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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Precipitated virus particles were pelleted by centrifugation at 1,600 x g for 1 hour at 4 °C and resuspended with DMEM (GIBCO). Concentrated virus particles were frozen and stored at − 80 °C until use.
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bioRxiv - Neuroscience 2021Quote: ... at 4°C for 1hr and 45 minutes in 1X NuPAGE® Transfer Buffer (Life Technologies, cat# NP0006-1). Blocking and antibody incubation was performed in 2% BSA (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with rabbit anti-ApoE antibody (1:200, #701241, Invitrogen, Carlsbad, CA, USA) and either rat anti-CD31 antibody (1:200 ...
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bioRxiv - Immunology 2020Quote: ... Baker) for 45 min at 4 °C and then permeabilized with either 1 × Permeabilization Buffer (transcriptional factor staining, ThermoFisher) or 1 × Perm/Wash Buffer (cytokine staining ...
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bioRxiv - Immunology 2021Quote: ... Blots were incubated overnight at 4°C with agitation in blocking buffer and anti-HisG-HRP (1:5000, Invitrogen), or anti-V5-HRP (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... CCR10 (248918) from R&D, CD11a (M17/4) from eBioscience, FcεRI (MAR-1), c-Kit (CD117, 2B8) from Invitrogen and Mouse BD Fc Block™ ...
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bioRxiv - Biophysics 2020Quote: ... hexanoyl)-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphoethanolamine) (Invitrogen). Cleavage of PED-6 eliminates a self-quenching effect that results in the release of a BODIPY-FL dye ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and with anti-rat IgG Alex Fluor 488 (ThermoFisher, Cat# A-11006, dilution 1:1000), anti-guinea pig IgG Alex Fluor 568 (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... within a biosafety cabinet SIS was cut into 1-inch segments and then incubated in 4 % ethanol (Fisher Scientific) and 0.1 % peracetic acid (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... 1:100 overnight at 4°C followed by Alexa Fluor 594 goat-anti rabbit secondary antibodies (Invitrogen, ON, Canada) at 1:400 for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... tissue was digested for a further 24 h with 0.5 mg/mL collagenase type IV (CLS-4, Lorne Laboratories) and 1 mg/mL dispase II (17105041, Invitrogen) at 37 °C and 5% CO2 with constant agitation [38].
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The muscle cells were centrifuged at 300g for 5 minutes and the cell pellet resuspended in Ham’s Complete growth medium (4% bovine calf serum [cytiva, Cat# SH30077.03], 1% penicillin and streptomycin [gibco by Life Technologies, Cat# 15140122] ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were incubated overnight at 4 °C with specific anti-FASN (#PA5-22061, Thermo Fisher Scientific; dilution 1:400) and anti-LAMP2 (#H4B4 ...
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bioRxiv - Neuroscience 2022Quote: 100 to 150 3-dpf zebrafish larvae were bathed in Yo-Pro-1 (4 µM, 30 min) (Invitrogen, Y3603), then washed 3 times in blue water to label lateral line hair cells ...
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bioRxiv - Microbiology 2023Quote: Cultured viruses were collected by centrifugation at 9,000 rpm for 1 h at 4°C (Sorvall ST8FR, Thermo Scientific) and resuspended in phosphate-buffered saline ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rat secondary antibody (1:1000, Life Technologies). Arteries were identified by immunostaining with a mouse monoclonal anti actin ...
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bioRxiv - Plant Biology 2022Quote: ... and qualified and quantified using 1% agarose gel and a Qubit™ Fluorometer 4 (Invitrogen, Life Technology, CA, USA). DNA was randomly fragmented using Bioruptor® Pico B01060010 (Diagenode ...
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bioRxiv - Neuroscience 2024Quote: ... confluent iPSC cultures were detached using 0.5mM EDTA and plated 1:4 on Matrigel ES-coated plates in StemFlex Medium (Gibco) supplemented with 10μM ROCK inhibitor (SellChem) ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then incubated at 4°C for 18 hours with secondary antibodies: DAPI (D1306, Life Technologies, 1:1000), AF488 (A11008 ...
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bioRxiv - Cell Biology 2024Quote: ... overnight incubation at 4°C was performed 1:500 dilution of goat anti-rabbit secondary antibody AlexaFluor 488 (Invitrogen) and goat anti-mouse secondary antibody AlexaFluor 488 (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were incubated overnight at 4 °C in a primary antibody solution (polyclonal rabbit anti-zo-1, Invitrogen Cat# 40-2200 ...
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bioRxiv - Neuroscience 2024Quote: ... incubated with the secondary antibody solution at 4 °C overnight (anti-mouse Alexa Fluor 488 (1:400; Invitrogen; A11001), anti-mouse Cy3 (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was centrifuged (158,000 g, 4 °C, 1 h) and the cleared lysate incubated with Ni-NTA agarose (Invitrogen) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Gels were then run at 4°C in 0.5X TBE before staining in 1/10000 SYBR Gold (ThermoFisher Scientific) for 1 hour at room temperature on a rocker and visualization on the Typhoon 9400 Variable Mode Imager using the A488 channel.
