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Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% FBS (Biosera; FB-1058) and 2 mM GlutaMAX™-1 (Gibco; 35050). SupT1 (ECACC ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Immunology 2022Quote: ... and stimulated in B cells complete medium (R10, RMPI 1640 10% FBS Hyclone complete medium) supplemented with ITS (1:100, Thermo Fisher, # 41400-045). To stimulate murine B cells ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% foetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (PSA: Penicillin, Streptomycin and Amphotericin B) (Thermo Fisher Scientific, Waltham MA, USA).
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bioRxiv - Microbiology 2020Quote: The concentration of culture supernatants and MMP-9 were measured by Human MMP-9 ELISA Kit (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 10 μl of the resulting 1:10 dilution were mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher) and subjected to library preparation for next-generation sequencing (vertis Biotechnologie) ...
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bioRxiv - Genetics 2021Quote: ... containing 10% 2-propanol (ThermoFisher Scientific, UK). Membranes containing transferred protein were washed in Tris-Buffered Saline (20mM Tris ...
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bioRxiv - Genomics 2020Quote: ... 2 ul 10× UDG buffer (Thermo Scientific), 1 ul (1U/ul ...
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bioRxiv - Neuroscience 2022Quote: ... 55 uM 2-mercaptoethanol (Gibco, 31350-10) and 100 U/mL penicillin/streptomycin (Biological Industries ...
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bioRxiv - Neuroscience 2022Quote: ... 55 uM 2-mercaptoethanol (Gibco, 31350-10), 50 U/mL penicillin/streptomycin (Biological Industries ...
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bioRxiv - Developmental Biology 2022Quote: ... Fura-2 (final concentration 10 µM, Invitrogen) was administered to dissected cochlear spirals bathed in extracellular medium containing 0.2% F-127 (Invitrogen ...
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bioRxiv - Systems Biology 2022Quote: ... RNaseOUT inhibitor—2 µL/10 mL (Invitrogen)) and ice incubated for five minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 ng/ml IL-2 (Gibco) for 96 hours at 37 LCMV infection and 5 % CO2.
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bioRxiv - Cell Biology 2023Quote: ... 2 mM Glutamax and 10% FCS (Invitrogen) in humid environment containing 5% CO2.
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated in primary antibody overnight at 4°C (rabbit anti-GFP 1:1000; Invitrogen A-6455 RRID:AB_221570), and in secondary antibody at 1:500 for 3 hours at room temperature goat (anti-rabbit Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2022Quote: ... at 4°C overnight followed by donkey anti-rabbit Alexa 488 secondary antibody (1:1000; A32766, Thermo Fisher Scientific), donkey anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were first incubated overnight at 4°C in rabbit anti-CALR (1:500, ThermoFisher, Waltham, MA, cat# PA3900) then goat anti-rabbit Alexa Fluor 488 (1:2000 ...
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bioRxiv - Immunology 2019Quote: ... loaded onto NuPAGE® 4-12% gels and run at 120 V in 1 X MES buffer (ThermoFisher Scientific). The separated proteins were transferred onto membranes using the iBlot® transfer system (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... at 4 °C followed by incubation the next day with goat anti-chicken antibody (647nm, A21449, ThermoFisher, 1:600) and goat anti-rabbit antibody (568 nm ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with primary antibodies (in 0.01 M PBS with 1% BSA and 0.3% Triton X-100) overnight at 4 °C and subsequently with corresponding secondary antibodies (1:1,000, Alexa-Fluor-conjugated, Invitrogen). DAPI was used to label the nuclei and sections were mounted with 75% glycerol ...
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bioRxiv - Biophysics 2020Quote: ... cells were previously fixed with paraformaldehyde 4% and permeabilized with Triton-X100 (0.5%) in PBS and stained with 1/200 anti-E-cadherin-FITC (Invitrogen).
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bioRxiv - Bioengineering 2021Quote: ... The resulting supernatant was centrifuged at 20,000 ×g for 1 hour at 4°C (Sorvall WX 100+ Ultracentrifuge, ThermoFisher) to pellet large EVs and the remaining supernatant was again centrifuged at 100,000 ×g for 1 hour at 4°C to pellet crude small EVs ...
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bioRxiv - Bioengineering 2020Quote: ... 1×1010 sEV were incubated with 3.8 µm aldehyde/sulfate latex 4% (m/v) beads (Molecular probes, Eugene, OR). Coated beads were then incubated with anti-human CD63 (eBioscience ...
