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Citations for Thermo Fisher :
5651 - 5700 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: All RNA samples were quantified and followed by cDNA synthesis using a First-Strand cDNA Synthesis Kit for Real-Time PCR (USB-Affymetrix, Santa Clara, CA, USA; #75780). Samples were prepared according to manufacturer’s instructions for a 20-µL reaction ...
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bioRxiv - Cell Biology 2020Quote: ... and quantified by qPCR with the quantification kit (Tian Gen company, China) for Illumina with QuantStudio 12K Flex Real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA). Libraries were diluted to 2 nM in each and pooled with equal volume before sequenced on Hiseq X Ten (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA was used for real-time PCR with three multiplex wells once with TaqMan gene assay kit (Thermo Fisher Scientific, Applied Biosystems, Carlsbad, CA, USA). The results were normalized to GAPDH ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: Candidate SNP genotyping was performed using TaqMan® Genotyping Assay kit on ABI 7500 Real-Time PCR System from Applied Biosystems (Foster City, CA, USA). Each polymerase chain reaction sample was composed of 10 ng of DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR for quantification was run using an Illumina-compatible KAPA Library Quantification Kits (ROX Low) (cat# KK4873) on an Applied Biosystems ViiA 7 Real-Time qPCR / PCR Thermal Cycler System (Applied Biosystems, San Francisco, CA, USA) and was analyzed using QuantStudio software (RRID ...
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bioRxiv - Plant Biology 2019Quote: ... qRT-PCR amplification was performed using the specific primer pairs (Table S12) on a 7500 real time PCR system (Applied Biosystems Inc., USA). The amplification process was performed according the method of Suo et al ...
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bioRxiv - Molecular Biology 2020Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described97 ...
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bioRxiv - Cell Biology 2019Quote: ... reverse-transcribed and quantitative PCR was performed using Fast SYBR Green Master Mix in a Viia7 Real-Time PCR system (Applied Biosystems, Warrington, UK). Human primers were pre-validated Quantitect primers (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulted cDNA was used to perform quantitative PCR in an Applied Biosystems Step One Plus real-time PCR system (Applied Biosystems, CA, USA) using SYBR Green PCR Master Mix (Takara ...
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bioRxiv - Immunology 2020Quote: Quantitative PCR (qPCR) was performed using ViiA 7 Real-Time PCR System and Fast SYBR Green Master Mix reagent (Applied Biosystems, Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1).
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed on a QuantStudio 6 Flex Real-Time PCR System using SYBR Green Power SYBR™ Green PCR-Master-Mix (Applied Biosystems™) and primers listed in Supplementary Table 2.
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bioRxiv - Bioengineering 2021Quote: ... and the solution was added to a Custom RT2 PCR Array 384-well plate and qPCR program was run using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems 4485701). Data analysis was performed on https://geneglobe.qiagen.com/us/analyze ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR to check expression of transcript levels was performed using StepOneTM Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA) with the following programme ...
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bioRxiv - Microbiology 2020Quote: ... Real-time qualitative PCR analysis was performed as described previously (9) using an Applied Sciences StepOne Plus Real Time PCR System (Life Technologies, Carlsbad, CA). Primer and probe sequences used for gene expression analysis are listed in Supplementary Table S2 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR reaction was performed in a QuantStudio™ 6 Flex Real-Time PCR system instrument (384-well, Applied Biosystems, Foster City, CA) with an initial incubation at 95°C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: Quantitative PCR was performed from the immunoprecipitated DNA or the whole cell extract using a 7900HT Fast Real-Time PCR System and SYBR Green PCR master mix (Applied Biosystems, Thermo Scientific) as described (23) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Standard Fast TaqMan PCR amplification was performed in triplicate on a Step One Plus Real-Time PCR System (Applied Biosystems; Grand Island, NY) in a final volume of 20 µL containing ∼10 ng of cDNA (1.5 µL of RT product) ...
