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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... washed with complete DMEM (DMEM + 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES, 200 U/mL penicillin/streptomycin, 10% FBS; GIBCO) and resuspended in complete DMEM in 15 cm tissue culture plates overnight ...
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bioRxiv - Biochemistry 2022Quote: ... were seeded in a 10- cm culture dish at a concentration of 2 x 105 cells ml-1 (10 ml per dish in DMEM (Nissui) supplemented with 10% fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Biophysics 2021Quote: ... were seeded in a 10-cm culture dish at a concentration of 2 × 105 cells mL-1 (10 mL per dish in DMEM (Nissui Pharmaceutical) supplemented with 10% fetal bovine serum (Gibco), glutamine ...
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bioRxiv - Neuroscience 2019Quote: ... 1 ml of B-27 Supplement (GIBCO, ThermoFisher Cat. #17504044); 1 ml of Penicillin-Streptomycin solution (GIBCO ...
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bioRxiv - Neuroscience 2019Quote: ... 1 ml of B-27 Supplement (GIBCO, ThermoFisher Cat. #17504044); 1 ml of Penicillin-Streptomycin solution (GIBCO ...
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bioRxiv - Bioengineering 2020Quote: ... 1% penicillin-streptomycin-amphotericin B (ThermoFisher Scientific, Waltham, MA; 15240062), 1% Glutamax (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1% penicillin-streptomycin and 1x B-27 Plus (Gibco, A3582801) for 4 h before a complete media change to maintenance medium (plating medium devoid of serum).
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bioRxiv - Cell Biology 2020Quote: ... 1:50 (v/v) B-27 supplement (Thermo Fisher Scientific), 0,5 % (w/v ...
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bioRxiv - Developmental Biology 2022Quote: ... 1× B-27 Supplement Minus Vitamin A (Thermo Fisher Scientific), 2 mM GlutaMAX™ (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% Amphotericin B (fungizone®) (Thermo Fisher Scientific, UK).
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bioRxiv - Cell Biology 2022Quote: ... and 1% Amphotericin B (fungizone®) (Thermo Fisher Scientific, UK) and 1% GlutaMAX™ (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... B-27™ supplement minus insulin (1×, Thermo Fisher Scientific) and CHIR99021 (8 μM ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 1× B-27 Plus supplement (Thermo Fisher Scientific), 0.5% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2022Quote: ... and 1× B-27™ Plus Supplement (ThermoFisher Scientific, A3582801). Unless noted otherwise ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 1% B-27™ supplement (Gibco, # 12587-010), 20 ng/ml recombinant human EGF protein (R&D ...
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bioRxiv - Molecular Biology 2022Quote: ... and 0.25 μg mL−1 amphotericin B (Thermo Fisher Scientific) at 37°C in a 5% CO2 humidified incubator following the supplier’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 1 X B-27 Supplement (Thermo Fisher Scientific, 17504-044) to prepare a basal medium ...
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bioRxiv - Neuroscience 2024Quote: ... B27 media consisting of 1× B-27 supplement (Gibco, 17504044), 1× GlutaMax supplement (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 1× B-27 without Vitamin A (Cat.#12587010, Thermo Scientific) supplemented with 500 ng/ml human Sonic Hedgehog/Shh (C24II ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% B-27 Plus Serum Free Supplements (Gibco, Gaithersburg, MD), 1% Glutamax ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% B-27 Plus Serum Free Supplements (Gibco, Gaithersburg, MD), 1% Glutamax ...
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bioRxiv - Neuroscience 2023Quote: ... 1% B-27 without vitamin A (Thermo Fisher Scientific, 17504044), 1% GlutaMAX ...
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bioRxiv - Microbiology 2023Quote: ... 1 % PS and 250 ng/ml Amphotericin B (ThermoFisher, 15290018) and centrifuged at 4000 rpm for 10 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... TSA AF555 (1:200) (catalog #: B-40955; Thermo Fisher Scientific) was added for 20 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... with B-27 supplement (50:1, Gibco REF 17504-044). HL-1 cells were obtained from the laboratory of William C ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were dehydrated by sequential submersion in 100% ethanol (2× 5 min) and SafeClear II (2× 5 min, Fisher Scientific) before being mounted in Cytoseal 60 (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... and 52h after plating 5 µM 5-ethynyl-2′-deoxyuridine (EdU, Life Technologies) was added for 20 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
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bioRxiv - Biochemistry 2019Quote: ... were made up in HPLC grade H2O (1 mL) immediately before injection onto a ProPac PA-1 analytical column (4 × 250 mm, ThermoFisher Scientific, UK), pre-equilibrated in HPLC grade H2O at a flow rate of 1 mL.min-1 ...
