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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... samples were washed with 1x PBS three times for 10 minutes and then incubated for one hour with secondary antibody (1:500 Goat anti-Rabbit Alexa Fluor 635, ThermoFisher, A-31577) in blocking buffer solution at room temperature ...
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bioRxiv - Immunology 2024Quote: ... the coupling procedure was performed as follows: 1 ×106 beads of one region were activated with 10 µL of 50 mg/mL EDC (Thermo Fisher Scientific) and 10 µL of 50 mg/mL s-NHS (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... 5% [v/v] glycerol) was concentrated to 9 mg/mL (measured by UV absorbance in a NanoDrop spectrophotometer (Thermo Scientific) using the calculated molecular weight and estimated extinction coefficient ...
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bioRxiv - Cancer Biology 2021Quote: PC-9 and HCC827 cell lines were cultured at 37C and 5% CO2 in RPMI 1640 GlutaMAX media (Gibco, US) supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2022Quote: ... and samples were centrifuged at 8000xg for 5 minutes at −9°C in a Sorvall Legend Micro 17R (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Immunology 2021Quote: ... The 96-well plates w preloaded with 9 μL of RT-STA solution: 5 μL of CellsDirect PCR mix (Invitrogen), 0.2 μL of SuperScript-III RT/Platinum Taq mix (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker and then incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker after which they were incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... PLAT-E cells were washed with 45 ml of PBS and were dissociated from the culture flask by a 5 minute incubation (37°C) with 9 ml of TrypLE reagent (Gibco), which was inactivated by adding 36 ml PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... brucei 427 parasites were grown at 37°C and 5% CO2 in HMI-9 medium supplemented with Penicillin-Streptomycin (Gibco) and 10% Fetal Bovine Serum (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... Established Schwann cells–DRG neuronal cocultures one week post dissection were infected with lentiviruses at an MOI ranging between 5 and 9 and incubated for 16 h in MEM (Invitrogen), 5% FBS (vol/vol ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells were pelleted by gentle centrifugation (500x g for 10 min at 4°C) in a Heraeus™ Megafuge™ 40R (ThermoFisher Scientific) and resuspended in a small volume of culture medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Tryptic peptides were labeled with isobaric Tandem Mass Tags (TMT) according to the manufacturer’s instructions using the TMT 10-Plex Kit or TMTpro 16-Plex Kit from Thermo Fisher (Supplemental Table 4). The TMT 10-Plex reagents and TMTpro 16-plex reagents were used for labeling samples from case 343WM the remaining nine cases ...
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bioRxiv - Developmental Biology 2021Quote: IP samples were boiled at 70°C for 10 minutes and separated on a 4-12% gradient Bis-Tris gel (Thermo Fisher Scientific, #NP0321) in 1x MOPS (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2019Quote: ... Mitochondria were carefully layered on top of the gradient and spun at 22,700 RPM for 10 min at 4°C in an ultracentrifuge (Thermo Scientific SureSpin 630 rotor). The purified mitochondrial fraction was collected at the 50% - 22% Percoll interface ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then fixed with 4% paraformaldehyde (PFA) for 10 min on ice and permeabilized with permeabilization buffer containing the intracellular stain cocktail (Invitrogen, Carlsbad, CA, USA) for 40 min ...
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bioRxiv - Developmental Biology 2021Quote: ... then denatured at 70°C for 10 minutes and loaded into 12-well NuPAGE Novex 4-12% Bis-Tris precast gels (Thermo Fisher Scientific – NP0322BOX). The pre-stained Novex Sharp protein standard (Invitrogen – LC5800 ...
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bioRxiv - Cell Biology 2021Quote: Samples were run in NuPAGE Bis-Tris gels (4%-12% [Invitrogen NP0321] for small volumes of samples, 10% [Invitrogen NP0315] for larger amounts) in 1x MOPS SDS running buffer (Invitrogen NP0001 ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated at 70°C for 10 min and separated on a Novex™ NuPAGE™ 4-12 % Bis-Tris Mini Protein Gel (Art. No. NP0321, Invitrogen™) in 1x Novex™ NuPAGE™ MOPS SDS Running Buffer (Art ...
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bioRxiv - Immunology 2021Quote: ... bone marrow was collected from femurs and tibia of BALB/c mice and then seeded into bacteriological Petri dishes at 4 × 106 in 10 ml of complete RPMI 1640 medium supplemented with 2mM L-glutamine (Life Technologies, Gaithersburg, MD), 100U/ml penicillin and 100μg/ml streptomycin ...
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bioRxiv - Biochemistry 2019Quote: ... The guard cartridge and analytical column used were the CarboPac PA1 (Thermo Scientific, 4 mm x 50 mm and 10 µm particle sizes) and CarboPac PA1 (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... the histological section immediately adjacent to each microbiopsy section were fixed in 4% PFA for 10 minutes and counter stained using 300 μM DAPI (Thermo Fisher, Cat. D1306) in 1x (pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... 10µg of protein was submitted to SDS-PAGE electrophoresis using NuPAGE Novex or Bolt 4-10% Bis-Tris gels (Thermo Fisher Scientific, USA). Proteins were transferred to nitrocellulose membranes using the iBlot Dry Blotting System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... SimplyBlue™ SafeStain and NuPAGE®Novex® 4-12% Bis-Tris Protein Gels (1.0 mm 10 well) were purchased from Life Technologies. Tris (hydroxymetthyl ...
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bioRxiv - Microbiology 2022Quote: Purified virions were heated at 95 °C for 10 min before separation on 4-12% Bis-Tris polyacrylamide gels (Thermo Fisher Scientific NW00125BOX) and transfer to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 65°C for 10 min followed by addition of 4 μL first strand buffer (Thermo Fisher Scientific Cat# 18064014), 2 μL 100 mM DTT (Thermo Fisher Scientific Cat# 18064014) ...