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bioRxiv - Microbiology 2022Quote: ... Real-time qualitative PCR analysis was performed as described previously(42) using an Applied Sciences Step One Plus Real Time PCR System (Life Technologies, Carlsbad, CA).Primers and probes were designed using IDT and are mentioned in supplementary material (Table:S1) ...
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bioRxiv - Physiology 2021Quote: ... Hypothalamic gene expression levels were analyzed by quantitative PCR using a StepOnePlus Real-Time PCR System (Applied Biosystems, Inc. Foster City, CA, USA). elongation factor 1 alpha (ef1α ...
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bioRxiv - Molecular Biology 2022Quote: ... Gene expression analysis was then performed by quantitative PCR on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems, Foster City, CA). PCR cycling ...
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
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bioRxiv - Neuroscience 2019Quote: ... Quantitative real-time polymerase chain reaction (qPCR) analysis was performed on an Applied Biosystems™ StepOnePlus™ Real-Time PCR Systems using Power SYBRGreen PCR Master Mix (Applied Biosystems) or TaqMan™ Gene Expression Master Mix (Applied Biosystems™) ...
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bioRxiv - Neuroscience 2020Quote: ... The presence of the SARS-CoV-2 in these samples was evaluated by one-step qRT-PCR in a final volume of 25 μL per reaction in 96-well PCR plates using a thermocycler (7500t Real-time PCR system, Applied Biosystems, Applied Biosystems). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: Relative mRNA expression was determined by quantitative PCR (qPCR) using SYBR Green Real-Time PCR Master Mix (Applied Biosystems, Foster City, CA, USA). Total RNA was isolated from soleus muscle (10 µg ...
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bioRxiv - Genetics 2019Quote: ... cDNA was amplified on an Applied Biosystems ViiA7 Real-Time PCR System with Power SYBR Green PCR Master Mix (ThermoFisher Scientific, Waltham, MA). GAPDH was used as control ...
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bioRxiv - Genomics 2021Quote: qRT-PCR was performed using Quantstudio 3 Real-Time PCR system and SYBR green master mixture (Thermo Fisher Scientific, USA Catalog number: A25778). All gene expression levels were normalized to Rpl32 (in Drosophila) ...
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bioRxiv - Physiology 2020Quote: ... Gene expression analysis was then performed by quantitative PCR on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems, Foster City, CA) using the mouse primers shown in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR were performed with TaqMan Gene Expression Assays (Applied Biosystem, Waltham, MA) on a StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA). Relative target gene expression levels were calculated using the delta-delta CT method(Livak & Schmittgen ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was done in triplicate with the QuantStudio 5 Real-time PCR system and Fast SYBR® Green Master Mix (Thermo Fisher Scientific) with 500 nM primers ...
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bioRxiv - Genomics 2022Quote: ... The product was diluted 1:2 in RNase-free water and subjected to quantitative real-time PCR (qRT-PCR) using gene-specific TaqMan assays (Thermo Fisher Scientific 4331182) for mouse TTR (Mm00443267_m1) ...
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bioRxiv - Systems Biology 2022Quote: ... The bacterial load present in the purified samples was measured by quantitative PCR (StepOnePlus Real-Time PCR System, Thermo Fisher Scientific, the Netherlands), using a universal primer set targeting the 16S rRNA gene (forward Eub34lF ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was purified using a QIAquick PCR Purification kit and qPCR was performed with Power Up SyBr Green PCR master mix using the ABI 7900HT Real Time PCR system (Applied Biosystems, Waltham, MA). PCR reactions contained primers for murine Vcan Prom400 (mVCAN Prom 400 ...