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bioRxiv - Neuroscience 2020Quote: ... OFC sections were reacted with the primary antibody for rabbit met-enkephalin (1:400, Abeam ab22620) for 24h at 4°C and Alexa-fluor 488 goat anti rabbit secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... at 30 V for 1 hour followed by100 V for a further hour at 4°C in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific) supplemented with 0.1 g/l of SDS (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... (alone or in combination) in IF washing buffer overnight at 4°C: anti-ZO-1 rabbit polyclonal (1:200) (Thermo Fisher Scientific), anti-E-cadherin rabbit monoclonal (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 1 mg of protein were incubated with anti-HA antibody (1:100) for 1 h at 4 °C followed by incubation with Protein G Dynabeads (Thermo Fisher Scientific) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... the Transwell inserts were incubated with one of the following primary Abs in IF wash buffer overnight at 4°C: anti-ZO-1 rabbit polyclonal (1:200) (40-2200, Thermo Fisher Scientific), anti-E-cadherin rabbit monoclonal (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... concentration 1:100) for 24 h at 4°C followed by labeling with goat anti-rabbit Alexa fluor 488 (A11034, Invitrogen, 1:1000) as a secondary antibody for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cell Biology 2023Quote: ... and 20% sucrose-PBS and then incubated overnight at 4°C in a 1:1 solution of 20% sucrose and OCT compound (Fisher Scientific: #23730571). The next day ...
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bioRxiv - Developmental Biology 2023Quote: ... were mechanically lysed in [100 μl/sample + 10 μl extra] lysis buffer (100 mM NaCl, 50 mM Tris, 4 mM EDTA, 1% NP40, 1 μl/ml SUPERaseIN (Thermo Fisher #AM2694), and 1x cOmplete Protease Inhibitor (Roche #11836153001) ...
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bioRxiv - Genomics 2023Quote: ... 2.0 mM 2-mercaptoethanol and 0.1 mg/mL-1 bovine serum albumin) and then stopped by adding 4× LDS sample buffer (∼3.3μL) (Thermo Fisher Scientific, no. NP0007) to a final concentration of 1× (final volume ∼13.3μL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining was performed at 1:200 for 30 minutes prior to fixation overnight in 1% phosphate buffered formalin (Fisher Scientific, SF100-4). Note ...
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bioRxiv - Immunology 2023Quote: NK cells were activated by co-culturing NK cells with irradiated K562 cells expressing membrane bound interleukin-21 and 4-1BBL at 1:1 ratio in RPMI (Gibco, Thermo Fisher) supplemented with 10% FCS (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibodies were detected using secondary antibodies (diluted 1:500-1:1000 in PBS/4% BSA) conjugated to Alexa Fluor 568 or 647 (Thermo Fisher Scientific). Cells were counterstained with DAPI and kept in PBS until images were taken with the LSM780 microscope.
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bioRxiv - Evolutionary Biology 2020Quote: ... Cellular debris was removed by centrifugation at 5,488x g for 40 min at 4°C (Heraeus™ Megafuge™ 40R) and incubated for 2 h with 500 μl HisPurTM Cobalt Resin (89964, ThermoFisher Scientific) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Neuroscience 2021Quote: ... The nuclei of astrocytes and autaptic neurons were visualized by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI)-containing mounting medium (ProLong® Gold Antifade Reagent with DAPI, Thermo Fisher Scientific). Only neurons with one nucleus were analyzed when counting synapse numbers.
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bioRxiv - Microbiology 2022Quote: ... 0.1% Triton X-100 in PBS for 20 minutes, then were actin stained with DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) nucleic acid stain (Thermo Fisher Scientific, USA) in PBS for 10 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were immersed in 30% sucrose at 4°C until sunk and flash frozen with cold 2-methylbutane (Fisher Scientific, Hampton, NH, USA). Coronal sections of the pregenual mPFC were obtained at 35 µm using a cryostat (Leica CM3050 S ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated at 4°C for 3 hours on a rotator before being placed in a DynaMag™-2 magnetic rack (Thermo Scientific, 12321D) and washed twice with TBS+NP40 wash buffer ...