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bioRxiv - Neuroscience 2022Quote: ... they were washed three times in 0.1M PB (10 minutes per wash at 4°C) and then incubated with Alexa Fluor 594 or 633-conjugated streptavidin (Life technologies, 2mg/ml) in 0.1M PB containing 0.6% Triton X-100 ...
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bioRxiv - Plant Biology 2024Quote: 10 µg of plasmid DNA linearized using SpeI was transformed into electrocompetent PichiaPink™ strain 4 (ThermoFisher; P. pastoris genotype ade2, pep4, prb1) targeted to the TRP2 locus ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30 µg of protein was loaded onto precast gel (NP0321, NuPAGE™ 4- 12%, Bis-Tris, Mini Protein Gels, ThermoFisher Scientific, USA) and resolved at 135 volts for 90 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 in the Vitrobot setting) and plunge-frozen at 4°C under 95% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). The frozen grid was imaged using a Titan Krios G3 microscope at 300 kV equipped with a Falcon III detector ...
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bioRxiv - Cancer Biology 2024Quote: ... lysates were extracted with Laemelli sample buffer for 10 minutes at 75°C and lysates were run on 4% to 12% Bis-Tris gels (Invitrogen; Thermo Fisher Scientific). Proteins were transferred to nitrocellulose membranes (Prometheus ...
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bioRxiv - Microbiology 2024Quote: ... Filtered supernatant was concentrated approximately 10-fold by spinning at 4°C 2000 RCF for 30 min using a Pierce Protein Concentrator (ThermoFisher, Cat. No. 88537) and then virus aliquots were stored at −80°C and titrated as described in Crawford et al.73 The titers of the concentrated GPC-pseudotyped virus were typically in the range of 1.5×105 to 2×105 transduction units per mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed with 1x TBST (4 x 10 min) and processed using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific PI34080).
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bioRxiv - Molecular Biology 2023Quote: ... IMR90 EVs and concentrated CM from various cell lines were denatured at 70°C for 10 minutes and run on NuPAGE® Novex® 4-12% Bis-Tris gel system (Invitrogen, NP0321BOX). Gels were electroblotted onto the nitrocellulose membrane (Amersham Pharmacia Biotech) ...
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bioRxiv - Neuroscience 2023Quote: ... Lysates were cleared through centrifugation (14,000 g for 10 min at 4°C) and total protein content was quantified with a Bicinchoninic acid assay (BCA) Assay (Thermo Scientific Pierce, 23227) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... we washed the PA hydrogel three times with 1mL PBS and then stored it overnight at 4°C in PBS with 10% Antibiotic-Antimycotic 100X (Anti-Anti; Gibco, ThermoFisher, 15-240-062) and 1% bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2024Quote: ... PLL-coated dishes were then incubated overnight at 4°C with a 10 μg/mL solution of NeutrAvidin (Thermo Scientific, catalog no. 31000) in 1x Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were boiled at 95 °C for 10 min and run on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (Thermo Fisher Cat#NP0322BOX). Bands were visualized by staining the gel with Pierce GelCode blue stain reagent following manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... The dialyzed serum was subjected to 2 x centrifugation at 10,000 g for 10 min at 4°C and then filtered on a 0.45 μm Sterile Disposable Bottle Top Filters (Thermo Scientific Nalgene #295-4545). An affinity matrix was prepared by coupling 50 mg of His-tagged GST-RAP to 20 ml of NHS-activated Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were maintained in DMEM medium (for both H-4-II-E and HuH-7 cells) supplemented with 10% (v/v) fetal bovine serum (FBS; ThermoFisher, Waltham, MA, USA) and antibiotics (containing 10011U/mL penicillin and 10011μg/mL streptomycin ...
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bioRxiv - Biochemistry 2020Quote: ... One μL of each sample was diluted into 10 μL of HiDi formamide (ThermoFisher) containing 1 nM Cy3 internal standard DNA oligo (5’-[Cy3]-CCGTGAGTTGGTTGGACGGCTGCGAGGC-3’ ...
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bioRxiv - Cancer Biology 2020Quote: ... or 10% charcoal-stripped serum (CSS; One Shot Fetal Bovine Serum, Charcoal Strippped, Gibco) for androgen deprivation therapy response ...
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bioRxiv - Biochemistry 2019Quote: ... and eGFP and mCherry plasmids were transformed into One Shot Top 10 cells (Invitrogen). A single colony was used to inoculate a 10 mL 2x YT overnight culture supplemented with 200 μg/mL of Carbenicillin (Carb) ...
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bioRxiv - Cancer Biology 2021Quote: ... with 10% Charcoal/Dextran treated FBS (Charcoal STRP FBS One Shot, ThermoFisher Scientific, A3382101) and 2 mM L-glutamine to a concentration of 1 million cells per mL ...
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bioRxiv - Immunology 2020Quote: ... containing 10% human pooled serum (One Lambda, ThermoFisher Scientific Brand, West Hills CA, USA), 100 units/mL penicillin ...
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bioRxiv - Cancer Biology 2023Quote: One million cells were incubated with MitoTracker Deep Red (10 nM; Thermo Fisher Scientific) for 30 min in a humidified incubator maintained at 37°C ...
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bioRxiv - Systems Biology 2022Quote: ... supplemented with 10% FCS (BioConcept, 2-01F00-I) and 1% Penicillin/Streptomycin (Gibco, 15140-122) and kept at a confluency between 0.5 - 1.2 × 106 cells per ml at 37°C in a 5% CO2 incubator ...