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Citations for Thermo Fisher :
5851 - 5900 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 primary antibody overnight at 4°C and a AlexaFluor goat anti-rabbit secondary antibody (ThermoFisher, A-11008) at 1:500 for 4 hours at RT with PBS washes in between antibody steps.
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bioRxiv - Neuroscience 2024Quote: ... slices were washed and stained for 1-4 hours with goat anti-chicken Alexa Fluor 488 (A-11039, Invitrogen) secondary solution ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg or 4 µg of modRNA was mixed with Opti-MEM reduced serum medium plus GlutaMAX (ThermoFisher Scientific) and Lipofectamine RNAiMAX (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... MDA-MB-231 cells were co-transfected with pXPR_001-sgRab21 and pUC57-Rab21 HDR plasmids in 1:4 ratio using Lipofectamine 3000 (Invitrogen). Five days post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with secondary antibodies in vehicle overnight at 4 °C (Alexa flor 488 goat anti mouse, Invitrogen A11001, 1:1000; Alexa flor 546 goat anti rabbit, Invitrogen A11035, 1:500). After 3 washes for 30 minutes in PBS ...
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bioRxiv - Immunology 2021Quote: ... The phage/serum mixtures were rotated overnight at 4°C after which 40 μL of a 1:1 Protein A/G coated magnetic bead mixture (Invitrogen, #10002D and #10004D) was added to each well and rotated for an additional 4 hours at 4°C to capture all IgG ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated with anti-KLK10 (BiossUSA bs-2531R, 1:100) primary antibody overnight at 4°C followed by Alexa Fluor-647 (ThermoFisher Scientific, 1:500) secondary antibody for 2hrs at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were preincubated in 3% Bovine serum albumin-PBS at room temperature for 1 h and incubated overnight at 4°C with primary antibodies against CC-ORF (rabbit anti-GFP, polyclonal, Invitrogen A11122, 1:1000). The sections were then incubated with the corresponding fluorescein-conjugated secondary antibodies (Alexa Fluor 488 anti-rabbit ...
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bioRxiv - Developmental Biology 2023Quote: ... -GFAP (1:500, SynapticSystems) -Aquaporin-4 (1:200, Alomone Labs) as well as with Click-iT™ EdU Cell Proliferation Kit (Thermo Fisher Scientific) according to manufactures protocol ...
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bioRxiv - Microbiology 2023Quote: ... cells were rinsed again thrice with 1×PBS before an overnight blocking stage at 4°C in 1×SEA BLOCK blocking buffer (Thermo Fisher Scientific, Poland).
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bioRxiv - Cell Biology 2024Quote: ... 3D or in suspension as described above were incubated with cell-impermeable 4 µM ethidium homodimer 1 (EthD-1; Invitrogen catalogue no. E1169) and 10 µg/ml cell-permeable Hoechst (Thermo Fisher catalogue no ...
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bioRxiv - Cell Biology 2024Quote: ... then stained for 1 h at 4°C in PBS with 1:1000 Alexa-647 conjugated M1 mouse anti-FLAG antibody (Invitrogen, Cat #MA1-91878). Cells were moved to flow cytometry (BD FACS Canto2) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h and incubated overnight at 4°C with the following primary antibodies: anti-GFP (Thermo Fisher Scientific, A-11122, 1:1000), anti-TIAR (BD Transduction ...
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bioRxiv - Neuroscience 2020Quote: ... samples were run at 1:5 or 1:10 dilution with a working concentration of 10 uM primers and Fast SYBR green master mix (Applied Biosystems, 4385610). Ct values were transformed to ΔΔCt values by normalizing to 18s and WT samples ...
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bioRxiv - Genetics 2022Quote: ... The 10 μL Hifi Cas9 protein were aliquoted into 10 tubes followed by 1:5 dilution with Opti-MEM (ThermoFisher Scientific, 31985062) solution before use.
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bioRxiv - Immunology 2021Quote: Tendon samples were homogenized for 45 seconds at 4 m/s in 600 μL TRIzol reagent using a Fisherbrand™ Bead Mill 4 Homogenizer (Thermo Fisher Scientific, 15-340-164) with reinforced 2 mL tubes (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... the cells were stained with CD3 BUV805 or CD3 AF700 (4:100 or 4:100; BD Biosciences Cat# 612895 or Life Technologies Cat# 56-0038-42, respectively), CD4 BV605 (4:100 ...
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bioRxiv - Neuroscience 2019Quote: ... 10-12 ml of the nonionic detergent-based lysis reagent B-PER (Thermo Scientific #78266) in phosphate buffer containing 1mg/ml of lysozyme (Thermo Scientific #89833) ...
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bioRxiv - Cancer Biology 2021Quote: ... NSB-75 and peripheral blood B cells (PBBC) were cultured in RPMI+10% FBS (Gibco), supplemented with 2mM L-glutamine (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... colonies were selected on YPD containing 300 µg/ml hygromycin B (Gibco 10-687-010).