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bioRxiv - Biophysics 2023Quote: ... sonicated plasma membranes were fixed with 4% PFA before incubation with primary (GFP Thermo Fisher A11122 Rabbit 1/20) and secondary antibodies coupled to gold beads (rabbit-gold 815.011 AURION goat 1/25) ...
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bioRxiv - Bioengineering 2023Quote: ... monolayers reaching desired time-points were rinsed in 1× PBS followed by fixing in 4 % formaldehyde (28906, Thermofisher Scientific) for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% n-Dodecyl-β-D-maltoside were loaded onto Novex 4-16% Bis/Tris gradient gels (Life Technologies). When assaying stoichiometry of AMPAR-auxiliary subunit complexes ...
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bioRxiv - Cell Biology 2023Quote: ... was maintained in a medium composed of 4:1 of Dulbecco’s minimal essential medium (DMEM, Gibco, Invitrogen, NY, USA) and Medium 199 ...
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bioRxiv - Neuroscience 2023Quote: iPSC-derived neurons were washed with Krebs HEPES Buffer and incubated with 1 μm Fluo 4-AM (Thermofisher F14201) in Krebs HEPES Buffer for 20 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... at 4°C overnight and the secondary goat anti-rabbit-Alexa Fluor 488 antibody (1:300, Molecular Probes, A11008) at 37°C for 90 min ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Cell Biology 2023Quote: ... was maintained in a medium composed of 4:1 of Dulbecco’s minimal essential medium (DMEM, Gibco, Invitrogen, NY, USA) and Medium 199 ...
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bioRxiv - Molecular Biology 2023Quote: ... Brain slices were then incubated overnight at 4 °C with the rabbit anti-GFP primary antibody (1:1000, Invitrogen) diluted in blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... and cells were then incubated overnight at 4 °C with the rabbit anti-GFP primary antibody (1:1000, Invitrogen) diluted in 3 % BSA in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... mixed with PBS (4:1) adjusted to a concentration of 17,000 cells/µl and cultured in culture medium Advanced DMEM/F12 (Gibco), Primocin 50 µg/ml (Invivogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium was changed to differentiation medium containing DMEM/M199 at a 4:1 ratio (Gibco, 10566016 and 31150022), 20 mM Hepes (Biochrom ...
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bioRxiv - Immunology 2023Quote: ... and packaging vector psPAX2 were mixed in a 4:3:1 ratio in OPTI-MEM (Thermo Fisher Scientific, 31985070) and PEI (Polysciences ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM MnCl2 with a final assay concentration of 75 µM 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) (Invitrogen D6567). Generation of 6,8-difluro-4-methylumbelliferone (DiFMU ...
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bioRxiv - Immunology 2023Quote: ... sequences of HLA-A*02:01:01:01 exons 1-4 (GenBank no. HG794376.1) were synthesized (Thermo Fisher Scientific) and cloned into two previously described TÜ165-CAR-epHIV7 vectors by using NheI and RsrII restriction sites to ...
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bioRxiv - Neuroscience 2023Quote: ... and were incubated overnight at 4°C in polyclonal rabbit IgG fraction anti-Lucifer yellow (1:500, Life Technologies - Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4#x00B0;C with rabbit anti-mCherry primary antibody (1:200, Invitrogen, #PA5-34974). Sections were then washed with PBS and incubated for 2 hr at room temperature with a secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in 0.1M PBS again with the same sequence and incubated with agitation in the dark for 2 hrs in 0.4% Triton-X in 0.1M PBS with secondary antibodies: goat anti-chicken IgG (Alexa Fluor 488; 1:600; Invitrogen) and for Fos detection ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×105 HEK 293T cells were plated on each respective well of a 4 well chamber slide (Thermofisher Scientific) and incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... monolayers reaching desired time-points were rinsed in 1× PBS followed by fixing in 4 % formaldehyde (28906, Thermofisher Scientific) for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... purified cells were plated in 96-well round bottom plate in Yssel’s media (IMDM + 4% v/v Yssel’s supplement (made in house, Amsterdam UMC) + 1% v/v human AB serum (Invitrogen)) ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by SDS-PAGE gel electrophoresis on 1 mM NuPAGE 4 – 12% bis-tris gels (Thermo Fisher) using MOPS running buffer and transferred to nitrocellulose membranes using Tris-Glycine-methanol transfer buffer (30.3 g Tris Base ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was stained using the respective antibody (MGMT: 1:500 dilution, 4°C overnight incubation, #MA5-13506, Invitrogen, Waltham ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed at 4 °C for Scribble and CHIKV proteins using anti-Scrib (1:500, Invitrogen PA5-54821), anti-E2 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... cells were plated at 1×106 in 4 10cm plates 24 hours prior to harvest through trypsinization (ThermoFisher Scientific) for adherent cells ...
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bioRxiv - Developmental Biology 2024Quote: Tissue samples were fixed overnight at +4°C in periodate–lysine–paraformaldehyde (PLP) buffer [1% paraformaldehyde (Thermo Scientific, 28908), 0.01M sodium periodate (Sigma ...