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Citations for Thermo Fisher :
351 - 400 of 1270 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... HeLa cells were transfected at 30-40% confluency using Lipofectamine RNAiMax (Invitrogen, #13778-150) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... polyadenylation (150-200 nt) was achieved using the Poly(A) Tailing Kit (Ambion, AM1350). All messages were purified using the MEGAclear Kit (Ambion ...
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bioRxiv - Genomics 2023Quote: ... Cells were resuspended in 150 µl of cold radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher) and incubated on ice for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was poured onto an empty plate (150 x 15 mm, Fisher Scientific) and allowed to solidify ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 150 μL of OptiMEM containing 9 μL of LTX reagent (Thermo Fisher). The cDNA/Lipofectamine mixture was incubated for 7 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... 150 μL of 10 mg/mL Dynabeads MyOne Streptavidin T1 beads (Life Technologies, 65602) were separated on a magnetic stand ...
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bioRxiv - Neuroscience 2024Quote: ... IBA-1 (ionized calcium-binding adapter molecule 1; antibody PA5-21274, Invitrogen; 1:150) and astrocytes (anti-S100 beta antibody ...
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bioRxiv - Microbiology 2023Quote: ... 150 µL freshly prepared No-Weigh Sulfo-NHS-LC-LC-Biotin (Thermo Scientific A35358) was added and the samples were incubated ...
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bioRxiv - Molecular Biology 2023Quote: ... which was then transfected into mESCs using the RNAiMAX transfection reagent (Invitrogen #13778-150). After 48h incubation ...
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bioRxiv - Bioengineering 2024Quote: ... sequencing libraries were prepared from 150 ng genomic DNA quantified by Picogreen (Life Technologies) using the Lotus DNA Library Prep Kit (Integrated DNA Technologies ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... 150 mM NaCl) in the presence of Protease Inhibitor Cocktail (Thermo Fisher Scientific 78425), Phosphatase Inhibitor Cocktail 2 (Sigma P5726 ...
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bioRxiv - Microbiology 2022Quote: ... in OPTI-MEM I Reduced Serum Medium (150 μl) (Thermo Fisher Scientific, cat. #31985062). The mixture was transfected into BHK-T7 cells seeded in 12 well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... and run at 150 V in 1x MOPS SDS running buffer (Thermo Scientific, NP0001). Protein was then transferred from the gel onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: Frozen brown adipose tissue was homogenized in 150 µL cold M-Per (Thermo Fisher) containing 1:50 HALT Protease and Phosphatase Inhibitor Cocktail (EDTA-free ...
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bioRxiv - Genetics 2022Quote: ... 150 ng total RNA was reverse transcribed using SuperScript IV Reverse Transcriptase kit (Invitrogen) with random primer mix ...
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bioRxiv - Genetics 2023Quote: ... 150 mM β-mercaptoethanol) and quantified using Pierce BCA Protein Assay Kit (Thermo Scientific). Extracts were analysed by SDS–polyacrylamide gel electrophoresis using 4-12% Bis-Tris NuPAGE protein gels (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... containing 200 μg/mL G418 (Carl Roth) and 150 μg/mL Hygromycin B (Invitrogen). NIH3T3 and NIH3T3mFAP cell lines were generated as described previously40 and kindly provided by Prof ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid DNA (150 ng/ well) was added to the Opti-MEM (Invitrogen; 31985088), followed by the addition of 1.25 μg/well of PEI lipid and incubated at room temperature for 30 min before being added to cells.
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bioRxiv - Immunology 2023Quote: ... and cryopreserved at −150°C in 40% heat-inactivated fetal bovine serum (FCS, Gibco), 50% RPMI-1640 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... single cells were collected by trypsinization and resuspended in 150 µl EBSS (Thermo Fisher) containing 5 µM C11-BODIPY 581/591 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... 150 ul of Dynabeads™ Protein G was used for Immunoprecipitation (Invitrogen, Cat.No. 10004D) per ChIP reaction ...
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bioRxiv - Cell Biology 2024Quote: ... C2C12 cells on 6-well plates were transiently transfected with RNAiMAX (Invitrogen, 13778-150) according to the manufacturer’s directions in two stages to prevent over-confluency at the time of cellular treatment and lysis ...
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bioRxiv - Biophysics 2024Quote: ... at 150 rpm inside an environmental chamber (Thermo Scientific, Catalog no. 13-067-066). YPD media was made with 5 g of yeast extract (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Centrifugation of protoplasts was performed at 150 RCF (Thermo Scientific Megafuge ST4R Plus-MD). Following centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: 150 ng of DNA template was transcribed using a MEGAscript T7 Transcription Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Exponentially growing cells were incubated with 150 μM of EdU (Click-iT; Thermo Scientific) for 30 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended with 150 µL of Trypsin/Lys-C (4 µg, Thermo Fisher Scientific) in 200 mM EPPS (pH 8.4)/5 mM CaCl2 (Sigma Aldrich) ...
