bioRxiv - Plant Biology 2022Quote: The RBP45d coding DNA sequence (CDS) and PRP39a genomic DNA were cloned into the pENTR SD/D-TOPO entry vector (Invitrogen), and then recombined into the pGWB2 destination vector ...
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bioRxiv - Plant Biology 2022Quote: ... The amplified and purified PCR product was first cloned into a gateway pENTR/d-TOPO entry vector (Invitrogen, Life Technologies) and were subsequently cloned in pKGWFS7 ...
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bioRxiv - Plant Biology 2022Quote: ... The amplified and purified PCR product was first cloned into a gateway pENTR/d-TOPO entry vector (Invitrogen, Life Technologies) and were subsequently cloned in pKGWFS7 ...
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bioRxiv - Plant Biology 2022Quote: ... in the pENTR/D-TOPO vectors were all cloned into pMDC123 destination vector via LR reaction using Gateway LR Clonase II (Invitrogen). pEG302-EFFECTOR (gDNA)-3XFLAG-ZF is also a Gateway-compatible binary destination vector ...
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bioRxiv - Plant Biology 2022Quote: ... The sequence of native promoter (~1.5kb upstream from the 5’UTR or until the next gene annotation) and genomic DNA (No stop codon) of the effectors were cloned into pENTR D-TOPO vectors (Invitrogen), which were used to deliver the genomic DNA sequences of these effectors into the destination vector using Gateway LR Clonase II (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... hiPSC-CMs were metabolically enriched for 5 d with daily feeding with DMEM without glucose or L-glutamine (Invitrogen, F530S) supplemented with 4 mM Sodium L-lactate (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... genomic DNA fragments including the promoter region were amplified and cloned into entry vectors (pENTR-D or PCR8 from Invitrogen) and cloned into destination vectors with C-terminal 3xFLAG (pEG302_GW_3xFLAG) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured according to manufacturer’s guidelines in Dulbecco’s Modified Eagle’s Medium (D-MEM) that was supplemented with 10% fetal bovine serum (FBS) and Penicillin-Streptomycin (all ThermoFisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... 5 mM EDTA and 1% BSA in HBSS) and 2μL of 7-aminoactinomycin D (7-AAD, Thermo Fisher Scientific, A1310) at a concentration of 0.1 mg/mL were added ...
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bioRxiv - Neuroscience 2022Quote: ... Cortices were triturated in plating medium with a p1000 pipette then transferred to a T-25 cell culture flask coated with Poly-D-Lysine (Gibco) with additional glial media for a total volume of 4 mL ...
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bioRxiv - Neuroscience 2022Quote: ... Isolated DRG neurons were seeded on glass coverslips coated with 0.1 mg/mL Poly-D-lysine (PDL, Gibco Cat#A3890401), followed by coating with either 10 μg/mL Laminin (ThermoFisher Cat#23017015) ...
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bioRxiv - Neuroscience 2022Quote: ... centrifuged at 120 g for 10 minutes to remove enzymes and lysed cells and resuspended in Glial Medium (Minimal Essential Medium with Earl’s Salt and L-glutamine, 0.6% D-glucose, 100 U/ml Penicillin-Streptomycin, 10% fetal bovine serum; Invitrogen, Carlsbad, USA). Glial cells were plated in 75-cm2-flasks (Falcon ...
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bioRxiv - Microbiology 2022Quote: ... the inoculum was removed and cells were passaged in 10% FBS D-MEM supplemented with 50 µg/ml puromycin (Gibco) to select for transduced cells ...
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bioRxiv - Microbiology 2022Quote: ... were excised from the growing edge of the colony and submerged in 50 μl of water or indicated phytoalexin containing 50 μM D-luciferin in a sealed 96-well microplate (Nunc 96F microwell white polystyrene plate ...
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bioRxiv - Plant Biology 2022Quote: ... was amplified from cDNA derived from Col-0 plant and cloned into the gateway entry vector pENTR/D-TOPO (Invitrogen) and then transferred into the destination vector pGWB605 vector62 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fragmented and primed mRNA was further subjected to the first-strand synthesis in Actinomycin D (Gibco, Life Technologies, CA, USA), followed by second-strand synthesis ...
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bioRxiv - Plant Biology 2022Quote: ... and gSAID1-pENTR/D was assembled with the modified vectors above by Gateway attL-attR (LR) recombination reaction (Thermo Fisher) to yield final binary vectors ...
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bioRxiv - Neuroscience 2024Quote: ... Cell suspensions were washed twice and resuspended in 1 mL of Dulbecco’s PBS (D-PBS) before viability staining using Live/Dead™ Fixable Aqua (1/1000; ThermoFisher) according to manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... samples were incubated with 5 µg/mL of the DyLight488-conjugated anti-FLAG M1 antibody and the viability dye 7-Aminoactinomycin D (7-AAD, Invitrogen) for 45 min on ice and were then analyzed on a Guava easyCyte cytometer (Millipore) ...
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bioRxiv - Plant Biology 2024Quote: ... a genomic fragment containing the NRL5 gene body up to the stop codon and 2 kb of upstream of promoter sequence was amplified from Col-0 and cloned into pENTR/D-TOPO (Invitrogen). Site-directed mutagenesis of this clone was conducted to generate a genomic clone of NRL5P335L using the QuikChange II site-directed mutagenesis kit (Agilent ...
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bioRxiv - Plant Biology 2024Quote: Full-length AtMED25 and OsMED25 coding sequences and the full-length AtMED25 promoter were cloned into pENTR-D/TOPO (Invitrogen) and recombined either into pK7WG2 (AtMED25 and OsMED25 coding sequences ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthetized from 2ug of total RNA using MMLV-Reverse transcriptase and d(N)6 random primers in a 20ul reaction following the manufacturer’s protocol (ThermoFisher Scientific). RT-qPCR analyses were performed in triplicate using 2.5 μl of cDNA ...