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... We performed confocal reflectance microscopy on unlabeled fibrin samples (see Fig. 2) and fluorescence imaging (see Fig. 4) on networks polymerized from unlabeled fibrinogen and Alexa488-labeled human fibrinogen (Life Technologies, Bleiswijk, the Netherlands) mixed in a 30:1 molar ratio.
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bioRxiv - Cell Biology 2020Quote: ... Nucleoporin-specific antibody was used for primary staining (over-night incubation at 4°C) and and AlexaFluor-conjugated secondary antibodies (Invitrogen; 2 hours at RT). Microscopy was performed as previously described 16 ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Microbiology 2021Quote: ... Healthy cell culture of the primmorphs (2–4 mm in diameter) of green color were transferred to the 24-well plates (Nalge Nunc International, Rochester, NY, USA), 1 pieces per well ...
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific, Basel, Switzerland). The beads were washes 5 times with lysis buffer and proteins were eluted in TEV buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... A baculovirus stock carrying the gene for DNMT1 was used to infect 4–12 l of Sf9 cells at a density of 2 million cells/ml in Sf-900™ III SFM (Invitrogen cat # 12658–027). Multiple 1 l cell cultures in 4 l flasks (VWR cat # 32645–044 ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared lysates were incubated for 2 h at 4°C with prewashed anti-c-myc MagBeads (Pierce Thermo Fisher Scientific, product number 88843). After five washes with lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: ... acutely dissociated DRG neurons from 4 mice were first incubated in at 37 °C for 30 minutes in 4 mm Fura-2-acetoxymethyl ester and 0.2% Pluronic® F127 (Thermo Fisher Scientific, Waltham, MA), washed three times ...
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bioRxiv - Cancer Biology 2023Quote: ... Tris-Glycine 10-20% or Bis-Tris 4-12% SDS-PAGE gels were run at 4℃ and proteins were transferred onto nitrocellulose membranes using 15 Volts for 7 minutes via the iBlot-2 system (Thermo Fisher Scientific, Waltham, MA). The membrane was then blocked for 1 hour in LICOR Odyssey blocking buffer and incubated at 4℃ with the appropriate antibody overnight ...
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bioRxiv - Microbiology 2023Quote: ... Cells from two separate transects were counted from a 4-well dish with inverted light microscope (Zeiss PrimoVert) after 2 h of settling (Nunc, Thermo Fisher Scientific, US). Depending on the culture density up to 1 L of sample was filtered through each (n = 5 per sampling day ...
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bioRxiv - Cancer Biology 2023Quote: ... Next day the cells were washed with fresh media and incubated at 37°C for 45 min in culture media containing 4 μM Fura-2/acetoxymethyl ester (Molecular Probes, Eugene, OR, USA). Then the cells were washed and kept in HEPES-buffered saline solution (140 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Immunology 2024Quote: ... differentiated Th2 cells were restimulated with anti-mouse CD3 (4 µg/mL) for 3 hours followed by 2 hours of incubation with monensin (Thermo Fisher Scientific, MA, USA) at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with 0.5 µM Lipi-Deep Red neutral lipid stain (Dojindo #LD04-10) for 2 hr and 5 µg/mL Hoeschst 33342 nucleic acid stain (Invitrogen #H3570) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... EdU pulse-labelling was performed at the indicated time points by 30 min incubation in the presence of 10 µM 5-ethynyl-2-deoxyuridine (Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... 5×107 mouse ESCs (untreated and OHT-treated) were crosslinked in 10 ml 1x PBS with 2 mM DSG (Thermo Scientific) for 45 minutes at 25°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, ThermoFisher) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cancer Biology 2023Quote: ... SCC25-Luc-GFP cells co-cultured with mouse or naked mole-rat fibroblasts were incubated with 10 µM 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher) for 1.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the nucleus was counterstained with 0.5 µg/mL 4’6-diamidino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Prolong® Gold (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... Cells obtained from fractions 2–5 were combined according to the genotype and expanded in α-MEM containing 10% FBS (Gibco), 100 U/ml penicillin ...
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bioRxiv - Plant Biology 2024Quote: In situ hybridization experiments were performed on 2 - 5 mm ear primordia and 10-21 day old seedling apexes fixed in PFA (Thermo Scientific), dehydrated in a graded ethanol series ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.0) and 2 µg anti-5-methylcytosine (5-mC) antibody (Thermo Fisher, 33D3), and incubated at 4°C overnight with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... 5% CO2 in poly-D-Ornithine coated 48-well plates (9-10×104 cells/well in 0.5 mL) and grown in supplemented DMEM SILAC medium (Gibco), containing Glucose 2.5 mM ...
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bioRxiv - Cell Biology 2020Quote: ... with one wash containing DAPI (1:50000, D3571, Invitrogen, Thermofisher), mounted with mouting media and stored in darkness at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... with one wash containing DAPI (1:50000, D3571, Invitrogen, Thermofisher), mounted with mouting media and stored in darkness at 4°C ...