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bioRxiv - Plant Biology 2024Quote: ... mRNAs were isolated using 150 µL magnetic Oligo(dT)25 beads (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... according to manufacturer’s instructions and selected in Hygromycin B (150 µg/mL) (Thermo Scientific). Protein expression was induced by overnight incubation with doxycycline (DOX ...
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bioRxiv - Developmental Biology 2024Quote: ... 150 μl of whole blood was collected in heparinized micro-hematocrit tubes (Fisher Scientific) for measurement of serum insulin levels ...
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bioRxiv - Cancer Biology 2021Quote: Flash frozen tumor pieces were homogenized using Fisher Homogenizer 150 (Thermo Fisher, Waltham, MA, USA). Total RNA was isolated using QIAGEN RNeasy Midi Kit (#75142 ...
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bioRxiv - Cancer Biology 2021Quote: Flash frozen tumor pieces were homogenized using Fisher Homogenizer 150 (Thermo Fisher, Waltham, MA, USA). Total RNA was isolated using QIAGEN RNeasy Midi Kit (Hilden ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Final samples were eluted two times in 150 µl warm dH2O (Invitrogen Cat #: 10977-015). A modified Canadian Center for DNA Barcoding (2020 ...
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bioRxiv - Cancer Biology 2022Quote: About 150–200 ng genomic DNA (measured with Qubit dsDNA HS assay kit, ThermoFisher Scientific), were sheared by the Sure Select Enzymatic Fragmentation kit (Agilent Technologies Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... the adjacent sections were incubated with NeuroTrace 500/525 (1:150; N-21480, Life Technologies), a green fluorescent Nissl stain ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate was precleared with 150 µl of Pierce control agarose resin (Thermo Fisher Scientific) equilibrated with hypotonic lysis buffer at 4°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... run for 50 min at 150 V in 1X MES-SDS running buffer (Invitrogen, NP0002). Novex™ Sharp Pre-stained protein standard (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5 μg of DNA per well was mixed with 150 μl of OptiMEM (Life Technologies) media and incubated for 5 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... adsorbed to 150 µg of aluminum hydroxide (imject alum, Thermo Fisher Scientific, Waltham, MA, USA) on days 1 ...
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bioRxiv - Microbiology 2021Quote: ... Sample was resolved using a 75 μm x 150 cm PepMap C4 column (Thermo Scientific). MS spectra of protein ions of different charge-states were acquired in positive ion mode with a resolution setting of 120,000 (at m/z 200 ...
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bioRxiv - Cell Biology 2020Quote: ... with 1 % Penicillin/Streptomycin on Geltrex™ (Gibco™, 1:150 dilution in DMEM/F12) coated 35 mm dishes at 37 °C in 5 % CO2 atmosphere ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl and one tablet of protease and phosphatase inhibitors (A32959, Thermo Fisher Scientific) for 30 min on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7.2 and 150 mM NaCl buffer for purification on Protein A Sepharose 4B (ThermoFisher). The protein was eluted in 0.1 M citric acid ...
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bioRxiv - Physiology 2022Quote: ... 150 to 300 ng of total RNA determined by Qubit (Cat#Q33238; Invitrogen, Waltham, MA) high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595 ...
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bioRxiv - Microbiology 2022Quote: ... Absorbance of indophenol blue was quantified in a Genesys 150 UV visible spectrophotometer (Thermo Scientific) at 652λnm after 4-h incubation at room temperature.
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bioRxiv - Neuroscience 2022Quote: hOPCs were seeded at 5 × 104 cells/mL and Lipofectamine RNAi max (Invitrogen, #13778-150) was used to transfect cells according to the manufacturer’s protocol with minor alterations ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T cells (5 × 106) were seeded in 150 mm plates with Opti-MEM (Gibco; 51985091). After 24h ...
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bioRxiv - Developmental Biology 2022Quote: ... 150 μl of streptavidin magnetic beads (10 mg/mL Dynabeads M-280, Invitrogen, Carlsbad, CA) were washed three times with 1X PBS 0.05% Tween ...
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bioRxiv - Microbiology 2020Quote: Whole blood (50 µL) was resuspended in 150 µL of TRIzol LS Reagent (Life Technologies). All Organs were added to Omni pre-filled bead tubes containing 1mL of TRIzol and homogenized using the OMNI bead ruptor 12 ...
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bioRxiv - Biochemistry 2020Quote: ... at 150 V for 1 hour then switched to light blue cathode buffer (Invitrogen, BN2002) and run at 30 V overnight ...