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bioRxiv - Biochemistry 2024Quote: ... Real-Time Quantitative PCR (qPCR) was performed with the standard protocols in ABI 7500 Fast Real-Time PCR System (Applied Biosystems™ 7500, ThermoFisher, USA) using SYBR Green Master Mix ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... qRT-PCR was performed using the PowerUp SyBr Green Master Mix on the QuantStudio™ 3 Real-Time PCR System (Applied Biosystems, Thermo Scientific). Amplification was performed using the rapid cycling mode following the manufacturer’s specifications.The primers were designed based on nucleic acid sequences obtained from the National Center for Biotechnology Information and the primer sequences are displayed in Supplementary Table S1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative RT-PCR was performed with TaqMan Gene Expression Assays (Applied Biosystem, Waltham, MA) on an StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA). Relative target gene expression levels were calculated using the delta-delta CT method ...
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bioRxiv - Bioengineering 2022Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time polymerase chain reaction (PCR) was performed using the Vazyme System (Vazyme, Nanjing, China) on a 7900HT FAST Real-time PCR System (Life Technologies, Carlsbad, CA). The level of genes was calculated based on the cycle threshold (Ct ...
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bioRxiv - Neuroscience 2023Quote: ... Transcript levels of candidate genes were measured by qRT-PCR using cDNA with the QuantStudio 6 Flex Real-Time PCR System (Life Technologies, Darmstadt, Germany) according to the company’s guidelines.
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bioRxiv - Genetics 2024Quote: ... and TaqMan Assay probes were loaded in technical quadruplicates for qRT-PCR on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, 4485691). The ΔΔCt method was used to provide gene expression values after normalizing to the known reference gene Gapdh ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cDNA was used to quantify the SARS-CoV-2 RNA levels by real-time quantitative PCR using Power SYBR green PCR master mix (Applied Biosystems, Waltham, MA). Average values from duplicates of each sample were used to calculate the viral RNA level relative to the GAPDH gene and presented as 2−ΔCT ...
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bioRxiv - Cell Biology 2024Quote: ... was performed on 12 ng cDNA with SybrGreen PCR mastermix (LifeTechnologies) and specific primers (supplementary Table 2) and run in an ABI? real-time PCR machine (Applied Biosystems, ThermoFisher Scientific). The raw data was analysed through -ΔCt as described in Supplementary Methods.
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bioRxiv - Biochemistry 2024Quote: ... was performed on the cDNA (diluted 1 in 5 in nuclease-free water) using the Power SYBR Green PCR Master Mix and the StepOnePlus Real-Time PCR System (both Applied Biosystems, Warrington, UK). MALAT1 expression was normalised to RPL10 (60S ribosomal protein L10 ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was carried out using THUNDERBIRD SYBR qPCR Mix (TOYOBO, Osaka, Japan) on 7300 Real-Time PCR System (Applied Biosystems, Massachusetts, USA). For normalization ...
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bioRxiv - Genomics 2024Quote: ... cDNA was diluted 10-fold and subjected to quantitative PCR using the PrimeTime™ Gene Expression Master Mix (Integrated DNA Technologies, Inc.) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). Primers and probes (Integrated DNA Technologies ...
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bioRxiv - Genomics 2021Quote: ... and the hrdB gene was used as the endogenous control (StepOne Plus real-time PCR system, Applied Biosystems). The sequences of optimized oligonucleotides used in this study are listed in Supplementary Table 2).
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bioRxiv - Cancer Biology 2021Quote: ... Amplification was monitored using SYBR Green dye on a QuantStudio™ 3 Real-Time PCR System machine (ThermoFisher). All measurements were performed in triplicate and calculated using the ΔΔct method relative to GAPDH expression.
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bioRxiv - Cell Biology 2020Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). The primer sequence for specific transcripts were as followed ...
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bioRxiv - Cell Biology 2020Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). The primer sequence for specific transcripts were as followed ...
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bioRxiv - Cell Biology 2020Quote: ... was used and qPCR was performed on a StepOnePlus Real time PCR System (Applied Biosystems, Foster City, CA). Primers for qPCR:
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bioRxiv - Immunology 2021Quote: ... with 0.8 μM of forward and reverse primers on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) with the following cycling conditions ...