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bioRxiv - Bioengineering 2020Quote: ... 1-mm coronal sections from each tissue (4-mm for the brain) were homogenized in Trizol (Life Technologies, 15596) using a BeadBug (Benchmark Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated overnight at 4 °C with primary antibody against ZO-1 (7.5 μg/ml, Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Microbiology 2021Quote: ... AGS cells were transiently transfected for 24 h with the sgRNA-containing pX459 vector using polyethylenimine (PEI) at a 4:1 PEI:DNA ratio in Opti-MEM I Reduced Serum Media (OptiMEM; Gibco). Puromycin treatment (2 µg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... Tubes were incubated for 1 hour at 4°C and then protein A/G agarose magnetic beads (Thermo Scientific) were added for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Cell Biology 2020Quote: ... The organoids were incubated overnight at 4◦C with a primary antibody against KI67 (Invitrogen #MA5-14520; 1:200) or MUC2 (Santa Cruz #sc-15334 ...
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bioRxiv - Neuroscience 2020Quote: ... TRA-1-60) in iPSCs was performed using the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Invitrogen™, A24881). iPSC colonies were fixed for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... islets were similarly incubated for 1 hr in imaging buffer at 37 °C with the addition of 2.5 μM Fluo-4 (Invitrogen). Islets incubated with Fluo-4 were washed three times with imaging buffer prior to imaging ...
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bioRxiv - Microbiology 2022Quote: ... lipid concentrations of FtsA fractions were determined by incubation of FtsA with FM 4-64FX (1.25 μg ml-1) (Invitrogen). Fluorescence intensity was measured with an Agilent Eclipse spectrofluorometer using an excitation wavelength of 565 nm and an emission wavelength of 745 nm (slit widths 5/10 nm) ...
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bioRxiv - Biochemistry 2022Quote: ... Sections were incubated overnight at 4 degrees with anti-ATF4 (10835-1-AP, ThermoFisher, Waltham, MA USA, RRID: AB_2058600) antibody at a 1:200 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... the media in each plate was replaced with 40 μL of 1× Hank’s Balanced Salt Solution (HBSS) containing 20 mM HEPES pH 7.4 and 4× dilution of Fluo4 (Thermo Fisher). The assay plate was incubated at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were precleared by incubation for 1 hour at 4°C on a rotator with 10μl Protein G magnetic beads (ThermoFisher). The indicated antibody was incubated in 600μl IP buffer containing 1μl 10mg/ml acetylated BSA and 5μl Protein G magnetic beads for 4 hours with rotation at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Crosslinking was reversed by incubating the beads at 65°C for 4 hr in the presence of 0.3% SDS and 1 mg/ml of Proteinase K (Ambion). DNA was purified using Zymo ChIP DNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then incubated overnight at 4°C with a 1:500 dilution of rabbit anti-HA antibody (Invitrogen) in PBS 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... 6.25 µl of 4 μM primers P1 and P2 and 1 µl of template DNA (Thermo Scientific, cat#F553S), and milliQ water up to 50 μl ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and with anti-rabbit IgG Alex Fluor 633 (ThermoFisher, Cat# A-21070, dilution 1:2000) for one hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... lysate from mock transfected cells was incubated at 4°C for 1 hour with Sepharose-G beads (Fisher Scientific), previously washed with 5% BSA in TBS-Triton buffer and preincubated with 2.5 μg anti-GFP antibody ...
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bioRxiv - Genetics 2022Quote: Immunoprecipitations were performed using 1 μg of Anti-Trimethyl-Histone-3-Lys-4 (-Thermo Fisher Scientific; catalog# PA5-17420) overnight at 4 ◦ C ...
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bioRxiv - Cell Biology 2019Quote: ... the viral medium was them replaced with induction medium composed of DMEM/199 (4:1) (Gibco, Thermo Fisher Scientific) containing 10% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... the viral medium was them replaced with induction medium composed of DMEM/199 (4:1) (Gibco, Thermo Fisher Scientific) containing 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... at 14000 × g at 4 °C for 1 min and loaded into an HPLC system (Thermo Scientific Ultimate 3000). The mobile phase was 12.5% acetonitrile buffer (pH 3.0 ...
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bioRxiv - Neuroscience 2019Quote: Tissue from anonymized donors was received at 4°C in 1 ml “fibroblast medium” containing MEM alpha (Gibco, #12571), 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... and complemented with 7.5 μM of Site2hp RNA (4:1 protein:RNA molar ratio) in PBS buffer in a 384 black-well clear-bottom plate (Nunc). A ThT stock was prepared as described above and added to the well to 300 μM final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... first dimensional lanes are cut from the gel and incubated in 1% SDS with 1% beta-mercaptoethanol for 1 hour before the gel slice was loaded into an NuPAGE 4-13% 1.0mmx2D gel (Invitrogen). Gels were run at 200V for 50 minutes at room temperature in MOPS ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with primary antibodies (anti-ACE2 (1:1000; MA5-32307 - Thermo Fisher), anti-Sigma-1 R (1:500 ...